Human Gene STRADA (uc002jbm.3) Description and Page Index
  Description: Homo sapiens STE20-related kinase adaptor alpha (STRADA), transcript variant 1, mRNA.
RefSeq Summary (NM_001003787): The protein encoded by this gene contains a STE20-like kinase domain, but lacks several residues that are critical for catalytic activity, so it is termed a 'pseudokinase'. The protein forms a heterotrimeric complex with serine/threonine kinase 11 (STK11, also known as LKB1) and the scaffolding protein calcium binding protein 39 (CAB39, also known as MO25). The protein activates STK11 leading to the phosphorylation of both proteins and excluding STK11 from the nucleus. The protein is necessary for STK11-induced G1 cell cycle arrest. A mutation in this gene has been shown to result in polyhydramnios, megalencephaly, and symptomatic epilepsy (PMSE) syndrome. Multiple transcript variants encoding different isoforms have been found for this gene. Additional transcript variants have been described but their full-length nature is not known. [provided by RefSeq, Sep 2009]. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: SRR3476690.671002.1, SRR3476690.1104067.1 [ECO:0000332] RNAseq introns :: mixed/partial sample support SAMEA1965299, SAMEA1966682 [ECO:0000350] ##Evidence-Data-END## ##RefSeq-Attributes-START## MANE Ensembl match :: ENST00000336174.12/ ENSP00000336655.6 RefSeq Select criteria :: based on conservation, expression, longest protein ##RefSeq-Attributes-END##
Transcript (Including UTRs)
   Position: hg19 chr17:61,780,192-61,819,330 Size: 39,139 Total Exon Count: 13 Strand: -
Coding Region
   Position: hg19 chr17:61,780,959-61,805,729 Size: 24,771 Coding Exon Count: 12 

Page IndexSequence and LinksUniProtKB CommentsMalaCardsCTDGene Alleles
RNA-Seq ExpressionMicroarray ExpressionRNA StructureProtein StructureOther SpeciesGO Annotations
mRNA DescriptionsPathwaysOther NamesModel InformationMethods
Data last updated: 2013-06-14

-  Sequence and Links to Tools and Databases
 
Genomic Sequence (chr17:61,780,192-61,819,330)mRNA (may differ from genome)Protein (431 aa)
Gene SorterGenome BrowserOther Species FASTAVisiGeneGene interactionsTable Schema
BioGPSCGAPEnsemblEntrez GeneExonPrimerGeneCards
H-INVHGNCHPRDLynxMGIneXtProt
OMIMPubMedReactomeStanford SOURCETreefamUniProtKB
Wikipedia

-  Comments and Description Text from UniProtKB
  ID: STRAA_HUMAN
DESCRIPTION: RecName: Full=STE20-related kinase adapter protein alpha; Short=STRAD alpha; AltName: Full=STE20-related adapter protein; AltName: Full=Serologically defined breast cancer antigen NY-BR-96;
FUNCTION: Pseudokinase which, in complex with CAB39/MO25 (CAB39/MO25alpha or CAB39L/MO25beta), binds to and activates STK11/LKB1. Adopts a closed conformation typical of active protein kinases and binds STK11/LKB1 as a pseudosubstrate, promoting conformational change of STK11/LKB1 in an active conformation.
SUBUNIT: Component of a trimeric complex composed of STK11/LKB1, STRAD (STRADA or STRADB) and CAB39/MO25 (CAB39/MO25alpha or CAB39L/MO25beta): the complex tethers STK11/LKB1 in the cytoplasm and stimulates its catalytic activity.
INTERACTION: Q15831:STK11; NbExp=9; IntAct=EBI-1109114, EBI-306838;
SUBCELLULAR LOCATION: Nucleus. Cytoplasm.
DOMAIN: The protein kinase domain is predicted to be catalytically inactive.
DISEASE: Note=A homozygous 7-kb deletion involving STRADA is a cause of a syndrome characterized by polyhydramnios, megalencephaly and symptomatic epilepsy.
SIMILARITY: Belongs to the protein kinase superfamily. STE Ser/Thr protein kinase family. STE20 subfamily.
SIMILARITY: Contains 1 protein kinase domain.

-  MalaCards Disease Associations
  MalaCards Gene Search: STRADA
Diseases sorted by gene-association score: polyhydramnios, megalencephaly, and symptomatic epilepsy* (941), polyhydramnios (45), megalencephaly (17), scheuermann disease (16), uterine disease (1)
* = Manually curated disease association

-  Comparative Toxicogenomics Database (CTD)
  The following chemicals interact with this gene           more ... click here to view the complete list

+  Common Gene Haplotype Alleles
  Press "+" in the title bar above to open this section.

-  RNA-Seq Expression Data from GTEx (53 Tissues, 570 Donors)
  Highest median expression: 7.27 RPKM in Testis
Total median expression: 151.76 RPKM



View in GTEx track of Genome Browser    View at GTEx portal     View GTEx Body Map

+  Microarray Expression Data
  Press "+" in the title bar above to open this section.

-  mRNA Secondary Structure of 3' and 5' UTRs
 
RegionFold EnergyBasesEnergy/Base
Display As
5' UTR -122.50274-0.447 Picture PostScript Text
3' UTR -271.08767-0.353 Picture PostScript Text

The RNAfold program from the Vienna RNA Package is used to perform the secondary structure predictions and folding calculations. The estimated folding energy is in kcal/mol. The more negative the energy, the more secondary structure the RNA is likely to have.

-  Protein Domain and Structure Information
  InterPro Domains: Graphical view of domain structure
IPR011009 - Kinase-like_dom
IPR000719 - Prot_kinase_cat_dom

Pfam Domains:
PF00069 - Protein kinase domain
PF07714 - Protein tyrosine kinase

SCOP Domains:
56112 - Protein kinase-like (PK-like)

Protein Data Bank (PDB) 3-D Structure
MuPIT help

1UPK
- X-ray MuPIT

2WTK
- X-ray MuPIT

3GNI
- X-ray MuPIT


ModBase Predicted Comparative 3D Structure on Q7RTN6
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The pictures above may be empty if there is no ModBase structure for the protein. The ModBase structure frequently covers just a fragment of the protein. You may be asked to log onto ModBase the first time you click on the pictures. It is simplest after logging in to just click on the picture again to get to the specific info on that model.

-  Orthologous Genes in Other Species
  Orthologies between human, mouse, and rat are computed by taking the best BLASTP hit, and filtering out non-syntenic hits. For more distant species reciprocal-best BLASTP hits are used. Note that the absence of an ortholog in the table below may reflect incomplete annotations in the other species rather than a true absence of the orthologous gene.
MouseRatZebrafishD. melanogasterC. elegansS. cerevisiae
No orthologGenome BrowserNo orthologNo orthologNo orthologNo ortholog
Gene DetailsGene Details    
Gene SorterGene Sorter    
 RGD    
 Protein Sequence    
 Alignment    

-  Gene Ontology (GO) Annotations with Structured Vocabulary
  Molecular Function:
GO:0000166 nucleotide binding
GO:0005515 protein binding
GO:0005524 ATP binding
GO:0019900 kinase binding
GO:0030295 protein kinase activator activity
GO:0043539 protein serine/threonine kinase activator activity
GO:0004672 protein kinase activity

Biological Process:
GO:0006611 protein export from nucleus
GO:0007049 cell cycle
GO:0007050 cell cycle arrest
GO:0007346 regulation of mitotic cell cycle
GO:0031098 stress-activated protein kinase signaling cascade
GO:0032147 activation of protein kinase activity
GO:0042981 regulation of apoptotic process
GO:0071902 positive regulation of protein serine/threonine kinase activity
GO:0006468 protein phosphorylation

Cellular Component:
GO:0005634 nucleus
GO:0005654 nucleoplasm
GO:0005737 cytoplasm
GO:0005829 cytosol


-  Descriptions from all associated GenBank mRNAs
  LF384811 - JP 2014500723-A/192314: Polycomb-Associated Non-Coding RNAs.
BC043641 - Homo sapiens STE20-related kinase adaptor alpha, mRNA (cDNA clone MGC:10181 IMAGE:3908187), complete cds.
AL832407 - Homo sapiens mRNA; cDNA DKFZp667F1913 (from clone DKFZp667F1913).
AK128173 - Homo sapiens cDNA FLJ46296 fis, clone TESTI4035770.
AK074771 - Homo sapiens cDNA FLJ90290 fis, clone NT2RP1001020, weakly similar to SERINE/THREONINE-PROTEIN KINASE PAK-BETA (EC 2.7.1.-).
AK075005 - Homo sapiens cDNA FLJ90524 fis, clone NT2RP4000962, weakly similar to SPORULATION-SPECIFIC PROTEIN 1 (EC 2.7.1.-).
AY290821 - Homo sapiens LYK5 protein mRNA, complete cds.
AF308302 - Homo sapiens serologically defined breast cancer antigen NY-BR-96 mRNA, complete cds.
JD525073 - Sequence 506097 from Patent EP1572962.
JD398863 - Sequence 379887 from Patent EP1572962.
JD283895 - Sequence 264919 from Patent EP1572962.
JD296504 - Sequence 277528 from Patent EP1572962.
JD058075 - Sequence 39099 from Patent EP1572962.
JD173812 - Sequence 154836 from Patent EP1572962.
JD460274 - Sequence 441298 from Patent EP1572962.
JD225727 - Sequence 206751 from Patent EP1572962.
JD345844 - Sequence 326868 from Patent EP1572962.
JD431746 - Sequence 412770 from Patent EP1572962.
JD133390 - Sequence 114414 from Patent EP1572962.
JD036895 - Sequence 17919 from Patent EP1572962.
JD127856 - Sequence 108880 from Patent EP1572962.
JD401542 - Sequence 382566 from Patent EP1572962.
JD199049 - Sequence 180073 from Patent EP1572962.
JD470933 - Sequence 451957 from Patent EP1572962.
JD332920 - Sequence 313944 from Patent EP1572962.
JD139969 - Sequence 120993 from Patent EP1572962.
JD150413 - Sequence 131437 from Patent EP1572962.
JD214758 - Sequence 195782 from Patent EP1572962.
JD251086 - Sequence 232110 from Patent EP1572962.
JD063801 - Sequence 44825 from Patent EP1572962.
JD400510 - Sequence 381534 from Patent EP1572962.
JD372420 - Sequence 353444 from Patent EP1572962.
AK309283 - Homo sapiens cDNA, FLJ99324.
AK293160 - Homo sapiens cDNA FLJ59453 complete cds, highly similar to Homo sapiens protein kinase LYK5 (LYK5), transcript variant 1, mRNA.
JD394878 - Sequence 375902 from Patent EP1572962.
JD383342 - Sequence 364366 from Patent EP1572962.
AK301331 - Homo sapiens cDNA FLJ56897 complete cds, highly similar to Homo sapiens protein kinase LYK5 (LYK5), transcript variant 1, mRNA.
JD248309 - Sequence 229333 from Patent EP1572962.
JD253765 - Sequence 234789 from Patent EP1572962.
HQ257904 - Synthetic construct Homo sapiens clone IMAGE:100072213 Unknown protein gene, encodes complete protein.
KJ903585 - Synthetic construct Homo sapiens clone ccsbBroadEn_12979 STRADA gene, encodes complete protein.
KJ905591 - Synthetic construct Homo sapiens clone ccsbBroadEn_15217 STRADA gene, encodes complete protein.
DQ595043 - Homo sapiens piRNA piR-61155, complete sequence.
LF328232 - JP 2014500723-A/135735: Polycomb-Associated Non-Coding RNAs.
JD019456 - Sequence 480 from Patent EP1572962.
JD030743 - Sequence 11767 from Patent EP1572962.
LF328233 - JP 2014500723-A/135736: Polycomb-Associated Non-Coding RNAs.
MA620388 - JP 2018138019-A/192314: Polycomb-Associated Non-Coding RNAs.
MA563809 - JP 2018138019-A/135735: Polycomb-Associated Non-Coding RNAs.
MA563810 - JP 2018138019-A/135736: Polycomb-Associated Non-Coding RNAs.

-  Biochemical and Signaling Pathways
  KEGG - Kyoto Encyclopedia of Genes and Genomes
hsa04150 - mTOR signaling pathway

Reactome (by CSHL, EBI, and GO)

Protein Q7RTN6 (Reactome details) participates in the following event(s):

R-HSA-380942 LKB1 forms a trimeric complex with STRAD and MO25
R-HSA-200421 Activation of cytosolic AMPK by phosphorylation
R-HSA-380972 Energy dependent regulation of mTOR by LKB1-AMPK
R-HSA-165159 mTOR signalling
R-HSA-162582 Signal Transduction

-  Other Names for This Gene
  Alternate Gene Symbols: B4DDE3, LYK5, NM_001003787, NP_001003788, Q5JPI2, Q7RTN6, Q7Z4K9, Q8NC31, Q8NCF1, Q9H272, STRAA_HUMAN, STRAD
UCSC ID: uc002jbm.3
RefSeq Accession: NM_001003787
Protein: Q7RTN6 (aka STRAA_HUMAN)
CCDS: CCDS32703.1

-  Gene Model Information
 
category: coding nonsense-mediated-decay: no RNA accession: NM_001003787.2
exon count: 13CDS single in 3' UTR: no RNA size: 2367
ORF size: 1296CDS single in intron: no Alignment % ID: 100.00
txCdsPredict score: 2557.00frame shift in genome: no % Coverage: 98.73
has start codon: yes stop codon in genome: no # of Alignments: 1
has end codon: yes retained intron: no # AT/AC introns 0
selenocysteine: no end bleed into intron: 0# strange splices: 0
Click here for a detailed description of the fields of the table above.

-  Methods, Credits, and Use Restrictions
  Click here for details on how this gene model was made and data restrictions if any.