Human Gene TRIM45 (ENST00000256649.9) from GENCODE V44
  Description: Homo sapiens tripartite motif containing 45 (TRIM45), transcript variant 1, mRNA. (from RefSeq NM_025188)
RefSeq Summary (NM_025188): This gene encodes a member of the tripartite motif family. The encoded protein may function as a transcriptional repressor of the mitogen-activated protein kinase pathway. Alternatively spliced transcript variants have been described.[provided by RefSeq, Mar 2009].
Gencode Transcript: ENST00000256649.9
Gencode Gene: ENSG00000134253.10
Transcript (Including UTRs)
   Position: hg38 chr1:117,111,060-117,121,749 Size: 10,690 Total Exon Count: 6 Strand: -
Coding Region
   Position: hg38 chr1:117,112,305-117,121,201 Size: 8,897 Coding Exon Count: 6 

Page IndexSequence and LinksUniProtKB CommentsPrimersCTDRNA-Seq Expression
Microarray ExpressionRNA StructureProtein StructureOther SpeciesGO AnnotationsmRNA Descriptions
PathwaysOther NamesMethods
Data last updated at UCSC: 2023-08-18 00:09:47

-  Sequence and Links to Tools and Databases
 
Genomic Sequence (chr1:117,111,060-117,121,749)mRNA (may differ from genome)Protein (580 aa)
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HGNCHPRDLynxMalacardsMGIneXtProt
OMIMPubMedReactomeUniProtKBWikipediaBioGrid CRISPR DB

-  Comments and Description Text from UniProtKB
  ID: TRI45_HUMAN
DESCRIPTION: RecName: Full=Tripartite motif-containing protein 45; AltName: Full=RING finger protein 99;
FUNCTION: May act as a transcriptional repressor in mitogen- activated protein kinase signaling pathway.
SUBCELLULAR LOCATION: Cytoplasm. Nucleus.
TISSUE SPECIFICITY: Expressed in skeletal muscle, brain, heart and pancreas.
DEVELOPMENTAL STAGE: Expressed in brain, lung, skeletal muscle, heart and intestine in 80-day old embryo.
SIMILARITY: Belongs to the TRIM/RBCC family.
SIMILARITY: Contains 2 B box-type zinc fingers.
SIMILARITY: Contains 1 filamin repeat.
SIMILARITY: Contains 1 RING-type zinc finger.

-  Primer design for this transcript
 

Primer3Plus can design qPCR Primers that straddle exon-exon-junctions, which amplify only cDNA, not genomic DNA.
Click here to load the transcript sequence and exon structure into Primer3Plus

Exonprimer can design one pair of Sanger sequencing primers around every exon, located in non-genic sequence.
Click here to open Exonprimer with this transcript

To design primers for a non-coding sequence, zoom to a region of interest and select from the drop-down menu: View > In External Tools > Primer3


-  Comparative Toxicogenomics Database (CTD)
  The following chemicals interact with this gene

-  RNA-Seq Expression Data from GTEx (53 Tissues, 570 Donors)
  Highest median expression: 7.16 RPKM in Pituitary
Total median expression: 135.76 RPKM



View in GTEx track of Genome Browser    View at GTEx portal     View GTEx Body Map

+  Microarray Expression Data
  Press "+" in the title bar above to open this section.

-  mRNA Secondary Structure of 3' and 5' UTRs
 
RegionFold EnergyBasesEnergy/Base
Display As
5' UTR -253.00548-0.462 Picture PostScript Text
3' UTR -333.201245-0.268 Picture PostScript Text

The RNAfold program from the Vienna RNA Package is used to perform the secondary structure predictions and folding calculations. The estimated folding energy is in kcal/mol. The more negative the energy, the more secondary structure the RNA is likely to have.

-  Protein Domain and Structure Information
  InterPro Domains: Graphical view of domain structure
IPR001298 - Filamin
IPR017868 - Filamin/ABP280_repeat-like
IPR013783 - Ig-like_fold
IPR014756 - Ig_E-set
IPR000315 - Znf_B-box
IPR018957 - Znf_C3HC4_RING-type
IPR001841 - Znf_RING
IPR013083 - Znf_RING/FYVE/PHD
IPR017907 - Znf_RING_CS

Pfam Domains:
PF00630 - Filamin/ABP280 repeat
PF00643 - B-box zinc finger
PF00097 - Zinc finger, C3HC4 type (RING finger)

Protein Data Bank (PDB) 3-D Structure
MuPIT help
2DS4 - NMR MuPIT


ModBase Predicted Comparative 3D Structure on Q9H8W5
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-  Orthologous Genes in Other Species
  Orthologies between human, mouse, and rat are computed by taking the best BLASTP hit, and filtering out non-syntenic hits. For more distant species reciprocal-best BLASTP hits are used. Note that the absence of an ortholog in the table below may reflect incomplete annotations in the other species rather than a true absence of the orthologous gene.
MouseRatZebrafishD. melanogasterC. elegansS. cerevisiae
Genome BrowserGenome BrowserGenome BrowserGenome BrowserGenome BrowserNo ortholog
Gene Details     
Gene Sorter     
MGIRGDEnsemblEnsemblWormBase 
Protein SequenceProtein SequenceProtein SequenceProtein SequenceProtein Sequence 
AlignmentAlignmentAlignmentAlignmentAlignment 

-  Gene Ontology (GO) Annotations with Structured Vocabulary
  Molecular Function:
GO:0008270 zinc ion binding
GO:0046872 metal ion binding

Biological Process:
GO:0060348 bone development

Cellular Component:
GO:0005622 intracellular
GO:0005634 nucleus
GO:0005654 nucleoplasm
GO:0005737 cytoplasm
GO:0005829 cytosol
GO:0045171 intercellular bridge


-  Descriptions from all associated GenBank mRNAs
  BC034943 - Homo sapiens tripartite motif-containing 45, mRNA (cDNA clone MGC:26026 IMAGE:4826874), complete cds.
AY669488 - Homo sapiens tripartite motif-containing 45 (TRIM45) mRNA, complete cds.
AK222901 - Homo sapiens mRNA for tripartite motif-containing 45 variant, clone: HRC03950.
AK023243 - Homo sapiens cDNA FLJ13181 fis, clone NT2RP3004016, weakly similar to TRANSCRIPTION INTERMEDIARY FACTOR 1-BETA.
JD558636 - Sequence 539660 from Patent EP1572962.
EU447017 - Synthetic construct Homo sapiens clone IMAGE:100070142; IMAGE:100012226; FLH257436.01L tripartite motif-containing 45 (TRIM45) gene, encodes complete protein.
DQ574574 - Homo sapiens piRNA piR-42686, complete sequence.
JD207148 - Sequence 188172 from Patent EP1572962.
JD327064 - Sequence 308088 from Patent EP1572962.
JD175085 - Sequence 156109 from Patent EP1572962.
JD204280 - Sequence 185304 from Patent EP1572962.
JD395878 - Sequence 376902 from Patent EP1572962.
JD111627 - Sequence 92651 from Patent EP1572962.
JD424806 - Sequence 405830 from Patent EP1572962.
JD474596 - Sequence 455620 from Patent EP1572962.
JD485851 - Sequence 466875 from Patent EP1572962.
JD166969 - Sequence 147993 from Patent EP1572962.

-  Biochemical and Signaling Pathways
  Reactome (by CSHL, EBI, and GO)

Protein Q9H8W5 (Reactome details) participates in the following event(s):

R-HSA-877300 Interferon gamma signaling
R-HSA-913531 Interferon Signaling
R-HSA-1280215 Cytokine Signaling in Immune system
R-HSA-168256 Immune System

-  Other Names for This Gene
  Alternate Gene Symbols: ENST00000256649.1, ENST00000256649.2, ENST00000256649.3, ENST00000256649.4, ENST00000256649.5, ENST00000256649.6, ENST00000256649.7, ENST00000256649.8, NM_025188, Q53GN0, Q5T2K4, Q5T2K5, Q8IYV6, Q9H8W5, RNF99, TRI45_HUMAN, uc001egz.1, uc001egz.2, uc001egz.3, uc001egz.4
UCSC ID: ENST00000256649.9
RefSeq Accession: NM_025188
Protein: Q9H8W5 (aka TRI45_HUMAN)
CCDS: CCDS893.1, CCDS44200.1

-  Methods, Credits, and Use Restrictions
  Click here for details on how this gene model was made and data restrictions if any.