Human Gene MCM10 (ENST00000484800.6) from GENCODE V44
Description: Homo sapiens minichromosome maintenance 10 replication initiation factor (MCM10), transcript variant 1, mRNA. (from RefSeq NM_182751) RefSeq Summary (NM_182751): The protein encoded by this gene is one of the highly conserved mini-chromosome maintenance proteins (MCM) that are involved in the initiation of eukaryotic genome replication. The hexameric protein complex formed by MCM proteins is a key component of the pre-replication complex (pre-RC) and it may be involved in the formation of replication forks and in the recruitment of other DNA replication related proteins. This protein can interact with MCM2 and MCM6, as well as with the origin recognition protein ORC2. It is regulated by proteolysis and phosphorylation in a cell cycle-dependent manner. Studies of a similar protein in Xenopus suggest that the chromatin binding of this protein at the onset of DNA replication is after pre-RC assembly and before origin unwinding. Alternatively spliced transcript variants encoding distinct isoforms have been identified. [provided by RefSeq, Jul 2008]. Gencode Transcript: ENST00000484800.6 Gencode Gene: ENSG00000065328.17 Transcript (Including UTRs) Position: hg38 chr10:13,161,579-13,209,736 Size: 48,158 Total Exon Count: 20 Strand: + Coding Region Position: hg38 chr10:13,164,203-13,209,310 Size: 45,108 Coding Exon Count: 19
ID:MCM10_HUMAN DESCRIPTION: RecName: Full=Protein MCM10 homolog; Short=HsMCM10; FUNCTION: Acts as a replication initiation factor that brings together the MCM2-7 helicase and the DNA polymerase alpha/primase complex in order to initiate DNA replication. Additionally, plays a role in preventing DNA damage during replication. SUBUNIT: Self-associates (By similarity). Interacts with ORC2. May interact with MCM2 and MCM6. Interacts with the DNA polymerase alpha subunit POLA1. Interacts with RECQL4; this interaction regulates RECQL4 unwinding activity. Interacts with WDHD1. INTERACTION: Q14566:MCM6; NbExp=2; IntAct=EBI-374912, EBI-374900; Q13416:ORC2; NbExp=5; IntAct=EBI-374912, EBI-374957; SUBCELLULAR LOCATION: Nucleus. Note=Colocalizes with ORC2 in nuclei foci. Associated with chromatin in S phase. From early to mid-S phase located in discrete nuclear foci. In early S phase, several hundred foci appeared throughout the nucleus. In mid-S phase, the foci appeared at the nuclear periphery and nucleolar regions. In the late S and G phases localized to nucleoli. DEVELOPMENTAL STAGE: Expression is cell cycle regulated. Expression increases at the G1/S-boundary. Expression decreases in late M phase, remains low during G(1) phase, and starts to accumulate at the onset of S phase. DOMAIN: Each zinc finger-like domain binds a zinc ion and is involved in both ssDNA and dsDNA binding, as is the OB-fold domain (By similarity). DOMAIN: The N-terminal domain mediates homodimerization (By similarity). PTM: Phosphorylated upon DNA damage, probably by ATM or ATR. SIMILARITY: Belongs to the MCM10 family. SEQUENCE CAUTION: Sequence=AAF69623.1; Type=Erroneous initiation;
The RNAfold program from the Vienna RNA Package is used to perform the secondary structure predictions and folding calculations. The estimated folding energy is in kcal/mol. The more negative the energy, the more secondary structure the RNA is likely to have.
ModBase Predicted Comparative 3D Structure on Q7L590
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Orthologous Genes in Other Species
Orthologies between human, mouse, and rat are computed by taking the best BLASTP hit, and filtering out non-syntenic hits. For more distant species reciprocal-best BLASTP hits are used. Note that the absence of an ortholog in the table below may reflect incomplete annotations in the other species rather than a true absence of the orthologous gene.
AK292701 - Homo sapiens cDNA FLJ77264 complete cds, highly similar to Homo sapiens MCM10 minichromosome maintenance deficient 10 (MCM10), transcript variant 1, mRNA. AK055695 - Homo sapiens cDNA FLJ31133 fis, clone IMR322000973, weakly similar to DNA43 PROTEIN. BC101727 - Homo sapiens minichromosome maintenance complex component 10, mRNA (cDNA clone MGC:126776 IMAGE:8069233), complete cds. AB042719 - Homo sapiens mRNA for MCM10 homolog, complete cds. AL136840 - Homo sapiens mRNA; cDNA DKFZp434H152 (from clone DKFZp434H152). BC143490 - Homo sapiens minichromosome maintenance complex component 10, mRNA (cDNA clone MGC:177015 IMAGE:9051998), complete cds. BC009108 - Homo sapiens cDNA clone IMAGE:3451214. BC004876 - Homo sapiens minichromosome maintenance complex component 10, mRNA (cDNA clone IMAGE:3688557), partial cds. AF119869 - Homo sapiens PRO2249 mRNA, complete cds. KJ902823 - Synthetic construct Homo sapiens clone ccsbBroadEn_12217 MCM10 gene, encodes complete protein. JD359378 - Sequence 340402 from Patent EP1572962. JD281188 - Sequence 262212 from Patent EP1572962. JD134508 - Sequence 115532 from Patent EP1572962. DQ599364 - Homo sapiens piRNA piR-37430, complete sequence. JD082771 - Sequence 63795 from Patent EP1572962. JD311085 - Sequence 292109 from Patent EP1572962. JD092800 - Sequence 73824 from Patent EP1572962. JD490293 - Sequence 471317 from Patent EP1572962. JD063534 - Sequence 44558 from Patent EP1572962.
Biochemical and Signaling Pathways
Reactome (by CSHL, EBI, and GO)
Protein Q7L590 (Reactome details) participates in the following event(s):
R-HSA-68919 Mcm10 associates with the pre-replicative complex, stabilizing Mcm2-7 R-HSA-68940 Cdt1 is displaced from the pre-replicative complex. R-HSA-68918 CDK and DDK associate with the Mcm10:pre-replicative complex R-HSA-68917 Cdc45 associates with the pre-replicative complex at the origin R-HSA-68916 DNA Replication Factor A (RPA) associates with the pre-replicative complex at the origin R-HSA-176318 Loading of claspin onto DNA during replication origin firing R-HSA-176298 Activation of claspin R-HSA-68962 Activation of the pre-replicative complex R-HSA-69002 DNA Replication Pre-Initiation R-HSA-69206 G1/S Transition R-HSA-68874 M/G1 Transition R-HSA-453279 Mitotic G1-G1/S phases R-HSA-176187 Activation of ATR in response to replication stress R-HSA-69278 Cell Cycle (Mitotic) R-HSA-69306 DNA Replication R-HSA-69481 G2/M Checkpoints R-HSA-1640170 Cell Cycle R-HSA-69620 Cell Cycle Checkpoints
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