Human Gene DGKZ (ENST00000454345.6) from GENCODE V44
  Description: Homo sapiens diacylglycerol kinase zeta (DGKZ), transcript variant 4, mRNA. (from RefSeq NM_001105540)
RefSeq Summary (NM_001105540): The protein encoded by this gene belongs to the eukaryotic diacylglycerol kinase family. It may attenuate protein kinase C activity by regulating diacylglycerol levels in intracellular signaling cascade and signal transduction. Alternative splicing occurs at this locus and multiple transcript variants encoding distinct isoforms have been identified. [provided by RefSeq, Nov 2010].
Gencode Transcript: ENST00000454345.6
Gencode Gene: ENSG00000149091.15
Transcript (Including UTRs)
   Position: hg38 chr11:46,361,606-46,380,551 Size: 18,946 Total Exon Count: 32 Strand: +
Coding Region
   Position: hg38 chr11:46,366,257-46,379,947 Size: 13,691 Coding Exon Count: 31 

Page IndexSequence and LinksUniProtKB CommentsPrimersCTDRNA-Seq Expression
Microarray ExpressionRNA StructureProtein StructureOther SpeciesGO AnnotationsmRNA Descriptions
PathwaysOther NamesMethods
Data last updated at UCSC: 2023-08-18 00:09:47

-  Sequence and Links to Tools and Databases
Genomic Sequence (chr11:46,361,606-46,380,551)mRNA (may differ from genome)Protein (1117 aa)
Gene SorterGenome BrowserOther Species FASTAGene interactionsTable SchemaAlphaFold
BioGPSEnsemblEntrez GeneExonPrimerGeneCardsHGNC
WikipediaBioGrid CRISPR DB

-  Comments and Description Text from UniProtKB
DESCRIPTION: RecName: Full=Diacylglycerol kinase zeta; Short=DAG kinase zeta; EC=; AltName: Full=Diglyceride kinase zeta; Short=DGK-zeta;
FUNCTION: Displays a strong preference for 1,2-diacylglycerols over 1,3-diacylglycerols, but lacks substrate specificity among molecular species of long chain diacylglycerols. Isoform 2 but not isoform 1 regulates RASGRP1 activity.
CATALYTIC ACTIVITY: ATP + 1,2-diacylglycerol = ADP + 1,2-diacyl- sn-glycerol 3-phosphate.
SUBUNIT: Interacts with the PDZ domain of the syntrophin SNTG1 and that of SNX27. Isoform 2 forms a signaling complex with RASGRP1 and HRAS.
INTERACTION: P06400:RB1; NbExp=6; IntAct=EBI-715527, EBI-491274; P28749:RBL1; NbExp=2; IntAct=EBI-715527, EBI-971402; Q08999:RBL2; NbExp=2; IntAct=EBI-715527, EBI-971439;
SUBCELLULAR LOCATION: Cytoplasm. Nucleus. Cell membrane.
TISSUE SPECIFICITY: Highest levels in brain, and substantial levels in skeletal muscle, heart, and pancreas. Isoform 1 is predominantly expressed in muscle.
PTM: Phosphorylation of the MARCKS homology domain by PKC reduces nuclear accumulation of DGK-zeta.
SIMILARITY: Belongs to the eukaryotic diacylglycerol kinase family.
SIMILARITY: Contains 2 ANK repeats.
SIMILARITY: Contains 1 DAGKc domain.
SIMILARITY: Contains 2 phorbol-ester/DAG-type zinc fingers.
SEQUENCE CAUTION: Sequence=AAB60859.1; Type=Frameshift; Positions=166, 167;

-  Primer design for this transcript

Primer3Plus can design qPCR Primers that straddle exon-exon-junctions, which amplify only cDNA, not genomic DNA.
Click here to load the transcript sequence and exon structure into Primer3Plus

Exonprimer can design one pair of Sanger sequencing primers around every exon, located in non-genic sequence.
Click here to open Exonprimer with this transcript

To design primers for a non-coding sequence, zoom to a region of interest and select from the drop-down menu: View > In External Tools > Primer3

-  Comparative Toxicogenomics Database (CTD)
  The following chemicals interact with this gene           more ... click here to view the complete list

-  RNA-Seq Expression Data from GTEx (53 Tissues, 570 Donors)
  Highest median expression: 74.67 RPKM in Brain - Cortex
Total median expression: 869.69 RPKM

View in GTEx track of Genome Browser    View at GTEx portal     View GTEx Body Map

+  Microarray Expression Data
  Press "+" in the title bar above to open this section.

-  mRNA Secondary Structure of 3' and 5' UTRs
RegionFold EnergyBasesEnergy/Base
Display As
5' UTR -35.70114-0.313 Picture PostScript Text
3' UTR -295.90604-0.490 Picture PostScript Text

The RNAfold program from the Vienna RNA Package is used to perform the secondary structure predictions and folding calculations. The estimated folding energy is in kcal/mol. The more negative the energy, the more secondary structure the RNA is likely to have.

-  Protein Domain and Structure Information
  InterPro Domains: Graphical view of domain structure
IPR002110 - Ankyrin_rpt
IPR020683 - Ankyrin_rpt-contain_dom
IPR000756 - Diacylglycerol_kin_accessory
IPR001206 - Diacylglycerol_kinase_cat_dom
IPR002219 - Prot_Kinase_C-like_PE/DAG-bd

Pfam Domains:
PF00023 - Ankyrin repeat
PF00130 - Phorbol esters/diacylglycerol binding domain (C1 domain)
PF00609 - Diacylglycerol kinase accessory domain
PF00781 - Diacylglycerol kinase catalytic domain

ModBase Predicted Comparative 3D Structure on Q13574
The pictures above may be empty if there is no ModBase structure for the protein. The ModBase structure frequently covers just a fragment of the protein. You may be asked to log onto ModBase the first time you click on the pictures. It is simplest after logging in to just click on the picture again to get to the specific info on that model.

-  Orthologous Genes in Other Species
  Orthologies between human, mouse, and rat are computed by taking the best BLASTP hit, and filtering out non-syntenic hits. For more distant species reciprocal-best BLASTP hits are used. Note that the absence of an ortholog in the table below may reflect incomplete annotations in the other species rather than a true absence of the orthologous gene.
MouseRatZebrafishD. melanogasterC. elegansS. cerevisiae
Genome BrowserGenome BrowserGenome BrowserNo orthologGenome BrowserNo ortholog
Gene Details     
Gene Sorter     
MGIRGDEnsembl WormBase 
Protein SequenceProtein SequenceProtein Sequence Protein Sequence 
AlignmentAlignmentAlignment Alignment 

-  Gene Ontology (GO) Annotations with Structured Vocabulary
  Molecular Function:
GO:0000166 nucleotide binding
GO:0001727 lipid kinase activity
GO:0003951 NAD+ kinase activity
GO:0004143 diacylglycerol kinase activity
GO:0005515 protein binding
GO:0005524 ATP binding
GO:0008022 protein C-terminus binding
GO:0016301 kinase activity
GO:0016740 transferase activity
GO:0046872 metal ion binding

Biological Process:
GO:0007165 signal transduction
GO:0007205 protein kinase C-activating G-protein coupled receptor signaling pathway
GO:0008152 metabolic process
GO:0016310 phosphorylation
GO:0016477 cell migration
GO:0030168 platelet activation
GO:0031571 mitotic G1 DNA damage checkpoint
GO:0035556 intracellular signal transduction
GO:0045930 negative regulation of mitotic cell cycle
GO:0046339 diacylglycerol metabolic process
GO:0046486 glycerolipid metabolic process
GO:0046834 lipid phosphorylation

Cellular Component:
GO:0005634 nucleus
GO:0005737 cytoplasm
GO:0005886 plasma membrane
GO:0016020 membrane
GO:0016607 nuclear speck
GO:0030027 lamellipodium

-  Descriptions from all associated GenBank mRNAs
  AK124594 - Homo sapiens cDNA FLJ42603 fis, clone BRACE3011421, highly similar to Diacylglycerol kinase, zeta (EC
AK126970 - Homo sapiens cDNA FLJ45023 fis, clone BRAWH3017180, highly similar to Diacylglycerol kinase, zeta (EC
AK303322 - Homo sapiens cDNA FLJ53420 complete cds, highly similar to Diacylglycerol kinase zeta (EC
AK123378 - Homo sapiens cDNA FLJ41384 fis, clone BRCAN2014602, moderately similar to DIACYLGLYCEROL KINASE, ZETA (EC
BX648433 - Homo sapiens mRNA; cDNA DKFZp686G19253 (from clone DKFZp686G19253).
AK300577 - Homo sapiens cDNA FLJ53489 complete cds, highly similar to Diacylglycerol kinase zeta (EC
AK225774 - Homo sapiens mRNA for diacylglycerol kinase, zeta 104kDa isoform 2 variant, clone: FCC132F09.
U51477 - Human diacylglycerol kinase zeta mRNA, complete cds.
BC041770 - Homo sapiens diacylglycerol kinase, zeta 104kDa, mRNA (cDNA clone MGC:26405 IMAGE:4811463), complete cds.
AK312398 - Homo sapiens cDNA, FLJ92730.
AK293615 - Homo sapiens cDNA FLJ59586 complete cds, highly similar to Diacylglycerol kinase zeta (EC
AK294888 - Homo sapiens cDNA FLJ60516 complete cds, highly similar to Diacylglycerol kinase zeta (EC
KJ897858 - Synthetic construct Homo sapiens clone ccsbBroadEn_07252 DGKZ gene, encodes complete protein.
U94905 - Human diacylglycerol kinase zeta mRNA, alternatively spliced, complete cds.
AB209635 - Homo sapiens mRNA for diacylglycerol kinase, zeta 104kDa isoform 3 variant protein.
BC008018 - Homo sapiens diacylglycerol kinase, zeta 104kDa, mRNA (cDNA clone IMAGE:3534542), partial cds.
LQ980387 - Sequence 33 from Patent WO2013050532.
AK124693 - Homo sapiens cDNA FLJ42703 fis, clone BRAMY3005932.
AK097960 - Homo sapiens cDNA FLJ40641 fis, clone THYMU2016968, highly similar to DIACYLGLYCEROL KINASE, ZETA (EC
BX649190 - Homo sapiens mRNA; cDNA DKFZp686J21237 (from clone DKFZp686J21237).
JD177908 - Sequence 158932 from Patent EP1572962.
JD237247 - Sequence 218271 from Patent EP1572962.
JD439641 - Sequence 420665 from Patent EP1572962.
JD129155 - Sequence 110179 from Patent EP1572962.
JD449412 - Sequence 430436 from Patent EP1572962.
JD218379 - Sequence 199403 from Patent EP1572962.
JD066159 - Sequence 47183 from Patent EP1572962.
JD214492 - Sequence 195516 from Patent EP1572962.
JD393544 - Sequence 374568 from Patent EP1572962.
JD104513 - Sequence 85537 from Patent EP1572962.
JD335765 - Sequence 316789 from Patent EP1572962.
JD199924 - Sequence 180948 from Patent EP1572962.
JD156017 - Sequence 137041 from Patent EP1572962.
JD439797 - Sequence 420821 from Patent EP1572962.

-  Biochemical and Signaling Pathways
  Reactome (by CSHL, EBI, and GO)

Protein Q13574 (Reactome details) participates in the following event(s):

R-HSA-426240 DAG kinase produces phosphatidic acid from DAG
R-HSA-114508 Effects of PIP2 hydrolysis
R-HSA-76002 Platelet activation, signaling and aggregation
R-HSA-416476 G alpha (q) signalling events
R-HSA-109582 Hemostasis
R-HSA-388396 GPCR downstream signalling
R-HSA-372790 Signaling by GPCR
R-HSA-162582 Signal Transduction

-  Other Names for This Gene
  Alternate Gene Symbols: DAGK6, DGKZ_HUMAN, ENST00000454345.1, ENST00000454345.2, ENST00000454345.3, ENST00000454345.4, ENST00000454345.5, NM_001105540, O00542, Q13574, Q6ZVG7, uc001ncn.1, uc001ncn.2
UCSC ID: ENST00000454345.6
RefSeq Accession: NM_001105540
Protein: Q13574 (aka DGKZ_HUMAN)

-  Methods, Credits, and Use Restrictions
  Click here for details on how this gene model was made and data restrictions if any.