Human Gene MTNR1B (ENST00000257068.3) from GENCODE V44
  Description: Homo sapiens melatonin receptor 1B (MTNR1B), mRNA. (from RefSeq NM_005959)
RefSeq Summary (NM_005959): This gene encodes one of two high affinity forms of a receptor for melatonin, the primary hormone secreted by the pineal gland. This gene product is an integral membrane protein that is a G-protein coupled, 7-transmembrane receptor. It is found primarily in the retina and brain although this detection requires RT-PCR. It is thought to participate in light-dependent functions in the retina and may be involved in the neurobiological effects of melatonin. [provided by RefSeq, Jul 2008].
Gencode Transcript: ENST00000257068.3
Gencode Gene: ENSG00000134640.3
Transcript (Including UTRs)
   Position: hg38 chr11:92,969,651-92,982,782 Size: 13,132 Total Exon Count: 2 Strand: +
Coding Region
   Position: hg38 chr11:92,969,726-92,982,312 Size: 12,587 Coding Exon Count: 2 

Page IndexSequence and LinksUniProtKB CommentsPrimersMalaCardsCTD
RNA-Seq ExpressionMicroarray ExpressionRNA StructureProtein StructureOther SpeciesGO Annotations
mRNA DescriptionsPathwaysOther NamesMethods
Data last updated at UCSC: 2023-08-18 00:09:47

-  Sequence and Links to Tools and Databases
Genomic Sequence (chr11:92,969,651-92,982,782)mRNA (may differ from genome)Protein (362 aa)
Gene SorterGenome BrowserOther Species FASTAGene interactionsTable SchemaAlphaFold
BioGPSEnsemblEntrez GeneExonPrimerGencodeGeneCards
OMIMPubMedReactomeUniProtKBWikipediaBioGrid CRISPR DB

-  Comments and Description Text from UniProtKB
DESCRIPTION: RecName: Full=Melatonin receptor type 1B; Short=Mel-1B-R; Short=Mel1b receptor;
FUNCTION: High affinity receptor for melatonin. Likely to mediates the reproductive and circadian actions of melatonin. The activity of this receptor is mediated by pertussis toxin sensitive G proteins that inhibit adenylate cyclase activity.
SUBCELLULAR LOCATION: Cell membrane; Multi-pass membrane protein.
TISSUE SPECIFICITY: Expressed in retina and less in brain and hippocampus.
SIMILARITY: Belongs to the G-protein coupled receptor 1 family.
WEB RESOURCE: Name=Wikipedia; Note=Melatonin receptor entry; URL="";

-  Primer design for this transcript

Primer3Plus can design qPCR Primers that straddle exon-exon-junctions, which amplify only cDNA, not genomic DNA.
Click here to load the transcript sequence and exon structure into Primer3Plus

Exonprimer can design one pair of Sanger sequencing primers around every exon, located in non-genic sequence.
Click here to open Exonprimer with this transcript

To design primers for a non-coding sequence, zoom to a region of interest and select from the drop-down menu: View > In External Tools > Primer3

-  MalaCards Disease Associations
  MalaCards Gene Search: MTNR1B
Diseases sorted by gene-association score: diabetes mellitus, noninsulin-dependent* (101), idiopathic scoliosis (34), adolescent idiopathic scoliosis (11), scoliosis (3)
* = Manually curated disease association

-  Comparative Toxicogenomics Database (CTD)
  The following chemicals interact with this gene           more ... click here to view the complete list

-  RNA-Seq Expression Data from GTEx (53 Tissues, 570 Donors)
  Highest median expression: 0.29 RPKM in Testis
Total median expression: 0.42 RPKM

View in GTEx track of Genome Browser    View at GTEx portal     View GTEx Body Map

+  Microarray Expression Data
  Press "+" in the title bar above to open this section.

-  mRNA Secondary Structure of 3' and 5' UTRs
RegionFold EnergyBasesEnergy/Base
Display As
5' UTR -37.0075-0.493 Picture PostScript Text
3' UTR -183.10470-0.390 Picture PostScript Text

The RNAfold program from the Vienna RNA Package is used to perform the secondary structure predictions and folding calculations. The estimated folding energy is in kcal/mol. The more negative the energy, the more secondary structure the RNA is likely to have.

-  Protein Domain and Structure Information
  InterPro Domains: Graphical view of domain structure
IPR000276 - 7TM_GPCR_Rhodpsn
IPR017452 - GPCR_Rhodpsn_supfam
IPR002278 - Mel_1A_rcpt
IPR000025 - Melatonin_rcpt

Pfam Domains:
PF00001 - 7 transmembrane receptor (rhodopsin family)

ModBase Predicted Comparative 3D Structure on P49286
The pictures above may be empty if there is no ModBase structure for the protein. The ModBase structure frequently covers just a fragment of the protein. You may be asked to log onto ModBase the first time you click on the pictures. It is simplest after logging in to just click on the picture again to get to the specific info on that model.

-  Orthologous Genes in Other Species
  Orthologies between human, mouse, and rat are computed by taking the best BLASTP hit, and filtering out non-syntenic hits. For more distant species reciprocal-best BLASTP hits are used. Note that the absence of an ortholog in the table below may reflect incomplete annotations in the other species rather than a true absence of the orthologous gene.
MouseRatZebrafishD. melanogasterC. elegansS. cerevisiae
Genome BrowserGenome BrowserNo orthologNo orthologNo orthologNo ortholog
Gene Details     
Gene Sorter     
Protein SequenceProtein Sequence    

-  Gene Ontology (GO) Annotations with Structured Vocabulary
  Molecular Function:
GO:0004871 signal transducer activity
GO:0004930 G-protein coupled receptor activity
GO:0005515 protein binding
GO:0008502 melatonin receptor activity

Biological Process:
GO:0007165 signal transduction
GO:0007186 G-protein coupled receptor signaling pathway
GO:0007187 G-protein coupled receptor signaling pathway, coupled to cyclic nucleotide second messenger
GO:0007268 chemical synaptic transmission
GO:0007623 circadian rhythm
GO:0042593 glucose homeostasis
GO:0050796 regulation of insulin secretion

Cellular Component:
GO:0005886 plasma membrane
GO:0005887 integral component of plasma membrane
GO:0016020 membrane
GO:0016021 integral component of membrane

-  Descriptions from all associated GenBank mRNAs
  BC069163 - Homo sapiens melatonin receptor 1B, mRNA (cDNA clone MGC:95407 IMAGE:7216982), complete cds.
JD483360 - Sequence 464384 from Patent EP1572962.
JD538190 - Sequence 519214 from Patent EP1572962.
U25341 - Human Mel1b-melatonin receptor (MTNR1B) mRNA, complete cds.
AY521019 - Homo sapiens melatonin receptor 1B (MTNR1B) mRNA, complete cds.
AY114100 - Homo sapiens melatonin receptor 1B variant b (MTNR1B) mRNA, partial cds; alternatively spliced.
AF467654 - Homo sapiens melatonin receptor MEL1B (MTNR1B) mRNA, partial cds.
JD106728 - Sequence 87752 from Patent EP1572962.
JD468321 - Sequence 449345 from Patent EP1572962.
JD172660 - Sequence 153684 from Patent EP1572962.
JD095460 - Sequence 76484 from Patent EP1572962.
JD447811 - Sequence 428835 from Patent EP1572962.
JD085597 - Sequence 66621 from Patent EP1572962.
JD301614 - Sequence 282638 from Patent EP1572962.
JD102593 - Sequence 83617 from Patent EP1572962.
JD432344 - Sequence 413368 from Patent EP1572962.
JD560784 - Sequence 541808 from Patent EP1572962.
JD460419 - Sequence 441443 from Patent EP1572962.
JD255633 - Sequence 236657 from Patent EP1572962.

-  Biochemical and Signaling Pathways
  KEGG - Kyoto Encyclopedia of Genes and Genomes
hsa04080 - Neuroactive ligand-receptor interaction

Reactome (by CSHL, EBI, and GO)

Protein P49286 (Reactome details) participates in the following event(s):

R-HSA-419334 Melatonin receptors can bind melatonin
R-HSA-749454 The Ligand:GPCR:Gi complex dissociates
R-HSA-749456 Liganded Gi-activating GPCRs bind inactive heterotrimeric G-protein Gi
R-HSA-380073 Liganded Gi-activating GPCR acts as a GEF for Gi
R-HSA-373076 Class A/1 (Rhodopsin-like receptors)
R-HSA-418594 G alpha (i) signalling events
R-HSA-500792 GPCR ligand binding
R-HSA-388396 GPCR downstream signalling
R-HSA-372790 Signaling by GPCR
R-HSA-162582 Signal Transduction

-  Other Names for This Gene
  Alternate Gene Symbols: ENST00000257068.1, ENST00000257068.2, MTR1B_HUMAN, NM_005959, P49286, uc001pdk.1, uc001pdk.2, uc001pdk.3
UCSC ID: ENST00000257068.3
RefSeq Accession: NM_005959
Protein: P49286 (aka MTR1B_HUMAN or ML1B_HUMAN)
CCDS: CCDS8290.1

-  Methods, Credits, and Use Restrictions
  Click here for details on how this gene model was made and data restrictions if any.