Human Gene CDCA3 (ENST00000538862.7) from GENCODE V44
  Description: Homo sapiens cell division cycle associated 3 (CDCA3), transcript variant 7, non-coding RNA. (from RefSeq NR_138531)
Gencode Transcript: ENST00000538862.7
Gencode Gene: ENSG00000111665.12
Transcript (Including UTRs)
   Position: hg38 chr12:6,848,828-6,851,286 Size: 2,459 Total Exon Count: 6 Strand: -
Coding Region
   Position: hg38 chr12:6,849,043-6,850,952 Size: 1,910 Coding Exon Count: 5 

Page IndexSequence and LinksUniProtKB CommentsPrimersCTDRNA-Seq Expression
Microarray ExpressionRNA StructureProtein StructureOther SpeciesGO AnnotationsmRNA Descriptions
Other NamesMethods
Data last updated at UCSC: 2023-08-18 00:09:47

-  Sequence and Links to Tools and Databases
 
Genomic Sequence (chr12:6,848,828-6,851,286)mRNA (may differ from genome)Protein (268 aa)
Gene SorterGenome BrowserOther Species FASTAGene interactionsTable SchemaAlphaFold
BioGPSEnsemblEntrez GeneExonPrimerGencodeGeneCards
HGNCLynxMalacardsMGIneXtProtOMIM
PubMedUniProtKBWikipediaBioGrid CRISPR DB

-  Comments and Description Text from UniProtKB
  ID: CDCA3_HUMAN
DESCRIPTION: RecName: Full=Cell division cycle-associated protein 3; AltName: Full=Gene-rich cluster protein C8; AltName: Full=Trigger of mitotic entry protein 1; Short=TOME-1;
FUNCTION: F-box-like protein which is required for entry into mitosis. Acts by participating in E3 ligase complexes that mediate the ubiquitination and degradation of WEE1 kinase at G2/M phase (By similarity).
PATHWAY: Protein modification; protein ubiquitination.
SUBUNIT: Interacts with SKP1. Part of a SCF (SKP1-cullin-F-box) protein ligase complex (By similarity).
SUBCELLULAR LOCATION: Cytoplasm, cytosol (By similarity).
DOMAIN: The KEN box is required for the association with the APC/C-Cdh1 complex (By similarity).
PTM: Ubiquitinated and degraded by the APC/C-Cdh1 complex (By similarity).
PTM: Phosphorylated upon DNA damage, probably by ATM or ATR.

-  Primer design for this transcript
 

Primer3Plus can design qPCR Primers that straddle exon-exon-junctions, which amplify only cDNA, not genomic DNA.
Click here to load the transcript sequence and exon structure into Primer3Plus

Exonprimer can design one pair of Sanger sequencing primers around every exon, located in non-genic sequence.
Click here to open Exonprimer with this transcript

To design primers for a non-coding sequence, zoom to a region of interest and select from the drop-down menu: View > In External Tools > Primer3


-  Comparative Toxicogenomics Database (CTD)
  The following chemicals interact with this gene           more ... click here to view the complete list

-  RNA-Seq Expression Data from GTEx (53 Tissues, 570 Donors)
  Highest median expression: 16.14 RPKM in Testis
Total median expression: 57.33 RPKM



View in GTEx track of Genome Browser    View at GTEx portal     View GTEx Body Map

+  Microarray Expression Data
  Press "+" in the title bar above to open this section.

-  mRNA Secondary Structure of 3' and 5' UTRs
 
RegionFold EnergyBasesEnergy/Base
Display As
5' UTR -43.20122-0.354 Picture PostScript Text
3' UTR -57.80215-0.269 Picture PostScript Text

The RNAfold program from the Vienna RNA Package is used to perform the secondary structure predictions and folding calculations. The estimated folding energy is in kcal/mol. The more negative the energy, the more secondary structure the RNA is likely to have.

-  Protein Domain and Structure Information
  ModBase Predicted Comparative 3D Structure on Q99618
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The pictures above may be empty if there is no ModBase structure for the protein. The ModBase structure frequently covers just a fragment of the protein. You may be asked to log onto ModBase the first time you click on the pictures. It is simplest after logging in to just click on the picture again to get to the specific info on that model.

-  Orthologous Genes in Other Species
  Orthologies between human, mouse, and rat are computed by taking the best BLASTP hit, and filtering out non-syntenic hits. For more distant species reciprocal-best BLASTP hits are used. Note that the absence of an ortholog in the table below may reflect incomplete annotations in the other species rather than a true absence of the orthologous gene.
MouseRatZebrafishD. melanogasterC. elegansS. cerevisiae
Genome BrowserGenome BrowserNo orthologNo orthologNo orthologNo ortholog
Gene Details     
Gene Sorter     
MGIRGD    
Protein SequenceProtein Sequence    
AlignmentAlignment    

-  Gene Ontology (GO) Annotations with Structured Vocabulary
  Molecular Function:
GO:0005515 protein binding

Biological Process:
GO:0007049 cell cycle
GO:0008150 biological_process
GO:0016567 protein ubiquitination
GO:0051301 cell division

Cellular Component:
GO:0005737 cytoplasm
GO:0005829 cytosol
GO:0005913 cell-cell adherens junction


-  Descriptions from all associated GenBank mRNAs
  AK092246 - Homo sapiens cDNA FLJ34927 fis, clone NT2RP7003511.
AX747429 - Sequence 954 from Patent EP1308459.
AK291421 - Homo sapiens cDNA FLJ78107 complete cds, highly similar to Homo sapiens cell division cycle associated 3 (CDCA3), mRNA.
BC002551 - Homo sapiens cell division cycle associated 3, mRNA (cDNA clone MGC:2577 IMAGE:3138951), complete cds.
JD073200 - Sequence 54224 from Patent EP1572962.
JD521316 - Sequence 502340 from Patent EP1572962.
JD382931 - Sequence 363955 from Patent EP1572962.
AK312843 - Homo sapiens cDNA, FLJ93285, highly similar to Homo sapiens cell division cycle associated 3 (CDCA3), mRNA.
KJ894876 - Synthetic construct Homo sapiens clone ccsbBroadEn_04270 CDCA3 gene, encodes complete protein.
JD123323 - Sequence 104347 from Patent EP1572962.
JD395838 - Sequence 376862 from Patent EP1572962.

-  Other Names for This Gene
  Alternate Gene Symbols: A8K5V6, C8, CDCA3_HUMAN, D3DUS6, ENST00000538862.1, ENST00000538862.2, ENST00000538862.3, ENST00000538862.4, ENST00000538862.5, ENST00000538862.6, GRCC8, NR_138531, Q99618, TOME1, uc001qrg.1, uc001qrg.2, uc001qrg.3, uc001qrg.4
UCSC ID: ENST00000538862.7
RefSeq Accession: NM_031299
Protein: Q99618 (aka CDCA3_HUMAN)
CCDS: CCDS8565.1

-  Methods, Credits, and Use Restrictions
  Click here for details on how this gene model was made and data restrictions if any.