Human Gene USP5 (ENST00000229268.13) from GENCODE V44
Description: Homo sapiens ubiquitin specific peptidase 5 (USP5), transcript variant 12, non-coding RNA. (from RefSeq NR_168455) RefSeq Summary (NM_001098536): Ubiquitin (see MIM 191339)-dependent proteolysis is a complex pathway of protein metabolism implicated in such diverse cellular functions as maintenance of chromatin structure, receptor function, and degradation of abnormal proteins. A late step of the process involves disassembly of the polyubiquitin chains on degraded proteins into ubiquitin monomers. USP5 disassembles branched polyubiquitin chains by a sequential exo mechanism, starting at the proximal end of the chain (Wilkinson et al., 1995 [PubMed 7578059]).[supplied by OMIM, Mar 2010]. Gencode Transcript: ENST00000229268.13 Gencode Gene: ENSG00000111667.14 Transcript (Including UTRs) Position: hg38 chr12:6,852,150-6,866,632 Size: 14,483 Total Exon Count: 20 Strand: + Coding Region Position: hg38 chr12:6,852,180-6,866,077 Size: 13,898 Coding Exon Count: 20
ID:UBP5_HUMAN DESCRIPTION: RecName: Full=Ubiquitin carboxyl-terminal hydrolase 5; EC=3.4.19.12; AltName: Full=Deubiquitinating enzyme 5; AltName: Full=Isopeptidase T; AltName: Full=Ubiquitin thioesterase 5; AltName: Full=Ubiquitin-specific-processing protease 5; FUNCTION: Cleaves linear and branched multiubiquitin polymers with a marked preference for branched polymers. Involved in unanchored 'Lys-48'-linked polyubiquitin disassembly. Binds linear and 'Lys- 63'-linked polyubiquitin with a lower affinity. Knock-down of USP5 causes the accumulation of p53/TP53 and an increase in p53/TP53 transcriptional activity because the unanchored polyubiquitin that accumulates is able to compete with ubiquitinated p53/TP53 but not with MDM2 for proteasomal recognition. CATALYTIC ACTIVITY: Thiol-dependent hydrolysis of ester, thioester, amide, peptide and isopeptide bonds formed by the C- terminal Gly of ubiquitin (a 76-residue protein attached to proteins as an intracellular targeting signal). COFACTOR: Binds 1 zinc ion. SUBUNIT: Interacts with TRIML1 (By similarity). INTERACTION: O75528:TADA3; NbExp=2; IntAct=EBI-741277, EBI-473249; DOMAIN: The UBP-type zinc finger domain interacts selectively with an unmodified C-terminus of the proximal ubiquitin. Both UBA domains are involved in polyubiquitin recognition. MISCELLANEOUS: The UBP-type zinc finger domain crystallizes as a dimer linked by a disulfide bond between the Cys-195 residues of both molecules, but there is no evidence that the full-length USP5 exists as a dimer. SIMILARITY: Belongs to the peptidase C19 family. SIMILARITY: Contains 2 UBA domains. SIMILARITY: Contains 1 UBP-type zinc finger.
The RNAfold program from the Vienna RNA Package is used to perform the secondary structure predictions and folding calculations. The estimated folding energy is in kcal/mol. The more negative the energy, the more secondary structure the RNA is likely to have.
ModBase Predicted Comparative 3D Structure on P45974
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Orthologous Genes in Other Species
Orthologies between human, mouse, and rat are computed by taking the best BLASTP hit, and filtering out non-syntenic hits. For more distant species reciprocal-best BLASTP hits are used. Note that the absence of an ortholog in the table below may reflect incomplete annotations in the other species rather than a true absence of the orthologous gene.
Gene Ontology (GO) Annotations with Structured Vocabulary
Molecular Function: GO:0004197 cysteine-type endopeptidase activity GO:0004843 thiol-dependent ubiquitin-specific protease activity GO:0005515 protein binding GO:0008233 peptidase activity GO:0008234 cysteine-type peptidase activity GO:0008270 zinc ion binding GO:0016787 hydrolase activity GO:0036459 thiol-dependent ubiquitinyl hydrolase activity GO:0043130 ubiquitin binding GO:0046872 metal ion binding
Biological Process: GO:0006508 proteolysis GO:0006511 ubiquitin-dependent protein catabolic process GO:0016579 protein deubiquitination GO:0032436 positive regulation of proteasomal ubiquitin-dependent protein catabolic process GO:0071108 protein K48-linked deubiquitination
US Server
