Human Gene SELPLG (ENST00000550948.2) from GENCODE V44
Description: Homo sapiens selectin P ligand (SELPLG), transcript variant 2, mRNA. (from RefSeq NM_003006) RefSeq Summary (NM_003006): This gene encodes a glycoprotein that functions as a high affinity counter-receptor for the cell adhesion molecules P-, E- and L- selectin expressed on myeloid cells and stimulated T lymphocytes. As such, this protein plays a critical role in leukocyte trafficking during inflammation by tethering of leukocytes to activated platelets or endothelia expressing selectins. This protein requires two post-translational modifications, tyrosine sulfation and the addition of the sialyl Lewis x tetrasaccharide (sLex) to its O-linked glycans, for its high-affinity binding activity. Aberrant expression of this gene and polymorphisms in this gene are associated with defects in the innate and adaptive immune response. Alternate splicing results in multiple transcript variants.[provided by RefSeq, Apr 2011]. Gencode Transcript: ENST00000550948.2 Gencode Gene: ENSG00000110876.10 Transcript (Including UTRs) Position: hg38 chr12:108,621,895-108,633,894 Size: 12,000 Total Exon Count: 2 Strand: - Coding Region Position: hg38 chr12:108,623,069-108,624,307 Size: 1,239 Coding Exon Count: 1
ID:SELPL_HUMAN DESCRIPTION: RecName: Full=P-selectin glycoprotein ligand 1; Short=PSGL-1; AltName: Full=Selectin P ligand; AltName: CD_antigen=CD162; Flags: Precursor; FUNCTION: A SLe(x)-type glycan, which through high affinity, calcium-dependent interactions with E-, P- and L-selectins, mediates rapid rolling of leukocytes over vascular surfaces during the initial steps in inflammation. PSGL1 is critical for the initial leukocyte capture. SUBUNIT: Homodimer; disulfide-linked. Interaction with P-, E- and L-selectins, through their lectin/EGF domains, is required for promoting recruitment and rolling of leukocytes. These interactions require sialyl Lewis X glycan modification but there is a differing dependence for tyrosine sulfations. Sulfation on Tyr-51 of PSGL1 is most important for high affinity L- selectin/SELL binding while P-selectin/SELP requires sulfation on Tyr-48. E-selectin/SELE binds with much lower affinity and requires the sLe(x) epitope, but apparantly not tyrosine sulfation. Dimerization appears not to be required for P- selectin/SELP binding. Interacts with SNX20. Interacts with MSN and SYK; mediates the activation of SYK by SELPLG. INTERACTION: Q7Z614:SNX20; NbExp=5; IntAct=EBI-1030190, EBI-744896; SUBCELLULAR LOCATION: Membrane; Single-pass type I membrane protein. TISSUE SPECIFICITY: Expressed on neutrophils, monocytes and most lymphocytes. PTM: Displays complex, core-2, sialylated and fucosylated O-linked oligosaccharides, at least some of which appear to contain poly-N- acetyllactosamine with varying degrees of substitution. Mainly disialylated or neutral forms of the core-2 tetrasaccharide, Galbeta1-->4GlcNAcbeta1-->6(Galbeta1-->3)GalNAcOH. The GlcN:GalN ratio is approximately 2:1 and the Man:Fuc ratio 3:5. Contains about 14% fucose with alpha-1,3 linkage present in two forms: One species is a disialylated, monofucosylated glycan, and the other, a monosialylated, trifucosylated glycan with a polylactosamine backbone. The fucosylated forms carry the Lewis antigen and are important for interaction with selectins and for functioning in leukocyte rolling. The modification containing the sialyl Lewis X glycan is on Thr-57. No sulfated O-glycans. Some N-glycosylation. PTM: Sulfation, in conjunction with the SLe(x)-containing glycan, is necessary for P- and L-selectin binding. High affinity P- selectin binding has a preferred requirement for the isomer sulfated on both Tyr-48 and Tyr-51, whereas L-selectin binding requires predominantly sulfation on Tyr-51 with sulfation on Tyr- 48 playing only a minor role. These sulfations play an important role in L- and P-selectin-mediated neutrophil recruitment, and leukocyte rolling. WEB RESOURCE: Name=Wikipedia; Note=P-selectin glycoprotein ligand 1 entry; URL="http://en.wikipedia.org/wiki/P-selectin_glycoprotein_ligand-1"; WEB RESOURCE: Name=SeattleSNPs; URL="http://pga.gs.washington.edu/data/selplg/";
The RNAfold program from the Vienna RNA Package is used to perform the secondary structure predictions and folding calculations. The estimated folding energy is in kcal/mol. The more negative the energy, the more secondary structure the RNA is likely to have.
ModBase Predicted Comparative 3D Structure on Q14242
Front
Top
Side
The pictures above may be empty if there is no ModBase structure for the protein. The ModBase structure frequently covers just a fragment of the protein. You may be asked to log onto ModBase the first time you click on the pictures. It is simplest after logging in to just click on the picture again to get to the specific info on that model.
Orthologous Genes in Other Species
Orthologies between human, mouse, and rat are computed by taking the best BLASTP hit, and filtering out non-syntenic hits. For more distant species reciprocal-best BLASTP hits are used. Note that the absence of an ortholog in the table below may reflect incomplete annotations in the other species rather than a true absence of the orthologous gene.
AK295238 - Homo sapiens cDNA FLJ50962 complete cds, highly similar to P-selectin glycoprotein ligand 1 precursor. AK098315 - Homo sapiens cDNA FLJ40996 fis, clone UTERU2015880, highly similar to P-SELECTIN GLYCOPROTEIN LIGAND 1 PRECURSOR. AK225644 - Homo sapiens mRNA for P-selectin glycoprotein ligand 1 precursor variant, clone: SPL06709. BC029782 - Homo sapiens selectin P ligand, mRNA (cDNA clone MGC:34401 IMAGE:5180824), complete cds. AK127638 - Homo sapiens cDNA FLJ45736 fis, clone JCMLC2002095, weakly similar to P-selectin glycoprotein ligand 1 precursor. JD495426 - Sequence 476450 from Patent EP1572962. AK298738 - Homo sapiens cDNA FLJ51042 complete cds, highly similar to P-selectin glycoprotein ligand 1 precursor. JD289497 - Sequence 270521 from Patent EP1572962. JD486992 - Sequence 468016 from Patent EP1572962. JD293043 - Sequence 274067 from Patent EP1572962. U02297 - Human P-selectin ligand mRNA, complete cds. JD487078 - Sequence 468102 from Patent EP1572962. JD544820 - Sequence 525844 from Patent EP1572962. JD259666 - Sequence 240690 from Patent EP1572962. AK290395 - Homo sapiens cDNA FLJ78434 complete cds, highly similar to Homo sapiens selectin P ligand (SELPLG), mRNA. JD458141 - Sequence 439165 from Patent EP1572962. JD207014 - Sequence 188038 from Patent EP1572962. AK300057 - Homo sapiens cDNA FLJ52447 complete cds, highly similar to P-selectin glycoprotein ligand 1 precursor. AK298742 - Homo sapiens cDNA FLJ53082 complete cds, highly similar to P-selectin glycoprotein ligand 1 precursor. JD152750 - Sequence 133774 from Patent EP1572962. JD122870 - Sequence 103894 from Patent EP1572962. JD163977 - Sequence 145001 from Patent EP1572962. JD134591 - Sequence 115615 from Patent EP1572962. DQ892117 - Synthetic construct clone IMAGE:100004747; FLH183189.01X; RZPDo839G12140D selectin P ligand (SELPLG) gene, encodes complete protein. KJ892124 - Synthetic construct Homo sapiens clone ccsbBroadEn_01518 SELPLG gene, encodes complete protein. KR710699 - Synthetic construct Homo sapiens clone CCSBHm_00015677 SELPLG (SELPLG) mRNA, encodes complete protein. DQ895313 - Synthetic construct Homo sapiens clone IMAGE:100009773; FLH183185.01L; RZPDo839G12139D selectin P ligand (SELPLG) gene, encodes complete protein. AB464193 - Synthetic construct DNA, clone: pF1KB8995, Homo sapiens SELPLG gene for selectin P ligand, without stop codon, in Flexi system. CU688740 - Synthetic construct Homo sapiens gateway clone IMAGE:100019245 5' read SELPLG mRNA. JD056782 - Sequence 37806 from Patent EP1572962.
Biochemical and Signaling Pathways
KEGG - Kyoto Encyclopedia of Genes and Genomes hsa04514 - Cell adhesion molecules (CAMs)
BioCarta from NCI Cancer Genome Anatomy Project h_LairPathway - Cells and Molecules involved in local acute inflammatory response h_granulocytesPathway - Adhesion and Diapedesis of Granulocytes
Reactome (by CSHL, EBI, and GO)
Protein Q14242 (Reactome details) participates in the following event(s):
US Server
