Human Gene WDR25 (ENST00000335290.10) from GENCODE V44
  Description: Homo sapiens WD repeat domain 25 (WDR25), transcript variant 1, mRNA. (from RefSeq NM_024515)
RefSeq Summary (NM_024515): This gene encodes a protein containing 7 WD repeats. WD repeats are approximately 30 to 40-amino acid domains containing several conserved residues, typically having a Tryptophan-Aspartic acid dipeptide (WD) at the C-terminal end. WD domains are involved in protein-protein interactions in a variety of cellular processes, including cell cycle progression, signal transduction, apoptosis, and gene regulation. Alternative splicing results in multiple transcript variants. [provided by RefSeq, Apr 2017].
Gencode Transcript: ENST00000335290.10
Gencode Gene: ENSG00000176473.14
Transcript (Including UTRs)
   Position: hg38 chr14:100,376,494-100,530,303 Size: 153,810 Total Exon Count: 7 Strand: +
Coding Region
   Position: hg38 chr14:100,380,925-100,530,041 Size: 149,117 Coding Exon Count: 6 

Page IndexSequence and LinksUniProtKB CommentsPrimersCTDRNA-Seq Expression
Microarray ExpressionRNA StructureProtein StructureOther SpeciesGO AnnotationsmRNA Descriptions
Other NamesMethods
Data last updated at UCSC: 2023-08-18 00:09:47

-  Sequence and Links to Tools and Databases
 
Genomic Sequence (chr14:100,376,494-100,530,303)mRNA (may differ from genome)Protein (544 aa)
Gene SorterGenome BrowserOther Species FASTAGene interactionsTable SchemaAlphaFold
BioGPSEnsemblEntrez GeneExonPrimerGencodeGeneCards
HGNCHPRDLynxMalacardsMGIneXtProt
OMIMPubMedUniProtKBBioGrid CRISPR DB

-  Comments and Description Text from UniProtKB
  ID: WDR25_HUMAN
DESCRIPTION: RecName: Full=WD repeat-containing protein 25;
TISSUE SPECIFICITY: Expressed in heart, muscle, testis, ovary, uterus and prostate.
SIMILARITY: Contains 7 WD repeats.

-  Primer design for this transcript
 

Primer3Plus can design qPCR Primers that straddle exon-exon-junctions, which amplify only cDNA, not genomic DNA.
Click here to load the transcript sequence and exon structure into Primer3Plus

Exonprimer can design one pair of Sanger sequencing primers around every exon, located in non-genic sequence.
Click here to open Exonprimer with this transcript

To design primers for a non-coding sequence, zoom to a region of interest and select from the drop-down menu: View > In External Tools > Primer3


-  Comparative Toxicogenomics Database (CTD)
  The following chemicals interact with this gene

-  RNA-Seq Expression Data from GTEx (53 Tissues, 570 Donors)
  Highest median expression: 4.46 RPKM in Pituitary
Total median expression: 124.61 RPKM



View in GTEx track of Genome Browser    View at GTEx portal     View GTEx Body Map

+  Microarray Expression Data
  Press "+" in the title bar above to open this section.

-  mRNA Secondary Structure of 3' and 5' UTRs
 
RegionFold EnergyBasesEnergy/Base
Display As
5' UTR -73.50226-0.325 Picture PostScript Text
3' UTR -87.90262-0.335 Picture PostScript Text

The RNAfold program from the Vienna RNA Package is used to perform the secondary structure predictions and folding calculations. The estimated folding energy is in kcal/mol. The more negative the energy, the more secondary structure the RNA is likely to have.

-  Protein Domain and Structure Information
  InterPro Domains: Graphical view of domain structure
IPR015943 - WD40/YVTN_repeat-like_dom
IPR001680 - WD40_repeat
IPR017986 - WD40_repeat_dom

Pfam Domains:
PF00400 - WD domain, G-beta repeat

ModBase Predicted Comparative 3D Structure on Q64LD2
FrontTopSide
The pictures above may be empty if there is no ModBase structure for the protein. The ModBase structure frequently covers just a fragment of the protein. You may be asked to log onto ModBase the first time you click on the pictures. It is simplest after logging in to just click on the picture again to get to the specific info on that model.

-  Orthologous Genes in Other Species
  Orthologies between human, mouse, and rat are computed by taking the best BLASTP hit, and filtering out non-syntenic hits. For more distant species reciprocal-best BLASTP hits are used. Note that the absence of an ortholog in the table below may reflect incomplete annotations in the other species rather than a true absence of the orthologous gene.
MouseRatZebrafishD. melanogasterC. elegansS. cerevisiae
Genome BrowserGenome BrowserNo orthologNo orthologNo orthologNo ortholog
Gene Details     
Gene Sorter     
MGIRGD    
Protein SequenceProtein Sequence    
AlignmentAlignment    

-  Gene Ontology (GO) Annotations with Structured Vocabulary
  Molecular Function:
GO:0005515 protein binding


-  Descriptions from all associated GenBank mRNAs
  AK291960 - Homo sapiens cDNA FLJ76429 complete cds, highly similar to Homo sapiens WD repeat domain 25 (WDR25), mRNA.
GQ891526 - Homo sapiens clone HEL-S-247 epididymis secretory sperm binding protein mRNA, complete cds.
BC067872 - Homo sapiens WD repeat domain 25, mRNA (cDNA clone IMAGE:30338475).
AY295082 - Homo sapiens pre-mRNA splicing factor-like protein mRNA, complete cds.
BC007953 - Homo sapiens WD repeat domain 25, mRNA (cDNA clone IMAGE:4299080).
BC003641 - Homo sapiens WD repeat domain 25, mRNA (cDNA clone MGC:4645 IMAGE:3529568), complete cds.
CU678554 - Synthetic construct Homo sapiens gateway clone IMAGE:100018740 5' read WDR25 mRNA.
KJ903173 - Synthetic construct Homo sapiens clone ccsbBroadEn_12567 WDR25 gene, encodes complete protein.
BX248767 - human full-length cDNA 5-PRIME end of clone CS0DD009YE24 of Neuroblastoma of Homo sapiens (human).
JD119512 - Sequence 100536 from Patent EP1572962.
JD328261 - Sequence 309285 from Patent EP1572962.
JD177606 - Sequence 158630 from Patent EP1572962.
JD438594 - Sequence 419618 from Patent EP1572962.
JD435115 - Sequence 416139 from Patent EP1572962.
JD297826 - Sequence 278850 from Patent EP1572962.

-  Other Names for This Gene
  Alternate Gene Symbols: A8K7E5, ENST00000335290.1, ENST00000335290.2, ENST00000335290.3, ENST00000335290.4, ENST00000335290.5, ENST00000335290.6, ENST00000335290.7, ENST00000335290.8, ENST00000335290.9, NM_024515, Q64LD2, Q6NVV6, Q86TQ4, Q9BTK5, uc001yhn.1, uc001yhn.2, uc001yhn.3, uc001yhn.4, WDR25_HUMAN
UCSC ID: ENST00000335290.10
RefSeq Accession: NM_024515
Protein: Q64LD2 (aka WDR25_HUMAN)
CCDS: CCDS32157.1

-  Methods, Credits, and Use Restrictions
  Click here for details on how this gene model was made and data restrictions if any.