Human Gene FBXO22 (ENST00000308275.8) from GENCODE V44
  Description: Homo sapiens F-box protein 22 (FBXO22), transcript variant 1, mRNA. (from RefSeq NM_147188)
RefSeq Summary (NM_147188): This gene encodes a member of the F-box protein family which is characterized by an approximately 40 amino acid motif, the F-box. The F-box proteins constitute one of the four subunits of the ubiquitin protein ligase complex called SCFs (SKP1-cullin-F-box), which function in phosphorylation-dependent ubiquitination. The F-box proteins are divided into 3 classes: Fbws containing WD-40 domains, Fbls containing leucine-rich repeats, and Fbxs containing either different protein-protein interaction modules or no recognizable motifs. The protein encoded by this gene belongs to the Fbxs class and, as a transcriptional target of the tumor protein p53, is thought to be involved in degradation of specific proteins in response to p53 induction. Alternative splicing results in multiple transcript variants. [provided by RefSeq, Dec 2010].
Gencode Transcript: ENST00000308275.8
Gencode Gene: ENSG00000167196.14
Transcript (Including UTRs)
   Position: hg38 chr15:75,903,878-75,942,511 Size: 38,634 Total Exon Count: 7 Strand: +
Coding Region
   Position: hg38 chr15:75,903,964-75,933,102 Size: 29,139 Coding Exon Count: 7 

Page IndexSequence and LinksUniProtKB CommentsPrimersCTDRNA-Seq Expression
Microarray ExpressionRNA StructureProtein StructureOther SpeciesGO AnnotationsmRNA Descriptions
PathwaysOther NamesMethods
Data last updated at UCSC: 2023-08-18 00:09:47

-  Sequence and Links to Tools and Databases
Genomic Sequence (chr15:75,903,878-75,942,511)mRNA (may differ from genome)Protein (403 aa)
Gene SorterGenome BrowserOther Species FASTAGene interactionsTable SchemaAlphaFold
BioGPSEnsemblEntrez GeneExonPrimerGencodeGeneCards
PubMedReactomeUniProtKBBioGrid CRISPR DB

-  Comments and Description Text from UniProtKB
DESCRIPTION: RecName: Full=F-box only protein 22; AltName: Full=F-box protein FBX22p44;
FUNCTION: Substrate-recognition component of the SCF (SKP1-CUL1-F- box protein)-type E3 ubiquitin ligase complex (By similarity).
SUBUNIT: Directly interacts with SKP1 and CUL1 (By similarity).
TISSUE SPECIFICITY: Predominantly expressed in liver.
SIMILARITY: Contains 1 F-box domain.
SEQUENCE CAUTION: Sequence=AAH39024.1; Type=Erroneous translation; Note=Wrong choice of CDS;

-  Primer design for this transcript

Primer3Plus can design qPCR Primers that straddle exon-exon-junctions, which amplify only cDNA, not genomic DNA.
Click here to load the transcript sequence and exon structure into Primer3Plus

Exonprimer can design one pair of Sanger sequencing primers around every exon, located in non-genic sequence.
Click here to open Exonprimer with this transcript

To design primers for a non-coding sequence, zoom to a region of interest and select from the drop-down menu: View > In External Tools > Primer3

-  Comparative Toxicogenomics Database (CTD)
  The following chemicals interact with this gene           more ... click here to view the complete list

-  RNA-Seq Expression Data from GTEx (53 Tissues, 570 Donors)
  Highest median expression: 10.35 RPKM in Cells - EBV-transformed lymphocytes
Total median expression: 185.51 RPKM

View in GTEx track of Genome Browser    View at GTEx portal     View GTEx Body Map

+  Microarray Expression Data
  Press "+" in the title bar above to open this section.

-  mRNA Secondary Structure of 3' and 5' UTRs
RegionFold EnergyBasesEnergy/Base
Display As
5' UTR -40.1086-0.466 Picture PostScript Text
3' UTR -2512.409409-0.267 Picture PostScript Text

The RNAfold program from the Vienna RNA Package is used to perform the secondary structure predictions and folding calculations. The estimated folding energy is in kcal/mol. The more negative the energy, the more secondary structure the RNA is likely to have.

-  Protein Domain and Structure Information
  InterPro Domains: Graphical view of domain structure
IPR001810 - F-box_dom_cyclin-like
IPR019494 - FIST_C_domain

Pfam Domains:
PF00646 - F-box domain
PF10442 - FIST C domain

ModBase Predicted Comparative 3D Structure on Q8NEZ5
The pictures above may be empty if there is no ModBase structure for the protein. The ModBase structure frequently covers just a fragment of the protein. You may be asked to log onto ModBase the first time you click on the pictures. It is simplest after logging in to just click on the picture again to get to the specific info on that model.

-  Orthologous Genes in Other Species
  Orthologies between human, mouse, and rat are computed by taking the best BLASTP hit, and filtering out non-syntenic hits. For more distant species reciprocal-best BLASTP hits are used. Note that the absence of an ortholog in the table below may reflect incomplete annotations in the other species rather than a true absence of the orthologous gene.
MouseRatZebrafishD. melanogasterC. elegansS. cerevisiae
Genome BrowserGenome BrowserGenome BrowserNo orthologNo orthologNo ortholog
Gene Details     
Gene Sorter     
Protein SequenceProtein SequenceProtein Sequence   

-  Gene Ontology (GO) Annotations with Structured Vocabulary
  Molecular Function:
GO:0004842 ubiquitin-protein transferase activity
GO:0005515 protein binding

Biological Process:
GO:0000209 protein polyubiquitination
GO:0006464 cellular protein modification process
GO:0006511 ubiquitin-dependent protein catabolic process
GO:0006913 nucleocytoplasmic transport
GO:0009267 cellular response to starvation
GO:0010830 regulation of myotube differentiation
GO:0032436 positive regulation of proteasomal ubiquitin-dependent protein catabolic process
GO:0043687 post-translational protein modification
GO:0048742 regulation of skeletal muscle fiber development
GO:2000060 positive regulation of protein ubiquitination involved in ubiquitin-dependent protein catabolic process

Cellular Component:
GO:0005634 nucleus
GO:0005737 cytoplasm
GO:0005829 cytosol
GO:0030018 Z disc

-  Descriptions from all associated GenBank mRNAs
  LF384551 - JP 2014500723-A/192054: Polycomb-Associated Non-Coding RNAs.
MA620128 - JP 2018138019-A/192054: Polycomb-Associated Non-Coding RNAs.
BC020204 - Homo sapiens F-box protein 22, mRNA (cDNA clone MGC:31799 IMAGE:4546640), complete cds.
AK307084 - Homo sapiens cDNA, FLJ97032.
AK024048 - Homo sapiens cDNA FLJ13986 fis, clone Y79AA1001923, weakly similar to Homo sapiens F-box protein Fbx22 (FBX22) gene.
BC041691 - Homo sapiens F-box protein 22, mRNA (cDNA clone MGC:48729 IMAGE:5246992), complete cds.
AK223500 - Homo sapiens mRNA for F-box only protein 22 isoform a variant, clone: FCC121B10.
BC039024 - Homo sapiens F-box protein 22, mRNA (cDNA clone MGC:47575 IMAGE:6071245), complete cds.
AK294311 - Homo sapiens cDNA FLJ50132 complete cds, highly similar to F-box only protein 22.
BC008762 - Homo sapiens F-box protein 22, mRNA (cDNA clone IMAGE:3349806), **** WARNING: chimeric clone ****.
BC032540 - Homo sapiens F-box protein 22, mRNA (cDNA clone MGC:44940 IMAGE:5529506), complete cds.
CU692024 - Synthetic construct Homo sapiens gateway clone IMAGE:100021186 5' read FBXO22 mRNA.
HQ448526 - Synthetic construct Homo sapiens clone IMAGE:100071961; CCSB011319_01 F-box protein 22 (FBXO22) gene, encodes complete protein.
KJ898615 - Synthetic construct Homo sapiens clone ccsbBroadEn_08009 FBXO22 gene, encodes complete protein.
AY005144 - Homo sapiens F-box protein FBX22p44 mRNA, complete cds.
AK026282 - Homo sapiens cDNA: FLJ22629 fis, clone HSI06179.
JD280000 - Sequence 261024 from Patent EP1572962.
JD229154 - Sequence 210178 from Patent EP1572962.
JD173817 - Sequence 154841 from Patent EP1572962.
JD369085 - Sequence 350109 from Patent EP1572962.
JD492572 - Sequence 473596 from Patent EP1572962.
JD337346 - Sequence 318370 from Patent EP1572962.
JD315609 - Sequence 296633 from Patent EP1572962.
JD281815 - Sequence 262839 from Patent EP1572962.
JD521401 - Sequence 502425 from Patent EP1572962.
JD088564 - Sequence 69588 from Patent EP1572962.
JD130539 - Sequence 111563 from Patent EP1572962.
JD144249 - Sequence 125273 from Patent EP1572962.
AL360145 - Homo sapiens mRNA full length insert cDNA clone EUROIMAGE 839551.
JD369144 - Sequence 350168 from Patent EP1572962.

-  Biochemical and Signaling Pathways
  Reactome (by CSHL, EBI, and GO)

Protein Q8NEZ5 (Reactome details) participates in the following event(s):

R-HSA-8952620 NEDD8:AcM-UBE2M binds CRL1 E3 ubiquitin ligase complex
R-HSA-8956200 MyrG-DCUN1D3 binds CRL1 E3 ubiquitin ligase complex
R-HSA-983157 Interaction of E3 with substrate and E2-Ub complex
R-HSA-983147 Release of E3 from polyubiquitinated substrate
R-HSA-8955241 CAND1 binds cytosolic CRL E3 ubiquitin ligases
R-HSA-8952618 AcM-UBE2M transfers NEDD8 to CRL1 E3 ubiquitin ligase complex
R-HSA-8955289 COMMDs displace CAND1 from cytosolic CRL E3 ubiquitin ligase complexes
R-HSA-8956040 COP9 signalosome deneddylates cytosolic CRL E3 ubiquitin ligase complexes
R-HSA-983140 Transfer of Ub from E2 to substrate and release of E2
R-HSA-8951664 Neddylation
R-HSA-983168 Antigen processing: Ubiquitination & Proteasome degradation
R-HSA-597592 Post-translational protein modification
R-HSA-983169 Class I MHC mediated antigen processing & presentation
R-HSA-392499 Metabolism of proteins
R-HSA-1280218 Adaptive Immune System
R-HSA-168256 Immune System

-  Other Names for This Gene
  Alternate Gene Symbols: ENST00000308275.1, ENST00000308275.2, ENST00000308275.3, ENST00000308275.4, ENST00000308275.5, ENST00000308275.6, ENST00000308275.7, FBX22, FBX22_HUMAN, NM_147188, Q0D2P8, Q6PIL5, Q8IXW3, Q8NEZ5, Q9H824, Q9UKC0, uc002bbk.1, uc002bbk.2, uc002bbk.3, uc002bbk.4, uc002bbk.5
UCSC ID: ENST00000308275.8
RefSeq Accession: NM_147188
Protein: Q8NEZ5 (aka FBX22_HUMAN or FX22_HUMAN)
CCDS: CCDS10287.1

-  Methods, Credits, and Use Restrictions
  Click here for details on how this gene model was made and data restrictions if any.