Human Gene MPG (ENST00000356432.8) from GENCODE V44
  Description: Homo sapiens N-methylpurine DNA glycosylase (MPG), transcript variant 2, mRNA. (from RefSeq NM_001015052)
Gencode Transcript: ENST00000356432.8
Gencode Gene: ENSG00000103152.12
Transcript (Including UTRs)
   Position: hg38 chr16:78,225-85,846 Size: 7,622 Total Exon Count: 4 Strand: +
Coding Region
   Position: hg38 chr16:78,310-85,777 Size: 7,468 Coding Exon Count: 4 

Page IndexSequence and LinksUniProtKB CommentsPrimersCTDRNA-Seq Expression
Microarray ExpressionRNA StructureProtein StructureOther SpeciesGO AnnotationsmRNA Descriptions
PathwaysOther NamesMethods
Data last updated at UCSC: 2023-08-18 00:09:47

-  Sequence and Links to Tools and Databases
 
Genomic Sequence (chr16:78,225-85,846)mRNA (may differ from genome)Protein (293 aa)
Gene SorterGenome BrowserOther Species FASTAGene interactionsTable SchemaAlphaFold
BioGPSEnsemblEntrez GeneExonPrimerGencodeGeneCards
HGNCLynxMalacardsMGIneXtProtOMIM
PubMedReactomeUniProtKBWikipediaBioGrid CRISPR DB

-  Comments and Description Text from UniProtKB
  ID: 3MG_HUMAN
DESCRIPTION: RecName: Full=DNA-3-methyladenine glycosylase; EC=3.2.2.21; AltName: Full=3-alkyladenine DNA glycosylase; AltName: Full=3-methyladenine DNA glycosidase; AltName: Full=ADPG; AltName: Full=N-methylpurine-DNA glycosylase;
FUNCTION: Hydrolysis of the deoxyribose N-glycosidic bond to excise 3-methyladenine, and 7-methylguanine from the damaged DNA polymer formed by alkylation lesions.
CATALYTIC ACTIVITY: Hydrolysis of alkylated DNA, releasing 3- methyladenine, 3-methylguanine, 7-methylguanine and 7- methyladenine.
SUBUNIT: Binds MBD1.
INTERACTION: P04156:PRNP; NbExp=4; IntAct=EBI-1043398, EBI-977302;
SUBCELLULAR LOCATION: Nucleus (Potential).
SIMILARITY: Belongs to the DNA glycosylase MPG family.
WEB RESOURCE: Name=NIEHS-SNPs; URL="http://egp.gs.washington.edu/data/mpg/";

-  Primer design for this transcript
 

Primer3Plus can design qPCR Primers that straddle exon-exon-junctions, which amplify only cDNA, not genomic DNA.
Click here to load the transcript sequence and exon structure into Primer3Plus

Exonprimer can design one pair of Sanger sequencing primers around every exon, located in non-genic sequence.
Click here to open Exonprimer with this transcript

To design primers for a non-coding sequence, zoom to a region of interest and select from the drop-down menu: View > In External Tools > Primer3


-  Comparative Toxicogenomics Database (CTD)
  The following chemicals interact with this gene           more ... click here to view the complete list

-  RNA-Seq Expression Data from GTEx (53 Tissues, 570 Donors)
  Highest median expression: 28.79 RPKM in Cells - Cultured fibroblasts
Total median expression: 763.96 RPKM



View in GTEx track of Genome Browser    View at GTEx portal     View GTEx Body Map

+  Microarray Expression Data
  Press "+" in the title bar above to open this section.

-  mRNA Secondary Structure of 3' and 5' UTRs
 
RegionFold EnergyBasesEnergy/Base
Display As
5' UTR -40.7085-0.479 Picture PostScript Text
3' UTR -6.3069-0.091 Picture PostScript Text

The RNAfold program from the Vienna RNA Package is used to perform the secondary structure predictions and folding calculations. The estimated folding energy is in kcal/mol. The more negative the energy, the more secondary structure the RNA is likely to have.

-  Protein Domain and Structure Information
  InterPro Domains: Graphical view of domain structure
IPR011034 - Formyl_transferase_C-like
IPR003180 - PurDNA_glycsylse

Pfam Domains:
PF02245 - Methylpurine-DNA glycosylase (MPG)

Protein Data Bank (PDB) 3-D Structure
MuPIT help
1BNK - X-ray MuPIT 1EWN - X-ray MuPIT 1F4R - X-ray MuPIT 1F6O - X-ray MuPIT 3QI5 - X-ray MuPIT 3UBY - X-ray MuPIT


ModBase Predicted Comparative 3D Structure on P29372
FrontTopSide
The pictures above may be empty if there is no ModBase structure for the protein. The ModBase structure frequently covers just a fragment of the protein. You may be asked to log onto ModBase the first time you click on the pictures. It is simplest after logging in to just click on the picture again to get to the specific info on that model.

-  Orthologous Genes in Other Species
  Orthologies between human, mouse, and rat are computed by taking the best BLASTP hit, and filtering out non-syntenic hits. For more distant species reciprocal-best BLASTP hits are used. Note that the absence of an ortholog in the table below may reflect incomplete annotations in the other species rather than a true absence of the orthologous gene.
MouseRatZebrafishD. melanogasterC. elegansS. cerevisiae
Genome BrowserGenome BrowserNo orthologNo orthologNo orthologNo ortholog
Gene Details     
Gene Sorter     
MGIRGD    
Protein SequenceProtein Sequence    
AlignmentAlignment    

-  Gene Ontology (GO) Annotations with Structured Vocabulary
  Molecular Function:
GO:0003677 DNA binding
GO:0003684 damaged DNA binding
GO:0003824 catalytic activity
GO:0003905 alkylbase DNA N-glycosylase activity
GO:0005515 protein binding
GO:0008725 DNA-3-methyladenine glycosylase activity
GO:0016787 hydrolase activity
GO:0019104 DNA N-glycosylase activity
GO:0043916 DNA-7-methylguanine glycosylase activity
GO:0052821 DNA-7-methyladenine glycosylase activity
GO:0052822 DNA-3-methylguanine glycosylase activity

Biological Process:
GO:0006281 DNA repair
GO:0006284 base-excision repair
GO:0006307 DNA dealkylation involved in DNA repair
GO:0006974 cellular response to DNA damage stimulus
GO:0045007 depurination

Cellular Component:
GO:0005634 nucleus
GO:0005654 nucleoplasm
GO:0005737 cytoplasm
GO:0005739 mitochondrion
GO:0042645 mitochondrial nucleoid


-  Descriptions from all associated GenBank mRNAs
  BC014991 - Homo sapiens N-methylpurine-DNA glycosylase, mRNA (cDNA clone MGC:23226 IMAGE:4909112), complete cds.
AK308654 - Homo sapiens cDNA, FLJ98695.
AY305873 - Homo sapiens proliferation-inducing protein 16 (PIG16) mRNA, complete cds.
AY258284 - Homo sapiens proliferation-inducing protein 11 mRNA, complete cds.
L10752 - Homo sapiens 3-methyl-adenine DNA glycosylase mRNA, complete cds.
CU677157 - Synthetic construct Homo sapiens gateway clone IMAGE:100020690 5' read MPG mRNA.
AB463933 - Synthetic construct DNA, clone: pF1KB8217, Homo sapiens MPG gene for N-methylpurine-DNA glycosylase, without stop codon, in Flexi system.
DQ895912 - Synthetic construct Homo sapiens clone IMAGE:100010372; FLH190258.01L; RZPDo839G0465D N-methylpurine-DNA glycosylase (MPG) gene, encodes complete protein.
KJ891637 - Synthetic construct Homo sapiens clone ccsbBroadEn_01031 MPG gene, encodes complete protein.
KR710557 - Synthetic construct Homo sapiens clone CCSBHm_00014045 MPG (MPG) mRNA, encodes complete protein.
M74905 - Human 3-alkyladenine DNA glycosylase (HAAG) mRNA, complete cds.
S51033 - Homo sapiens N-methylpurine-DNA glycosylase mRNA, partial cds.
M71215 - Human alkyl-N-purine-DNA glycosylase mRNA, 3' end.
X56528 - H.sapiens anpg mRNA.
M99626 - Human Mid1 gene, partial cds.

-  Biochemical and Signaling Pathways
  KEGG - Kyoto Encyclopedia of Genes and Genomes
hsa03410 - Base excision repair

Reactome (by CSHL, EBI, and GO)

Protein P29372 (Reactome details) participates in the following event(s):

R-HSA-110238 MPG glycosylase mediated recognition and binding of 3-methyladenine
R-HSA-110239 MPG glycosylase mediated recognition and binding of ethenoadenine
R-HSA-110240 MPG glycosylase mediated recognition and binding of hypoxanthine
R-HSA-110356 Displacement of MPG glycosylase by APEX1 at the AP site
R-HSA-110248 Cleavage of 3-methyladenine by MPG glycosylase
R-HSA-110250 Cleavage of ethenoadenine by MPG glycosylase
R-HSA-110251 Cleavage of hypoxanthine by MPG glycosylase
R-HSA-110330 Recognition and association of DNA glycosylase with site containing an affected purine
R-HSA-110357 Displacement of DNA glycosylase by APEX1
R-HSA-110331 Cleavage of the damaged purine
R-HSA-73927 Depurination
R-HSA-73933 Resolution of Abasic Sites (AP sites)
R-HSA-73929 Base-Excision Repair, AP Site Formation
R-HSA-73884 Base Excision Repair
R-HSA-73894 DNA Repair

-  Other Names for This Gene
  Alternate Gene Symbols: 3MG_HUMAN, AAG, ANPG, ENST00000356432.1, ENST00000356432.2, ENST00000356432.3, ENST00000356432.4, ENST00000356432.5, ENST00000356432.6, ENST00000356432.7, MID1, NM_001015052, P29372, Q13770, Q15275, Q15961, Q96BZ6, Q96S33, Q9NNX5, uc002cfo.1, uc002cfo.2, uc002cfo.3, uc002cfo.4, uc002cfo.5, uc002cfo.6
UCSC ID: ENST00000356432.8
RefSeq Accession: NM_001015052
Protein: P29372 (aka 3MG_HUMAN)
CCDS: CCDS42087.1, CCDS32346.1, CCDS32345.1

-  Methods, Credits, and Use Restrictions
  Click here for details on how this gene model was made and data restrictions if any.