Human Gene MRPL28 (ENST00000199706.13) from GENCODE V44
Description: Homo sapiens mitochondrial ribosomal protein L28 (MRPL28), mRNA; nuclear gene for mitochondrial product. (from RefSeq NM_006428) RefSeq Summary (NM_006428): Mammalian mitochondrial ribosomal proteins are encoded by nuclear genes and help in protein synthesis within the mitochondrion. Mitochondrial ribosomes (mitoribosomes) consist of a small 28S subunit and a large 39S subunit. They have an estimated 75% protein to rRNA composition compared to prokaryotic ribosomes, where this ratio is reversed. Another difference between mammalian mitoribosomes and prokaryotic ribosomes is that the latter contain a 5S rRNA. Among different species, the proteins comprising the mitoribosome differ greatly in sequence, and sometimes in biochemical properties, which prevents easy recognition by sequence homology. This gene encodes a 39S subunit protein, a part of which was originally isolated by its ability to recognize tyrosinase in an HLA-A24-restricted fashion. [provided by RefSeq, Jul 2008]. Gencode Transcript: ENST00000199706.13 Gencode Gene: ENSG00000086504.18 Transcript (Including UTRs) Position: hg38 chr16:366,969-370,538 Size: 3,570 Total Exon Count: 6 Strand: - Coding Region Position: hg38 chr16:367,675-370,218 Size: 2,544 Coding Exon Count: 5
ID:RM28_HUMAN DESCRIPTION: RecName: Full=39S ribosomal protein L28, mitochondrial; Short=L28mt; Short=MRP-L28; AltName: Full=Melanoma antigen p15; AltName: Full=Melanoma-associated antigen recognized by T-lymphocytes; Flags: Precursor; SUBUNIT: Interacts with OXA1L (By similarity). SUBCELLULAR LOCATION: Mitochondrion. TISSUE SPECIFICITY: Found in a variety of normal tissues including spleen, testes, thymus, liver, kidney, brain, adrenal, lung and retinal tissue. MISCELLANEOUS: Potentially represents an important therapeutic reagent for HLA-A24 patients. This antigen is recognized by tumor- infiltrating lymphocyte (TIL) 1290 in the context of HLA-A24. SIMILARITY: Belongs to the ribosomal protein L28P family. SEQUENCE CAUTION: Sequence=AAC50181.1; Type=Erroneous initiation; Sequence=AAK61226.1; Type=Erroneous gene model prediction;
The RNAfold program from the Vienna RNA Package is used to perform the secondary structure predictions and folding calculations. The estimated folding energy is in kcal/mol. The more negative the energy, the more secondary structure the RNA is likely to have.
ModBase Predicted Comparative 3D Structure on Q13084
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Orthologous Genes in Other Species
Orthologies between human, mouse, and rat are computed by taking the best BLASTP hit, and filtering out non-syntenic hits. For more distant species reciprocal-best BLASTP hits are used. Note that the absence of an ortholog in the table below may reflect incomplete annotations in the other species rather than a true absence of the orthologous gene.
R-HSA-5389839 39S subunit binds 28S subunit:mRNA:fMet-tRNA R-HSA-5419273 Hydrolysis of GTP and dissociation of 28S and 39S subunits R-HSA-5389848 TUFM:GTP:aminoacyl-tRNA binds 55S ribosome:mRNA:fMet-tRNA R-HSA-5389857 Peptide transfer from P-site to A-site (peptide bond formation) R-HSA-5389842 TUFM hydrolyzes GTP and TUFM:GDP dissociates from 55S ribosome R-HSA-5419261 55S ribosome with peptidyl-tRNA in A site binds GFM1:GTP R-HSA-5419281 MRRF binds 55S ribosome:mRNA:tRNA R-HSA-5419279 Translocation of peptidyl-tRNA from A-site to P-site (and translocation of 55S ribosome by 3 bases along mRNA) R-HSA-5419264 MTRF1L (MTRF1a) or ICT1 binds stop codon in 55S ribosome:mRNA:peptidyl-tRNA R-HSA-5419277 GFM2:GTP binds 55S ribosome:mRNA:tRNA:MRRF releasing mRNA and tRNA R-HSA-5368286 Mitochondrial translation initiation R-HSA-5419276 Mitochondrial translation termination R-HSA-5368287 Mitochondrial translation R-HSA-5389840 Mitochondrial translation elongation R-HSA-72766 Translation R-HSA-392499 Metabolism of proteins