Human Gene DNASE1L2 (ENST00000320700.10) from GENCODE V44
  Description: Homo sapiens deoxyribonuclease 1 like 2 (DNASE1L2), transcript variant 1, mRNA. (from RefSeq NM_001374)
Gencode Transcript: ENST00000320700.10
Gencode Gene: ENSG00000167968.13
Transcript (Including UTRs)
   Position: hg38 chr16:2,236,444-2,238,711 Size: 2,268 Total Exon Count: 7 Strand: +
Coding Region
   Position: hg38 chr16:2,236,817-2,238,418 Size: 1,602 Coding Exon Count: 6 

Page IndexSequence and LinksUniProtKB CommentsPrimersCTDRNA-Seq Expression
Microarray ExpressionRNA StructureProtein StructureOther SpeciesGO AnnotationsmRNA Descriptions
Other NamesMethods
Data last updated at UCSC: 2023-08-18 00:09:47

-  Sequence and Links to Tools and Databases
 
Genomic Sequence (chr16:2,236,444-2,238,711)mRNA (may differ from genome)Protein (299 aa)
Gene SorterGenome BrowserOther Species FASTAGene interactionsTable SchemaAlphaFold
BioGPSEnsemblEntrez GeneExonPrimerGencodeGeneCards
HGNCHPRDLynxMalacardsMGIneXtProt
OMIMPubMedUniProtKBWikipediaBioGrid CRISPR DB

-  Comments and Description Text from UniProtKB
  ID: DNSL2_HUMAN
DESCRIPTION: RecName: Full=Deoxyribonuclease-1-like 2; EC=3.1.21.-; AltName: Full=DNase I homolog protein DHP1; AltName: Full=Deoxyribonuclease I-like 2; Short=DNase I-like 2; Flags: Precursor;
INTERACTION: P00519:ABL1; NbExp=1; IntAct=EBI-1751995, EBI-375543; P06241:FYN; NbExp=1; IntAct=EBI-1751995, EBI-515315;
SUBCELLULAR LOCATION: Secreted (Potential).
TISSUE SPECIFICITY: Brain.
SIMILARITY: Belongs to the DNase I family.

-  Primer design for this transcript
 

Primer3Plus can design qPCR Primers that straddle exon-exon-junctions, which amplify only cDNA, not genomic DNA.
Click here to load the transcript sequence and exon structure into Primer3Plus

Exonprimer can design one pair of Sanger sequencing primers around every exon, located in non-genic sequence.
Click here to open Exonprimer with this transcript

To design primers for a non-coding sequence, zoom to a region of interest and select from the drop-down menu: View > In External Tools > Primer3


-  Comparative Toxicogenomics Database (CTD)
  The following chemicals interact with this gene

-  RNA-Seq Expression Data from GTEx (53 Tissues, 570 Donors)
  Highest median expression: 29.76 RPKM in Skin - Sun Exposed (Lower leg)
Total median expression: 158.55 RPKM



View in GTEx track of Genome Browser    View at GTEx portal     View GTEx Body Map

+  Microarray Expression Data
  Press "+" in the title bar above to open this section.

-  mRNA Secondary Structure of 3' and 5' UTRs
 
RegionFold EnergyBasesEnergy/Base
Display As
5' UTR -52.40156-0.336 Picture PostScript Text
3' UTR -147.80293-0.504 Picture PostScript Text

The RNAfold program from the Vienna RNA Package is used to perform the secondary structure predictions and folding calculations. The estimated folding energy is in kcal/mol. The more negative the energy, the more secondary structure the RNA is likely to have.

-  Protein Domain and Structure Information
  InterPro Domains: Graphical view of domain structure
IPR018057 - Deoxyribonuclease-1_AS
IPR016202 - DNase_I-like
IPR008185 - DNase_I_euk
IPR005135 - Endo/exonuclease/phosphatase

Pfam Domains:
PF03372 - Endonuclease/Exonuclease/phosphatase family

ModBase Predicted Comparative 3D Structure on Q92874
FrontTopSide
The pictures above may be empty if there is no ModBase structure for the protein. The ModBase structure frequently covers just a fragment of the protein. You may be asked to log onto ModBase the first time you click on the pictures. It is simplest after logging in to just click on the picture again to get to the specific info on that model.

-  Orthologous Genes in Other Species
  Orthologies between human, mouse, and rat are computed by taking the best BLASTP hit, and filtering out non-syntenic hits. For more distant species reciprocal-best BLASTP hits are used. Note that the absence of an ortholog in the table below may reflect incomplete annotations in the other species rather than a true absence of the orthologous gene.
MouseRatZebrafishD. melanogasterC. elegansS. cerevisiae
Genome BrowserGenome BrowserNo orthologNo orthologNo orthologNo ortholog
Gene Details     
Gene Sorter     
MGIRGD    
Protein SequenceProtein Sequence    
AlignmentAlignment    

-  Gene Ontology (GO) Annotations with Structured Vocabulary
  Molecular Function:
GO:0003677 DNA binding
GO:0004518 nuclease activity
GO:0004519 endonuclease activity
GO:0004536 deoxyribonuclease activity
GO:0005509 calcium ion binding
GO:0016787 hydrolase activity
GO:0046872 metal ion binding

Biological Process:
GO:0001942 hair follicle development
GO:0003335 corneocyte development
GO:0006259 DNA metabolic process
GO:0006308 DNA catabolic process
GO:0090305 nucleic acid phosphodiester bond hydrolysis

Cellular Component:
GO:0005576 extracellular region
GO:0005634 nucleus
GO:0005737 cytoplasm


-  Descriptions from all associated GenBank mRNAs
  KJ891058 - Synthetic construct Homo sapiens clone ccsbBroadEn_00452 DNASE1L2 gene, encodes complete protein.
AK098028 - Homo sapiens cDNA FLJ40709 fis, clone THYMU2027053, highly similar to Deoxyribonuclease I-like 2 precursor (EC 3.1.21.-).
BC063710 - Homo sapiens deoxyribonuclease I-like 2, mRNA (cDNA clone MGC:75492 IMAGE:4800855), complete cds.
BC035205 - Homo sapiens deoxyribonuclease I-like 2, mRNA (cDNA clone MGC:24901 IMAGE:4779847), complete cds.
U62647 - Human DNase 1 homolog (DNAS1L2) mRNA, complete cds.
AY298957 - Homo sapiens deoxyribonuclease I-like 2 short form (DNASE1L2) mRNA, complete cds; alternatively spliced.
JD225599 - Sequence 206623 from Patent EP1572962.
JD338859 - Sequence 319883 from Patent EP1572962.
JD123083 - Sequence 104107 from Patent EP1572962.
JD077264 - Sequence 58288 from Patent EP1572962.

-  Other Names for This Gene
  Alternate Gene Symbols: DHP1, DNAS1L2, DNSL2_HUMAN, ENST00000320700.1, ENST00000320700.2, ENST00000320700.3, ENST00000320700.4, ENST00000320700.5, ENST00000320700.6, ENST00000320700.7, ENST00000320700.8, ENST00000320700.9, NM_001374, Q92874, uc002cpp.1, uc002cpp.2, uc002cpp.3, uc002cpp.4
UCSC ID: ENST00000320700.10
RefSeq Accession: NM_001374
Protein: Q92874 (aka DNSL2_HUMAN)
CCDS: CCDS42105.1

-  Methods, Credits, and Use Restrictions
  Click here for details on how this gene model was made and data restrictions if any.