Human Gene IST1 (ENST00000378799.11) from GENCODE V44
  Description: Homo sapiens IST1 factor associated with ESCRT-III (IST1), transcript variant 1, mRNA. (from RefSeq NM_001270975)
RefSeq Summary (NM_001270975): This gene encodes a protein with MIT-interacting motifs that interacts with components of endosomal sorting complexes required for transport (ESCRT). ESCRT functions in vesicle budding, such as that which occurs during membrane abscission in cytokinesis. There is a pseudogene for this gene on chromosome 19. Alternatively spliced transcript variants encoding multiple isoforms have been observed for this gene. [provided by RefSeq, Aug 2012].
Gencode Transcript: ENST00000378799.11
Gencode Gene: ENSG00000182149.21
Transcript (Including UTRs)
   Position: hg38 chr16:71,895,531-71,931,199 Size: 35,669 Total Exon Count: 10 Strand: +
Coding Region
   Position: hg38 chr16:71,915,641-71,927,813 Size: 12,173 Coding Exon Count: 9 

Page IndexSequence and LinksUniProtKB CommentsPrimersCTDRNA-Seq Expression
Microarray ExpressionRNA StructureProtein StructureOther SpeciesGO AnnotationsmRNA Descriptions
PathwaysOther NamesMethods
Data last updated at UCSC: 2023-08-18 00:09:47

-  Sequence and Links to Tools and Databases
 
Genomic Sequence (chr16:71,895,531-71,931,199)mRNA (may differ from genome)Protein (366 aa)
Gene SorterGenome BrowserOther Species FASTAGene interactionsTable SchemaAlphaFold
BioGPSEnsemblEntrez GeneExonPrimerGencodeGeneCards
HGNCHPRDLynxMalacardsMGIneXtProt
OMIMPubMedReactomeUniProtKBWikipediaBioGrid CRISPR DB

-  Comments and Description Text from UniProtKB
  ID: IST1_HUMAN
DESCRIPTION: RecName: Full=IST1 homolog; Short=hIST1; AltName: Full=Putative MAPK-activating protein PM28;
FUNCTION: Proposed to be involved in specific functions of the ESCRT machinery. Is required for efficient abscission during cytokinesis, but not for HIV-1 budding. The involvement in the MVB pathway is not established. Involved in recruiting VPS4A and/or VPS4B to the midbody of dividing cells.
SUBUNIT: Interacts with CHMP1A, CHMP1B, VPS4A and VTA1. Interacts with SPAST, STAMBP, USP8 and MITD1. May interact with VPS37B. May associate with the ESCRT-I complex.
INTERACTION: P54253:ATXN1; NbExp=2; IntAct=EBI-945994, EBI-930964;
SUBCELLULAR LOCATION: Cytoplasmic vesicle. Note=Localizes to the midbody of dividing cells.
SIMILARITY: Belongs to the IST1 family.
SEQUENCE CAUTION: Sequence=BAA11491.2; Type=Erroneous initiation; Note=Translation N-terminally shortened;

-  Primer design for this transcript
 

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Click here to load the transcript sequence and exon structure into Primer3Plus

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-  Comparative Toxicogenomics Database (CTD)
  The following chemicals interact with this gene

-  RNA-Seq Expression Data from GTEx (53 Tissues, 570 Donors)
  Highest median expression: 57.37 RPKM in Pituitary
Total median expression: 1929.43 RPKM



View in GTEx track of Genome Browser    View at GTEx portal     View GTEx Body Map

+  Microarray Expression Data
  Press "+" in the title bar above to open this section.

-  mRNA Secondary Structure of 3' and 5' UTRs
 
RegionFold EnergyBasesEnergy/Base
Display As
5' UTR -18.4074-0.249 Picture PostScript Text
3' UTR -976.103386-0.288 Picture PostScript Text

The RNAfold program from the Vienna RNA Package is used to perform the secondary structure predictions and folding calculations. The estimated folding energy is in kcal/mol. The more negative the energy, the more secondary structure the RNA is likely to have.

-  Protein Domain and Structure Information
  InterPro Domains: Graphical view of domain structure
IPR005061 - DUF292_euk

Pfam Domains:
PF03398 - Regulator of Vps4 activity in the MVB pathway

Protein Data Bank (PDB) 3-D Structure
MuPIT help
3FRR - X-ray MuPIT 3FRS - X-ray MuPIT


ModBase Predicted Comparative 3D Structure on P53990
FrontTopSide
The pictures above may be empty if there is no ModBase structure for the protein. The ModBase structure frequently covers just a fragment of the protein. You may be asked to log onto ModBase the first time you click on the pictures. It is simplest after logging in to just click on the picture again to get to the specific info on that model.

-  Orthologous Genes in Other Species
  Orthologies between human, mouse, and rat are computed by taking the best BLASTP hit, and filtering out non-syntenic hits. For more distant species reciprocal-best BLASTP hits are used. Note that the absence of an ortholog in the table below may reflect incomplete annotations in the other species rather than a true absence of the orthologous gene.
MouseRatZebrafishD. melanogasterC. elegansS. cerevisiae
Genome BrowserGenome BrowserNo orthologGenome BrowserNo orthologNo ortholog
Gene Details     
Gene Sorter     
MGIRGD Ensembl  
Protein SequenceProtein Sequence Protein Sequence  
AlignmentAlignment Alignment  

-  Gene Ontology (GO) Annotations with Structured Vocabulary
  Molecular Function:
GO:0005515 protein binding
GO:0019904 protein domain specific binding
GO:0042802 identical protein binding
GO:0044877 macromolecular complex binding
GO:0045296 cadherin binding
GO:0090541 MIT domain binding

Biological Process:
GO:0007049 cell cycle
GO:0008104 protein localization
GO:0009838 abscission
GO:0015031 protein transport
GO:0019076 viral release from host cell
GO:0036258 multivesicular body assembly
GO:0043312 neutrophil degranulation
GO:0045184 establishment of protein localization
GO:0045862 positive regulation of proteolysis
GO:0046745 viral capsid secondary envelopment
GO:0048672 positive regulation of collateral sprouting
GO:0051301 cell division
GO:0061640 cytoskeleton-dependent cytokinesis
GO:1904903 ESCRT III complex disassembly

Cellular Component:
GO:0005576 extracellular region
GO:0005634 nucleus
GO:0005635 nuclear envelope
GO:0005737 cytoplasm
GO:0005793 endoplasmic reticulum-Golgi intermediate compartment
GO:0005813 centrosome
GO:0005815 microtubule organizing center
GO:0005829 cytosol
GO:0005856 cytoskeleton
GO:0030496 midbody
GO:0031410 cytoplasmic vesicle
GO:0035578 azurophil granule lumen
GO:0043231 intracellular membrane-bounded organelle
GO:0070062 extracellular exosome
GO:0090543 Flemming body
GO:0000785 chromatin


-  Descriptions from all associated GenBank mRNAs
  LF384451 - JP 2014500723-A/191954: Polycomb-Associated Non-Coding RNAs.
MA620028 - JP 2018138019-A/191954: Polycomb-Associated Non-Coding RNAs.
AK057258 - Homo sapiens cDNA FLJ32696 fis, clone TESTI2000358.
AK293040 - Homo sapiens cDNA FLJ77725 complete cds.
AK291388 - Homo sapiens cDNA FLJ78449 complete cds.
AK297336 - Homo sapiens cDNA FLJ52563 complete cds.
BC103745 - Homo sapiens KIAA0174, mRNA (cDNA clone MGC:117220 IMAGE:6009274), complete cds.
AK295066 - Homo sapiens cDNA FLJ52541 complete cds.
AK301918 - Homo sapiens cDNA FLJ59095 complete cds.
AK293022 - Homo sapiens cDNA FLJ78415 complete cds.
AK297088 - Homo sapiens cDNA FLJ52560 complete cds.
AK057902 - Homo sapiens cDNA FLJ25173 fis, clone CBR08822.
AB097052 - Homo sapiens mRNA for putative MAPK activating protein, complete cds, clone: PM28.
D79996 - Homo sapiens KIAA0174 mRNA, partial cds.
BC000430 - Homo sapiens KIAA0174, mRNA (cDNA clone MGC:8358 IMAGE:2819828), complete cds.
BC004359 - Homo sapiens KIAA0174, mRNA (cDNA clone MGC:4312 IMAGE:2819828), complete cds.
BC000116 - Homo sapiens KIAA0174, mRNA (cDNA clone MGC:3110 IMAGE:3350789), complete cds.
JD203204 - Sequence 184228 from Patent EP1572962.
HQ447682 - Synthetic construct Homo sapiens clone IMAGE:100071701; CCSB003143_01 KIAA0174 (KIAA0174) gene, encodes complete protein.
KJ892851 - Synthetic construct Homo sapiens clone ccsbBroadEn_02245 IST1 gene, encodes complete protein.
KJ902023 - Synthetic construct Homo sapiens clone ccsbBroadEn_11417 IST1 gene, encodes complete protein.
CU674046 - Synthetic construct Homo sapiens gateway clone IMAGE:100018987 5' read KIAA0174 mRNA.
CU674216 - Synthetic construct Homo sapiens gateway clone IMAGE:100017102 5' read KIAA0174 mRNA.
AB383784 - Synthetic construct DNA, clone: pF1KSDA0174, Homo sapiens KIAA0174 gene for KIAA0174 protein, complete cds, without stop codon, in Flexi system.
DQ587024 - Homo sapiens piRNA piR-54136, complete sequence.
DQ590325 - Homo sapiens piRNA piR-57437, complete sequence.
AL355695 - Homo sapiens EST from clone 114069, full insert.
LF374504 - JP 2014500723-A/182007: Polycomb-Associated Non-Coding RNAs.
MA610081 - JP 2018138019-A/182007: Polycomb-Associated Non-Coding RNAs.
LF374502 - JP 2014500723-A/182005: Polycomb-Associated Non-Coding RNAs.
MA610079 - JP 2018138019-A/182005: Polycomb-Associated Non-Coding RNAs.
JD530163 - Sequence 511187 from Patent EP1572962.
JD503687 - Sequence 484711 from Patent EP1572962.
JD132296 - Sequence 113320 from Patent EP1572962.
JD251301 - Sequence 232325 from Patent EP1572962.
LF374501 - JP 2014500723-A/182004: Polycomb-Associated Non-Coding RNAs.
MA610078 - JP 2018138019-A/182004: Polycomb-Associated Non-Coding RNAs.
JD420817 - Sequence 401841 from Patent EP1572962.
JD400845 - Sequence 381869 from Patent EP1572962.
LF374500 - JP 2014500723-A/182003: Polycomb-Associated Non-Coding RNAs.
MA610077 - JP 2018138019-A/182003: Polycomb-Associated Non-Coding RNAs.
JD340622 - Sequence 321646 from Patent EP1572962.
JD111451 - Sequence 92475 from Patent EP1572962.
JD344556 - Sequence 325580 from Patent EP1572962.
JD482503 - Sequence 463527 from Patent EP1572962.
JD075363 - Sequence 56387 from Patent EP1572962.
JD450427 - Sequence 431451 from Patent EP1572962.
DQ591076 - Homo sapiens piRNA piR-58188, complete sequence.
JD557708 - Sequence 538732 from Patent EP1572962.
JD225811 - Sequence 206835 from Patent EP1572962.
JD433909 - Sequence 414933 from Patent EP1572962.
JD302109 - Sequence 283133 from Patent EP1572962.
JD307716 - Sequence 288740 from Patent EP1572962.
JD274205 - Sequence 255229 from Patent EP1572962.
JD492853 - Sequence 473877 from Patent EP1572962.
JD237430 - Sequence 218454 from Patent EP1572962.
BC038350 - Homo sapiens cDNA clone IMAGE:4537875, with apparent retained intron.

-  Biochemical and Signaling Pathways
  Reactome (by CSHL, EBI, and GO)

Protein P53990 (Reactome details) participates in the following event(s):

R-HSA-6798751 Exocytosis of azurophil granule lumen proteins
R-HSA-6798695 Neutrophil degranulation
R-HSA-168249 Innate Immune System
R-HSA-168256 Immune System

-  Other Names for This Gene
  Alternate Gene Symbols: ENST00000378799.1, ENST00000378799.10, ENST00000378799.2, ENST00000378799.3, ENST00000378799.4, ENST00000378799.5, ENST00000378799.6, ENST00000378799.7, ENST00000378799.8, ENST00000378799.9, IST1_HUMAN, KIAA0174, NM_001270975, P53990, Q3SYM4, Q9BQ81, Q9BWN2, uc002fbm.1, uc002fbm.2, uc002fbm.3, uc002fbm.4
UCSC ID: ENST00000378799.11
RefSeq Accession: NM_001270975
Protein: P53990 (aka IST1_HUMAN)
CCDS: CCDS10905.1, CCDS59272.1, CCDS59271.1

-  Methods, Credits, and Use Restrictions
  Click here for details on how this gene model was made and data restrictions if any.