Human Gene KRT33A (ENST00000007735.4) from GENCODE V44
  Description: Homo sapiens keratin 33A (KRT33A), mRNA. (from RefSeq NM_004138)
RefSeq Summary (NM_004138): This gene encodes a member of the keratin gene family. This gene is one of multiple type I hair keratin genes that are clustered in a region of chromosome 17q12-q21 and have the same direction of transcription. As a type I hair keratin, the encoded protein is an acidic protein which heterodimerizes with type II keratins to form hair and nails. There are two isoforms of this protein, encoded by two separate genes, keratin 33A and keratin 33B. [provided by RefSeq, May 2012].
Gencode Transcript: ENST00000007735.4
Gencode Gene: ENSG00000006059.4
Transcript (Including UTRs)
   Position: hg38 chr17:41,346,092-41,350,828 Size: 4,737 Total Exon Count: 7 Strand: -
Coding Region
   Position: hg38 chr17:41,346,119-41,350,767 Size: 4,649 Coding Exon Count: 7 

Page IndexSequence and LinksUniProtKB CommentsPrimersCTDRNA-Seq Expression
Microarray ExpressionRNA StructureProtein StructureOther SpeciesGO AnnotationsmRNA Descriptions
PathwaysOther NamesMethods
Data last updated at UCSC: 2023-08-18 00:09:47

-  Sequence and Links to Tools and Databases
 
Genomic Sequence (chr17:41,346,092-41,350,828)mRNA (may differ from genome)Protein (404 aa)
Gene SorterGenome BrowserOther Species FASTAGene interactionsTable SchemaAlphaFold
BioGPSEnsemblEntrez GeneExonPrimerGencodeGeneCards
HGNCHPRDLynxMalacardsMGIneXtProt
OMIMPubMedReactomeUniProtKBWikipediaBioGrid CRISPR DB

-  Comments and Description Text from UniProtKB
  ID: KT33A_HUMAN
DESCRIPTION: RecName: Full=Keratin, type I cuticular Ha3-I; AltName: Full=Hair keratin, type I Ha3-I; AltName: Full=Keratin-33A; Short=K33A;
TISSUE SPECIFICITY: Expressed in the hair follicles.
MISCELLANEOUS: There are two types of hair/microfibrillar keratin, I (acidic) and II (neutral to basic).
SIMILARITY: Belongs to the intermediate filament family.

-  Primer design for this transcript
 

Primer3Plus can design qPCR Primers that straddle exon-exon-junctions, which amplify only cDNA, not genomic DNA.
Click here to load the transcript sequence and exon structure into Primer3Plus

Exonprimer can design one pair of Sanger sequencing primers around every exon, located in non-genic sequence.
Click here to open Exonprimer with this transcript

To design primers for a non-coding sequence, zoom to a region of interest and select from the drop-down menu: View > In External Tools > Primer3


-  Comparative Toxicogenomics Database (CTD)
  The following chemicals interact with this gene

-  RNA-Seq Expression Data from GTEx (53 Tissues, 570 Donors)
  Highest median expression: 3.10 RPKM in Testis
Total median expression: 4.10 RPKM



View in GTEx track of Genome Browser    View at GTEx portal     View GTEx Body Map

+  Microarray Expression Data
  Press "+" in the title bar above to open this section.

-  mRNA Secondary Structure of 3' and 5' UTRs
 
RegionFold EnergyBasesEnergy/Base
Display As
5' UTR -10.9061-0.179 Picture PostScript Text
3' UTR -2.9027-0.107 Picture PostScript Text

The RNAfold program from the Vienna RNA Package is used to perform the secondary structure predictions and folding calculations. The estimated folding energy is in kcal/mol. The more negative the energy, the more secondary structure the RNA is likely to have.

-  Protein Domain and Structure Information
  InterPro Domains: Graphical view of domain structure
IPR016044 - F
IPR001664 - IF
IPR018039 - Intermediate_filament_CS
IPR002957 - Keratin_I

Pfam Domains:
PF00038 - Intermediate filament protein

ModBase Predicted Comparative 3D Structure on O76009
FrontTopSide
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-  Orthologous Genes in Other Species
  Orthologies between human, mouse, and rat are computed by taking the best BLASTP hit, and filtering out non-syntenic hits. For more distant species reciprocal-best BLASTP hits are used. Note that the absence of an ortholog in the table below may reflect incomplete annotations in the other species rather than a true absence of the orthologous gene.
MouseRatZebrafishD. melanogasterC. elegansS. cerevisiae
Genome BrowserGenome BrowserNo orthologNo orthologNo orthologNo ortholog
Gene Details     
Gene Sorter     
MGIRGD    
Protein SequenceProtein Sequence    
AlignmentAlignment    

-  Gene Ontology (GO) Annotations with Structured Vocabulary
  Molecular Function:
GO:0005198 structural molecule activity

Biological Process:
GO:0031424 keratinization
GO:0070268 cornification

Cellular Component:
GO:0005615 extracellular space
GO:0005829 cytosol
GO:0005882 intermediate filament


-  Descriptions from all associated GenBank mRNAs
  AJ633621 - Homo sapiens mRNA for keratin hHa3-I (KRTHA3A gene).
BC069135 - Homo sapiens keratin 33A, mRNA (cDNA clone MGC:95416 IMAGE:7216991), complete cds.
AK314088 - Homo sapiens cDNA, FLJ94763, highly similar to Homo sapiens keratin, hair, acidic, 3A (KRTHA3A), mRNA.
CU679547 - Synthetic construct Homo sapiens gateway clone IMAGE:100019255 5' read KRT33B mRNA.
JD157670 - Sequence 138694 from Patent EP1572962.

-  Biochemical and Signaling Pathways
  Reactome (by CSHL, EBI, and GO)

Protein O76009 (Reactome details) participates in the following event(s):

R-HSA-6805546 Keratin type I binds keratin type II
R-HSA-6805573 Keratin type I/type II heterodimers form tetramers
R-HSA-6806613 Keratin tetramers bind to form unit length filaments
R-HSA-6805567 Keratinization
R-HSA-1266738 Developmental Biology

-  Other Names for This Gene
  Alternate Gene Symbols: B2RA87, ENST00000007735.1, ENST00000007735.2, ENST00000007735.3, HHA3-I, HKA3A, KRTHA3A, KT33A_HUMAN, NM_004138, O76009, Q6NTB9, Q6ZZB9, uc002hwk.1, uc002hwk.2, uc002hwk.3, uc002hwk.4
UCSC ID: ENST00000007735.4
RefSeq Accession: NM_004138
Protein: O76009 (aka KT33A_HUMAN)
CCDS: CCDS11388.1

-  Methods, Credits, and Use Restrictions
  Click here for details on how this gene model was made and data restrictions if any.