Human Gene ZNF135 (ENST00000401053.8) from GENCODE V44
  Description: Homo sapiens zinc finger protein 135 (ZNF135), transcript variant 1, mRNA. (from RefSeq NM_007134)
Gencode Transcript: ENST00000401053.8
Gencode Gene: ENSG00000176293.20
Transcript (Including UTRs)
   Position: hg38 chr19:58,059,239-58,069,755 Size: 10,517 Total Exon Count: 4 Strand: +
Coding Region
   Position: hg38 chr19:58,059,242-58,068,461 Size: 9,220 Coding Exon Count: 4 

Page IndexSequence and LinksUniProtKB CommentsPrimersCTDRNA-Seq Expression
Microarray ExpressionRNA StructureProtein StructureOther SpeciesGO AnnotationsmRNA Descriptions
PathwaysOther NamesMethods
Data last updated at UCSC: 2023-08-18 00:09:47

-  Sequence and Links to Tools and Databases
Genomic Sequence (chr19:58,059,239-58,069,755)mRNA (may differ from genome)Protein (682 aa)
Gene SorterGenome BrowserOther Species FASTAGene interactionsTable SchemaAlphaFold
BioGPSEnsemblEntrez GeneExonPrimerGencodeGeneCards
PubMedReactomeUniProtKBBioGrid CRISPR DB

-  Comments and Description Text from UniProtKB
DESCRIPTION: RecName: Full=Zinc finger protein 135; AltName: Full=Zinc finger protein 61; AltName: Full=Zinc finger protein 78-like 1;
FUNCTION: Plays a role in the regulation of cell morphology and cytoskeletal organization. May be involved in transcriptional regulation.
SUBCELLULAR LOCATION: Nucleus (Potential).
SIMILARITY: Belongs to the krueppel C2H2-type zinc-finger protein family.
SIMILARITY: Contains 16 C2H2-type zinc fingers.
SIMILARITY: Contains 1 KRAB domain.
SEQUENCE CAUTION: Sequence=AAC50254.1; Type=Frameshift; Positions=207, 558, 563;

-  Primer design for this transcript

Primer3Plus can design qPCR Primers that straddle exon-exon-junctions, which amplify only cDNA, not genomic DNA.
Click here to load the transcript sequence and exon structure into Primer3Plus

Exonprimer can design one pair of Sanger sequencing primers around every exon, located in non-genic sequence.
Click here to open Exonprimer with this transcript

To design primers for a non-coding sequence, zoom to a region of interest and select from the drop-down menu: View > In External Tools > Primer3

-  Comparative Toxicogenomics Database (CTD)
  The following chemicals interact with this gene

-  RNA-Seq Expression Data from GTEx (53 Tissues, 570 Donors)
  Highest median expression: 5.92 RPKM in Ovary
Total median expression: 114.62 RPKM

View in GTEx track of Genome Browser    View at GTEx portal     View GTEx Body Map

+  Microarray Expression Data
  Press "+" in the title bar above to open this section.

-  mRNA Secondary Structure of 3' and 5' UTRs
RegionFold EnergyBasesEnergy/Base
Display As
3' UTR -336.801294-0.260 Picture PostScript Text

The RNAfold program from the Vienna RNA Package is used to perform the secondary structure predictions and folding calculations. The estimated folding energy is in kcal/mol. The more negative the energy, the more secondary structure the RNA is likely to have.

-  Protein Domain and Structure Information
  InterPro Domains: Graphical view of domain structure
IPR001909 - Krueppel-associated_box
IPR007087 - Znf_C2H2
IPR015880 - Znf_C2H2-like
IPR013087 - Znf_C2H2/integrase_DNA-bd

Pfam Domains:
PF01352 - KRAB box

ModBase Predicted Comparative 3D Structure on P52742
The pictures above may be empty if there is no ModBase structure for the protein. The ModBase structure frequently covers just a fragment of the protein. You may be asked to log onto ModBase the first time you click on the pictures. It is simplest after logging in to just click on the picture again to get to the specific info on that model.

-  Orthologous Genes in Other Species
  Orthologies between human, mouse, and rat are computed by taking the best BLASTP hit, and filtering out non-syntenic hits. For more distant species reciprocal-best BLASTP hits are used. Note that the absence of an ortholog in the table below may reflect incomplete annotations in the other species rather than a true absence of the orthologous gene.
MouseRatZebrafishD. melanogasterC. elegansS. cerevisiae
Genome BrowserNo orthologNo orthologNo orthologNo orthologNo ortholog
Gene Details     
Gene Sorter     
Protein Sequence     

-  Gene Ontology (GO) Annotations with Structured Vocabulary
  Molecular Function:
GO:0000981 RNA polymerase II transcription factor activity, sequence-specific DNA binding
GO:0003676 nucleic acid binding
GO:0003677 DNA binding
GO:0003700 transcription factor activity, sequence-specific DNA binding
GO:0046872 metal ion binding

Biological Process:
GO:0006351 transcription, DNA-templated
GO:0006355 regulation of transcription, DNA-templated
GO:0006357 regulation of transcription from RNA polymerase II promoter
GO:0007010 cytoskeleton organization
GO:0022604 regulation of cell morphogenesis

Cellular Component:
GO:0005622 intracellular
GO:0005634 nucleus

-  Descriptions from all associated GenBank mRNAs
  AK098011 - Homo sapiens cDNA FLJ40692 fis, clone THYMU2024748, highly similar to ZINC FINGER PROTEIN 135.
AK297226 - Homo sapiens cDNA FLJ56378 complete cds, highly similar to Homo sapiens zinc finger protein 135 (ZNF135), mRNA.
BC046434 - Homo sapiens zinc finger protein 135, mRNA (cDNA clone MGC:42543 IMAGE:4824328), complete cds.
AK295110 - Homo sapiens cDNA FLJ61260 complete cds, highly similar to Homo sapiens zinc finger protein 135 (ZNF135), mRNA.
BX647775 - Homo sapiens mRNA; cDNA DKFZp686O1759 (from clone DKFZp686O1759).
AK094202 - Homo sapiens cDNA FLJ36883 fis, clone BLADE2000389, highly similar to ZINC FINGER PROTEIN 135.
AL833637 - Homo sapiens mRNA; cDNA DKFZp686L1079 (from clone DKFZp686L1079).
AK095796 - Homo sapiens cDNA FLJ38477 fis, clone FEBRA2022601.
AB527474 - Synthetic construct DNA, clone: pF1KB7896, Homo sapiens ZNF135 gene for zinc finger protein 135, without stop codon, in Flexi system.
U09413 - Human zinc finger protein ZNF135 mRNA, complete cds.
X65231 - H.sapiens mRNA for Zinc-finger protein (ZNFpT3).
JD050175 - Sequence 31199 from Patent EP1572962.
JD098173 - Sequence 79197 from Patent EP1572962.
JD416069 - Sequence 397093 from Patent EP1572962.
JD470315 - Sequence 451339 from Patent EP1572962.
JD225618 - Sequence 206642 from Patent EP1572962.
JD496817 - Sequence 477841 from Patent EP1572962.
JD453720 - Sequence 434744 from Patent EP1572962.
JD492833 - Sequence 473857 from Patent EP1572962.
JD181294 - Sequence 162318 from Patent EP1572962.
JD554008 - Sequence 535032 from Patent EP1572962.
JD346977 - Sequence 328001 from Patent EP1572962.
JD497126 - Sequence 478150 from Patent EP1572962.
JD276779 - Sequence 257803 from Patent EP1572962.
JD396407 - Sequence 377431 from Patent EP1572962.
JD385617 - Sequence 366641 from Patent EP1572962.
JD413711 - Sequence 394735 from Patent EP1572962.
JD276650 - Sequence 257674 from Patent EP1572962.
JD423816 - Sequence 404840 from Patent EP1572962.

-  Biochemical and Signaling Pathways
  Reactome (by CSHL, EBI, and GO)

Protein P52742 (Reactome details) participates in the following event(s):

R-HSA-975040 KRAB-ZNF / KAP Interaction
R-HSA-212436 Generic Transcription Pathway
R-HSA-73857 RNA Polymerase II Transcription
R-HSA-74160 Gene expression (Transcription)

-  Other Names for This Gene
  Alternate Gene Symbols: ENST00000401053.1, ENST00000401053.2, ENST00000401053.3, ENST00000401053.4, ENST00000401053.5, ENST00000401053.6, ENST00000401053.7, NM_007134, P52742, Q5U5L3, uc002qrg.1, uc002qrg.2, uc002qrg.3, uc002qrg.4, ZN135_HUMAN, ZNF61, ZNF78L1
UCSC ID: ENST00000401053.8
RefSeq Accession: NM_007134
Protein: P52742 (aka ZN135_HUMAN or Z135_HUMAN)
CCDS: CCDS12970.2, CCDS54329.1

-  Methods, Credits, and Use Restrictions
  Click here for details on how this gene model was made and data restrictions if any.