Human Gene SLC27A5 (ENST00000263093.7) from GENCODE V44
Description: Homo sapiens solute carrier family 27 member 5 (SLC27A5), transcript variant 1, mRNA. (from RefSeq NM_012254) RefSeq Summary (NM_012254): The protein encoded by this gene is an isozyme of very long-chain acyl-CoA synthetase (VLCS). It is capable of activating very long-chain fatty-acids containing 24- and 26-carbons. It is expressed in liver and associated with endoplasmic reticulum but not with peroxisomes. Its primary role is in fatty acid elongation or complex lipid synthesis rather than in degradation. This gene has a mouse ortholog. [provided by RefSeq, Jul 2008]. Gencode Transcript: ENST00000263093.7 Gencode Gene: ENSG00000083807.10 Transcript (Including UTRs) Position: hg38 chr19:58,498,333-58,511,992 Size: 13,660 Total Exon Count: 10 Strand: - Coding Region Position: hg38 chr19:58,498,515-58,511,955 Size: 13,441 Coding Exon Count: 10
ID:S27A5_HUMAN DESCRIPTION: RecName: Full=Bile acyl-CoA synthetase; Short=BACS; EC=6.2.1.7; AltName: Full=Bile acid-CoA ligase; Short=BA-CoA ligase; Short=BAL; AltName: Full=Cholate--CoA ligase; AltName: Full=Fatty acid transport protein 5; Short=FATP-5; AltName: Full=Fatty-acid-coenzyme A ligase, very long-chain 3; AltName: Full=Solute carrier family 27 member 5; AltName: Full=Very long-chain acyl-CoA synthetase homolog 2; Short=VLCS-H2; Short=VLCSH2; AltName: Full=Very long-chain acyl-CoA synthetase-related protein; Short=VLACS-related; Short=VLACSR; FUNCTION: Acyl-CoA synthetase involved in bile acid metabolism. Proposed to catalyze the first step in the conjugation of C24 bile acids (choloneates) to glycine and taurine before excretion into bile canaliculi by activating them to their CoA thioesters. Seems to activate secondary bile acids entering the liver from the enterohepatic circulation. In vitro, also activates 3-alpha,7- alpha,12-alpha-trihydroxy-5-beta-cholestanate (THCA), the C27 precursor of cholic acid deriving from the de novo synthesis from cholesterol. CATALYTIC ACTIVITY: ATP + cholate + CoA = AMP + diphosphate + choloyl-CoA. CATALYTIC ACTIVITY: ATP + (25R)-3-alpha,7-alpha,12-alpha- trihydroxy-5-beta-cholestan-26-oate + CoA = AMP + diphosphate + (25R)-3-alpha,7-alpha,12-alpha-trihydroxy-5-beta-cholestanoyl-CoA. SUBCELLULAR LOCATION: Endoplasmic reticulum membrane; Multi-pass membrane protein. TISSUE SPECIFICITY: Predominantly expressed in liver. SIMILARITY: Belongs to the ATP-dependent AMP-binding enzyme family.
The RNAfold program from the Vienna RNA Package is used to perform the secondary structure predictions and folding calculations. The estimated folding energy is in kcal/mol. The more negative the energy, the more secondary structure the RNA is likely to have.
ModBase Predicted Comparative 3D Structure on Q9Y2P5
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Orthologous Genes in Other Species
Orthologies between human, mouse, and rat are computed by taking the best BLASTP hit, and filtering out non-syntenic hits. For more distant species reciprocal-best BLASTP hits are used. Note that the absence of an ortholog in the table below may reflect incomplete annotations in the other species rather than a true absence of the orthologous gene.
Biological Process: GO:0000038 very long-chain fatty acid metabolic process GO:0001676 long-chain fatty acid metabolic process GO:0006629 lipid metabolic process GO:0006631 fatty acid metabolic process GO:0006642 triglyceride mobilization GO:0006699 bile acid biosynthetic process GO:0008152 metabolic process GO:0008206 bile acid metabolic process GO:0015721 bile acid and bile salt transport GO:0015908 fatty acid transport GO:0015911 plasma membrane long-chain fatty acid transport GO:0046951 ketone body biosynthetic process
Cellular Component: GO:0005783 endoplasmic reticulum GO:0005789 endoplasmic reticulum membrane GO:0009925 basal plasma membrane GO:0016020 membrane GO:0016021 integral component of membrane GO:0030176 integral component of endoplasmic reticulum membrane GO:0032991 macromolecular complex GO:0043231 intracellular membrane-bounded organelle
Descriptions from all associated GenBank mRNAs
AK026640 - Homo sapiens cDNA: FLJ22987 fis, clone KAT11802. AK123036 - Homo sapiens cDNA FLJ41040 fis, clone LIVER1000017, highly similar to Bile acyl-CoA synthetase (EC 6.2.1.7). AF064255 - Homo sapiens very long-chain acyl-CoA synthetase homolog 2 mRNA, complete cds. AK298446 - Homo sapiens cDNA FLJ55741 complete cds, moderately similar to Bile acyl-CoA synthetase (EC 6.2.1.7). JD159166 - Sequence 140190 from Patent EP1572962. JD257349 - Sequence 238373 from Patent EP1572962. JD334387 - Sequence 315411 from Patent EP1572962. BC146387 - Synthetic construct Homo sapiens clone IMAGE:100015134, MGC:180217 solute carrier family 27 (fatty acid transporter), member 5 (SLC27A5) mRNA, encodes complete protein. BC148807 - Synthetic construct Homo sapiens clone IMAGE:100015855, MGC:183197 solute carrier family 27 (fatty acid transporter), member 5 (SLC27A5) mRNA, encodes complete protein. AB527212 - Synthetic construct DNA, clone: pF1KE0364, Homo sapiens SLC27A5 gene for solute carrier family 27 (fatty acid transporter), member 5, without stop codon, in Flexi system. AB208931 - Homo sapiens mRNA for solute carrier family 27 (fatty acid transporter), member 5 variant protein.
Biochemical and Signaling Pathways
KEGG - Kyoto Encyclopedia of Genes and Genomes hsa00120 - Primary bile acid biosynthesis hsa01100 - Metabolic pathways hsa03320 - PPAR signaling pathway
BioCyc Knowledge Library PWY-6061 - bile acid biosynthesis, neutral pathway
Reactome (by CSHL, EBI, and GO)
Protein Q9Y2P5 (Reactome details) participates in the following event(s):
R-HSA-159425 Cytosolic cholate and chenodeoxycholate are conjugated with Coenzyme A (SLC27A5 BACS) R-HSA-192137 THCA is conjugated with Coenzyme A (SLC27A5 BACS) R-HSA-193407 DHCA is conjugated with Coenzyme A (SLC27A5 BACS) R-HSA-193711 3,7,24THCA is conjugated with Coenzyme A (SLC27A5 BACS) R-HSA-193766 TetraHCA is conjugated with Coenzyme A (SLC27A5 BACS) R-HSA-159418 Recycling of bile acids and salts R-HSA-193368 Synthesis of bile acids and bile salts via 7alpha-hydroxycholesterol R-HSA-193775 Synthesis of bile acids and bile salts via 24-hydroxycholesterol R-HSA-194068 Bile acid and bile salt metabolism R-HSA-192105 Synthesis of bile acids and bile salts R-HSA-8957322 Metabolism of steroids R-HSA-556833 Metabolism of lipids R-HSA-1430728 Metabolism
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