Human Gene PROM2 (ENST00000317668.8) from GENCODE V44
  Description: Homo sapiens prominin 2 (PROM2), transcript variant 3, mRNA. (from RefSeq NM_144707)
RefSeq Summary (NM_144707): This gene encodes a member of the prominin family of pentaspan membrane glycoproteins. The encoded protein localizes to basal epithelial cells and may be involved in the organization of plasma membrane microdomains. Alternative splicing results in multiple transcript variants. [provided by RefSeq, Oct 2009].
Gencode Transcript: ENST00000317668.8
Gencode Gene: ENSG00000155066.16
Transcript (Including UTRs)
   Position: hg38 chr2:95,274,453-95,291,308 Size: 16,856 Total Exon Count: 24 Strand: +
Coding Region
   Position: hg38 chr2:95,274,586-95,288,996 Size: 14,411 Coding Exon Count: 23 

Page IndexSequence and LinksUniProtKB CommentsPrimersMalaCardsCTD
RNA-Seq ExpressionMicroarray ExpressionRNA StructureProtein StructureOther SpeciesGO Annotations
mRNA DescriptionsOther NamesMethods
Data last updated at UCSC: 2023-08-18 00:09:47

-  Sequence and Links to Tools and Databases
 
Genomic Sequence (chr2:95,274,453-95,291,308)mRNA (may differ from genome)Protein (834 aa)
Gene SorterGenome BrowserOther Species FASTAGene interactionsTable SchemaAlphaFold
BioGPSEnsemblEntrez GeneExonPrimerGencodeGeneCards
HGNCHPRDLynxMalacardsMGIneXtProt
OMIMPubMedUniProtKBBioGrid CRISPR DB

-  Comments and Description Text from UniProtKB
  ID: PROM2_HUMAN
DESCRIPTION: RecName: Full=Prominin-2; Short=PROM-2; AltName: Full=Prominin-like protein 2; Short=hPROML2; Flags: Precursor;
SUBUNIT: Binds cholesterol (By similarity).
SUBCELLULAR LOCATION: Apical cell membrane; Multi-pass membrane protein (By similarity). Basolateral cell membrane; Multi-pass membrane protein (By similarity). Cell projection, microvillus membrane; Multi-pass membrane protein (By similarity). Cell projection, cilium membrane; Multi-pass membrane protein (By similarity). Note=Colocalizes with PROM1. Associates with membrane in a cholesterol-dependent manner. Localizes to the apical and basolateral membranes of epithelial cells (By similarity).
TISSUE SPECIFICITY: Present in saliva within small membrane particles (at protein level). Expressed in kidney, prostate, trachea, esophagus, salivary gland, thyroid gland, mammary gland adrenal gland, placenta, stomach, spinal cord and liver. In submucosal tumor, expressed in spindle-shaped or stellate stromal cells. Expressed in prostate cancer cell lines.
PTM: Glycosylated (By similarity).
SIMILARITY: Belongs to the prominin family.
SEQUENCE CAUTION: Sequence=BAC03657.1; Type=Erroneous initiation;

-  Primer design for this transcript
 

Primer3Plus can design qPCR Primers that straddle exon-exon-junctions, which amplify only cDNA, not genomic DNA.
Click here to load the transcript sequence and exon structure into Primer3Plus

Exonprimer can design one pair of Sanger sequencing primers around every exon, located in non-genic sequence.
Click here to open Exonprimer with this transcript

To design primers for a non-coding sequence, zoom to a region of interest and select from the drop-down menu: View > In External Tools > Primer3


-  MalaCards Disease Associations
  MalaCards Gene Search: PROM2
Diseases sorted by gene-association score: chromophobe renal cell carcinoma (3)

-  Comparative Toxicogenomics Database (CTD)
  The following chemicals interact with this gene           more ... click here to view the complete list

-  RNA-Seq Expression Data from GTEx (53 Tissues, 570 Donors)
  Highest median expression: 61.92 RPKM in Esophagus - Mucosa
Total median expression: 373.91 RPKM



View in GTEx track of Genome Browser    View at GTEx portal     View GTEx Body Map

+  Microarray Expression Data
  Press "+" in the title bar above to open this section.

-  mRNA Secondary Structure of 3' and 5' UTRs
 
RegionFold EnergyBasesEnergy/Base
Display As
5' UTR -47.10133-0.354 Picture PostScript Text
3' UTR -415.601186-0.350 Picture PostScript Text

The RNAfold program from the Vienna RNA Package is used to perform the secondary structure predictions and folding calculations. The estimated folding energy is in kcal/mol. The more negative the energy, the more secondary structure the RNA is likely to have.

-  Protein Domain and Structure Information
  InterPro Domains: Graphical view of domain structure
IPR008795 - Prominin

Pfam Domains:
PF05478 - Prominin

ModBase Predicted Comparative 3D Structure on Q8N271
FrontTopSide
The pictures above may be empty if there is no ModBase structure for the protein. The ModBase structure frequently covers just a fragment of the protein. You may be asked to log onto ModBase the first time you click on the pictures. It is simplest after logging in to just click on the picture again to get to the specific info on that model.

-  Orthologous Genes in Other Species
  Orthologies between human, mouse, and rat are computed by taking the best BLASTP hit, and filtering out non-syntenic hits. For more distant species reciprocal-best BLASTP hits are used. Note that the absence of an ortholog in the table below may reflect incomplete annotations in the other species rather than a true absence of the orthologous gene.
MouseRatZebrafishD. melanogasterC. elegansS. cerevisiae
Genome BrowserGenome BrowserNo orthologNo orthologNo orthologNo ortholog
Gene Details     
Gene Sorter     
MGIRGD    
Protein SequenceProtein Sequence    
AlignmentAlignment    

-  Gene Ontology (GO) Annotations with Structured Vocabulary
  Molecular Function:
GO:0015485 cholesterol binding

Biological Process:
GO:0001934 positive regulation of protein phosphorylation
GO:0031346 positive regulation of cell projection organization
GO:0043087 regulation of GTPase activity
GO:0048550 negative regulation of pinocytosis
GO:2001287 negative regulation of caveolin-mediated endocytosis
GO:2000369 regulation of clathrin-dependent endocytosis

Cellular Component:
GO:0005886 plasma membrane
GO:0005887 integral component of plasma membrane
GO:0005902 microvillus
GO:0005929 cilium
GO:0009986 cell surface
GO:0016020 membrane
GO:0016021 integral component of membrane
GO:0016323 basolateral plasma membrane
GO:0016324 apical plasma membrane
GO:0031410 cytoplasmic vesicle
GO:0031528 microvillus membrane
GO:0042995 cell projection
GO:0044393 microspike
GO:0060170 ciliary membrane
GO:0070062 extracellular exosome
GO:0071914 prominosome
GO:0045121 membrane raft


-  Descriptions from all associated GenBank mRNAs
  AF245303 - Homo sapiens prominin-2 variant A mRNA, complete cds; alternatively spliced.
AF245304 - Homo sapiens prominin-2 variant B mRNA, complete cds; alternatively spliced.
BC113877 - Homo sapiens prominin 2, mRNA (cDNA clone IMAGE:40054100), complete cds.
BC114525 - Homo sapiens prominin 2, mRNA (cDNA clone MGC:138714 IMAGE:40054105), complete cds.
AK091175 - Homo sapiens cDNA FLJ33856 fis, clone CTONG2005913, moderately similar to Mus musculus prominin-like protein mRNA.
AX746828 - Sequence 353 from Patent EP1308459.
AK290366 - Homo sapiens cDNA FLJ77932 complete cds.
AY358377 - Homo sapiens clone DNA92217 PROM2 (UNQ2521) mRNA, complete cds.
BC025736 - Homo sapiens prominin 2, mRNA (cDNA clone IMAGE:5209597), with apparent retained intron.
AK091408 - Homo sapiens cDNA FLJ34089 fis, clone FCBBF3006249, moderately similar to Mus musculus prominin-like protein mRNA.
AX746963 - Sequence 488 from Patent EP1308459.
JD496584 - Sequence 477608 from Patent EP1572962.
AK313267 - Homo sapiens cDNA, FLJ93777, highly similar to Homo sapiens prominin 2 (PROM2), mRNA.
JD192887 - Sequence 173911 from Patent EP1572962.
JD426049 - Sequence 407073 from Patent EP1572962.
JD122671 - Sequence 103695 from Patent EP1572962.
KJ905627 - Synthetic construct Homo sapiens clone ccsbBroadEn_15266 PROM2-like gene, encodes complete protein.
JD445038 - Sequence 426062 from Patent EP1572962.
JD073729 - Sequence 54753 from Patent EP1572962.
JD043787 - Sequence 24811 from Patent EP1572962.
JD247745 - Sequence 228769 from Patent EP1572962.
JD065566 - Sequence 46590 from Patent EP1572962.
JD331338 - Sequence 312362 from Patent EP1572962.
JD322297 - Sequence 303321 from Patent EP1572962.
JD156422 - Sequence 137446 from Patent EP1572962.
JD231452 - Sequence 212476 from Patent EP1572962.
JD400216 - Sequence 381240 from Patent EP1572962.
JD452906 - Sequence 433930 from Patent EP1572962.
JD474287 - Sequence 455311 from Patent EP1572962.
JD186174 - Sequence 167198 from Patent EP1572962.
JD329590 - Sequence 310614 from Patent EP1572962.
JD346198 - Sequence 327222 from Patent EP1572962.
JD508110 - Sequence 489134 from Patent EP1572962.
JD194183 - Sequence 175207 from Patent EP1572962.
JD296062 - Sequence 277086 from Patent EP1572962.
JD565037 - Sequence 546061 from Patent EP1572962.
JD208362 - Sequence 189386 from Patent EP1572962.
JD436420 - Sequence 417444 from Patent EP1572962.
JD273087 - Sequence 254111 from Patent EP1572962.
JD513644 - Sequence 494668 from Patent EP1572962.
JD057950 - Sequence 38974 from Patent EP1572962.
JD270135 - Sequence 251159 from Patent EP1572962.
JD456233 - Sequence 437257 from Patent EP1572962.
JD119877 - Sequence 100901 from Patent EP1572962.
JD565324 - Sequence 546348 from Patent EP1572962.

-  Other Names for This Gene
  Alternate Gene Symbols: A8K2V1, ENST00000317668.1, ENST00000317668.2, ENST00000317668.3, ENST00000317668.4, ENST00000317668.5, ENST00000317668.6, ENST00000317668.7, NM_144707, PROM2_HUMAN, PROML2, Q2HIX6, Q8N271, Q8NB84, Q8TAE2, uc002sui.1, uc002sui.2, uc002sui.3, uc002sui.4, UNQ2521/PRO6014
UCSC ID: ENST00000317668.8
RefSeq Accession: NM_144707
Protein: Q8N271 (aka PROM2_HUMAN)
CCDS: CCDS2012.1

-  Methods, Credits, and Use Restrictions
  Click here for details on how this gene model was made and data restrictions if any.