Human Gene WFDC5 (ENST00000372789.6) from GENCODE V44
  Description: Homo sapiens WAP four-disulfide core domain 5 (WFDC5), mRNA. (from RefSeq NM_145652)
RefSeq Summary (NM_145652): This gene encodes a member of the WAP-type four-disulfide core (WFDC) domain family. Most WFDC proteins contain only one WFDC domain, and this encoded protein contains two WFDC domains. The WFDC domain, or WAP signature motif, contains eight cysteines forming four disulfide bonds at the core of the protein, and functions as a protease inhibitor. Most WFDC gene members are localized to chromosome 20q12-q13 in two clusters: centromeric and telomeric. This gene belongs to the centromeric cluster. [provided by RefSeq, Jul 2008]. Sequence Note: This RefSeq record was created from transcript and genomic sequence data to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on transcript alignments.
Gencode Transcript: ENST00000372789.6
Gencode Gene: ENSG00000175121.12
Transcript (Including UTRs)
   Position: hg38 chr20:45,109,463-45,115,174 Size: 5,712 Total Exon Count: 4 Strand: -
Coding Region
   Position: hg38 chr20:45,110,009-45,115,083 Size: 5,075 Coding Exon Count: 4 

Page IndexSequence and LinksUniProtKB CommentsPrimersCTDRNA-Seq Expression
RNA StructureProtein StructureOther SpeciesGO AnnotationsmRNA DescriptionsOther Names
Data last updated at UCSC: 2023-08-18 00:09:47

-  Sequence and Links to Tools and Databases
Genomic Sequence (chr20:45,109,463-45,115,174)mRNA (may differ from genome)Protein (123 aa)
Gene SorterGenome BrowserOther Species FASTAGene interactionsTable SchemaAlphaFold
BioGPSEnsemblEntrez GeneExonPrimerGeneCardsHGNC
WikipediaBioGrid CRISPR DB

-  Comments and Description Text from UniProtKB
DESCRIPTION: RecName: Full=WAP four-disulfide core domain protein 5; AltName: Full=Putative protease inhibitor WAP1; AltName: Full=p53-responsive gene 5 protein; Flags: Precursor;
FUNCTION: Putative acid-stable proteinase inhibitor.
SUBCELLULAR LOCATION: Secreted (Potential).
SIMILARITY: Contains 2 WAP domains.

-  Primer design for this transcript

Primer3Plus can design qPCR Primers that straddle exon-exon-junctions, which amplify only cDNA, not genomic DNA.
Click here to load the transcript sequence and exon structure into Primer3Plus

Exonprimer can design one pair of Sanger sequencing primers around every exon, located in non-genic sequence.
Click here to open Exonprimer with this transcript

To design primers for a non-coding sequence, zoom to a region of interest and select from the drop-down menu: View > In External Tools > Primer3

-  Comparative Toxicogenomics Database (CTD)
  The following chemicals interact with this gene

-  RNA-Seq Expression Data from GTEx (53 Tissues, 570 Donors)
  Highest median expression: 174.38 RPKM in Skin - Not Sun Exposed (Suprapubic)
Total median expression: 343.32 RPKM

View in GTEx track of Genome Browser    View at GTEx portal     View GTEx Body Map

-  mRNA Secondary Structure of 3' and 5' UTRs
RegionFold EnergyBasesEnergy/Base
Display As
5' UTR -27.4091-0.301 Picture PostScript Text
3' UTR -128.80546-0.236 Picture PostScript Text

The RNAfold program from the Vienna RNA Package is used to perform the secondary structure predictions and folding calculations. The estimated folding energy is in kcal/mol. The more negative the energy, the more secondary structure the RNA is likely to have.

-  Protein Domain and Structure Information
  InterPro Domains: Graphical view of domain structure
IPR015874 - 4-disulphide_core
IPR008197 - Whey_acidic_protein_4-diS_core

Pfam Domains:
PF00095 - WAP-type (Whey Acidic Protein) 'four-disulfide core'

ModBase Predicted Comparative 3D Structure on Q8TCV5
The pictures above may be empty if there is no ModBase structure for the protein. The ModBase structure frequently covers just a fragment of the protein. You may be asked to log onto ModBase the first time you click on the pictures. It is simplest after logging in to just click on the picture again to get to the specific info on that model.

-  Orthologous Genes in Other Species
  Orthologies between human, mouse, and rat are computed by taking the best BLASTP hit, and filtering out non-syntenic hits. For more distant species reciprocal-best BLASTP hits are used. Note that the absence of an ortholog in the table below may reflect incomplete annotations in the other species rather than a true absence of the orthologous gene.
MouseRatZebrafishD. melanogasterC. elegansS. cerevisiae
Genome BrowserGenome BrowserNo orthologNo orthologNo orthologNo ortholog
Gene Details     
Gene Sorter     
Protein SequenceProtein Sequence    

-  Gene Ontology (GO) Annotations with Structured Vocabulary
  Molecular Function:
GO:0004867 serine-type endopeptidase inhibitor activity
GO:0030414 peptidase inhibitor activity

Biological Process:
GO:0010466 negative regulation of peptidase activity
GO:0010951 negative regulation of endopeptidase activity

Cellular Component:
GO:0005576 extracellular region

-  Descriptions from all associated GenBank mRNAs
  AY038181 - Homo sapiens probable protease inhibitor WAP1 precursor, mRNA, complete cds.
BC039173 - Homo sapiens WAP four-disulfide core domain 5, mRNA (cDNA clone MGC:21441 IMAGE:4755260), complete cds.
AY358822 - Homo sapiens clone DNA119525 WFDC5 (UNQ3036) mRNA, complete cds.
JD429063 - Sequence 410087 from Patent EP1572962.
JD141139 - Sequence 122163 from Patent EP1572962.
JD138675 - Sequence 119699 from Patent EP1572962.
JD240301 - Sequence 221325 from Patent EP1572962.
JD325699 - Sequence 306723 from Patent EP1572962.
JD224712 - Sequence 205736 from Patent EP1572962.
JD471209 - Sequence 452233 from Patent EP1572962.
JD279788 - Sequence 260812 from Patent EP1572962.
JD326553 - Sequence 307577 from Patent EP1572962.
JD368507 - Sequence 349531 from Patent EP1572962.
JD263816 - Sequence 244840 from Patent EP1572962.
JD024112 - Sequence 5136 from Patent EP1572962.
JD270918 - Sequence 251942 from Patent EP1572962.
JD278677 - Sequence 259701 from Patent EP1572962.
JD527961 - Sequence 508985 from Patent EP1572962.
HQ447949 - Synthetic construct Homo sapiens clone IMAGE:100071311; CCSB008052_02 WAP four-disulfide core domain 5 (WFDC5) gene, encodes complete protein.
KJ895638 - Synthetic construct Homo sapiens clone ccsbBroadEn_05032 WFDC5 gene, encodes complete protein.
CU691668 - Synthetic construct Homo sapiens gateway clone IMAGE:100018517 5' read WFDC5 mRNA.
JD393349 - Sequence 374373 from Patent EP1572962.

-  Other Names for This Gene
  Alternate Gene Symbols: ENST00000372789.1, ENST00000372789.2, ENST00000372789.3, ENST00000372789.4, ENST00000372789.5, NM_145652, PRG5, Q5H981, Q6UWE4, Q8TCV5, uc002xne.1, uc002xne.2, uc002xne.3, UNQ3036/PRO9842, WAP1, WFDC5_HUMAN
UCSC ID: ENST00000372789.6
RefSeq Accession: NM_145652
Protein: Q8TCV5 (aka WFDC5_HUMAN or WFD5_HUMAN)

-  Methods, Credits, and Use Restrictions
  Click here for details on how this gene model was made and data restrictions if any.