Human Gene PKNOX1 (ENST00000291547.10) from GENCODE V44
  Description: Homo sapiens PBX/knotted 1 homeobox 1 (PKNOX1), transcript variant 1, mRNA. (from RefSeq NM_004571)
Gencode Transcript: ENST00000291547.10
Gencode Gene: ENSG00000160199.15
Transcript (Including UTRs)
   Position: hg38 chr21:42,974,562-43,033,931 Size: 59,370 Total Exon Count: 11 Strand: +
Coding Region
   Position: hg38 chr21:43,004,382-43,030,101 Size: 25,720 Coding Exon Count: 10 

Page IndexSequence and LinksUniProtKB CommentsPrimersCTDRNA-Seq Expression
Microarray ExpressionRNA StructureProtein StructureOther SpeciesGO AnnotationsmRNA Descriptions
Other NamesMethods
Data last updated at UCSC: 2023-08-18 00:09:47

-  Sequence and Links to Tools and Databases
 
Genomic Sequence (chr21:42,974,562-43,033,931)mRNA (may differ from genome)Protein (436 aa)
Gene SorterGenome BrowserOther Species FASTAGene interactionsTable SchemaAlphaFold
BioGPSEnsemblEntrez GeneExonPrimerGencodeGeneCards
HGNCHPRDLynxMalacardsMGIOMIM
PubMedUniProtKBWikipediaBioGrid CRISPR DB

-  Comments and Description Text from UniProtKB
  ID: Q96I87_HUMAN
DESCRIPTION: SubName: Full=Homeobox-containing protein PKNOX1; SubName: Full=PBX/knotted 1 homeobox 1; SubName: Full=PBX/knotted 1 homeobox 1, isoform CRA_b;
SUBCELLULAR LOCATION: Nucleus (By similarity).

-  Primer design for this transcript
 

Primer3Plus can design qPCR Primers that straddle exon-exon-junctions, which amplify only cDNA, not genomic DNA.
Click here to load the transcript sequence and exon structure into Primer3Plus

Exonprimer can design one pair of Sanger sequencing primers around every exon, located in non-genic sequence.
Click here to open Exonprimer with this transcript

To design primers for a non-coding sequence, zoom to a region of interest and select from the drop-down menu: View > In External Tools > Primer3


-  Comparative Toxicogenomics Database (CTD)
  The following chemicals interact with this gene           more ... click here to view the complete list

-  RNA-Seq Expression Data from GTEx (53 Tissues, 570 Donors)
  Highest median expression: 11.67 RPKM in Testis
Total median expression: 214.77 RPKM



View in GTEx track of Genome Browser    View at GTEx portal     View GTEx Body Map

+  Microarray Expression Data
  Press "+" in the title bar above to open this section.

-  mRNA Secondary Structure of 3' and 5' UTRs
 
RegionFold EnergyBasesEnergy/Base
Display As
5' UTR -51.40159-0.323 Picture PostScript Text
3' UTR -1160.603830-0.303 Picture PostScript Text

The RNAfold program from the Vienna RNA Package is used to perform the secondary structure predictions and folding calculations. The estimated folding energy is in kcal/mol. The more negative the energy, the more secondary structure the RNA is likely to have.

-  Protein Domain and Structure Information
  InterPro Domains: Graphical view of domain structure
IPR008422 - Homeobox_KN_domain
IPR001356 - Homeodomain
IPR009057 - Homeodomain-like

Pfam Domains:
PF05920 - Homeobox KN domain

ModBase Predicted Comparative 3D Structure on Q96I87
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The pictures above may be empty if there is no ModBase structure for the protein. The ModBase structure frequently covers just a fragment of the protein. You may be asked to log onto ModBase the first time you click on the pictures. It is simplest after logging in to just click on the picture again to get to the specific info on that model.

-  Orthologous Genes in Other Species
  Orthologies between human, mouse, and rat are computed by taking the best BLASTP hit, and filtering out non-syntenic hits. For more distant species reciprocal-best BLASTP hits are used. Note that the absence of an ortholog in the table below may reflect incomplete annotations in the other species rather than a true absence of the orthologous gene.
MouseRatZebrafishD. melanogasterC. elegansS. cerevisiae
Genome BrowserGenome BrowserGenome BrowserNo orthologNo orthologNo ortholog
Gene Details     
Gene Sorter     
MGIRGDEnsembl   
Protein SequenceProtein SequenceProtein Sequence   
AlignmentAlignmentAlignment   

-  Gene Ontology (GO) Annotations with Structured Vocabulary
  Molecular Function:
GO:0001228 transcriptional activator activity, RNA polymerase II transcription regulatory region sequence-specific binding
GO:0003677 DNA binding
GO:0003705 transcription factor activity, RNA polymerase II distal enhancer sequence-specific binding
GO:0005515 protein binding
GO:0043565 sequence-specific DNA binding
GO:0046982 protein heterodimerization activity

Biological Process:
GO:0001525 angiogenesis
GO:0006355 regulation of transcription, DNA-templated
GO:0006357 regulation of transcription from RNA polymerase II promoter
GO:0006366 transcription from RNA polymerase II promoter
GO:0030097 hemopoiesis
GO:0030217 T cell differentiation
GO:0030218 erythrocyte differentiation
GO:0043010 camera-type eye development
GO:0045944 positive regulation of transcription from RNA polymerase II promoter

Cellular Component:
GO:0005634 nucleus
GO:0005667 transcription factor complex
GO:0005737 cytoplasm
GO:0005829 cytosol


-  Descriptions from all associated GenBank mRNAs
  AY196965 - Homo sapiens homeobox-containing protein PKNOX1 mRNA, complete cds.
AY142115 - Homo sapiens PKNOX1B (PKNOX1) mRNA, complete cds; alternatively spliced.
BC000735 - Homo sapiens PBX/knotted 1 homeobox 1, mRNA (cDNA clone IMAGE:3504770), partial cds.
Y13613 - Homo sapiens mRNA for homeodomain protein Prep-1.
AK294794 - Homo sapiens cDNA FLJ53736 complete cds, highly similar to Homeobox protein PKNOX1.
AK222892 - Homo sapiens mRNA for PBX/knotted 1 homeobox 1 isoform 1 variant, clone: HRC01728.
U68727 - Human homeobox-containing protein mRNA, complete cds.
AY566249 - Homo sapiens pkonx1c mRNA, complete cds, alternatively spliced.
JD358188 - Sequence 339212 from Patent EP1572962.
BC007746 - Homo sapiens PBX/knotted 1 homeobox 1, mRNA (cDNA clone MGC:12791 IMAGE:4099156), complete cds.
DQ895961 - Synthetic construct Homo sapiens clone IMAGE:100010421; FLH188998.01L; RZPDo839A0764D PBX/knotted 1 homeobox 1 (PKNOX1) gene, encodes complete protein.
DQ892709 - Synthetic construct clone IMAGE:100005339; FLH189002.01X; RZPDo839A0774D PBX/knotted 1 homeobox 1 (PKNOX1) gene, encodes complete protein.
AB384928 - Synthetic construct DNA, clone: pF1KB4325, Homo sapiens PKNOX1 gene for PBX/knotted 1 homeobox 1, complete cds, without stop codon, in Flexi system.
CU675791 - Synthetic construct Homo sapiens gateway clone IMAGE:100019388 5' read PKNOX1 mRNA.
KJ897338 - Synthetic construct Homo sapiens clone ccsbBroadEn_06732 PKNOX1 gene, encodes complete protein.
JD177810 - Sequence 158834 from Patent EP1572962.
JD546654 - Sequence 527678 from Patent EP1572962.
JD210135 - Sequence 191159 from Patent EP1572962.
DQ579308 - Homo sapiens piRNA piR-47420, complete sequence.
JD097737 - Sequence 78761 from Patent EP1572962.
JD141135 - Sequence 122159 from Patent EP1572962.
JD465082 - Sequence 446106 from Patent EP1572962.
JD561614 - Sequence 542638 from Patent EP1572962.
JD556263 - Sequence 537287 from Patent EP1572962.
JD530670 - Sequence 511694 from Patent EP1572962.
JD276674 - Sequence 257698 from Patent EP1572962.
JD316564 - Sequence 297588 from Patent EP1572962.
JD371264 - Sequence 352288 from Patent EP1572962.
JD337556 - Sequence 318580 from Patent EP1572962.
JD247535 - Sequence 228559 from Patent EP1572962.
JD321768 - Sequence 302792 from Patent EP1572962.
JD553360 - Sequence 534384 from Patent EP1572962.
JD242929 - Sequence 223953 from Patent EP1572962.
JD507411 - Sequence 488435 from Patent EP1572962.
JD119918 - Sequence 100942 from Patent EP1572962.
JD374384 - Sequence 355408 from Patent EP1572962.
JD378905 - Sequence 359929 from Patent EP1572962.
JD502162 - Sequence 483186 from Patent EP1572962.
JD087431 - Sequence 68455 from Patent EP1572962.
JD310007 - Sequence 291031 from Patent EP1572962.
JD364274 - Sequence 345298 from Patent EP1572962.
JD217767 - Sequence 198791 from Patent EP1572962.
JD196456 - Sequence 177480 from Patent EP1572962.
JD132483 - Sequence 113507 from Patent EP1572962.
JD348183 - Sequence 329207 from Patent EP1572962.
JD180753 - Sequence 161777 from Patent EP1572962.
JD181599 - Sequence 162623 from Patent EP1572962.
JD162102 - Sequence 143126 from Patent EP1572962.
JD548825 - Sequence 529849 from Patent EP1572962.
JD495140 - Sequence 476164 from Patent EP1572962.
JD530732 - Sequence 511756 from Patent EP1572962.
JD171305 - Sequence 152329 from Patent EP1572962.
JD313676 - Sequence 294700 from Patent EP1572962.
JD349070 - Sequence 330094 from Patent EP1572962.
JD508210 - Sequence 489234 from Patent EP1572962.
JD334526 - Sequence 315550 from Patent EP1572962.
JD530912 - Sequence 511936 from Patent EP1572962.
JD109322 - Sequence 90346 from Patent EP1572962.
JD232990 - Sequence 214014 from Patent EP1572962.
JD117721 - Sequence 98745 from Patent EP1572962.
JD181528 - Sequence 162552 from Patent EP1572962.
JD283817 - Sequence 264841 from Patent EP1572962.
JD427779 - Sequence 408803 from Patent EP1572962.

-  Other Names for This Gene
  Alternate Gene Symbols: ENST00000291547.1, ENST00000291547.2, ENST00000291547.3, ENST00000291547.4, ENST00000291547.5, ENST00000291547.6, ENST00000291547.7, ENST00000291547.8, ENST00000291547.9, hCG_2043122, NM_004571, Q96I87, Q96I87_HUMAN, uc002zcq.1, uc002zcq.2, uc002zcq.3, uc002zcq.4
UCSC ID: ENST00000291547.10
RefSeq Accession: NM_004571
Protein: Q96I87 CCDS: CCDS13692.1

-  Methods, Credits, and Use Restrictions
  Click here for details on how this gene model was made and data restrictions if any.