Human Gene LIF (ENST00000249075.4) from GENCODE V44
  Description: Homo sapiens LIF interleukin 6 family cytokine (LIF), transcript variant 1, mRNA. (from RefSeq NM_002309)
RefSeq Summary (NM_002309): The protein encoded by this gene is a pleiotropic cytokine with roles in several different systems. It is involved in the induction of hematopoietic differentiation in normal and myeloid leukemia cells, induction of neuronal cell differentiation, regulator of mesenchymal to epithelial conversion during kidney development, and may also have a role in immune tolerance at the maternal-fetal interface. Alternatively spliced transcript variants encoding multiple isoforms have been observed for this gene. [provided by RefSeq, Mar 2012].
Gencode Transcript: ENST00000249075.4
Gencode Gene: ENSG00000128342.5
Transcript (Including UTRs)
   Position: hg38 chr22:30,240,453-30,246,759 Size: 6,307 Total Exon Count: 3 Strand: -
Coding Region
   Position: hg38 chr22:30,243,651-30,246,695 Size: 3,045 Coding Exon Count: 3 

Page IndexSequence and LinksUniProtKB CommentsPrimersMalaCardsCTD
RNA-Seq ExpressionMicroarray ExpressionRNA StructureProtein StructureOther SpeciesGO Annotations
mRNA DescriptionsPathwaysOther NamesMethods
Data last updated at UCSC: 2023-08-18 00:09:47

-  Sequence and Links to Tools and Databases
 
Genomic Sequence (chr22:30,240,453-30,246,759)mRNA (may differ from genome)Protein (202 aa)
Gene SorterGenome BrowserOther Species FASTAGene interactionsTable SchemaAlphaFold
BioGPSEnsemblEntrez GeneExonPrimerGencodeGeneCards
HGNCHPRDLynxMalacardsMGIneXtProt
OMIMPubMedReactomeUniProtKBWikipediaBioGrid CRISPR DB

-  Comments and Description Text from UniProtKB
  ID: LIF_HUMAN
DESCRIPTION: RecName: Full=Leukemia inhibitory factor; Short=LIF; AltName: Full=Differentiation-stimulating factor; Short=D factor; AltName: Full=Melanoma-derived LPL inhibitor; Short=MLPLI; AltName: INN=Emfilermin; Flags: Precursor;
FUNCTION: LIF has the capacity to induce terminal differentiation in leukemic cells. Its activities include the induction of hematopoietic differentiation in normal and myeloid leukemia cells, the induction of neuronal cell differentiation, and the stimulation of acute-phase protein synthesis in hepatocytes.
SUBCELLULAR LOCATION: Secreted.
PHARMACEUTICAL: In phase II clinical trial. The drug is being developed by Amrad to assist embryo implantation in women who have failed to become pregnant despite assisted reproductive technologies (ART).
SIMILARITY: Belongs to the LIF/OSM family.
WEB RESOURCE: Name=Wikipedia; Note=Leukemia inhibitory factor entry; URL="http://en.wikipedia.org/wiki/Leukemia_inhibitory_factor";

-  Primer design for this transcript
 

Primer3Plus can design qPCR Primers that straddle exon-exon-junctions, which amplify only cDNA, not genomic DNA.
Click here to load the transcript sequence and exon structure into Primer3Plus

Exonprimer can design one pair of Sanger sequencing primers around every exon, located in non-genic sequence.
Click here to open Exonprimer with this transcript

To design primers for a non-coding sequence, zoom to a region of interest and select from the drop-down menu: View > In External Tools > Primer3


-  MalaCards Disease Associations
  MalaCards Gene Search: LIF
Diseases sorted by gene-association score: leukemia (76), ectopic pregnancy (16), null-cell leukemia (11), complement deficiency (11), stachybotrys chartarum (10), embryonal carcinoma (9), myeloid leukemia (8), skeletal muscle regeneration (8), lesion of sciatic nerve (7), mononeuritis of lower limb (7), pregnancy loss, recurrent 1 (7), somatization disorder (6), breast carcinoma in situ (5), letterer-siwe disease (5), motor neuron disease (2)

-  Comparative Toxicogenomics Database (CTD)
  The following chemicals interact with this gene           more ... click here to view the complete list

-  RNA-Seq Expression Data from GTEx (53 Tissues, 570 Donors)
  Highest median expression: 5.96 RPKM in Adipose - Visceral (Omentum)
Total median expression: 70.75 RPKM



View in GTEx track of Genome Browser    View at GTEx portal     View GTEx Body Map

+  Microarray Expression Data
  Press "+" in the title bar above to open this section.

-  mRNA Secondary Structure of 3' and 5' UTRs
 
RegionFold EnergyBasesEnergy/Base
Display As
5' UTR -13.7064-0.214 Picture PostScript Text
3' UTR -1348.803198-0.422 Picture PostScript Text

The RNAfold program from the Vienna RNA Package is used to perform the secondary structure predictions and folding calculations. The estimated folding energy is in kcal/mol. The more negative the energy, the more secondary structure the RNA is likely to have.

-  Protein Domain and Structure Information
  InterPro Domains: Graphical view of domain structure
IPR009079 - 4_helix_cytokine-like_core
IPR012351 - 4_helix_cytokine_core
IPR003624 - Leukemia_IF
IPR001581 - Leukemia_IF/oncostatin
IPR019827 - Leukemia_IF/oncostatin_CS

Pfam Domains:
PF01291 - LIF / OSM family

Protein Data Bank (PDB) 3-D Structure
MuPIT help
1EMR - X-ray MuPIT 1PVH - X-ray MuPIT 2Q7N - X-ray MuPIT


ModBase Predicted Comparative 3D Structure on P15018
FrontTopSide
The pictures above may be empty if there is no ModBase structure for the protein. The ModBase structure frequently covers just a fragment of the protein. You may be asked to log onto ModBase the first time you click on the pictures. It is simplest after logging in to just click on the picture again to get to the specific info on that model.

-  Orthologous Genes in Other Species
  Orthologies between human, mouse, and rat are computed by taking the best BLASTP hit, and filtering out non-syntenic hits. For more distant species reciprocal-best BLASTP hits are used. Note that the absence of an ortholog in the table below may reflect incomplete annotations in the other species rather than a true absence of the orthologous gene.
MouseRatZebrafishD. melanogasterC. elegansS. cerevisiae
Genome BrowserGenome BrowserNo orthologNo orthologNo orthologNo ortholog
Gene Details     
Gene Sorter     
MGIRGD    
Protein SequenceProtein Sequence    
AlignmentAlignment    

-  Gene Ontology (GO) Annotations with Structured Vocabulary
  Molecular Function:
GO:0005102 receptor binding
GO:0005125 cytokine activity
GO:0005146 leukemia inhibitory factor receptor binding
GO:0005515 protein binding
GO:0008083 growth factor activity

Biological Process:
GO:0001974 blood vessel remodeling
GO:0006955 immune response
GO:0007260 tyrosine phosphorylation of STAT protein
GO:0007275 multicellular organism development
GO:0007566 embryo implantation
GO:0008284 positive regulation of cell proliferation
GO:0008285 negative regulation of cell proliferation
GO:0010469 regulation of receptor activity
GO:0010628 positive regulation of gene expression
GO:0019221 cytokine-mediated signaling pathway
GO:0019827 stem cell population maintenance
GO:0030324 lung development
GO:0033138 positive regulation of peptidyl-serine phosphorylation
GO:0033141 positive regulation of peptidyl-serine phosphorylation of STAT protein
GO:0042531 positive regulation of tyrosine phosphorylation of STAT protein
GO:0043410 positive regulation of MAPK cascade
GO:0045595 regulation of cell differentiation
GO:0045651 positive regulation of macrophage differentiation
GO:0045835 negative regulation of meiotic nuclear division
GO:0045944 positive regulation of transcription from RNA polymerase II promoter
GO:0046697 decidualization
GO:0046888 negative regulation of hormone secretion
GO:0048286 lung alveolus development
GO:0048644 muscle organ morphogenesis
GO:0048666 neuron development
GO:0048711 positive regulation of astrocyte differentiation
GO:0048861 leukemia inhibitory factor signaling pathway
GO:0048863 stem cell differentiation
GO:0050731 positive regulation of peptidyl-tyrosine phosphorylation
GO:0060135 maternal process involved in female pregnancy
GO:0060426 lung vasculature development
GO:0060463 lung lobe morphogenesis
GO:0060707 trophoblast giant cell differentiation
GO:0060708 spongiotrophoblast differentiation
GO:0070373 negative regulation of ERK1 and ERK2 cascade
GO:0072108 positive regulation of mesenchymal to epithelial transition involved in metanephros morphogenesis
GO:0072307 regulation of metanephric nephron tubule epithelial cell differentiation
GO:1900182 positive regulation of protein localization to nucleus
GO:1901676 positive regulation of histone H3-K27 acetylation
GO:1903025 regulation of RNA polymerase II regulatory region sequence-specific DNA binding

Cellular Component:
GO:0005576 extracellular region
GO:0005615 extracellular space
GO:0005829 cytosol


-  Descriptions from all associated GenBank mRNAs
  LF208981 - JP 2014500723-A/16484: Polycomb-Associated Non-Coding RNAs.
MA444558 - JP 2018138019-A/16484: Polycomb-Associated Non-Coding RNAs.
LF213977 - JP 2014500723-A/21480: Polycomb-Associated Non-Coding RNAs.
MA449554 - JP 2018138019-A/21480: Polycomb-Associated Non-Coding RNAs.
X13967 - Human mRNA for leukaemia inhibitory factor (LIF/HILDA).
JD363155 - Sequence 344179 from Patent EP1572962.
JD180512 - Sequence 161536 from Patent EP1572962.
JD186618 - Sequence 167642 from Patent EP1572962.
JD486264 - Sequence 467288 from Patent EP1572962.
JD093881 - Sequence 74905 from Patent EP1572962.
JD133860 - Sequence 114884 from Patent EP1572962.
JD386144 - Sequence 367168 from Patent EP1572962.
JD386145 - Sequence 367169 from Patent EP1572962.
LF324642 - JP 2014500723-A/132145: Polycomb-Associated Non-Coding RNAs.
MA560219 - JP 2018138019-A/132145: Polycomb-Associated Non-Coding RNAs.
JD563158 - Sequence 544182 from Patent EP1572962.
JD476243 - Sequence 457267 from Patent EP1572962.
LF324641 - JP 2014500723-A/132144: Polycomb-Associated Non-Coding RNAs.
MA560218 - JP 2018138019-A/132144: Polycomb-Associated Non-Coding RNAs.
JD459042 - Sequence 440066 from Patent EP1572962.
JD098220 - Sequence 79244 from Patent EP1572962.
JD432379 - Sequence 413403 from Patent EP1572962.
LF324639 - JP 2014500723-A/132142: Polycomb-Associated Non-Coding RNAs.
MA560216 - JP 2018138019-A/132142: Polycomb-Associated Non-Coding RNAs.
JD276967 - Sequence 257991 from Patent EP1572962.
JD383913 - Sequence 364937 from Patent EP1572962.
JD080002 - Sequence 61026 from Patent EP1572962.
JD159818 - Sequence 140842 from Patent EP1572962.
JD456680 - Sequence 437704 from Patent EP1572962.
JD444329 - Sequence 425353 from Patent EP1572962.
JD376655 - Sequence 357679 from Patent EP1572962.
LF324638 - JP 2014500723-A/132141: Polycomb-Associated Non-Coding RNAs.
MA560215 - JP 2018138019-A/132141: Polycomb-Associated Non-Coding RNAs.
JD195424 - Sequence 176448 from Patent EP1572962.
JD449349 - Sequence 430373 from Patent EP1572962.
JD234938 - Sequence 215962 from Patent EP1572962.
JD348713 - Sequence 329737 from Patent EP1572962.
JD219095 - Sequence 200119 from Patent EP1572962.
JD477524 - Sequence 458548 from Patent EP1572962.
JD475453 - Sequence 456477 from Patent EP1572962.
JD158387 - Sequence 139411 from Patent EP1572962.
LF324637 - JP 2014500723-A/132140: Polycomb-Associated Non-Coding RNAs.
MA560214 - JP 2018138019-A/132140: Polycomb-Associated Non-Coding RNAs.
JD306513 - Sequence 287537 from Patent EP1572962.
JD261466 - Sequence 242490 from Patent EP1572962.
LF324636 - JP 2014500723-A/132139: Polycomb-Associated Non-Coding RNAs.
MA560213 - JP 2018138019-A/132139: Polycomb-Associated Non-Coding RNAs.
JD481658 - Sequence 462682 from Patent EP1572962.
JD037169 - Sequence 18193 from Patent EP1572962.
JD518751 - Sequence 499775 from Patent EP1572962.
JD261405 - Sequence 242429 from Patent EP1572962.
JD122826 - Sequence 103850 from Patent EP1572962.
JD391883 - Sequence 372907 from Patent EP1572962.
JD173451 - Sequence 154475 from Patent EP1572962.
JD251905 - Sequence 232929 from Patent EP1572962.
JD464232 - Sequence 445256 from Patent EP1572962.
JD559230 - Sequence 540254 from Patent EP1572962.
LF324634 - JP 2014500723-A/132137: Polycomb-Associated Non-Coding RNAs.
MA560211 - JP 2018138019-A/132137: Polycomb-Associated Non-Coding RNAs.
JD401360 - Sequence 382384 from Patent EP1572962.
JD451776 - Sequence 432800 from Patent EP1572962.
JD354599 - Sequence 335623 from Patent EP1572962.
JD200438 - Sequence 181462 from Patent EP1572962.
JD427709 - Sequence 408733 from Patent EP1572962.
JD521818 - Sequence 502842 from Patent EP1572962.
JD400535 - Sequence 381559 from Patent EP1572962.
JD093178 - Sequence 74202 from Patent EP1572962.
JD537689 - Sequence 518713 from Patent EP1572962.
JD220302 - Sequence 201326 from Patent EP1572962.
JD409202 - Sequence 390226 from Patent EP1572962.
JD481491 - Sequence 462515 from Patent EP1572962.
JD342760 - Sequence 323784 from Patent EP1572962.
JD102545 - Sequence 83569 from Patent EP1572962.
JD384135 - Sequence 365159 from Patent EP1572962.
JD393315 - Sequence 374339 from Patent EP1572962.
JD475860 - Sequence 456884 from Patent EP1572962.
JD371205 - Sequence 352229 from Patent EP1572962.
JD187526 - Sequence 168550 from Patent EP1572962.
JD321226 - Sequence 302250 from Patent EP1572962.
JD410819 - Sequence 391843 from Patent EP1572962.
JD079225 - Sequence 60249 from Patent EP1572962.
JD484497 - Sequence 465521 from Patent EP1572962.
JD234348 - Sequence 215372 from Patent EP1572962.
JD343643 - Sequence 324667 from Patent EP1572962.
JD465784 - Sequence 446808 from Patent EP1572962.
JD288040 - Sequence 269064 from Patent EP1572962.
JD330610 - Sequence 311634 from Patent EP1572962.
JD440504 - Sequence 421528 from Patent EP1572962.
JD353637 - Sequence 334661 from Patent EP1572962.
JD507371 - Sequence 488395 from Patent EP1572962.
JD197305 - Sequence 178329 from Patent EP1572962.
JD429584 - Sequence 410608 from Patent EP1572962.
JD453297 - Sequence 434321 from Patent EP1572962.
JD318480 - Sequence 299504 from Patent EP1572962.
JD098657 - Sequence 79681 from Patent EP1572962.
JD096189 - Sequence 77213 from Patent EP1572962.
JD229691 - Sequence 210715 from Patent EP1572962.
JD290785 - Sequence 271809 from Patent EP1572962.
JD157836 - Sequence 138860 from Patent EP1572962.
JD426012 - Sequence 407036 from Patent EP1572962.
JD384981 - Sequence 366005 from Patent EP1572962.
BC093733 - Homo sapiens leukemia inhibitory factor (cholinergic differentiation factor), mRNA (cDNA clone MGC:120768 IMAGE:7939578), complete cds.
BC093735 - Homo sapiens leukemia inhibitory factor (cholinergic differentiation factor), mRNA (cDNA clone MGC:120770 IMAGE:7939580), complete cds.
BC143305 - Homo sapiens cDNA clone IMAGE:9051810.
BC069540 - Homo sapiens leukemia inhibitory factor (cholinergic differentiation factor), mRNA (cDNA clone MGC:97016 IMAGE:7262225), complete cds.
JD233685 - Sequence 214709 from Patent EP1572962.
AK315310 - Homo sapiens cDNA, FLJ96342, Homo sapiens leukemia inhibitory factor (cholinergicdifferentiation factor) (LIF), mRNA.
KJ904498 - Synthetic construct Homo sapiens clone ccsbBroadEn_13892 LIF-like gene, encodes complete protein.
AB527710 - Synthetic construct DNA, clone: pF1KB6894, Homo sapiens LIF gene for leukemia inhibitory factor, without stop codon, in Flexi system.
LF324632 - JP 2014500723-A/132135: Polycomb-Associated Non-Coding RNAs.
MA560209 - JP 2018138019-A/132135: Polycomb-Associated Non-Coding RNAs.
LF324629 - JP 2014500723-A/132132: Polycomb-Associated Non-Coding RNAs.
MA560206 - JP 2018138019-A/132132: Polycomb-Associated Non-Coding RNAs.
JD235601 - Sequence 216625 from Patent EP1572962.

-  Biochemical and Signaling Pathways
  KEGG - Kyoto Encyclopedia of Genes and Genomes
hsa04060 - Cytokine-cytokine receptor interaction
hsa04630 - Jak-STAT signaling pathway

Reactome (by CSHL, EBI, and GO)

Protein P15018 (Reactome details) participates in the following event(s):

R-HSA-6783681 LIF binds LIFR
R-HSA-6783524 OSM,LIF,CTF1 receptor complex binds gp130
R-HSA-6788467 IL-6-type cytokine receptor ligand interactions
R-HSA-6783783 Interleukin-10 signaling
R-HSA-6785807 Interleukin-4 and 13 signaling
R-HSA-6783589 Interleukin-6 family signaling
R-HSA-449147 Signaling by Interleukins
R-HSA-1280215 Cytokine Signaling in Immune system
R-HSA-168256 Immune System

-  Other Names for This Gene
  Alternate Gene Symbols: B2RCW7, ENST00000249075.1, ENST00000249075.2, ENST00000249075.3, HILDA, LIF_HUMAN, NM_002309, P15018, Q52LZ2, uc003agz.1, uc003agz.2, uc003agz.3, uc003agz.4
UCSC ID: ENST00000249075.4
RefSeq Accession: NM_002309
Protein: P15018 (aka LIF_HUMAN)
CCDS: CCDS13872.1

-  Methods, Credits, and Use Restrictions
  Click here for details on how this gene model was made and data restrictions if any.