Human Gene MPST (ENST00000401419.7) from GENCODE V44
Description: Homo sapiens mercaptopyruvate sulfurtransferase (MPST), transcript variant 2, mRNA. (from RefSeq NM_001013436) RefSeq Summary (NM_001013436): This protein encoded by this gene catalyzes the transfer of a sulfur ion from 3-mercaptopyruvate to cyanide or other thiol compounds. It may be involved in cysteine degradation and cyanide detoxification. There is confusion in literature between this protein (mercaptopyruvate sulfurtransferase, MPST), which appears to be cytoplasmic, and thiosulfate sulfurtransferase (rhodanese, TST, GeneID:7263), which is a mitochondrial protein. Deficiency in MPST activity has been implicated in a rare inheritable disorder known as mercaptolactate-cysteine disulfiduria (MCDU). Alternatively spliced transcript variants encoding same or different isoforms have been identified for this gene. [provided by RefSeq, Jul 2008]. Sequence Note: The RefSeq transcript and protein were derived from genomic sequence to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on alignments. Gencode Transcript: ENST00000401419.7 Gencode Gene: ENSG00000128309.17 Transcript (Including UTRs) Position: hg38 chr22:37,019,635-37,029,801 Size: 10,167 Total Exon Count: 3 Strand: + Coding Region Position: hg38 chr22:37,024,216-37,029,514 Size: 5,299 Coding Exon Count: 2
ID:THTM_HUMAN DESCRIPTION: RecName: Full=3-mercaptopyruvate sulfurtransferase; Short=MST; EC=2.8.1.2; FUNCTION: Transfer of a sulfur ion to cyanide or to other thiol compounds. Also has weak rhodanese activity. May have a role in cyanide degradation or in thiosulfate biosynthesis. CATALYTIC ACTIVITY: 3-mercaptopyruvate + cyanide = pyruvate + thiocyanate. SUBUNIT: Monomer or disulfide-linked homodimer (By similarity). SUBCELLULAR LOCATION: Cytoplasm. DOMAIN: The structure consists of 2 domains of very similar conformation, suggesting a common evolutionary origin. However, the sequences of the 2 domains are very different. SIMILARITY: Contains 2 rhodanese domains. CAUTION: Was originally (PubMed:1953758) thought to be rhodanese.
The RNAfold program from the Vienna RNA Package is used to perform the secondary structure predictions and folding calculations. The estimated folding energy is in kcal/mol. The more negative the energy, the more secondary structure the RNA is likely to have.
ModBase Predicted Comparative 3D Structure on P25325
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Orthologous Genes in Other Species
Orthologies between human, mouse, and rat are computed by taking the best BLASTP hit, and filtering out non-syntenic hits. For more distant species reciprocal-best BLASTP hits are used. Note that the absence of an ortholog in the table below may reflect incomplete annotations in the other species rather than a true absence of the orthologous gene.
KJ904506 - Synthetic construct Homo sapiens clone ccsbBroadEn_13900 MPST gene, encodes complete protein. HM005513 - Homo sapiens clone HTL-T-200 testicular tissue protein Li 200 mRNA, complete cds. BC016737 - Homo sapiens mercaptopyruvate sulfurtransferase, mRNA (cDNA clone MGC:24539 IMAGE:4105509), complete cds. AB208824 - Homo sapiens mRNA for 3-mercaptopyruvate sulfurtransferase variant protein. AK055733 - Homo sapiens cDNA FLJ31171 fis, clone KIDNE2000046, highly similar to 3-mercaptopyruvate sulfurtransferase. BC003508 - Homo sapiens mercaptopyruvate sulfurtransferase, mRNA (cDNA clone MGC:10492 IMAGE:3611253), complete cds. BC009450 - Homo sapiens mercaptopyruvate sulfurtransferase, mRNA (cDNA clone IMAGE:3531108). BC018717 - Homo sapiens mercaptopyruvate sulfurtransferase, mRNA (cDNA clone MGC:31798 IMAGE:4131927), complete cds. X59434 - Human rohu mRNA for rhodanese. CR456523 - Homo sapiens MPST full length open reading frame (ORF) cDNA clone (cDNA clone C22ORF:pGEM.MPST). AB463114 - Synthetic construct DNA, clone: pF1KB3110, Homo sapiens MPST gene for mercaptopyruvate sulfurtransferase, without stop codon, in Flexi system. CU013411 - Homo sapiens MPST, mRNA (cDNA clone IMAGE:100000284), complete cds, without stop codon, in Gateway system. CU013123 - Homo sapiens MPST, mRNA (cDNA clone IMAGE:100000380), complete cds, with stop codon, in Gateway system. CR541712 - Homo sapiens full open reading frame cDNA clone RZPDo834B0129D for gene MPST, mercaptopyruvate sulfurtransferase; complete cds, without stopcodon. BT019636 - Homo sapiens mercaptopyruvate sulfurtransferase mRNA, complete cds. JD059361 - Sequence 40385 from Patent EP1572962. JD421282 - Sequence 402306 from Patent EP1572962. JD053618 - Sequence 34642 from Patent EP1572962. JD551008 - Sequence 532032 from Patent EP1572962. JD200166 - Sequence 181190 from Patent EP1572962. JD477917 - Sequence 458941 from Patent EP1572962.
Biochemical and Signaling Pathways
KEGG - Kyoto Encyclopedia of Genes and Genomes hsa00270 - Cysteine and methionine metabolism hsa01100 - Metabolic pathways
Reactome (by CSHL, EBI, and GO)
Protein P25325 (Reactome details) participates in the following event(s):
R-HSA-9034756 MPST transfers sulfur atom from 3MPYR to form CysS248-MPST R-HSA-9035484 CysS248-MPST:TXN2 dissociates R-HSA-9035227 TXN2 binds CysS248-MPST R-HSA-9012721 MPST transfers sulfur atom from 3MPYR to HSO3- to form S2O3(2-) and PYR R-HSA-9013471 MPST transfers sulfur from 3MPYR to HCN to form HSCN R-HSA-9013533 MPST transfers sulfur from sulfanegen to HCN to form HSCN R-HSA-1614558 Degradation of cysteine and homocysteine R-HSA-1614635 Sulfur amino acid metabolism R-HSA-71291 Metabolism of nitrogenous molecules R-HSA-1430728 Metabolism