Human Gene TIGIT (ENST00000383671.8) from GENCODE V44
  Description: Homo sapiens T cell immunoreceptor with Ig and ITIM domains (TIGIT), mRNA. (from RefSeq NM_173799)
RefSeq Summary (NM_173799): This gene encodes a member of the PVR (poliovirus receptor) family of immunoglobin proteins. The product of this gene is expressed on several classes of T cells including follicular B helper T cells (TFH). The protein has been shown to bind PVR with high affinity; this binding is thought to assist interactions between TFH and dendritic cells to regulate T cell dependent B cell responses.[provided by RefSeq, Sep 2009]. Sequence Note: This RefSeq record was created from transcript and genomic sequence data to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on transcript alignments.
Gencode Transcript: ENST00000383671.8
Gencode Gene: ENSG00000181847.12
Transcript (Including UTRs)
   Position: hg38 chr3:114,294,028-114,310,288 Size: 16,261 Total Exon Count: 4 Strand: +
Coding Region
   Position: hg38 chr3:114,294,062-114,308,131 Size: 14,070 Coding Exon Count: 4 

Page IndexSequence and LinksUniProtKB CommentsPrimersCTDRNA-Seq Expression
Microarray ExpressionRNA StructureProtein StructureOther SpeciesGO AnnotationsmRNA Descriptions
Other NamesMethods
Data last updated at UCSC: 2023-08-18 00:09:47

-  Sequence and Links to Tools and Databases
 
Genomic Sequence (chr3:114,294,028-114,310,288)mRNA (may differ from genome)Protein (244 aa)
Gene SorterGenome BrowserOther Species FASTAGene interactionsTable SchemaAlphaFold
BioGPSEnsemblEntrez GeneExonPrimerGencodeGeneCards
HGNCHPRDLynxMalacardsMGIneXtProt
OMIMPubMedUniProtKBBioGrid CRISPR DB

-  Comments and Description Text from UniProtKB
  ID: TIGIT_HUMAN
DESCRIPTION: RecName: Full=T-cell immunoreceptor with Ig and ITIM domains; AltName: Full=V-set and immunoglobulin domain-containing protein 9; AltName: Full=V-set and transmembrane domain-containing protein 3; Flags: Precursor;
FUNCTION: Binds with high affinity to the poliovirus receptor (PVR) which causes increased secretion of IL10 and decreased secretion of IL12B and suppresses T-cell activation by promoting the generation of mature immunoregulatory dendritic cells.
SUBUNIT: Binds with high affinity to PVR and with lower affinity to PVRL2 and PVRL3.
INTERACTION: Q9NQS3:PVRL3; NbExp=2; IntAct=EBI-4314807, EBI-2826725; Q96NY8:PVRL4; NbExp=2; IntAct=EBI-4314807, EBI-4314784;
SUBCELLULAR LOCATION: Cell membrane; Single-pass type I membrane protein.
TISSUE SPECIFICITY: Expressed at low levels on peripheral memory and regulatory CD4+ T-cells and NK cells and is up-regulated following activation of these cells (at protein level).
DOMAIN: Contains 1 copy of a cytoplasmic motif that is referred to as the immunoreceptor tyrosine-based inhibitor motif (ITIM). This motif is involved in modulation of cellular responses. The phosphorylated ITIM motif can bind the SH2 domain of several SH2- containing phosphatases.
SIMILARITY: Contains 1 Ig-like V-type (immunoglobulin-like) domain.
SEQUENCE CAUTION: Sequence=CAI46183.1; Type=Erroneous initiation;

-  Primer design for this transcript
 

Primer3Plus can design qPCR Primers that straddle exon-exon-junctions, which amplify only cDNA, not genomic DNA.
Click here to load the transcript sequence and exon structure into Primer3Plus

Exonprimer can design one pair of Sanger sequencing primers around every exon, located in non-genic sequence.
Click here to open Exonprimer with this transcript

To design primers for a non-coding sequence, zoom to a region of interest and select from the drop-down menu: View > In External Tools > Primer3


-  Comparative Toxicogenomics Database (CTD)
  The following chemicals interact with this gene

-  RNA-Seq Expression Data from GTEx (53 Tissues, 570 Donors)
  Highest median expression: 3.22 RPKM in Spleen
Total median expression: 11.04 RPKM



View in GTEx track of Genome Browser    View at GTEx portal     View GTEx Body Map

+  Microarray Expression Data
  Press "+" in the title bar above to open this section.

-  mRNA Secondary Structure of 3' and 5' UTRs
 
RegionFold EnergyBasesEnergy/Base
Display As
5' UTR -9.5034-0.279 Picture PostScript Text
3' UTR -624.572157-0.290 Picture PostScript Text

The RNAfold program from the Vienna RNA Package is used to perform the secondary structure predictions and folding calculations. The estimated folding energy is in kcal/mol. The more negative the energy, the more secondary structure the RNA is likely to have.

-  Protein Domain and Structure Information
  InterPro Domains: Graphical view of domain structure
IPR007110 - Ig-like
IPR013783 - Ig-like_fold
IPR003599 - Ig_sub
IPR013106 - Ig_V-set

Pfam Domains:
PF07686 - Immunoglobulin V-set domain

Protein Data Bank (PDB) 3-D Structure
MuPIT help
3Q0H - X-ray MuPIT 3RQ3 - X-ray MuPIT 3UCR - X-ray MuPIT 3UDW - X-ray MuPIT


ModBase Predicted Comparative 3D Structure on Q495A1
FrontTopSide
The pictures above may be empty if there is no ModBase structure for the protein. The ModBase structure frequently covers just a fragment of the protein. You may be asked to log onto ModBase the first time you click on the pictures. It is simplest after logging in to just click on the picture again to get to the specific info on that model.

-  Orthologous Genes in Other Species
  Orthologies between human, mouse, and rat are computed by taking the best BLASTP hit, and filtering out non-syntenic hits. For more distant species reciprocal-best BLASTP hits are used. Note that the absence of an ortholog in the table below may reflect incomplete annotations in the other species rather than a true absence of the orthologous gene.
MouseRatZebrafishD. melanogasterC. elegansS. cerevisiae
Genome BrowserGenome BrowserNo orthologNo orthologNo orthologNo ortholog
Gene Details     
Gene Sorter     
MGI     
Protein SequenceProtein Sequence    
AlignmentAlignment    

-  Gene Ontology (GO) Annotations with Structured Vocabulary
  Molecular Function:
GO:0005102 receptor binding
GO:0005515 protein binding
GO:0042802 identical protein binding
GO:0042803 protein homodimerization activity
GO:0050839 cell adhesion molecule binding

Biological Process:
GO:0007156 homophilic cell adhesion via plasma membrane adhesion molecules
GO:0007157 heterophilic cell-cell adhesion via plasma membrane cell adhesion molecules
GO:0008037 cell recognition
GO:0032695 negative regulation of interleukin-12 production
GO:0032733 positive regulation of interleukin-10 production
GO:0050868 negative regulation of T cell activation

Cellular Component:
GO:0005886 plasma membrane
GO:0005887 integral component of plasma membrane
GO:0005913 cell-cell adherens junction
GO:0009986 cell surface
GO:0016020 membrane
GO:0016021 integral component of membrane


-  Descriptions from all associated GenBank mRNAs
  AL833175 - Homo sapiens mRNA; cDNA DKFZp667A205 (from clone DKFZp667A205).
EU675310 - Homo sapiens T cell immunoreceptor with Ig and ITIM domains (TIGIT) mRNA, complete cds.
LQ882890 - Sequence 39 from Patent WO2018160841.
LQ927250 - Sequence 27 from Patent WO2018191660.
AK097192 - Homo sapiens cDNA FLJ39873 fis, clone SPLEN2015788.
BC101288 - Homo sapiens T cell immunoreceptor with Ig and ITIM domains, mRNA (cDNA clone MGC:120349 IMAGE:40024492), complete cds.
BC101289 - Homo sapiens T cell immunoreceptor with Ig and ITIM domains, mRNA (cDNA clone MGC:120350 IMAGE:40024494), complete cds.
BC101290 - Homo sapiens T cell immunoreceptor with Ig and ITIM domains, mRNA (cDNA clone MGC:120351 IMAGE:40024496), complete cds.
BC101291 - Homo sapiens T cell immunoreceptor with Ig and ITIM domains, mRNA (cDNA clone MGC:120352 IMAGE:40024497), complete cds.
KJ895805 - Synthetic construct Homo sapiens clone ccsbBroadEn_05199 TIGIT gene, encodes complete protein.
KJ900425 - Synthetic construct Homo sapiens clone ccsbBroadEn_09819 TIGIT gene, encodes complete protein.
MP584540 - Sequence 39 from Patent WO2020081767.
JD052495 - Sequence 33519 from Patent EP1572962.
JD102650 - Sequence 83674 from Patent EP1572962.
BX640915 - Homo sapiens mRNA; cDNA DKFZp667N0215 (from clone DKFZp667N0215).
JD187751 - Sequence 168775 from Patent EP1572962.
JD539302 - Sequence 520326 from Patent EP1572962.
JD313071 - Sequence 294095 from Patent EP1572962.
JD555460 - Sequence 536484 from Patent EP1572962.
JD490957 - Sequence 471981 from Patent EP1572962.
JD166680 - Sequence 147704 from Patent EP1572962.
JD263318 - Sequence 244342 from Patent EP1572962.
JD081963 - Sequence 62987 from Patent EP1572962.
JD429729 - Sequence 410753 from Patent EP1572962.
JD526938 - Sequence 507962 from Patent EP1572962.
JD244759 - Sequence 225783 from Patent EP1572962.
JD551590 - Sequence 532614 from Patent EP1572962.
JD142430 - Sequence 123454 from Patent EP1572962.
JD476428 - Sequence 457452 from Patent EP1572962.
JD483661 - Sequence 464685 from Patent EP1572962.
JD037161 - Sequence 18185 from Patent EP1572962.
JD563844 - Sequence 544868 from Patent EP1572962.
JD450373 - Sequence 431397 from Patent EP1572962.

-  Other Names for This Gene
  Alternate Gene Symbols: ENST00000383671.1, ENST00000383671.2, ENST00000383671.3, ENST00000383671.4, ENST00000383671.5, ENST00000383671.6, ENST00000383671.7, NM_173799, Q495A1, Q495A3, Q5JPD8, Q6MZS2, Q8N877, TIGIT_HUMAN, uc003ebg.1, uc003ebg.2, uc003ebg.3, uc003ebg.4, VSIG9, VSTM3
UCSC ID: ENST00000383671.8
RefSeq Accession: NM_173799
Protein: Q495A1 (aka TIGIT_HUMAN)
CCDS: CCDS2980.1

-  Methods, Credits, and Use Restrictions
  Click here for details on how this gene model was made and data restrictions if any.