ID:EAF2_HUMAN DESCRIPTION: RecName: Full=ELL-associated factor 2; AltName: Full=Testosterone-regulated apoptosis inducer and tumor suppressor protein; FUNCTION: Acts as a transcriptional transactivator of TCEA1 elongation activity (By similarity). Acts as a transcriptional transactivator of ELL and ELL2 elongation activities. Potent inducer of apoptosis in prostatic and non-prostatic cell lines. Inhibits prostate tumor growth in vivo. SUBUNIT: Isoform 1 and isoform 2 interact with TCEA1 (By similarity). Interacts with ELL and ELL2. INTERACTION: P0C1Z6:TFPT; NbExp=4; IntAct=EBI-1245604, EBI-1245626; SUBCELLULAR LOCATION: Nucleus speckle. Note=Colocalizes with ELL to nuclear speckles. TISSUE SPECIFICITY: Expressed in heart, brain, placenta, lung, skeletal muscle, kidney, pancreas, spleen, prostate, testis, small intestine, colon, adrenal, bone marrow, lymph node, spinal gland, stomach, thyroid, trachea, thymus, liver and leukocytes. INDUCTION: By androgen. SIMILARITY: Belongs to the EAF family.
The RNAfold program from the Vienna RNA Package is used to perform the secondary structure predictions and folding calculations. The estimated folding energy is in kcal/mol. The more negative the energy, the more secondary structure the RNA is likely to have.
Pfam Domains: PF09816 - RNA polymerase II transcription elongation factor
ModBase Predicted Comparative 3D Structure on Q96CJ1
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Orthologous Genes in Other Species
Orthologies between human, mouse, and rat are computed by taking the best BLASTP hit, and filtering out non-syntenic hits. For more distant species reciprocal-best BLASTP hits are used. Note that the absence of an ortholog in the table below may reflect incomplete annotations in the other species rather than a true absence of the orthologous gene.
Gene Ontology (GO) Annotations with Structured Vocabulary
Molecular Function: GO:0000977 RNA polymerase II regulatory region sequence-specific DNA binding GO:0001228 transcriptional activator activity, RNA polymerase II transcription regulatory region sequence-specific binding GO:0005515 protein binding
Biological Process: GO:0006351 transcription, DNA-templated GO:0006355 regulation of transcription, DNA-templated GO:0006366 transcription from RNA polymerase II promoter GO:0006368 transcription elongation from RNA polymerase II promoter GO:0006915 apoptotic process GO:0030308 negative regulation of cell growth GO:0045893 positive regulation of transcription, DNA-templated GO:0045944 positive regulation of transcription from RNA polymerase II promoter GO:0060770 negative regulation of epithelial cell proliferation involved in prostate gland development
AF217516 - Homo sapiens uncharacterized bone marrow protein BM040 mRNA, complete cds. BC014589 - Homo sapiens ELL associated factor 2, mRNA (cDNA clone IMAGE:4824091). AY049020 - Homo sapiens testosterone regulated apoptosis inducer and tumor suppressor (TRAITS) mRNA, complete cds. JD213611 - Sequence 194635 from Patent EP1572962. BC014209 - Homo sapiens ELL associated factor 2, mRNA (cDNA clone MGC:20630 IMAGE:4765823), complete cds. AF517829 - Homo sapiens EAF2 mRNA, complete cds. AK301560 - Homo sapiens cDNA FLJ60185 complete cds, highly similar to ELL-associated factor 2. AB528527 - Synthetic construct DNA, clone: pF1KB6317, Homo sapiens EAF2 gene for ELL associated factor 2, without stop codon, in Flexi system. CU676923 - Synthetic construct Homo sapiens gateway clone IMAGE:100020652 5' read EAF2 mRNA. DQ892648 - Synthetic construct clone IMAGE:100005278; FLH188622.01X; RZPDo839E0473D ELL associated factor 2 (EAF2) gene, encodes complete protein. DQ895887 - Synthetic construct Homo sapiens clone IMAGE:100010347; FLH188618.01L; RZPDo839E0463D ELL associated factor 2 (EAF2) gene, encodes complete protein. JD047242 - Sequence 28266 from Patent EP1572962.
Biochemical and Signaling Pathways
Reactome (by CSHL, EBI, and GO)
Protein Q96CJ1 (Reactome details) participates in the following event(s):
R-HSA-112381 Hyperphosphorylation (Ser2) of RNA Pol II CTD by P-TEFb complex R-HSA-113429 Elongating transcript encounters a lesion in the template R-HSA-112379 Recruitment of elongation factors to form elongation complex R-HSA-113411 2-4 nt.backtracking of Pol II complex on the template leading to elongation pausing R-HSA-113412 Pol II elongation complex moves on the template as transcript elongates R-HSA-113414 7-14 nt. Backtracking of Pol II complex on the template leading to elongation arrest R-HSA-112385 Addition of nucleotides leads to transcript elongation R-HSA-112392 Resumption of elongation after recovery from pausing R-HSA-113413 TFIIS-mediated recovery of elongation from arrest R-HSA-112395 Abortive termination of elongation after arrest R-HSA-112396 Separation of elongating transcript from template R-HSA-112382 Formation of RNA Pol II elongation complex R-HSA-674695 RNA Polymerase II Pre-transcription Events R-HSA-75955 RNA Polymerase II Transcription Elongation R-HSA-73857 RNA Polymerase II Transcription R-HSA-74160 Gene expression (Transcription)
US Server
