Human Gene PDIA5 (ENST00000316218.12) from GENCODE V44
  Description: Homo sapiens protein disulfide isomerase family A member 5 (PDIA5), transcript variant 1, mRNA. (from RefSeq NM_006810)
RefSeq Summary (NM_006810): This gene encodes a member of the disulfide isomerase (PDI) family of endoplasmic reticulum (ER) proteins that catalyze protein folding and thiol-disulfide interchange reactions. The encoded protein has an N-terminal ER-signal sequence, three catalytically active thioredoxin (TRX) domains, a TRX-like domain, and a C-terminal ER-retention sequence. The N-terminal TRX-like domain is the primary binding site for the major ER chaperone calreticulin and possibly other proteins and substrates as well. Alternative splicing results in multiple protein- and non-protein-coding transcript variants. [provided by RefSeq, Dec 2016].
Gencode Transcript: ENST00000316218.12
Gencode Gene: ENSG00000065485.20
Transcript (Including UTRs)
   Position: hg38 chr3:123,067,025-123,162,104 Size: 95,080 Total Exon Count: 17 Strand: +
Coding Region
   Position: hg38 chr3:123,067,165-123,161,960 Size: 94,796 Coding Exon Count: 17 

Page IndexSequence and LinksUniProtKB CommentsPrimersCTDRNA-Seq Expression
Microarray ExpressionRNA StructureProtein StructureOther SpeciesGO AnnotationsmRNA Descriptions
PathwaysOther NamesMethods
Data last updated at UCSC: 2023-08-18 00:09:47

-  Sequence and Links to Tools and Databases
 
Genomic Sequence (chr3:123,067,025-123,162,104)mRNA (may differ from genome)Protein (519 aa)
Gene SorterGenome BrowserOther Species FASTAGene interactionsTable SchemaAlphaFold
BioGPSEnsemblEntrez GeneExonPrimerGencodeGeneCards
HGNCHPRDLynxMGIneXtProtOMIM
PubMedReactomeUniProtKBBioGrid CRISPR DB

-  Comments and Description Text from UniProtKB
  ID: PDIA5_HUMAN
DESCRIPTION: RecName: Full=Protein disulfide-isomerase A5; EC=5.3.4.1; AltName: Full=Protein disulfide isomerase-related protein; Flags: Precursor;
CATALYTIC ACTIVITY: Catalyzes the rearrangement of -S-S- bonds in proteins.
SUBCELLULAR LOCATION: Endoplasmic reticulum lumen (By similarity).
SIMILARITY: Belongs to the protein disulfide isomerase family.
SIMILARITY: Contains 3 thioredoxin domains.

-  Primer design for this transcript
 

Primer3Plus can design qPCR Primers that straddle exon-exon-junctions, which amplify only cDNA, not genomic DNA.
Click here to load the transcript sequence and exon structure into Primer3Plus

Exonprimer can design one pair of Sanger sequencing primers around every exon, located in non-genic sequence.
Click here to open Exonprimer with this transcript

To design primers for a non-coding sequence, zoom to a region of interest and select from the drop-down menu: View > In External Tools > Primer3


-  Comparative Toxicogenomics Database (CTD)
  The following chemicals interact with this gene           more ... click here to view the complete list

-  RNA-Seq Expression Data from GTEx (53 Tissues, 570 Donors)
  Highest median expression: 17.88 RPKM in Liver
Total median expression: 288.16 RPKM



View in GTEx track of Genome Browser    View at GTEx portal     View GTEx Body Map

+  Microarray Expression Data
  Press "+" in the title bar above to open this section.

-  mRNA Secondary Structure of 3' and 5' UTRs
 
RegionFold EnergyBasesEnergy/Base
Display As
5' UTR -58.80140-0.420 Picture PostScript Text
3' UTR -13.90144-0.097 Picture PostScript Text

The RNAfold program from the Vienna RNA Package is used to perform the secondary structure predictions and folding calculations. The estimated folding energy is in kcal/mol. The more negative the energy, the more secondary structure the RNA is likely to have.

-  Protein Domain and Structure Information
  InterPro Domains: Graphical view of domain structure
IPR005746 - Thioredoxin
IPR012336 - Thioredoxin-like_fold
IPR017937 - Thioredoxin_CS
IPR013766 - Thioredoxin_domain

Pfam Domains:
PF00085 - Thioredoxin

ModBase Predicted Comparative 3D Structure on Q14554
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-  Orthologous Genes in Other Species
  Orthologies between human, mouse, and rat are computed by taking the best BLASTP hit, and filtering out non-syntenic hits. For more distant species reciprocal-best BLASTP hits are used. Note that the absence of an ortholog in the table below may reflect incomplete annotations in the other species rather than a true absence of the orthologous gene.
MouseRatZebrafishD. melanogasterC. elegansS. cerevisiae
Genome BrowserGenome BrowserGenome BrowserGenome BrowserNo orthologNo ortholog
Gene Details     
Gene Sorter     
MGIRGDEnsemblEnsembl  
Protein SequenceProtein SequenceProtein SequenceProtein Sequence  
AlignmentAlignmentAlignmentAlignment  

-  Gene Ontology (GO) Annotations with Structured Vocabulary
  Molecular Function:
GO:0003756 protein disulfide isomerase activity
GO:0005515 protein binding
GO:0015037 peptide disulfide oxidoreductase activity
GO:0016491 oxidoreductase activity
GO:0016853 isomerase activity

Biological Process:
GO:0006457 protein folding
GO:0034976 response to endoplasmic reticulum stress
GO:0036498 IRE1-mediated unfolded protein response
GO:0045454 cell redox homeostasis
GO:0055114 oxidation-reduction process

Cellular Component:
GO:0005783 endoplasmic reticulum
GO:0005788 endoplasmic reticulum lumen
GO:0005789 endoplasmic reticulum membrane


-  Descriptions from all associated GenBank mRNAs
  BC001625 - Homo sapiens protein disulfide isomerase family A, member 5, mRNA (cDNA clone MGC:1259 IMAGE:3537659), complete cds.
D49490 - Homo sapiens mRNA for protein disulfide isomerase-related protein (PDIR), complete cds.
KJ900838 - Synthetic construct Homo sapiens clone ccsbBroadEn_10232 PDIA5 gene, encodes complete protein.
JD217424 - Sequence 198448 from Patent EP1572962.
JD141807 - Sequence 122831 from Patent EP1572962.
JD339785 - Sequence 320809 from Patent EP1572962.
AK054963 - Homo sapiens cDNA FLJ30401 fis, clone BRACE2008443, highly similar to PROTEIN DISULFIDE ISOMERASE-RELATED PROTEIN PRECURSOR (EC 5.3.4.1).
JD554405 - Sequence 535429 from Patent EP1572962.
JD162108 - Sequence 143132 from Patent EP1572962.

-  Biochemical and Signaling Pathways
  Reactome (by CSHL, EBI, and GO)

Protein Q14554 (Reactome details) participates in the following event(s):

R-HSA-381038 XBP1(S) activates chaperone genes
R-HSA-381070 IRE1alpha activates chaperones
R-HSA-381119 Unfolded Protein Response (UPR)
R-HSA-392499 Metabolism of proteins

-  Other Names for This Gene
  Alternate Gene Symbols: D3DN95, ENST00000316218.1, ENST00000316218.10, ENST00000316218.11, ENST00000316218.2, ENST00000316218.3, ENST00000316218.4, ENST00000316218.5, ENST00000316218.6, ENST00000316218.7, ENST00000316218.8, ENST00000316218.9, NM_006810, PDIA5_HUMAN, PDIR, Q14554, uc003egc.1, uc003egc.2, uc003egc.3, uc003egc.4
UCSC ID: ENST00000316218.12
RefSeq Accession: NM_006810
Protein: Q14554 (aka PDIA5_HUMAN)
CCDS: CCDS3020.1

-  Methods, Credits, and Use Restrictions
  Click here for details on how this gene model was made and data restrictions if any.