Human Gene ADH4 (ENST00000265512.12) from GENCODE V44
  Description: Homo sapiens alcohol dehydrogenase 4 (class II), pi polypeptide (ADH4), transcript variant 3, mRNA. (from RefSeq NM_000670)
RefSeq Summary (NM_000670): This gene encodes class II alcohol dehydrogenase 4 pi subunit, which is a member of the alcohol dehydrogenase family. Members of this enzyme family metabolize a wide variety of substrates, including ethanol, retinol, other aliphatic alcohols, hydroxysteroids, and lipid peroxidation products. Class II alcohol dehydrogenase is a homodimer composed of 2 pi subunits. It exhibits a high activity for oxidation of long-chain aliphatic alcohols and aromatic alcohols and is less sensitive to pyrazole. This gene is localized to chromosome 4 in the cluster of alcohol dehydrogenase genes. [provided by RefSeq, Jul 2008].
Gencode Transcript: ENST00000265512.12
Gencode Gene: ENSG00000198099.10
Transcript (Including UTRs)
   Position: hg38 chr4:99,123,658-99,144,297 Size: 20,640 Total Exon Count: 9 Strand: -
Coding Region
   Position: hg38 chr4:99,124,442-99,144,222 Size: 19,781 Coding Exon Count: 9 

Page IndexSequence and LinksUniProtKB CommentsPrimersMalaCardsCTD
RNA-Seq ExpressionRNA StructureProtein StructureOther SpeciesGO AnnotationsmRNA Descriptions
PathwaysOther NamesMethods
Data last updated at UCSC: 2023-08-18 00:09:47

-  Sequence and Links to Tools and Databases
 
Genomic Sequence (chr4:99,123,658-99,144,297)mRNA (may differ from genome)Protein (380 aa)
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OMIMPubMedReactomeUniProtKBWikipediaBioGrid CRISPR DB

-  Comments and Description Text from UniProtKB
  ID: ADH4_HUMAN
DESCRIPTION: RecName: Full=Alcohol dehydrogenase 4; EC=1.1.1.1; AltName: Full=Alcohol dehydrogenase class II pi chain;
CATALYTIC ACTIVITY: An alcohol + NAD(+) = an aldehyde or ketone + NADH.
COFACTOR: Binds 2 zinc ions per subunit.
SUBUNIT: Homodimer.
SUBCELLULAR LOCATION: Cytoplasm.
MISCELLANEOUS: There are 7 different ADH's isozymes in human: three belongs to class-I: alpha, beta, and gamma, one to class-II: pi, one to class-III: chi, one to class-IV: ADH7 and one to class- V: ADH6.
SIMILARITY: Belongs to the zinc-containing alcohol dehydrogenase family. Class-II subfamily.
WEB RESOURCE: Name=NIEHS-SNPs; URL="http://egp.gs.washington.edu/data/adh4/";

-  Primer design for this transcript
 

Primer3Plus can design qPCR Primers that straddle exon-exon-junctions, which amplify only cDNA, not genomic DNA.
Click here to load the transcript sequence and exon structure into Primer3Plus

Exonprimer can design one pair of Sanger sequencing primers around every exon, located in non-genic sequence.
Click here to open Exonprimer with this transcript

To design primers for a non-coding sequence, zoom to a region of interest and select from the drop-down menu: View > In External Tools > Primer3


-  MalaCards Disease Associations
  MalaCards Gene Search: ADH4
Diseases sorted by gene-association score: alcohol dependence (10), substance dependence (10), hypocalciuric hypercalcemia, type ii (8), alcoholic neuropathy (8), alcoholic liver cirrhosis (7), inflammatory and toxic neuropathy (6)

-  Comparative Toxicogenomics Database (CTD)
  The following chemicals interact with this gene           more ... click here to view the complete list

-  RNA-Seq Expression Data from GTEx (53 Tissues, 570 Donors)
  Highest median expression: 320.45 RPKM in Liver
Total median expression: 332.81 RPKM



View in GTEx track of Genome Browser    View at GTEx portal     View GTEx Body Map

-  mRNA Secondary Structure of 3' and 5' UTRs
 
RegionFold EnergyBasesEnergy/Base
Display As
5' UTR -14.9075-0.199 Picture PostScript Text
3' UTR -173.20784-0.221 Picture PostScript Text

The RNAfold program from the Vienna RNA Package is used to perform the secondary structure predictions and folding calculations. The estimated folding energy is in kcal/mol. The more negative the energy, the more secondary structure the RNA is likely to have.

-  Protein Domain and Structure Information
  InterPro Domains: Graphical view of domain structure
IPR013149 - ADH_C
IPR013154 - ADH_GroES-like
IPR002085 - ADH_SF_Zn-type
IPR002328 - ADH_Zn_CS
IPR011032 - GroES-like

Pfam Domains:
PF08240 - Alcohol dehydrogenase GroES-like domain
PF00107 - Zinc-binding dehydrogenase

Protein Data Bank (PDB) 3-D Structure
MuPIT help
3COS - X-ray MuPIT


ModBase Predicted Comparative 3D Structure on P08319
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The pictures above may be empty if there is no ModBase structure for the protein. The ModBase structure frequently covers just a fragment of the protein. You may be asked to log onto ModBase the first time you click on the pictures. It is simplest after logging in to just click on the picture again to get to the specific info on that model.

-  Orthologous Genes in Other Species
  Orthologies between human, mouse, and rat are computed by taking the best BLASTP hit, and filtering out non-syntenic hits. For more distant species reciprocal-best BLASTP hits are used. Note that the absence of an ortholog in the table below may reflect incomplete annotations in the other species rather than a true absence of the orthologous gene.
MouseRatZebrafishD. melanogasterC. elegansS. cerevisiae
Genome BrowserGenome BrowserNo orthologNo orthologNo orthologNo ortholog
Gene Details     
Gene Sorter     
MGIRGD    
Protein SequenceProtein Sequence    
AlignmentAlignment    

-  Gene Ontology (GO) Annotations with Structured Vocabulary
  Molecular Function:
GO:0003960 NADPH:quinone reductase activity
GO:0004022 alcohol dehydrogenase (NAD) activity
GO:0004024 alcohol dehydrogenase activity, zinc-dependent
GO:0004032 alditol:NADP+ 1-oxidoreductase activity
GO:0004745 retinol dehydrogenase activity
GO:0005503 all-trans retinal binding
GO:0008270 zinc ion binding
GO:0016491 oxidoreductase activity
GO:0016620 oxidoreductase activity, acting on the aldehyde or oxo group of donors, NAD or NADP as acceptor
GO:0019115 benzaldehyde dehydrogenase activity
GO:0019841 retinol binding
GO:0035276 ethanol binding
GO:0046872 metal ion binding
GO:0051287 NAD binding

Biological Process:
GO:0001523 retinoid metabolic process
GO:0006066 alcohol metabolic process
GO:0006067 ethanol metabolic process
GO:0006069 ethanol oxidation
GO:0006081 cellular aldehyde metabolic process
GO:0042572 retinol metabolic process
GO:0046164 alcohol catabolic process
GO:0055114 oxidation-reduction process
GO:1901661 quinone metabolic process

Cellular Component:
GO:0005634 nucleus
GO:0005737 cytoplasm
GO:0005829 cytosol


-  Descriptions from all associated GenBank mRNAs
  AK295556 - Homo sapiens cDNA FLJ55686 complete cds, highly similar to Alcohol dehydrogenase 4 (EC 1.1.1.1).
JD565931 - Sequence 546955 from Patent EP1572962.
EU668317 - Homo sapiens epididymis secretory protein Li 4 (HEL-S-4) mRNA, complete cds.
M15943 - Human class II alchohol dehydrogenase (ADH4) pi subunit mRNA, complete cds.
JD089732 - Sequence 70756 from Patent EP1572962.
JD089733 - Sequence 70757 from Patent EP1572962.
JD243987 - Sequence 225011 from Patent EP1572962.
JD473645 - Sequence 454669 from Patent EP1572962.
JD486289 - Sequence 467313 from Patent EP1572962.
JD473646 - Sequence 454670 from Patent EP1572962.
JD086688 - Sequence 67712 from Patent EP1572962.
JD449239 - Sequence 430263 from Patent EP1572962.
JD110242 - Sequence 91266 from Patent EP1572962.
JD449550 - Sequence 430574 from Patent EP1572962.
JD274173 - Sequence 255197 from Patent EP1572962.
JD355339 - Sequence 336363 from Patent EP1572962.
JD343350 - Sequence 324374 from Patent EP1572962.
JD217245 - Sequence 198269 from Patent EP1572962.
JD363815 - Sequence 344839 from Patent EP1572962.
JD273928 - Sequence 254952 from Patent EP1572962.
BC022319 - Homo sapiens alcohol dehydrogenase 4 (class II), pi polypeptide, mRNA (cDNA clone MGC:22633 IMAGE:4722568), complete cds.
JD500889 - Sequence 481913 from Patent EP1572962.
AK290835 - Homo sapiens cDNA FLJ77091 complete cds, highly similar to Homo sapiens alcohol dehydrogenase 4 (class II), pi polypeptide (ADH4), mRNA.
AK313649 - Homo sapiens cDNA, FLJ94226.
HQ447691 - Synthetic construct Homo sapiens clone IMAGE:100071019; CCSB013053_01 alcohol dehydrogenase 4 (class II), pi polypeptide (ADH4) gene, encodes complete protein.
KJ896384 - Synthetic construct Homo sapiens clone ccsbBroadEn_05778 ADH4 gene, encodes complete protein.
CU692552 - Synthetic construct Homo sapiens gateway clone IMAGE:100022271 5' read ADH4 mRNA.
JD247936 - Sequence 228960 from Patent EP1572962.
JD552759 - Sequence 533783 from Patent EP1572962.
JD337893 - Sequence 318917 from Patent EP1572962.

-  Biochemical and Signaling Pathways
  KEGG - Kyoto Encyclopedia of Genes and Genomes
hsa00010 - Glycolysis / Gluconeogenesis
hsa00071 - Fatty acid metabolism
hsa00350 - Tyrosine metabolism
hsa00830 - Retinol metabolism
hsa00980 - Metabolism of xenobiotics by cytochrome P450
hsa00982 - Drug metabolism - cytochrome P450
hsa01100 - Metabolic pathways

BioCyc Knowledge Library
PWY-6342 - noradrenaline and adrenaline degradation

Reactome (by CSHL, EBI, and GO)

Protein P08319 (Reactome details) participates in the following event(s):

R-HSA-71707 ethanol + NAD+ => acetaldehyde + NADH + H+
R-HSA-5362564 ADH1A,1C,4 oxidise atROL to atRAL in vitro
R-HSA-71384 Ethanol oxidation
R-HSA-5365859 RA biosynthesis pathway
R-HSA-211945 Phase I - Functionalization of compounds
R-HSA-5362517 Signaling by Retinoic Acid
R-HSA-211859 Biological oxidations
R-HSA-9006931 Signaling by Nuclear Receptors
R-HSA-1430728 Metabolism
R-HSA-162582 Signal Transduction

-  Other Names for This Gene
  Alternate Gene Symbols: A8K470, ADH4_HUMAN, B4DIE7, C9J4A9, ENST00000265512.1, ENST00000265512.10, ENST00000265512.11, ENST00000265512.2, ENST00000265512.3, ENST00000265512.4, ENST00000265512.5, ENST00000265512.6, ENST00000265512.7, ENST00000265512.8, ENST00000265512.9, NM_000670, P08319, Q8TCD7, uc003hun.1, uc003hun.2, uc003hun.3, uc003hun.4, uc003hun.5
UCSC ID: ENST00000265512.12
RefSeq Accession: NM_000670
Protein: P08319 (aka ADH4_HUMAN)
CCDS: CCDS34032.1

-  Methods, Credits, and Use Restrictions
  Click here for details on how this gene model was made and data restrictions if any.