Human Gene GFRA3 (ENST00000274721.8) from GENCODE V44
  Description: Homo sapiens GDNF family receptor alpha 3 (GFRA3), mRNA. (from RefSeq NM_001496)
RefSeq Summary (NM_001496): The protein encoded by this gene is a glycosylphosphatidylinositol(GPI)-linked cell surface receptor and a member of the GDNF receptor family. It forms a signaling receptor complex with RET tyrosine kinase receptor and binds the ligand, artemin. [provided by RefSeq, Jul 2008].
Gencode Transcript: ENST00000274721.8
Gencode Gene: ENSG00000146013.11
Transcript (Including UTRs)
   Position: hg38 chr5:138,252,380-138,274,621 Size: 22,242 Total Exon Count: 8 Strand: -
Coding Region
   Position: hg38 chr5:138,252,968-138,274,424 Size: 21,457 Coding Exon Count: 8 

Page IndexSequence and LinksUniProtKB CommentsPrimersCTDRNA-Seq Expression
Microarray ExpressionRNA StructureProtein StructureOther SpeciesGO AnnotationsmRNA Descriptions
PathwaysOther NamesMethods
Data last updated at UCSC: 2023-08-18 00:09:47

-  Sequence and Links to Tools and Databases
Genomic Sequence (chr5:138,252,380-138,274,621)mRNA (may differ from genome)Protein (400 aa)
Gene SorterGenome BrowserOther Species FASTAGene interactionsTable SchemaAlphaFold
BioGPSEnsemblEntrez GeneExonPrimerGencodeGeneCards
OMIMPubMedReactomeUniProtKBWikipediaBioGrid CRISPR DB

-  Comments and Description Text from UniProtKB
DESCRIPTION: RecName: Full=GDNF family receptor alpha-3; Short=GDNF receptor alpha-3; Short=GDNFR-alpha-3; Short=GFR-alpha-3; Flags: Precursor;
FUNCTION: Receptor for the glial cell line-derived neurotrophic factor, ARTN (artemin). Mediates the artemin-induced autophosphorylation and activation of the RET receptor tyrosine kinase.
SUBCELLULAR LOCATION: Cell membrane; Lipid-anchor, GPI-anchor.
TISSUE SPECIFICITY: Widely expressed in adult and fetus which exhibit a similar pattern. Essentially not expressed in the central nervous system, but highly expressed in several sensory and sympathetic ganglia of the peripheral nervous system. Moderate expression in many non-neuronal tissues, particularly those of the digestive and urogenital systems, but high expression in stomach and appendix. Several types of glandular tissues show low expression. Very low or no expression detected in the hematopoietic system.
PTM: N-glycosylated.
SIMILARITY: Belongs to the GDNFR family.

-  Primer design for this transcript

Primer3Plus can design qPCR Primers that straddle exon-exon-junctions, which amplify only cDNA, not genomic DNA.
Click here to load the transcript sequence and exon structure into Primer3Plus

Exonprimer can design one pair of Sanger sequencing primers around every exon, located in non-genic sequence.
Click here to open Exonprimer with this transcript

To design primers for a non-coding sequence, zoom to a region of interest and select from the drop-down menu: View > In External Tools > Primer3

-  Comparative Toxicogenomics Database (CTD)
  The following chemicals interact with this gene           more ... click here to view the complete list

-  RNA-Seq Expression Data from GTEx (53 Tissues, 570 Donors)
  Highest median expression: 26.57 RPKM in Nerve - Tibial
Total median expression: 87.23 RPKM

View in GTEx track of Genome Browser    View at GTEx portal     View GTEx Body Map

+  Microarray Expression Data
  Press "+" in the title bar above to open this section.

-  mRNA Secondary Structure of 3' and 5' UTRs
RegionFold EnergyBasesEnergy/Base
Display As
5' UTR -104.90197-0.532 Picture PostScript Text
3' UTR -202.20588-0.344 Picture PostScript Text

The RNAfold program from the Vienna RNA Package is used to perform the secondary structure predictions and folding calculations. The estimated folding energy is in kcal/mol. The more negative the energy, the more secondary structure the RNA is likely to have.

-  Protein Domain and Structure Information
  InterPro Domains: Graphical view of domain structure
IPR016017 - GDNF/GAS1
IPR003438 - GDNF_rcpt
IPR003505 - GDNF_rcpt_A3

Pfam Domains:
PF02351 - GDNF/GAS1 domain

Protein Data Bank (PDB) 3-D Structure
MuPIT help
2GH0 - X-ray MuPIT

ModBase Predicted Comparative 3D Structure on O60609
The pictures above may be empty if there is no ModBase structure for the protein. The ModBase structure frequently covers just a fragment of the protein. You may be asked to log onto ModBase the first time you click on the pictures. It is simplest after logging in to just click on the picture again to get to the specific info on that model.

-  Orthologous Genes in Other Species
  Orthologies between human, mouse, and rat are computed by taking the best BLASTP hit, and filtering out non-syntenic hits. For more distant species reciprocal-best BLASTP hits are used. Note that the absence of an ortholog in the table below may reflect incomplete annotations in the other species rather than a true absence of the orthologous gene.
MouseRatZebrafishD. melanogasterC. elegansS. cerevisiae
Genome BrowserGenome BrowserNo orthologNo orthologNo orthologNo ortholog
Gene Details     
Gene Sorter     
Protein SequenceProtein Sequence    

-  Gene Ontology (GO) Annotations with Structured Vocabulary
  Molecular Function:
GO:0005088 Ras guanyl-nucleotide exchange factor activity
GO:0005102 receptor binding
GO:0005515 protein binding
GO:0008046 axon guidance receptor activity
GO:0038023 signaling receptor activity

Biological Process:
GO:0000165 MAPK cascade
GO:0001764 neuron migration
GO:0007165 signal transduction
GO:0007399 nervous system development
GO:0007411 axon guidance
GO:0007422 peripheral nervous system development
GO:0048485 sympathetic nervous system development
GO:0048666 neuron development

Cellular Component:
GO:0005829 cytosol
GO:0005886 plasma membrane
GO:0009897 external side of plasma membrane
GO:0016020 membrane
GO:0019898 extrinsic component of membrane
GO:0031225 anchored component of membrane

-  Descriptions from all associated GenBank mRNAs
  AY358997 - Homo sapiens clone DNA48613 GFRA3 (UNQ339) mRNA, complete cds.
AF086292 - Homo sapiens full length insert cDNA clone ZD48G06.
BC037951 - Homo sapiens GDNF family receptor alpha 3, mRNA (cDNA clone MGC:45283 IMAGE:5227725), complete cds.
AY359037 - Homo sapiens clone DNA48614 GFRA3 (UNQ339) mRNA, complete cds.
AK297693 - Homo sapiens cDNA FLJ52334 complete cds, highly similar to GDNF family receptor alpha-3 precursor.
AF051767 - Homo sapiens GDNF family receptor alpha 3 (GFRA3) mRNA, complete cds.
FJ200487 - Homo sapiens GDNF family receptor alpha-3 variant (GDNFR3) mRNA, complete cds.
DQ890850 - Synthetic construct clone IMAGE:100003480; FLH166451.01X; RZPDo839G1286D GDNF family receptor alpha 3 (GFRA3) gene, encodes complete protein.
AK314022 - Homo sapiens cDNA, FLJ94680, Homo sapiens GDNF family receptor alpha 3 (GFRA3), mRNA.
DQ894004 - Synthetic construct Homo sapiens clone IMAGE:100008464; FLH166447.01L; RZPDo839G1285D GDNF family receptor alpha 3 (GFRA3) gene, encodes complete protein.

-  Biochemical and Signaling Pathways
  Reactome (by CSHL, EBI, and GO)

Protein O60609 (Reactome details) participates in the following event(s):

R-HSA-8853745 RET binds GFRA1,GFRA3
R-HSA-8853800 ARTN binds RET:GFRA1,GFRA3
R-HSA-8853762 RET dimerizes
R-HSA-8854908 PKA phosphorylates RET:GDNF:GFRA dimer
R-HSA-8853737 2x p-5Y-RET:GDNF:GFRA complexes bind SHC1
R-HSA-8853753 2x p-5Y-RET:GDNF:GFRA complexes bind GRB7,10
R-HSA-8853755 2x p-5Y-RET:GDNF:GFRA complexes bind PLCgamma1
R-HSA-8854899 2x p-5Y-RET:GDNF:GFRA complexes bind GRB2-1:SOS1
R-HSA-8855564 2x p-5Y-RET:GDNF:GFRA complexes bind FRS2
R-HSA-8855617 2x p-5Y-RET:GDNF:GFRA complexes binds DOK1,DOK2,DOK4,DOK5,DOK6
R-HSA-8855747 2x p-5Y-RET:GDNF:GFRA complexes bind SRC1, RAP1GAP
R-HSA-8855915 2x p-5Y-RET:GDNF:GFRA complexes bind RET interactors
R-HSA-8853792 RET tyrosine phosphorylation
R-HSA-8853734 2x p-5Y-RET:GDNF:GFRA complexes:p-Y349,Y350,Y427-SHC1 binds GRB2-1:SOS1
R-HSA-5672965 RAS GEFs promote RAS nucleotide exchange
R-HSA-8855508 p-5Y-GAB in RET-GRB2-GAB complexes binds PTPN11
R-HSA-8853659 RET signaling
R-HSA-422475 Axon guidance
R-HSA-1266738 Developmental Biology
R-HSA-5673001 RAF/MAP kinase cascade
R-HSA-5684996 MAPK1/MAPK3 signaling
R-HSA-5683057 MAPK family signaling cascades
R-HSA-162582 Signal Transduction

-  Other Names for This Gene
  Alternate Gene Symbols: B2RA36, B4DMY9, ENST00000274721.1, ENST00000274721.2, ENST00000274721.3, ENST00000274721.4, ENST00000274721.5, ENST00000274721.6, ENST00000274721.7, GFRA3_HUMAN, NM_001496, O60609, Q6UW20, Q8IUZ2, uc003lcn.1, uc003lcn.2, uc003lcn.3, uc003lcn.4, uc003lcn.5, UNQ339/PRO538/PRO3664
UCSC ID: ENST00000274721.8
RefSeq Accession: NM_001496
Protein: O60609 (aka GFRA3_HUMAN or GFR3_HUMAN)
CCDS: CCDS4201.1

-  Methods, Credits, and Use Restrictions
  Click here for details on how this gene model was made and data restrictions if any.