Human Gene DBNL (ENST00000494774.5) from GENCODE V44
  Description: Homo sapiens drebrin like (DBNL), transcript variant 1, mRNA. (from RefSeq NM_014063)
Gencode Transcript: ENST00000494774.5
Gencode Gene: ENSG00000136279.21
Transcript (Including UTRs)
   Position: hg38 chr7:44,044,717-44,061,716 Size: 17,000 Total Exon Count: 13 Strand: +
Coding Region
   Position: hg38 chr7:44,044,738-44,060,916 Size: 16,179 Coding Exon Count: 13 

Page IndexSequence and LinksUniProtKB CommentsPrimersCTDRNA-Seq Expression
Microarray ExpressionRNA StructureProtein StructureOther SpeciesGO AnnotationsmRNA Descriptions
PathwaysOther NamesMethods
Data last updated at UCSC: 2023-08-18 00:09:47

-  Sequence and Links to Tools and Databases
 
Genomic Sequence (chr7:44,044,717-44,061,716)mRNA (may differ from genome)Protein (431 aa)
Gene SorterGenome BrowserOther Species FASTAGene interactionsTable SchemaAlphaFold
BioGPSEnsemblEntrez GeneExonPrimerGencodeGeneCards
HGNCHPRDLynxMalacardsMGImyGene2
neXtProtOMIMPubMedReactomeUniProtKBWikipedia
BioGrid CRISPR DB

-  Comments and Description Text from UniProtKB
  ID: DBNL_HUMAN
DESCRIPTION: RecName: Full=Drebrin-like protein; AltName: Full=Cervical SH3P7; AltName: Full=Cervical mucin-associated protein; AltName: Full=Drebrin-F; AltName: Full=HPK1-interacting protein of 55 kDa; Short=HIP-55; AltName: Full=SH3 domain-containing protein 7;
FUNCTION: Actin-binding adapter protein. May act as a common effector of antigen receptor-signaling pathways in leukocytes. Its association with dynamin suggests that it may also connect the actin cytoskeleton to endocytic function. Acts as a key component of the immunological synapse that regulates T-cell activation by bridging TCRs and the actin cytoskeleton to gene activation and endocytic processes. Binds to F-actin but is not involved in actin polymerization, capping or bundling. Does not bind G-actin.
SUBUNIT: Interacts with SHANK2 and SHANK3 (By similarity). Interacts with FGD1, dynamin, MAP4K1 and PRAM1. Interacts with ANKRD54 (By similarity).
INTERACTION: Q9H788:SH2D4A; NbExp=3; IntAct=EBI-751783, EBI-747035;
SUBCELLULAR LOCATION: Cytoplasm, cytoskeleton. Cell projection, lamellipodium. Note=Cortical cytoskeleton. Associates with lamellipodial actin.
DOMAIN: The SH3 domain mediates interaction with SHANK2, SHANK3 and PRAM1.
PTM: Degraded by caspases during apoptosis.
SIMILARITY: Belongs to the ABP1 family.
SIMILARITY: Contains 1 ADF-H domain.
SIMILARITY: Contains 1 SH3 domain.

-  Primer design for this transcript
 

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Click here to load the transcript sequence and exon structure into Primer3Plus

Exonprimer can design one pair of Sanger sequencing primers around every exon, located in non-genic sequence.
Click here to open Exonprimer with this transcript

To design primers for a non-coding sequence, zoom to a region of interest and select from the drop-down menu: View > In External Tools > Primer3


-  Comparative Toxicogenomics Database (CTD)
  The following chemicals interact with this gene           more ... click here to view the complete list

-  RNA-Seq Expression Data from GTEx (53 Tissues, 570 Donors)
  Highest median expression: 36.13 RPKM in Whole Blood
Total median expression: 667.68 RPKM



View in GTEx track of Genome Browser    View at GTEx portal     View GTEx Body Map

+  Microarray Expression Data
  Press "+" in the title bar above to open this section.

-  mRNA Secondary Structure of 3' and 5' UTRs
 
RegionFold EnergyBasesEnergy/Base
Display As
5' UTR -3.6021-0.171 Picture PostScript Text
3' UTR -294.30800-0.368 Picture PostScript Text

The RNAfold program from the Vienna RNA Package is used to perform the secondary structure predictions and folding calculations. The estimated folding energy is in kcal/mol. The more negative the energy, the more secondary structure the RNA is likely to have.

-  Protein Domain and Structure Information
  InterPro Domains: Graphical view of domain structure
IPR002108 - Actin-bd_cofilin/tropomyosin
IPR001452 - SH3_domain

Pfam Domains:
PF00241 - Cofilin/tropomyosin-type actin-binding protein
PF00018 - SH3 domain

Protein Data Bank (PDB) 3-D Structure
MuPIT help
1X67 - NMR MuPIT


ModBase Predicted Comparative 3D Structure on Q9UJU6
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The pictures above may be empty if there is no ModBase structure for the protein. The ModBase structure frequently covers just a fragment of the protein. You may be asked to log onto ModBase the first time you click on the pictures. It is simplest after logging in to just click on the picture again to get to the specific info on that model.

-  Orthologous Genes in Other Species
  Orthologies between human, mouse, and rat are computed by taking the best BLASTP hit, and filtering out non-syntenic hits. For more distant species reciprocal-best BLASTP hits are used. Note that the absence of an ortholog in the table below may reflect incomplete annotations in the other species rather than a true absence of the orthologous gene.
MouseRatZebrafishD. melanogasterC. elegansS. cerevisiae
Genome BrowserGenome BrowserNo orthologNo orthologNo orthologNo ortholog
Gene Details     
Gene Sorter     
MGIRGD    
Protein SequenceProtein Sequence    
AlignmentAlignment    

-  Gene Ontology (GO) Annotations with Structured Vocabulary
  Molecular Function:
GO:0003779 actin binding
GO:0005515 protein binding
GO:0008022 protein C-terminus binding
GO:0008047 enzyme activator activity
GO:0019904 protein domain specific binding
GO:0045296 cadherin binding
GO:0051015 actin filament binding

Biological Process:
GO:0002250 adaptive immune response
GO:0002376 immune system process
GO:0006897 endocytosis
GO:0006898 receptor-mediated endocytosis
GO:0007257 activation of JUN kinase activity
GO:0007416 synapse assembly
GO:0016601 Rac protein signal transduction
GO:0043312 neutrophil degranulation
GO:0048812 neuron projection morphogenesis
GO:0071800 podosome assembly
GO:0097178 ruffle assembly

Cellular Component:
GO:0000139 Golgi membrane
GO:0001726 ruffle
GO:0002102 podosome
GO:0005576 extracellular region
GO:0005622 intracellular
GO:0005737 cytoplasm
GO:0005768 endosome
GO:0005769 early endosome
GO:0005794 Golgi apparatus
GO:0005829 cytosol
GO:0005856 cytoskeleton
GO:0005886 plasma membrane
GO:0005938 cell cortex
GO:0014069 postsynaptic density
GO:0016020 membrane
GO:0030027 lamellipodium
GO:0030054 cell junction
GO:0030425 dendrite
GO:0030665 clathrin-coated vesicle membrane
GO:0031410 cytoplasmic vesicle
GO:0034774 secretory granule lumen
GO:0042995 cell projection
GO:0045202 synapse
GO:0070062 extracellular exosome
GO:1904724 tertiary granule lumen
GO:1904813 ficolin-1-rich granule lumen


-  Descriptions from all associated GenBank mRNAs
  AK293279 - Homo sapiens cDNA FLJ52531 complete cds, highly similar to Drebrin-like protein.
AK293698 - Homo sapiens cDNA FLJ59481 complete cds, highly similar to Drebrin-like protein.
AF077353 - Homo sapiens drebrin F mRNA, complete cds.
AK298102 - Homo sapiens cDNA FLJ59941 complete cds, highly similar to Drebrin-like protein.
AK296778 - Homo sapiens cDNA FLJ54071 complete cds, highly similar to Drebrin-like protein.
AK302037 - Homo sapiens cDNA FLJ53677 complete cds, highly similar to Drebrin-like protein.
AK293151 - Homo sapiens cDNA FLJ59450 complete cds, highly similar to Drebrin-like protein.
AF218020 - Homo sapiens PP5423 mRNA, complete cds.
AK293340 - Homo sapiens cDNA FLJ54066 complete cds, highly similar to Drebrin-like protein.
AK027367 - Homo sapiens cDNA FLJ14461 fis, clone MAMMA1000173, highly similar to Homo sapiens src homology 3 domain-containing protein HIP-55 mRNA.
BC011677 - Homo sapiens drebrin-like, mRNA (cDNA clone MGC:15142 IMAGE:4299867), complete cds.
AK300630 - Homo sapiens cDNA FLJ59432 complete cds, highly similar to Drebrin-like protein.
AF151364 - Homo sapiens mucin-associated protein (CMAP) mRNA, complete cds.
JD217684 - Sequence 198708 from Patent EP1572962.
BC031687 - Homo sapiens drebrin-like, mRNA (cDNA clone MGC:35124 IMAGE:5168099), complete cds.
AB527593 - Synthetic construct DNA, clone: pF1KB6610, Homo sapiens DBNL gene for drebrin-like protein, without stop codon, in Flexi system.
CU680006 - Synthetic construct Homo sapiens gateway clone IMAGE:100019367 5' read DBNL mRNA.
CU689228 - Synthetic construct Homo sapiens gateway clone IMAGE:100018271 5' read DBNL mRNA.
HQ447892 - Synthetic construct Homo sapiens clone IMAGE:100071247; CCSB009440_02 drebrin-like (DBNL) gene, encodes complete protein.
JF432143 - Synthetic construct Homo sapiens clone IMAGE:100073286 drebrin-like (DBNL) gene, encodes complete protein.
KJ893665 - Synthetic construct Homo sapiens clone ccsbBroadEn_03059 DBNL gene, encodes complete protein.
CR457167 - Homo sapiens full open reading frame cDNA clone RZPDo834C1012D for gene HIP-55, src homology 3 domain-containing protein HIP-55; complete cds, incl. stopcodon.
KJ893664 - Synthetic construct Homo sapiens clone ccsbBroadEn_03058 DBNL gene, encodes complete protein.
AF250287 - Homo sapiens cervical SH3P7 mRNA, complete cds.
AF197060 - Homo sapiens src homology 3 domain-containing protein HIP-55 mRNA, complete cds.
BC023589 - Homo sapiens drebrin-like, mRNA (cDNA clone IMAGE:4843899), with apparent retained intron.
AB209486 - Homo sapiens mRNA for Drebrin-like protein variant protein.
JD021354 - Sequence 2378 from Patent EP1572962.
JD029433 - Sequence 10457 from Patent EP1572962.
AK126142 - Homo sapiens cDNA FLJ44154 fis, clone THYMU2032014, highly similar to Homo sapiens src homology 3 domain-containing protein HIP-55 (HIP-55).
JD328871 - Sequence 309895 from Patent EP1572962.
JD076872 - Sequence 57896 from Patent EP1572962.
JD416613 - Sequence 397637 from Patent EP1572962.
JD054260 - Sequence 35284 from Patent EP1572962.
JD535255 - Sequence 516279 from Patent EP1572962.
JD229191 - Sequence 210215 from Patent EP1572962.
JD164130 - Sequence 145154 from Patent EP1572962.
JD137739 - Sequence 118763 from Patent EP1572962.
JD162641 - Sequence 143665 from Patent EP1572962.
JD188780 - Sequence 169804 from Patent EP1572962.
JD422221 - Sequence 403245 from Patent EP1572962.
JD068761 - Sequence 49785 from Patent EP1572962.
JD483529 - Sequence 464553 from Patent EP1572962.
JD526875 - Sequence 507899 from Patent EP1572962.
JD264425 - Sequence 245449 from Patent EP1572962.
JD068154 - Sequence 49178 from Patent EP1572962.
JD423814 - Sequence 404838 from Patent EP1572962.
JD272112 - Sequence 253136 from Patent EP1572962.
JD425847 - Sequence 406871 from Patent EP1572962.
JD482276 - Sequence 463300 from Patent EP1572962.
JD353773 - Sequence 334797 from Patent EP1572962.
JD463688 - Sequence 444712 from Patent EP1572962.

-  Biochemical and Signaling Pathways
  Reactome (by CSHL, EBI, and GO)

Protein Q9UJU6 (Reactome details) participates in the following event(s):

R-HSA-202966 Caspase mediated cleavage of HIP-55
R-HSA-6797554 ABP1 binds SHANK proteins
R-HSA-6800434 Exocytosis of ficolin-rich granule lumen proteins
R-HSA-6798745 Exocytosis of tertiary granule lumen proteins
R-HSA-6798748 Exocytosis of secretory granule lumen proteins
R-HSA-264870 Caspase-mediated cleavage of cytoskeletal proteins
R-HSA-6794361 Neurexins and neuroligins
R-HSA-111465 Apoptotic cleavage of cellular proteins
R-HSA-6794362 Protein-protein interactions at synapses
R-HSA-6798695 Neutrophil degranulation
R-HSA-75153 Apoptotic execution phase
R-HSA-112316 Neuronal System
R-HSA-168249 Innate Immune System
R-HSA-109581 Apoptosis
R-HSA-168256 Immune System
R-HSA-5357801 Programmed Cell Death

-  Other Names for This Gene
  Alternate Gene Symbols: A4D2I9, CMAP, DBNL_HUMAN, ENST00000494774.1, ENST00000494774.2, ENST00000494774.3, ENST00000494774.4, NM_014063, P84070, PP5423, Q6IAI8, Q96F30, Q96K74, Q9HBN8, Q9NR72, Q9UJU6, SH3P7, uc003tjo.1, uc003tjo.2, uc003tjo.3, uc003tjo.4, uc003tjo.5
UCSC ID: ENST00000494774.5
RefSeq Accession: NM_014063
Protein: Q9UJU6 (aka DBNL_HUMAN)
CCDS: CCDS34622.1, CCDS34623.1, CCDS47579.1

-  Methods, Credits, and Use Restrictions
  Click here for details on how this gene model was made and data restrictions if any.