Human Gene TES (ENST00000358204.9) from GENCODE V44
  Description: Homo sapiens testin LIM domain protein (TES), transcript variant 1, mRNA. (from RefSeq NM_015641)
RefSeq Summary (NM_015641): Cancer-associated chromosomal changes often involve regions containing fragile sites. This gene maps to a commom fragile site on chromosome 7q31.2 designated FRA7G. This gene is similar to mouse Testin, a testosterone-responsive gene encoding a Sertoli cell secretory protein containing three LIM domains. LIM domains are double zinc-finger motifs that mediate protein-protein interactions between transcription factors, cytoskeletal proteins and signaling proteins. This protein is a negative regulator of cell growth and may act as a tumor suppressor. This scaffold protein may also play a role in cell adhesion, cell spreading and in the reorganization of the actin cytoskeleton. Multiple protein isoforms are encoded by transcript variants of this gene.[provided by RefSeq, Mar 2011].
Gencode Transcript: ENST00000358204.9
Gencode Gene: ENSG00000135269.18
Transcript (Including UTRs)
   Position: hg38 chr7:116,210,539-116,258,783 Size: 48,245 Total Exon Count: 7 Strand: +
Coding Region
   Position: hg38 chr7:116,210,708-116,257,482 Size: 46,775 Coding Exon Count: 7 

Page IndexSequence and LinksUniProtKB CommentsPrimersMalaCardsCTD
RNA-Seq ExpressionMicroarray ExpressionRNA StructureProtein StructureOther SpeciesGO Annotations
mRNA DescriptionsOther NamesMethods
Data last updated at UCSC: 2023-08-18 00:09:47

-  Sequence and Links to Tools and Databases
 
Genomic Sequence (chr7:116,210,539-116,258,783)mRNA (may differ from genome)Protein (421 aa)
Gene SorterGenome BrowserOther Species FASTAGene interactionsTable SchemaAlphaFold
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OMIMPubMedUniProtKBWikipediaBioGrid CRISPR DB

-  Comments and Description Text from UniProtKB
  ID: TES_HUMAN
DESCRIPTION: RecName: Full=Testin; AltName: Full=TESS;
FUNCTION: Scaffold protein that may play a role in cell adhesion, cell spreading and in the reorganization of the actin cytoskeleton. Plays a role in the regulation of cell proliferation. May act as a tumor suppressor. Inhibits tumor cell growth.
SUBUNIT: Interacts via LIM domain 1 with ZYX. Interacts (via LIM domain 3) with ENAH and VASP. Interacts with talin, actin, alpha- actinin, GRIP1 and PXN. Interacts (via LIM domain 2) with ACTL7A (via N-terminus). Heterodimer with ACTL7A; the heterodimer interacts with ENAH to form a heterotrimer.
SUBCELLULAR LOCATION: Cytoplasm. Cell junction, focal adhesion. Note=Detected along actin stress fibers.
TISSUE SPECIFICITY: Ubiquitous.
DOMAIN: The N-terminal and the C-terminal halves of the protein can associate with each other, thereby hindering interactions with ZYX.
SIMILARITY: Belongs to the prickle / espinas / testin family.
SIMILARITY: Contains 3 LIM zinc-binding domains.
SIMILARITY: Contains 1 PET domain.

-  Primer design for this transcript
 

Primer3Plus can design qPCR Primers that straddle exon-exon-junctions, which amplify only cDNA, not genomic DNA.
Click here to load the transcript sequence and exon structure into Primer3Plus

Exonprimer can design one pair of Sanger sequencing primers around every exon, located in non-genic sequence.
Click here to open Exonprimer with this transcript

To design primers for a non-coding sequence, zoom to a region of interest and select from the drop-down menu: View > In External Tools > Primer3


-  MalaCards Disease Associations
  MalaCards Gene Search: TES
Diseases sorted by gene-association score: louping ill (2), diphyllobothriasis (1)

-  Comparative Toxicogenomics Database (CTD)
  The following chemicals interact with this gene
  • D013749 Tetrachlorodibenzodioxin
  • D000082 Acetaminophen
  • D003993 Dibutyl Phthalate
  • C022838 nickel chloride
  • C517041 (4-amino-1,4-dihydro-3-(2-pyridyl)-5-thioxo-1,2,4-triazole)copper(II)
  • C548651 2-(1'H-indolo-3'-carbonyl)thiazole-4-carboxylic acid methyl ester
  • D015073 2-Acetylaminofluorene
  • C472791 3-(4'-hydroxy-3'-adamantylbiphenyl-4-yl)acrylic acid
  • C009505 4,4'-diaminodiphenylmethane
  • D015123 7,8-Dihydro-7,8-dihydroxybenzo(a)pyrene 9,10-oxide
          more ... click here to view the complete list

-  RNA-Seq Expression Data from GTEx (53 Tissues, 570 Donors)
  Highest median expression: 68.75 RPKM in Colon - Sigmoid
Total median expression: 730.19 RPKM



View in GTEx track of Genome Browser    View at GTEx portal     View GTEx Body Map

+  Microarray Expression Data
  Press "+" in the title bar above to open this section.

-  mRNA Secondary Structure of 3' and 5' UTRs
 
RegionFold EnergyBasesEnergy/Base
Display As
5' UTR -78.70169-0.466 Picture PostScript Text
3' UTR -307.801301-0.237 Picture PostScript Text

The RNAfold program from the Vienna RNA Package is used to perform the secondary structure predictions and folding calculations. The estimated folding energy is in kcal/mol. The more negative the energy, the more secondary structure the RNA is likely to have.

-  Protein Domain and Structure Information
  InterPro Domains: Graphical view of domain structure
IPR010442 - PET_domain
IPR001781 - Znf_LIM

Pfam Domains:
PF00412 - LIM domain
PF06297 - PET Domain

Protein Data Bank (PDB) 3-D Structure
MuPIT help
2IYB - X-ray MuPIT 2XQN - X-ray MuPIT


ModBase Predicted Comparative 3D Structure on Q9UGI8
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The pictures above may be empty if there is no ModBase structure for the protein. The ModBase structure frequently covers just a fragment of the protein. You may be asked to log onto ModBase the first time you click on the pictures. It is simplest after logging in to just click on the picture again to get to the specific info on that model.

-  Orthologous Genes in Other Species
  Orthologies between human, mouse, and rat are computed by taking the best BLASTP hit, and filtering out non-syntenic hits. For more distant species reciprocal-best BLASTP hits are used. Note that the absence of an ortholog in the table below may reflect incomplete annotations in the other species rather than a true absence of the orthologous gene.
MouseRatZebrafishD. melanogasterC. elegansS. cerevisiae
Genome BrowserGenome BrowserGenome BrowserNo orthologGenome BrowserNo ortholog
Gene Details     
Gene Sorter     
MGIRGDEnsembl WormBase 
Protein SequenceProtein SequenceProtein Sequence Protein Sequence 
AlignmentAlignmentAlignment Alignment 

-  Gene Ontology (GO) Annotations with Structured Vocabulary
  Molecular Function:
GO:0003723 RNA binding
GO:0005515 protein binding
GO:0008270 zinc ion binding
GO:0045296 cadherin binding
GO:0046872 metal ion binding

Biological Process:
GO:0008285 negative regulation of cell proliferation
GO:0042127 regulation of cell proliferation

Cellular Component:
GO:0005634 nucleus
GO:0005737 cytoplasm
GO:0005829 cytosol
GO:0005886 plasma membrane
GO:0005925 focal adhesion
GO:0030054 cell junction
GO:0032991 macromolecular complex


-  Descriptions from all associated GenBank mRNAs
  AK299104 - Homo sapiens cDNA FLJ59160 complete cds, highly similar to Testin.
BC001451 - Homo sapiens testis derived transcript (3 LIM domains), mRNA (cDNA clone MGC:1146 IMAGE:3138867), complete cds.
LP895574 - Sequence 438 from Patent EP3253886.
AK021575 - Homo sapiens cDNA FLJ11513 fis, clone HEMBA1002215, highly similar to TESTIN 2 (TES2) [CONTAINS: TESTIN 1 (TES1)].
AK300546 - Homo sapiens cDNA FLJ50681 complete cds, highly similar to Testin.
AK309324 - Homo sapiens cDNA, FLJ99365.
JD500506 - Sequence 481530 from Patent EP1572962.
AK222840 - Homo sapiens mRNA for testin isoform 1 variant, clone: HEP07833.
AF245356 - Homo sapiens TESTIN 2 mRNA, complete cds.
AJ250865 - Homo sapiens mRNA for TESS 2 protein (TESS 2 gene).
AK315644 - Homo sapiens cDNA, FLJ96732, highly similar to Homo sapiens testis derived transcript (3 LIM domains) (TES), transcript variant 1, mRNA.
JD394750 - Sequence 375774 from Patent EP1572962.
KJ893528 - Synthetic construct Homo sapiens clone ccsbBroadEn_02922 TES gene, encodes complete protein.
AB529039 - Synthetic construct DNA, clone: pF1KB3479, Homo sapiens TES gene for testis derived transcript, without stop codon, in Flexi system.
EU176352 - Synthetic construct Homo sapiens clone IMAGE:100006573; FLH263891.01X; RZPDo839B09247D testis derived transcript (3 LIM domains) (TES) gene, encodes complete protein.
EU176694 - Synthetic construct Homo sapiens clone IMAGE:100011611; FLH263884.01L; RZPDo839B05247D testis derived transcript (3 LIM domains) (TES) gene, encodes complete protein.
AK310995 - Homo sapiens cDNA, FLJ18037.
AK291802 - Homo sapiens cDNA FLJ76896 complete cds, highly similar to Homo sapiens testis derived transcript (3 LIM domains) (TES), transcript variant 2, mRNA.
AF245357 - Homo sapiens TESTIN 3 mRNA, complete cds.
GQ423971 - Homo sapiens testin variant (TES) mRNA, partial cds, alternatively spliced.
AL050162 - Homo sapiens mRNA; cDNA DKFZp586B2022 (from clone DKFZp586B2022); partial cds.
DQ576816 - Homo sapiens piRNA piR-44928, complete sequence.
JD304167 - Sequence 285191 from Patent EP1572962.
JD296579 - Sequence 277603 from Patent EP1572962.
JD223985 - Sequence 205009 from Patent EP1572962.
JD048893 - Sequence 29917 from Patent EP1572962.
JD491136 - Sequence 472160 from Patent EP1572962.
JD552351 - Sequence 533375 from Patent EP1572962.
AF147329 - Homo sapiens full length insert cDNA clone YB23C09.
JD441306 - Sequence 422330 from Patent EP1572962.
JD441305 - Sequence 422329 from Patent EP1572962.
JD328765 - Sequence 309789 from Patent EP1572962.
JD328766 - Sequence 309790 from Patent EP1572962.
JD312170 - Sequence 293194 from Patent EP1572962.
JD346651 - Sequence 327675 from Patent EP1572962.
JD374472 - Sequence 355496 from Patent EP1572962.
JD378186 - Sequence 359210 from Patent EP1572962.
JD051961 - Sequence 32985 from Patent EP1572962.
JD268543 - Sequence 249567 from Patent EP1572962.
JD161222 - Sequence 142246 from Patent EP1572962.

-  Other Names for This Gene
  Alternate Gene Symbols: A4D0U6, ENST00000358204.1, ENST00000358204.2, ENST00000358204.3, ENST00000358204.4, ENST00000358204.5, ENST00000358204.6, ENST00000358204.7, ENST00000358204.8, NM_015641, Q9GZQ1, Q9HAJ9, Q9UGI8, TES_HUMAN, uc003vho.1, uc003vho.2, uc003vho.3, uc003vho.4
UCSC ID: ENST00000358204.9
RefSeq Accession: NM_015641
Protein: Q9UGI8 (aka TES_HUMAN)
CCDS: CCDS5764.1, CCDS5763.1

-  Methods, Credits, and Use Restrictions
  Click here for details on how this gene model was made and data restrictions if any.