Human Gene OPRK1 (ENST00000265572.8) from GENCODE V44
  Description: Homo sapiens opioid receptor kappa 1 (OPRK1), transcript variant 1, mRNA. (from RefSeq NM_000912)
RefSeq Summary (NM_000912): This gene encodes an opioid receptor, which is a member of the 7 transmembrane-spanning G protein-coupled receptor family. It functions as a receptor for endogenous ligands, as well as a receptor for various synthetic opioids. Ligand binding results in inhibition of adenylate cyclase activity and neurotransmitter release. This opioid receptor plays a role in the perception of pain and mediating the hypolocomotor, analgesic and aversive actions of synthetic opioids. Variations in this gene have also been associated with alcohol dependence and opiate addiction. Alternatively spliced transcript variants encoding different isoforms have been found for this gene. A recent study provided evidence for translational readthrough in this gene, and expression of an additional C-terminally extended isoform via the use of an alternative in-frame translation termination codon. [provided by RefSeq, Dec 2017].
Gencode Transcript: ENST00000265572.8
Gencode Gene: ENSG00000082556.14
Transcript (Including UTRs)
   Position: hg38 chr8:53,225,724-53,251,637 Size: 25,914 Total Exon Count: 4 Strand: -
Coding Region
   Position: hg38 chr8:53,229,297-53,251,037 Size: 21,741 Coding Exon Count: 3 

Page IndexSequence and LinksUniProtKB CommentsPrimersMalaCardsCTD
RNA-Seq ExpressionMicroarray ExpressionRNA StructureProtein StructureOther SpeciesGO Annotations
mRNA DescriptionsPathwaysOther NamesMethods
Data last updated at UCSC: 2023-08-18 00:09:47

-  Sequence and Links to Tools and Databases
 
Genomic Sequence (chr8:53,225,724-53,251,637)mRNA (may differ from genome)Protein (380 aa)
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HGNCHPRDLynxMalacardsMGIneXtProt
OMIMPubMedReactomeUniProtKBWikipediaBioGrid CRISPR DB

-  Comments and Description Text from UniProtKB
  ID: OPRK_HUMAN
DESCRIPTION: RecName: Full=Kappa-type opioid receptor; Short=K-OR-1; Short=KOR-1;
FUNCTION: Inhibits neurotransmitter release by reducing calcium ion currents and increasing potassium ion conductance. Receptor for dynorphins. May play a role in arousal and regulation of autonomic and neuroendocrine functions.
SUBUNIT: Interacts with SLC9A3R1. Interacts with GABARAPL1.
INTERACTION: Q9H0R8:GABARAPL1; NbExp=5; IntAct=EBI-925028, EBI-3464833;
SUBCELLULAR LOCATION: Cell membrane; Multi-pass membrane protein.
SIMILARITY: Belongs to the G-protein coupled receptor 1 family.
WEB RESOURCE: Name=Wikipedia; Note=Kappa opioid receptor entry; URL="http://en.wikipedia.org/wiki/Kappa_opioid_receptor";

-  Primer design for this transcript
 

Primer3Plus can design qPCR Primers that straddle exon-exon-junctions, which amplify only cDNA, not genomic DNA.
Click here to load the transcript sequence and exon structure into Primer3Plus

Exonprimer can design one pair of Sanger sequencing primers around every exon, located in non-genic sequence.
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To design primers for a non-coding sequence, zoom to a region of interest and select from the drop-down menu: View > In External Tools > Primer3


-  MalaCards Disease Associations
  MalaCards Gene Search: OPRK1
Diseases sorted by gene-association score: alcohol dependence (13), morphine dependence (13), neonatal abstinence syndrome (11), drug dependence (11), cocaine dependence (11), opiate dependence (10), fuchs' heterochromic uveitis (7), substance dependence (7), uremic pruritus (7), phobic disorder (7), agoraphobia (5), pain agnosia (5), agnosia (5)

-  Comparative Toxicogenomics Database (CTD)
  The following chemicals interact with this gene           more ... click here to view the complete list

-  RNA-Seq Expression Data from GTEx (53 Tissues, 570 Donors)
  Highest median expression: 2.27 RPKM in Brain - Nucleus accumbens (basal ganglia)
Total median expression: 10.90 RPKM



View in GTEx track of Genome Browser    View at GTEx portal     View GTEx Body Map

+  Microarray Expression Data
  Press "+" in the title bar above to open this section.

-  mRNA Secondary Structure of 3' and 5' UTRs
 
RegionFold EnergyBasesEnergy/Base
Display As
5' UTR -113.20238-0.476 Picture PostScript Text
3' UTR -1022.103573-0.286 Picture PostScript Text

The RNAfold program from the Vienna RNA Package is used to perform the secondary structure predictions and folding calculations. The estimated folding energy is in kcal/mol. The more negative the energy, the more secondary structure the RNA is likely to have.

-  Protein Domain and Structure Information
  InterPro Domains: Graphical view of domain structure
IPR000276 - 7TM_GPCR_Rhodpsn
IPR017452 - GPCR_Rhodpsn_supfam
IPR000452 - Kappa_opi_rcpt
IPR001418 - Opioid_rcpt

Pfam Domains:
PF00001 - 7 transmembrane receptor (rhodopsin family)

Protein Data Bank (PDB) 3-D Structure
MuPIT help
2A0D - Model 2IQN - Model 4DJH - X-ray MuPIT


ModBase Predicted Comparative 3D Structure on P41145
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-  Orthologous Genes in Other Species
  Orthologies between human, mouse, and rat are computed by taking the best BLASTP hit, and filtering out non-syntenic hits. For more distant species reciprocal-best BLASTP hits are used. Note that the absence of an ortholog in the table below may reflect incomplete annotations in the other species rather than a true absence of the orthologous gene.
MouseRatZebrafishD. melanogasterC. elegansS. cerevisiae
Genome BrowserGenome BrowserNo orthologNo orthologNo orthologNo ortholog
Gene Details     
Gene Sorter     
MGIRGD    
Protein SequenceProtein Sequence    
AlignmentAlignment    

-  Gene Ontology (GO) Annotations with Structured Vocabulary
  Molecular Function:
GO:0004871 signal transducer activity
GO:0004930 G-protein coupled receptor activity
GO:0004985 opioid receptor activity
GO:0005515 protein binding
GO:0033612 receptor serine/threonine kinase binding
GO:0038048 dynorphin receptor activity
GO:0042923 neuropeptide binding

Biological Process:
GO:0006955 immune response
GO:0007165 signal transduction
GO:0007186 G-protein coupled receptor signaling pathway
GO:0007193 adenylate cyclase-inhibiting G-protein coupled receptor signaling pathway
GO:0007200 phospholipase C-activating G-protein coupled receptor signaling pathway
GO:0007218 neuropeptide signaling pathway
GO:0007268 chemical synaptic transmission
GO:0007600 sensory perception
GO:0007610 behavior
GO:0007626 locomotory behavior
GO:0009314 response to radiation
GO:0019233 sensory perception of pain
GO:0031635 adenylate cyclase-inhibiting opioid receptor signaling pathway
GO:0032868 response to insulin
GO:0033603 positive regulation of dopamine secretion
GO:0033685 negative regulation of luteinizing hormone secretion
GO:0038003 opioid receptor signaling pathway
GO:0040017 positive regulation of locomotion
GO:0042220 response to cocaine
GO:0042711 maternal behavior
GO:0042755 eating behavior
GO:0043278 response to morphine
GO:0043627 response to estrogen
GO:0044849 estrous cycle
GO:0045471 response to ethanol
GO:0046877 regulation of saliva secretion
GO:0048148 behavioral response to cocaine
GO:0050951 sensory perception of temperature stimulus
GO:0051607 defense response to virus
GO:0051930 regulation of sensory perception of pain
GO:0071222 cellular response to lipopolysaccharide
GO:0071333 cellular response to glucose stimulus
GO:1900745 positive regulation of p38MAPK cascade
GO:1901381 positive regulation of potassium ion transmembrane transport
GO:1903937 response to acrylamide
GO:1904000 positive regulation of eating behavior
GO:1990708 conditioned place preference

Cellular Component:
GO:0005634 nucleus
GO:0005829 cytosol
GO:0005886 plasma membrane
GO:0005887 integral component of plasma membrane
GO:0016020 membrane
GO:0016021 integral component of membrane
GO:0030425 dendrite
GO:0043005 neuron projection
GO:0043025 neuronal cell body
GO:0043204 perikaryon
GO:0043679 axon terminus
GO:0045202 synapse


-  Descriptions from all associated GenBank mRNAs
  AK023198 - Homo sapiens cDNA FLJ13136 fis, clone NT2RP3003139.
BC099912 - Homo sapiens opioid receptor, kappa 1, mRNA (cDNA clone MGC:104656 IMAGE:30531690), complete cds.
JD274638 - Sequence 255662 from Patent EP1572962.
AK310407 - Homo sapiens cDNA, FLJ17449.
AK310233 - Homo sapiens cDNA, FLJ17275.
L37362 - Homo sapiens (clone d2-115) kappa opioid receptor (OPRK1) mRNA, complete cds.
AY168006 - Homo sapiens DRG kappa 1 splice variant KOR 1A mRNA, complete cds, alternatively spliced.
U11053 - Human kappa opioid receptor (hKOR) mRNA, complete cds.
U17298 - Human kappa opioid receptor (hKOR) mRNA, complete cds.
AF498922 - Homo sapiens opioid receptor kappa (OPRK1) mRNA, complete cds.
EF057067 - Homo sapiens kappa opioid receptor 1 variant-like mRNA, complete sequence.
AK313058 - Homo sapiens cDNA, FLJ93535, Homo sapiens opioid receptor, kappa 1 (OPRK1), mRNA.
KJ897279 - Synthetic construct Homo sapiens clone ccsbBroadEn_06673 OPRK1 gene, encodes complete protein.
AB528953 - Synthetic construct DNA, clone: pF1KB6591, Homo sapiens OPRK1 gene for opioid receptor, kappa 1, without stop codon, in Flexi system.
L36130 - Homo sapiens kappa opiate receptor mRNA, partial cds.
AY466378 - Homo sapiens kappa opioid receptor (OPRK1) mRNA, 5' UTR and partial cds.
MN308224 - Homo sapiens isolate KOR-TV6 opioid receptor kappa 1 (OPRK1) mRNA, partial cds, alternatively spliced.
MN308222 - Homo sapiens isolate KOR-TV5A opioid receptor kappa 1 (OPRK1) mRNA, partial cds, alternatively spliced.
MN308223 - Homo sapiens isolate KOR-TV5B opioid receptor kappa 1 (OPRK1) mRNA, partial cds, alternatively spliced.

-  Biochemical and Signaling Pathways
  KEGG - Kyoto Encyclopedia of Genes and Genomes
hsa04080 - Neuroactive ligand-receptor interaction

BioCarta from NCI Cancer Genome Anatomy Project
h_dreampathway - Repression of Pain Sensation by the Transcriptional Regulator DREAM

Reactome (by CSHL, EBI, and GO)

Protein P41145 (Reactome details) participates in the following event(s):

R-HSA-374298 Opioid receptors bind opioid peptides
R-HSA-749454 The Ligand:GPCR:Gi complex dissociates
R-HSA-749456 Liganded Gi-activating GPCRs bind inactive heterotrimeric G-protein Gi
R-HSA-380073 Liganded Gi-activating GPCR acts as a GEF for Gi
R-HSA-375276 Peptide ligand-binding receptors
R-HSA-418594 G alpha (i) signalling events
R-HSA-373076 Class A/1 (Rhodopsin-like receptors)
R-HSA-388396 GPCR downstream signalling
R-HSA-500792 GPCR ligand binding
R-HSA-372790 Signaling by GPCR
R-HSA-162582 Signal Transduction

-  Other Names for This Gene
  Alternate Gene Symbols: ENST00000265572.1, ENST00000265572.2, ENST00000265572.3, ENST00000265572.4, ENST00000265572.5, ENST00000265572.6, ENST00000265572.7, NM_000912, OPRK, OPRK_HUMAN, P41145, Q499G4, uc003xri.1, uc003xri.2, uc003xri.3, uc003xri.4
UCSC ID: ENST00000265572.8
RefSeq Accession: NM_000912
Protein: P41145 (aka OPRK_HUMAN)
CCDS: CCDS6152.1

-  Methods, Credits, and Use Restrictions
  Click here for details on how this gene model was made and data restrictions if any.