Human Gene ADCY8 (ENST00000286355.10) from GENCODE V44
  Description: Homo sapiens adenylate cyclase 8 (ADCY8), mRNA. (from RefSeq NM_001115)
RefSeq Summary (NM_001115): Adenylate cyclase is a membrane bound enzyme that catalyses the formation of cyclic AMP from ATP. The enzymatic activity is under the control of several hormones, and different polypeptides participate in the transduction of the signal from the receptor to the catalytic moiety. Stimulatory or inhibitory receptors (Rs and Ri) interact with G proteins (Gs and Gi) that exhibit GTPase activity and they modulate the activity of the catalytic subunit of the adenylyl cyclase [provided by RefSeq, Jul 2008].
Gencode Transcript: ENST00000286355.10
Gencode Gene: ENSG00000155897.10
Transcript (Including UTRs)
   Position: hg38 chr8:130,780,301-131,040,909 Size: 260,609 Total Exon Count: 18 Strand: -
Coding Region
   Position: hg38 chr8:130,780,390-131,040,333 Size: 259,944 Coding Exon Count: 18 

Page IndexSequence and LinksUniProtKB CommentsPrimersMalaCardsCTD
RNA-Seq ExpressionMicroarray ExpressionRNA StructureProtein StructureOther SpeciesGO Annotations
mRNA DescriptionsPathwaysOther NamesMethods
Data last updated at UCSC: 2023-08-18 00:09:47

-  Sequence and Links to Tools and Databases
Genomic Sequence (chr8:130,780,301-131,040,909)mRNA (may differ from genome)Protein (1251 aa)
Gene SorterGenome BrowserOther Species FASTAGene interactionsTable SchemaAlphaFold
BioGPSEnsemblEntrez GeneExonPrimerGencodeGeneCards
OMIMPubMedReactomeUniProtKBWikipediaBioGrid CRISPR DB

-  Comments and Description Text from UniProtKB
DESCRIPTION: RecName: Full=Adenylate cyclase type 8; EC=; AltName: Full=ATP pyrophosphate-lyase 8; AltName: Full=Adenylate cyclase type VIII; AltName: Full=Adenylyl cyclase 8; Short=AC8; AltName: Full=Ca(2+)/calmodulin-activated adenylyl cyclase;
FUNCTION: This is a membrane-bound, calcium-stimulable adenylyl cyclase. May be involved in learning, in memory and in drug dependence (By similarity).
CATALYTIC ACTIVITY: ATP = 3',5'-cyclic AMP + diphosphate.
COFACTOR: Binds 2 magnesium ions per subunit (By similarity).
ENZYME REGULATION: Activated by calcium/calmodulin.
SUBCELLULAR LOCATION: Membrane; Multi-pass membrane protein.
SIMILARITY: Belongs to the adenylyl cyclase class-4/guanylyl cyclase family.
SIMILARITY: Contains 2 guanylate cyclase domains.

-  Primer design for this transcript

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Click here to load the transcript sequence and exon structure into Primer3Plus

Exonprimer can design one pair of Sanger sequencing primers around every exon, located in non-genic sequence.
Click here to open Exonprimer with this transcript

To design primers for a non-coding sequence, zoom to a region of interest and select from the drop-down menu: View > In External Tools > Primer3

-  MalaCards Disease Associations
  MalaCards Gene Search: ADCY8
Diseases sorted by gene-association score: wolfram syndrome (7)

-  Comparative Toxicogenomics Database (CTD)
  The following chemicals interact with this gene           more ... click here to view the complete list

-  RNA-Seq Expression Data from GTEx (53 Tissues, 570 Donors)
  Highest median expression: 3.28 RPKM in Brain - Amygdala
Total median expression: 37.82 RPKM

View in GTEx track of Genome Browser    View at GTEx portal     View GTEx Body Map

+  Microarray Expression Data
  Press "+" in the title bar above to open this section.

-  mRNA Secondary Structure of 3' and 5' UTRs
RegionFold EnergyBasesEnergy/Base
Display As
5' UTR -254.80576-0.442 Picture PostScript Text
3' UTR -6.1089-0.069 Picture PostScript Text

The RNAfold program from the Vienna RNA Package is used to perform the secondary structure predictions and folding calculations. The estimated folding energy is in kcal/mol. The more negative the energy, the more secondary structure the RNA is likely to have.

-  Protein Domain and Structure Information
  InterPro Domains: Graphical view of domain structure
IPR001054 - A/G_cyclase
IPR018297 - A/G_cyclase_CS
IPR009398 - Adenylate_cyclase-like

Pfam Domains:
PF06327 - Domain of Unknown Function (DUF1053)
PF00211 - Adenylate and Guanylate cyclase catalytic domain

ModBase Predicted Comparative 3D Structure on P40145
The pictures above may be empty if there is no ModBase structure for the protein. The ModBase structure frequently covers just a fragment of the protein. You may be asked to log onto ModBase the first time you click on the pictures. It is simplest after logging in to just click on the picture again to get to the specific info on that model.

-  Orthologous Genes in Other Species
  Orthologies between human, mouse, and rat are computed by taking the best BLASTP hit, and filtering out non-syntenic hits. For more distant species reciprocal-best BLASTP hits are used. Note that the absence of an ortholog in the table below may reflect incomplete annotations in the other species rather than a true absence of the orthologous gene.
MouseRatZebrafishD. melanogasterC. elegansS. cerevisiae
Genome BrowserGenome BrowserGenome BrowserNo orthologNo orthologNo ortholog
Gene Details     
Gene Sorter     
Protein SequenceProtein SequenceProtein Sequence   

-  Gene Ontology (GO) Annotations with Structured Vocabulary
  Molecular Function:
GO:0000166 nucleotide binding
GO:0004016 adenylate cyclase activity
GO:0004383 guanylate cyclase activity
GO:0005524 ATP binding
GO:0008294 calcium- and calmodulin-responsive adenylate cyclase activity
GO:0016829 lyase activity
GO:0016849 phosphorus-oxygen lyase activity
GO:0046872 metal ion binding

Biological Process:
GO:0003091 renal water homeostasis
GO:0006171 cAMP biosynthetic process
GO:0006182 cGMP biosynthetic process
GO:0007165 signal transduction
GO:0007186 G-protein coupled receptor signaling pathway
GO:0007189 adenylate cyclase-activating G-protein coupled receptor signaling pathway
GO:0007193 adenylate cyclase-inhibiting G-protein coupled receptor signaling pathway
GO:0007611 learning or memory
GO:0007616 long-term memory
GO:0009190 cyclic nucleotide biosynthetic process
GO:0035556 intracellular signal transduction
GO:0071377 cellular response to glucagon stimulus

Cellular Component:
GO:0005886 plasma membrane
GO:0008074 guanylate cyclase complex, soluble
GO:0016020 membrane
GO:0016021 integral component of membrane

-  Descriptions from all associated GenBank mRNAs
  GQ472223 - Homo sapiens epididymis secretory sperm binding protein Li 172mP (HEL-S-172mP) mRNA, complete cds.
Z35309 - H.sapiens mRNA for adenylyl cyclase.
M83533 - Homo sapiens adenylyl cyclase type V mRNA, partial cds.
DQ104739 - Homo sapiens brain adenylate cyclase 8 (ADCY8) mRNA, complete cds, alternatively spliced.
BC111456 - Synthetic construct Homo sapiens clone IMAGE:40080646, MGC:133434 ADCY8 protein (ADCY8) mRNA, encodes complete protein.
AB462937 - Synthetic construct DNA, clone: pF1KB7287, Homo sapiens ADCY8 gene for adenylate cyclase 8, without stop codon, in Flexi system.
JD451914 - Sequence 432938 from Patent EP1572962.
JD193653 - Sequence 174677 from Patent EP1572962.
JD246182 - Sequence 227206 from Patent EP1572962.
JD063035 - Sequence 44059 from Patent EP1572962.
JD458602 - Sequence 439626 from Patent EP1572962.

-  Biochemical and Signaling Pathways
  KEGG - Kyoto Encyclopedia of Genes and Genomes
hsa00230 - Purine metabolism
hsa04020 - Calcium signaling pathway
hsa04062 - Chemokine signaling pathway
hsa04114 - Oocyte meiosis
hsa04270 - Vascular smooth muscle contraction
hsa04540 - Gap junction
hsa04720 - Long-term potentiation
hsa04742 - Taste transduction
hsa04912 - GnRH signaling pathway
hsa04914 - Progesterone-mediated oocyte maturation
hsa04916 - Melanogenesis
hsa05414 - Dilated cardiomyopathy

Reactome (by CSHL, EBI, and GO)

Protein P40145 (Reactome details) participates in the following event(s):

R-HSA-381704 G(s):GTP activates Adenylyl cyclase
R-HSA-163617 G alpha (s) activates adenylate cyclase
R-HSA-170672 Galpha-olf:GTP binds to adenylate cyclase and activates it
R-HSA-392064 G alpha (z) inhibits adenylate cyclase
R-HSA-392206 G alpha (i) inhibits adenylate cyclase
R-HSA-170674 Dissociation of the Gi alpha:G olf complex
R-HSA-170677 The G alpha-olf:GDP:Adenylate cyclase complex dissociates
R-HSA-442715 Activation of Adenylate Cyclase
R-HSA-170685 Adenylaye cyclase increases the GTPase activity of G alpha-olf
R-HSA-170686 Adenylate cyclase increases the GTPase activity of Gi alpha
R-HSA-170671 G alpha-olf:GTP binds to Gi alpha1:GTP:adenylate cyclase complex
R-HSA-111930 Adenylate cyclase produces cAMP
R-HSA-392129 Adenylate cyclase converts ATP to 3',5'-cyclic AMP (cAMP) and pyrophosphate
R-HSA-381607 Activated Adenylyl cyclase synthesizes cyclic AMP
R-HSA-5610727 GPR161 promotes cAMP production in a G alpha(s)-dependent manner
R-HSA-164377 Activated Adenylate cyclase catalyses cAMP synthesis
R-HSA-170676 Adenylate cyclase converts ATP into cyclic AMP
R-HSA-170666 Adenylate cyclase increases the GTPase activity of G alpha-olf
R-HSA-163359 Glucagon signaling in metabolic regulation
R-HSA-381676 Glucagon-like Peptide-1 (GLP1) regulates insulin secretion
R-HSA-418555 G alpha (s) signalling events
R-HSA-432040 Vasopressin regulates renal water homeostasis via Aquaporins
R-HSA-170660 Adenylate cyclase activating pathway
R-HSA-418597 G alpha (z) signalling events
R-HSA-170670 Adenylate cyclase inhibitory pathway
R-HSA-418594 G alpha (i) signalling events
R-HSA-442720 CREB phosphorylation through the activation of Adenylate Cyclase
R-HSA-163685 Energy Metabolism
R-HSA-422356 Regulation of insulin secretion
R-HSA-388396 GPCR downstream signalling
R-HSA-445717 Aquaporin-mediated transport
R-HSA-112040 G-protein mediated events
R-HSA-997269 Inhibition of adenylate cyclase pathway
R-HSA-163615 PKA activation
R-HSA-438064 Post NMDA receptor activation events
R-HSA-1430728 Metabolism
R-HSA-5610787 Hedgehog 'off' state
R-HSA-164378 PKA activation in glucagon signalling
R-HSA-372790 Signaling by GPCR
R-HSA-382551 Transport of small molecules
R-HSA-111885 Opioid Signalling
R-HSA-991365 Activation of GABAB receptors
R-HSA-111931 PKA-mediated phosphorylation of CREB
R-HSA-442755 Activation of NMDA receptor and postsynaptic events
R-HSA-5358351 Signaling by Hedgehog
R-HSA-162582 Signal Transduction
R-HSA-977444 GABA B receptor activation
R-HSA-111933 Calmodulin induced events
R-HSA-112314 Neurotransmitter receptors and postsynaptic signal transmission
R-HSA-977443 GABA receptor activation
R-HSA-111997 CaM pathway
R-HSA-112315 Transmission across Chemical Synapses
R-HSA-111996 Ca-dependent events
R-HSA-1489509 DAG and IP3 signaling
R-HSA-112316 Neuronal System
R-HSA-112043 PLC beta mediated events
R-HSA-9006925 Intracellular signaling by second messengers

-  Other Names for This Gene
  Alternate Gene Symbols: ADCY8_HUMAN, ENST00000286355.1, ENST00000286355.2, ENST00000286355.3, ENST00000286355.4, ENST00000286355.5, ENST00000286355.6, ENST00000286355.7, ENST00000286355.8, ENST00000286355.9, NM_001115, P40145, uc003ytd.1, uc003ytd.2, uc003ytd.3, uc003ytd.4, uc003ytd.5, uc003ytd.6
UCSC ID: ENST00000286355.10
RefSeq Accession: NM_001115
Protein: P40145 (aka ADCY8_HUMAN or CYA8_HUMAN)
CCDS: CCDS6363.1

-  Methods, Credits, and Use Restrictions
  Click here for details on how this gene model was made and data restrictions if any.