Human Gene GADD45G (ENST00000252506.11) from GENCODE V44
  Description: Homo sapiens growth arrest and DNA damage inducible gamma (GADD45G), mRNA. (from RefSeq NM_006705)
RefSeq Summary (NM_006705): This gene is a member of a group of genes whose transcript levels are increased following stressful growth arrest conditions and treatment with DNA-damaging agents. The protein encoded by this gene responds to environmental stresses by mediating activation of the p38/JNK pathway via MTK1/MEKK4 kinase. The GADD45G is highly expressed in placenta. [provided by RefSeq, Jul 2008].
Gencode Transcript: ENST00000252506.11
Gencode Gene: ENSG00000130222.11
Transcript (Including UTRs)
   Position: hg38 chr9:89,605,012-89,606,555 Size: 1,544 Total Exon Count: 4 Strand: +
Coding Region
   Position: hg38 chr9:89,605,122-89,606,079 Size: 958 Coding Exon Count: 4 

Page IndexSequence and LinksUniProtKB CommentsPrimersMalaCardsCTD
RNA-Seq ExpressionMicroarray ExpressionRNA StructureProtein StructureOther SpeciesGO Annotations
mRNA DescriptionsPathwaysOther NamesMethods
Data last updated at UCSC: 2023-08-18 00:09:47

-  Sequence and Links to Tools and Databases
 
Genomic Sequence (chr9:89,605,012-89,606,555)mRNA (may differ from genome)Protein (159 aa)
Gene SorterGenome BrowserOther Species FASTAGene interactionsTable SchemaAlphaFold
BioGPSEnsemblEntrez GeneExonPrimerGencodeGeneCards
HGNCHPRDLynxMalacardsMGIneXtProt
OMIMPubMedUniProtKBWikipediaBioGrid CRISPR DB

-  Comments and Description Text from UniProtKB
  ID: GA45G_HUMAN
DESCRIPTION: RecName: Full=Growth arrest and DNA damage-inducible protein GADD45 gamma; AltName: Full=Cytokine-responsive protein CR6; AltName: Full=DNA damage-inducible transcript 2 protein; Short=DDIT-2;
FUNCTION: Involved in the regulation of growth and apoptosis. Mediates activation of stress-responsive MTK1/MEKK4 MAPKKK.
SUBUNIT: Interacts with GADD45GIP1.
INTERACTION: Q8TAE8:GADD45GIP1; NbExp=4; IntAct=EBI-448202, EBI-372506; Q92993:KAT5; NbExp=2; IntAct=EBI-448202, EBI-399080; Q9P2H0:KIAA1377; NbExp=2; IntAct=EBI-448202, EBI-473176; Q9Y3C7:MED31; NbExp=2; IntAct=EBI-448202, EBI-394707; Q9BVJ6:UTP14A; NbExp=2; IntAct=EBI-448202, EBI-473284;
SIMILARITY: Belongs to the GADD45 family.
WEB RESOURCE: Name=NIEHS-SNPs; URL="http://egp.gs.washington.edu/data/gadd45g/";
WEB RESOURCE: Name=Wikipedia; Note=GADD45G entry; URL="http://en.wikipedia.org/wiki/GADD45G";

-  Primer design for this transcript
 

Primer3Plus can design qPCR Primers that straddle exon-exon-junctions, which amplify only cDNA, not genomic DNA.
Click here to load the transcript sequence and exon structure into Primer3Plus

Exonprimer can design one pair of Sanger sequencing primers around every exon, located in non-genic sequence.
Click here to open Exonprimer with this transcript

To design primers for a non-coding sequence, zoom to a region of interest and select from the drop-down menu: View > In External Tools > Primer3


-  MalaCards Disease Associations
  MalaCards Gene Search: GADD45G
Diseases sorted by gene-association score: lung cancer (3)

-  Comparative Toxicogenomics Database (CTD)
  The following chemicals interact with this gene           more ... click here to view the complete list

-  RNA-Seq Expression Data from GTEx (53 Tissues, 570 Donors)
  Highest median expression: 74.61 RPKM in Heart - Atrial Appendage
Total median expression: 1037.03 RPKM



View in GTEx track of Genome Browser    View at GTEx portal     View GTEx Body Map

+  Microarray Expression Data
  Press "+" in the title bar above to open this section.

-  mRNA Secondary Structure of 3' and 5' UTRs
 
RegionFold EnergyBasesEnergy/Base
Display As
5' UTR -38.20110-0.347 Picture PostScript Text
3' UTR -204.80476-0.430 Picture PostScript Text

The RNAfold program from the Vienna RNA Package is used to perform the secondary structure predictions and folding calculations. The estimated folding energy is in kcal/mol. The more negative the energy, the more secondary structure the RNA is likely to have.

-  Protein Domain and Structure Information
  InterPro Domains: Graphical view of domain structure
IPR024824 - GADD45
IPR004038 - Ribosomal_L7Ae/L30e/S12e/Gad45

Pfam Domains:
PF01248 - Ribosomal protein L7Ae/L30e/S12e/Gadd45 family

Protein Data Bank (PDB) 3-D Structure
MuPIT help
2WAL - X-ray MuPIT 3FFM - X-ray MuPIT


ModBase Predicted Comparative 3D Structure on O95257
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-  Orthologous Genes in Other Species
  Orthologies between human, mouse, and rat are computed by taking the best BLASTP hit, and filtering out non-syntenic hits. For more distant species reciprocal-best BLASTP hits are used. Note that the absence of an ortholog in the table below may reflect incomplete annotations in the other species rather than a true absence of the orthologous gene.
MouseRatZebrafishD. melanogasterC. elegansS. cerevisiae
Genome BrowserGenome BrowserNo orthologNo orthologNo orthologNo ortholog
Gene Details     
Gene Sorter     
MGIRGDEnsembl   
Protein SequenceProtein SequenceProtein Sequence   
AlignmentAlignmentAlignment   

-  Gene Ontology (GO) Annotations with Structured Vocabulary
  Molecular Function:
GO:0005515 protein binding

Biological Process:
GO:0000185 activation of MAPKKK activity
GO:0006915 apoptotic process
GO:0006950 response to stress
GO:0007275 multicellular organism development
GO:0030154 cell differentiation
GO:0043065 positive regulation of apoptotic process
GO:0046330 positive regulation of JNK cascade
GO:0051726 regulation of cell cycle
GO:1900745 positive regulation of p38MAPK cascade

Cellular Component:
GO:0005634 nucleus
GO:0005737 cytoplasm


-  Descriptions from all associated GenBank mRNAs
  AY845250 - Homo sapiens growth arrest and DNA-damage-inducible gamma (GADD45G) mRNA, 5' UTR and partial cds.
AK313689 - Homo sapiens cDNA, FLJ94278, Homo sapiens growth arrest and DNA-damage-inducible, gamma(GADD45G), mRNA.
AF079806 - Homo sapiens cytokine responsive protein (CR6) mRNA, complete cds.
AF087883 - Homo sapiens growth arrest and DNA damage inducible protein gamma (GADD45G) mRNA, complete cds.
BC000465 - Homo sapiens growth arrest and DNA-damage-inducible, gamma, mRNA (cDNA clone MGC:8544 IMAGE:2822765), complete cds.
BC019325 - Homo sapiens growth arrest and DNA-damage-inducible, gamma, mRNA (cDNA clone MGC:4374 IMAGE:2823131), complete cds.
D83023 - Homo sapiens GRP17 mRNA for gadd45-related protein, complete cds.
JD390609 - Sequence 371633 from Patent EP1572962.
AF078078 - Homo sapiens growth arrest and DNA-damage-inducible protein GADD45gamma mRNA, complete cds.
CR457038 - Homo sapiens full open reading frame cDNA clone RZPDo834H027D for gene GADD45G, growth arrest and DNA-damage-inducible, gamma; complete cds, incl. stopcodon.
AB463699 - Synthetic construct DNA, clone: pF1KB6803, Homo sapiens GADD45G gene for growth arrest and DNA-damage-inducible, gamma, without stop codon, in Flexi system.
BT007234 - Homo sapiens growth arrest and DNA-damage-inducible, gamma mRNA, complete cds.
KJ893163 - Synthetic construct Homo sapiens clone ccsbBroadEn_02557 GADD45G gene, encodes complete protein.
JD391698 - Sequence 372722 from Patent EP1572962.
JD192149 - Sequence 173173 from Patent EP1572962.
JD197295 - Sequence 178319 from Patent EP1572962.
JD459931 - Sequence 440955 from Patent EP1572962.
JD044243 - Sequence 25267 from Patent EP1572962.
JD327306 - Sequence 308330 from Patent EP1572962.

-  Biochemical and Signaling Pathways
  KEGG - Kyoto Encyclopedia of Genes and Genomes
hsa04010 - MAPK signaling pathway
hsa04110 - Cell cycle
hsa04115 - p53 signaling pathway

-  Other Names for This Gene
  Alternate Gene Symbols: CR6, DDIT2, ENST00000252506.1, ENST00000252506.10, ENST00000252506.2, ENST00000252506.3, ENST00000252506.4, ENST00000252506.5, ENST00000252506.6, ENST00000252506.7, ENST00000252506.8, ENST00000252506.9, GA45G_HUMAN, NM_006705, O95257, Q9C076, uc004aqq.1, uc004aqq.2, uc004aqq.3, uc004aqq.4, uc004aqq.5
UCSC ID: ENST00000252506.11
RefSeq Accession: NM_006705
Protein: O95257 (aka GA45G_HUMAN or G45G_HUMAN)
CCDS: CCDS6686.1

-  Methods, Credits, and Use Restrictions
  Click here for details on how this gene model was made and data restrictions if any.