Human Gene PRPS2 (ENST00000380668.10) from GENCODE V44
  Description: Homo sapiens phosphoribosyl pyrophosphate synthetase 2 (PRPS2), transcript variant 2, mRNA. (from RefSeq NM_002765)
RefSeq Summary (NM_002765): This gene encodes a phosphoribosyl pyrophosphate synthetase that plays a central role in the synthesis of purines and pyrimidines. The encoded protein catalyzes the synthesis of 5-phosphoribosyl 1-pyrophosphate from ATP and D-ribose 5-phosphate. Alternate splicing results in multiple transcript variants. [provided by RefSeq, Mar 2010].
Gencode Transcript: ENST00000380668.10
Gencode Gene: ENSG00000101911.13
Transcript (Including UTRs)
   Position: hg38 chrX:12,791,412-12,824,222 Size: 32,811 Total Exon Count: 7 Strand: +
Coding Region
   Position: hg38 chrX:12,791,498-12,822,796 Size: 31,299 Coding Exon Count: 7 

Page IndexSequence and LinksUniProtKB CommentsPrimersCTDRNA-Seq Expression
Microarray ExpressionRNA StructureProtein StructureOther SpeciesGO AnnotationsmRNA Descriptions
PathwaysOther NamesMethods
Data last updated at UCSC: 2023-08-18 00:09:47

-  Sequence and Links to Tools and Databases
 
Genomic Sequence (chrX:12,791,412-12,824,222)mRNA (may differ from genome)Protein (318 aa)
Gene SorterGenome BrowserOther Species FASTAGene interactionsTable SchemaAlphaFold
BioGPSEnsemblEntrez GeneExonPrimerGencodeGeneCards
HGNCHPRDLynxMalacardsMGIneXtProt
OMIMPubMedReactomeUniProtKBBioGrid CRISPR DB

-  Comments and Description Text from UniProtKB
  ID: PRPS2_HUMAN
DESCRIPTION: RecName: Full=Ribose-phosphate pyrophosphokinase 2; EC=2.7.6.1; AltName: Full=PPRibP; AltName: Full=Phosphoribosyl pyrophosphate synthase II; Short=PRS-II;
FUNCTION: Catalyzes the synthesis of phosphoribosylpyrophosphate (PRPP) that is essential for nucleotide synthesis.
CATALYTIC ACTIVITY: ATP + D-ribose 5-phosphate = AMP + 5-phospho- alpha-D-ribose 1-diphosphate.
COFACTOR: Magnesium.
ENZYME REGULATION: Activated by magnesium and inorganic phosphate. Competitively or non-competitively inhibited by ADP, 2,3- bisphosphoglyceride or GDP.
PATHWAY: Metabolic intermediate biosynthesis; 5-phospho-alpha-D- ribose 1-diphosphate biosynthesis; 5-phospho-alpha-D-ribose 1- diphosphate from D-ribose 5-phosphate (route I): step 1/1.
SUBUNIT: Homodimer. The active form is probably a hexamer composed of 3 homodimers (By similarity).
SIMILARITY: Belongs to the ribose-phosphate pyrophosphokinase family.
SEQUENCE CAUTION: Sequence=AAH30019.2; Type=Erroneous initiation;

-  Primer design for this transcript
 

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Click here to load the transcript sequence and exon structure into Primer3Plus

Exonprimer can design one pair of Sanger sequencing primers around every exon, located in non-genic sequence.
Click here to open Exonprimer with this transcript

To design primers for a non-coding sequence, zoom to a region of interest and select from the drop-down menu: View > In External Tools > Primer3


-  Comparative Toxicogenomics Database (CTD)
  The following chemicals interact with this gene           more ... click here to view the complete list

-  RNA-Seq Expression Data from GTEx (53 Tissues, 570 Donors)
  Highest median expression: 69.09 RPKM in Testis
Total median expression: 609.10 RPKM



View in GTEx track of Genome Browser    View at GTEx portal     View GTEx Body Map

+  Microarray Expression Data
  Press "+" in the title bar above to open this section.

-  mRNA Secondary Structure of 3' and 5' UTRs
 
RegionFold EnergyBasesEnergy/Base
Display As
5' UTR -23.4086-0.272 Picture PostScript Text
3' UTR -326.301426-0.229 Picture PostScript Text

The RNAfold program from the Vienna RNA Package is used to perform the secondary structure predictions and folding calculations. The estimated folding energy is in kcal/mol. The more negative the energy, the more secondary structure the RNA is likely to have.

-  Protein Domain and Structure Information
  InterPro Domains: Graphical view of domain structure
IPR000842 - PRib_PP_synth_CS
IPR000836 - PRibTrfase_dom
IPR005946 - Rib-P_diPkinase

Pfam Domains:
PF00156 - Phosphoribosyl transferase domain

ModBase Predicted Comparative 3D Structure on P11908
FrontTopSide
The pictures above may be empty if there is no ModBase structure for the protein. The ModBase structure frequently covers just a fragment of the protein. You may be asked to log onto ModBase the first time you click on the pictures. It is simplest after logging in to just click on the picture again to get to the specific info on that model.

-  Orthologous Genes in Other Species
  Orthologies between human, mouse, and rat are computed by taking the best BLASTP hit, and filtering out non-syntenic hits. For more distant species reciprocal-best BLASTP hits are used. Note that the absence of an ortholog in the table below may reflect incomplete annotations in the other species rather than a true absence of the orthologous gene.
MouseRatZebrafishD. melanogasterC. elegansS. cerevisiae
Genome BrowserGenome BrowserNo orthologNo orthologGenome BrowserGenome Browser
Gene Details    Gene Details
Gene Sorter    Gene Sorter
MGIRGD  WormBaseSGD
Protein SequenceProtein Sequence  Protein SequenceProtein Sequence
AlignmentAlignment  AlignmentAlignment

-  Gene Ontology (GO) Annotations with Structured Vocabulary
  Molecular Function:
GO:0000166 nucleotide binding
GO:0000287 magnesium ion binding
GO:0004749 ribose phosphate diphosphokinase activity
GO:0005515 protein binding
GO:0005524 ATP binding
GO:0016208 AMP binding
GO:0016301 kinase activity
GO:0016740 transferase activity
GO:0019003 GDP binding
GO:0030246 carbohydrate binding
GO:0042802 identical protein binding
GO:0042803 protein homodimerization activity
GO:0043531 ADP binding
GO:0046872 metal ion binding

Biological Process:
GO:0006015 5-phosphoribose 1-diphosphate biosynthetic process
GO:0006139 nucleobase-containing compound metabolic process
GO:0006167 AMP biosynthetic process
GO:0009116 nucleoside metabolic process
GO:0009156 ribonucleoside monophosphate biosynthetic process
GO:0009165 nucleotide biosynthetic process
GO:0016310 phosphorylation
GO:0019693 ribose phosphate metabolic process
GO:0031100 animal organ regeneration
GO:0044249 cellular biosynthetic process

Cellular Component:
GO:0002189 ribose phosphate diphosphokinase complex
GO:0032991 macromolecular complex


-  Descriptions from all associated GenBank mRNAs
  HM005581 - Homo sapiens clone HTL-S-41 testicular secretory protein Li 41 mRNA, complete cds.
AK313246 - Homo sapiens cDNA, FLJ93752, highly similar to Homo sapiens phosphoribosyl pyrophosphate synthetase 2 (PRPS2), mRNA.
BC040483 - Homo sapiens phosphoribosyl pyrophosphate synthetase 2, mRNA (cDNA clone MGC:33134 IMAGE:5272983), complete cds.
BC030019 - Homo sapiens phosphoribosyl pyrophosphate synthetase 2, mRNA (cDNA clone IMAGE:4830609), partial cds.
BC110875 - Homo sapiens phosphoribosyl pyrophosphate synthetase 2, mRNA (cDNA clone MGC:131896 IMAGE:6472836), complete cds.
BC119662 - Homo sapiens phosphoribosyl pyrophosphate synthetase 2, mRNA (cDNA clone MGC:149529 IMAGE:40116022), complete cds.
BC119663 - Homo sapiens phosphoribosyl pyrophosphate synthetase 2, mRNA (cDNA clone MGC:149530 IMAGE:40116023), complete cds.
Y00971 - Human mRNA for phosphoriobosyl pyrophosphate synthetase subunit II (EC 2.7.6.1).
KJ891904 - Synthetic construct Homo sapiens clone ccsbBroadEn_01298 PRPS2 gene, encodes complete protein.
KJ891905 - Synthetic construct Homo sapiens clone ccsbBroadEn_01299 PRPS2 gene, encodes complete protein.
CU691884 - Synthetic construct Homo sapiens gateway clone IMAGE:100020814 5' read PRPS2 mRNA.
KJ897388 - Synthetic construct Homo sapiens clone ccsbBroadEn_06782 PRPS2 gene, encodes complete protein.
KJ905301 - Synthetic construct Homo sapiens clone ccsbBroadEn_14816 PRPS2 gene, encodes complete protein.
AK310332 - Homo sapiens cDNA, FLJ17374.
LF211730 - JP 2014500723-A/19233: Polycomb-Associated Non-Coding RNAs.
MA447307 - JP 2018138019-A/19233: Polycomb-Associated Non-Coding RNAs.
JD309814 - Sequence 290838 from Patent EP1572962.
JD332071 - Sequence 313095 from Patent EP1572962.
JD521960 - Sequence 502984 from Patent EP1572962.
JD065229 - Sequence 46253 from Patent EP1572962.
JD114544 - Sequence 95568 from Patent EP1572962.
JD059173 - Sequence 40197 from Patent EP1572962.
JD419874 - Sequence 400898 from Patent EP1572962.
JD226022 - Sequence 207046 from Patent EP1572962.
JD279738 - Sequence 260762 from Patent EP1572962.

-  Biochemical and Signaling Pathways
  KEGG - Kyoto Encyclopedia of Genes and Genomes
hsa00030 - Pentose phosphate pathway
hsa00230 - Purine metabolism
hsa01100 - Metabolic pathways

BioCyc Knowledge Library
PWY0-662 - PRPP biosynthesis

Reactome (by CSHL, EBI, and GO)

Protein P11908 (Reactome details) participates in the following event(s):

R-HSA-73580 D-ribose 5-phosphate + ATP => 5-phospho-alpha-D-ribose 1-diphosphate (PRPP) + adenosine 5'-monophosphate
R-HSA-73843 5-Phosphoribose 1-diphosphate biosynthesis
R-HSA-71336 Pentose phosphate pathway (hexose monophosphate shunt)
R-HSA-71387 Metabolism of carbohydrates
R-HSA-1430728 Metabolism

-  Other Names for This Gene
  Alternate Gene Symbols: ENST00000380668.1, ENST00000380668.2, ENST00000380668.3, ENST00000380668.4, ENST00000380668.5, ENST00000380668.6, ENST00000380668.7, ENST00000380668.8, ENST00000380668.9, NM_002765, P11908, PRPS2_HUMAN, Q0VDH9, Q0VDI0, Q15245, Q2TAK7, uc004cvb.1, uc004cvb.2, uc004cvb.3, uc004cvb.4, uc004cvb.5
UCSC ID: ENST00000380668.10
RefSeq Accession: NM_002765
Protein: P11908 (aka PRPS2_HUMAN or KPR2_HUMAN)
CCDS: CCDS14150.1, CCDS43918.1

-  Methods, Credits, and Use Restrictions
  Click here for details on how this gene model was made and data restrictions if any.