Human Gene ME3 (ENST00000543262.6) from GENCODE V44
Description: (S)-malate + NADP(+) = pyruvate + CO(2) + NADPH. (from UniProt Q16798) RefSeq Summary (NM_001161586): Malic enzyme catalyzes the oxidative decarboxylation of malate to pyruvate using either NAD+ or NADP+ as a cofactor. Mammalian tissues contain 3 distinct isoforms of malic enzyme: a cytosolic NADP(+)-dependent isoform, a mitochondrial NADP(+)-dependent isoform, and a mitochondrial NAD(+)-dependent isoform. This gene encodes a mitochondrial NADP(+)-dependent isoform. Multiple alternatively spliced transcript variants have been found for this gene, but the biological validity of some variants has not been determined. [provided by RefSeq, Jul 2008]. Gencode Transcript: ENST00000543262.6 Gencode Gene: ENSG00000151376.18 Transcript (Including UTRs) Position: hg38 chr11:86,441,108-86,672,616 Size: 231,509 Total Exon Count: 15 Strand: - Coding Region Position: hg38 chr11:86,441,279-86,671,944 Size: 230,666 Coding Exon Count: 14
The RNAfold program from the Vienna RNA Package is used to perform the secondary structure predictions and folding calculations. The estimated folding energy is in kcal/mol. The more negative the energy, the more secondary structure the RNA is likely to have.
ModBase Predicted Comparative 3D Structure on Q16798
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Orthologous Genes in Other Species
Orthologies between human, mouse, and rat are computed by taking the best BLASTP hit, and filtering out non-syntenic hits. For more distant species reciprocal-best BLASTP hits are used. Note that the absence of an ortholog in the table below may reflect incomplete annotations in the other species rather than a true absence of the orthologous gene.
BC022472 - Homo sapiens malic enzyme 3, NADP(+)-dependent, mitochondrial, mRNA (cDNA clone MGC:26257 IMAGE:4812045), complete cds. X79440 - H.sapiens mRNA for NADP+-dependent malic enzyme. AK313695 - Homo sapiens cDNA, FLJ94285, highly similar to Homo sapiens malic enzyme 3, NADP(+)-dependent, mitochondrial(ME3), nuclear gene encoding mitochondrial protein, mRNA. DQ892380 - Synthetic construct clone IMAGE:100005010; FLH185774.01X; RZPDo839E11148D malic enzyme 3, NADP(+)-dependent, mitochondrial (ME3) gene, encodes complete protein; nuclear gene for mitochondrial product. DQ893285 - Synthetic construct clone IMAGE:100005915; FLH195961.01X; RZPDo839A06154D malic enzyme 3, NADP(+)-dependent, mitochondrial (ME3) gene, encodes complete protein; nuclear gene for mitochondrial product. DQ896617 - Synthetic construct Homo sapiens clone IMAGE:100011077; FLH195957.01L; RZPDo839A06153D malic enzyme 3, NADP(+)-dependent, mitochondrial (ME3) gene, encodes complete protein; nuclear gene for mitochondrial product. AK300974 - Homo sapiens cDNA FLJ58694 complete cds, highly similar to NADP-dependent malic enzyme, mitochondrial precursor (EC 1.1.1.40). AK092181 - Homo sapiens cDNA FLJ34862 fis, clone NT2NE2013547, highly similar to NADP-DEPENDENT MALIC ENZYME, MITOCHONDRIAL PRECURSOR (EC 1.1.1.40). AY424278 - Homo sapiens mitochondrial NADP(+)-dependent malic enzyme 3 (ME3) mRNA, complete cds, alternatively spliced; nuclear gene for mitochondrial product. JD050966 - Sequence 31990 from Patent EP1572962. JD173661 - Sequence 154685 from Patent EP1572962. JD310092 - Sequence 291116 from Patent EP1572962.
Biochemical and Signaling Pathways
BioCyc Knowledge Library MALATE-ASPARTATE-SHUTTLE-PWY - malate-aspartate shuttle PWY66-398 - TCA cycle PWY66-399 - gluconeogenesis PWY66-407 - superpathway of conversion of glucose to acetyl CoA and entry into the TCA cycle
Reactome (by CSHL, EBI, and GO)
Protein Q16798 (Reactome details) participates in the following event(s):
R-HSA-9012349 ME3:Mg2+ tetramer oxidatively decarboxylates MAL to PYR R-HSA-71403 Citric acid cycle (TCA cycle) R-HSA-71406 Pyruvate metabolism and Citric Acid (TCA) cycle R-HSA-1428517 The citric acid (TCA) cycle and respiratory electron transport R-HSA-1430728 Metabolism