Human Gene NOL4 (ENST00000261592.10) from GENCODE V44
  Description: Homo sapiens nucleolar protein 4 (NOL4), transcript variant 1, mRNA. (from RefSeq NM_003787)
Gencode Transcript: ENST00000261592.10
Gencode Gene: ENSG00000101746.16
Transcript (Including UTRs)
   Position: hg38 chr18:33,851,100-34,224,913 Size: 373,814 Total Exon Count: 11 Strand: -
Coding Region
   Position: hg38 chr18:33,852,842-34,223,253 Size: 370,412 Coding Exon Count: 11 

Page IndexSequence and LinksUniProtKB CommentsPrimersCTDRNA-Seq Expression
Microarray ExpressionRNA StructureProtein StructureOther SpeciesGO AnnotationsmRNA Descriptions
Other NamesMethods
Data last updated at UCSC: 2023-08-18 00:09:47

-  Sequence and Links to Tools and Databases
Genomic Sequence (chr18:33,851,100-34,224,913)mRNA (may differ from genome)Protein (638 aa)
Gene SorterGenome BrowserOther Species FASTAGene interactionsTable SchemaAlphaFold
BioGPSEnsemblEntrez GeneExonPrimerGencodeGeneCards
PubMedUniProtKBWikipediaBioGrid CRISPR DB

-  Comments and Description Text from UniProtKB
DESCRIPTION: RecName: Full=Nucleolar protein 4; AltName: Full=Nucleolar-localized protein;
SUBCELLULAR LOCATION: Nucleus, nucleolus.
TISSUE SPECIFICITY: Expressed predominantly in fetal brain, adult brain and testis.
SEQUENCE CAUTION: Sequence=AAH00313.1; Type=Miscellaneous discrepancy; Note=Contaminating sequence. Sequence of unknown origin in the N-terminal part; Sequence=BAA34576.1; Type=Erroneous termination; Positions=38; Note=Translated as Gln; Sequence=BAA34576.1; Type=Frameshift; Positions=82;

-  Primer design for this transcript

Primer3Plus can design qPCR Primers that straddle exon-exon-junctions, which amplify only cDNA, not genomic DNA.
Click here to load the transcript sequence and exon structure into Primer3Plus

Exonprimer can design one pair of Sanger sequencing primers around every exon, located in non-genic sequence.
Click here to open Exonprimer with this transcript

To design primers for a non-coding sequence, zoom to a region of interest and select from the drop-down menu: View > In External Tools > Primer3

-  Comparative Toxicogenomics Database (CTD)
  The following chemicals interact with this gene

-  RNA-Seq Expression Data from GTEx (53 Tissues, 570 Donors)
  Highest median expression: 5.93 RPKM in Brain - Cerebellar Hemisphere
Total median expression: 57.76 RPKM

View in GTEx track of Genome Browser    View at GTEx portal     View GTEx Body Map

+  Microarray Expression Data
  Press "+" in the title bar above to open this section.

-  mRNA Secondary Structure of 3' and 5' UTRs
RegionFold EnergyBasesEnergy/Base
Display As
5' UTR -615.901660-0.371 Picture PostScript Text
3' UTR -383.821742-0.220 Picture PostScript Text

The RNAfold program from the Vienna RNA Package is used to perform the secondary structure predictions and folding calculations. The estimated folding energy is in kcal/mol. The more negative the energy, the more secondary structure the RNA is likely to have.

-  Protein Domain and Structure Information
  InterPro Domains: Graphical view of domain structure
IPR026747 - NOL4

ModBase Predicted Comparative 3D Structure on O94818
The pictures above may be empty if there is no ModBase structure for the protein. The ModBase structure frequently covers just a fragment of the protein. You may be asked to log onto ModBase the first time you click on the pictures. It is simplest after logging in to just click on the picture again to get to the specific info on that model.

-  Orthologous Genes in Other Species
  Orthologies between human, mouse, and rat are computed by taking the best BLASTP hit, and filtering out non-syntenic hits. For more distant species reciprocal-best BLASTP hits are used. Note that the absence of an ortholog in the table below may reflect incomplete annotations in the other species rather than a true absence of the orthologous gene.
MouseRatZebrafishD. melanogasterC. elegansS. cerevisiae
Genome BrowserGenome BrowserNo orthologNo orthologNo orthologNo ortholog
Gene Details     
Gene Sorter     
Protein SequenceProtein Sequence    

-  Gene Ontology (GO) Annotations with Structured Vocabulary
  Molecular Function:
GO:0003723 RNA binding
GO:0005515 protein binding

Cellular Component:
GO:0005634 nucleus
GO:0005730 nucleolus

-  Descriptions from all associated GenBank mRNAs
  AK296539 - Homo sapiens cDNA FLJ54015 complete cds, highly similar to Nucleolar protein 4.
BC000313 - Homo sapiens nucleolar protein 4, mRNA (cDNA clone MGC:8430 IMAGE:2821116), complete cds.
AB017800 - Homo sapiens nolp mRNA, complete cds.
AK091463 - Homo sapiens cDNA FLJ34144 fis, clone FCBBF3011592, highly similar to Homo sapiens nolp mRNA.
AX746999 - Sequence 524 from Patent EP1308459.
AK299850 - Homo sapiens cDNA FLJ56029 complete cds, highly similar to Nucleolar protein 4.
AK297185 - Homo sapiens cDNA FLJ60679 complete cds, highly similar to Nucleolar protein 4.
AB015339 - Homo sapiens HRIHFB2255 mRNA, partial cds.
AK312562 - Homo sapiens cDNA, FLJ92936.
KJ901903 - Synthetic construct Homo sapiens clone ccsbBroadEn_11297 NOL4 gene, encodes complete protein.
BT006763 - Homo sapiens nucleolar protein 4 mRNA, complete cds.
CR456730 - Homo sapiens full open reading frame cDNA clone RZPDo834F0114D for gene NOL4, nucleolar protein 4; complete cds, incl. stopcodon.
CU674152 - Synthetic construct Homo sapiens gateway clone IMAGE:100018248 5' read NOL4 mRNA.
JD360985 - Sequence 342009 from Patent EP1572962.
JD300707 - Sequence 281731 from Patent EP1572962.
JD503279 - Sequence 484303 from Patent EP1572962.
JD062050 - Sequence 43074 from Patent EP1572962.
JD449840 - Sequence 430864 from Patent EP1572962.
JD461884 - Sequence 442908 from Patent EP1572962.
JD392329 - Sequence 373353 from Patent EP1572962.
JD439804 - Sequence 420828 from Patent EP1572962.

-  Other Names for This Gene
  Alternate Gene Symbols: B4DSQ0, ENST00000261592.1, ENST00000261592.2, ENST00000261592.3, ENST00000261592.4, ENST00000261592.5, ENST00000261592.6, ENST00000261592.7, ENST00000261592.8, ENST00000261592.9, HRIHFB2255, NM_003787, NOL4_HUMAN, NOLP, O94818, Q6IBS2, Q9BWF1, uc010dmi.1, uc010dmi.2, uc010dmi.3, uc010dmi.4
UCSC ID: ENST00000261592.10
RefSeq Accession: NM_003787
Protein: O94818 (aka NOL4_HUMAN)

-  Methods, Credits, and Use Restrictions
  Click here for details on how this gene model was made and data restrictions if any.