Human Gene PROKR1 (ENST00000303786.5) from GENCODE V44
  Description: Homo sapiens prokineticin receptor 1 (PROKR1), mRNA. (from RefSeq NM_138964)
RefSeq Summary (NM_138964): This gene encodes a member of the G-protein-coupled receptor family. The encoded protein binds to prokineticins (1 and 2), leading to the activation of MAPK and STAT signaling pathways. Prokineticins are protein ligands involved in angiogenesis and inflammation. The encoded protein is expressed in peripheral tissues such as those comprising the circulatory system, lungs, reproductive system, endocrine system and the gastrointestinal system. The protein may be involved in signaling in human fetal ovary during initiation of primordial follicle formation. Sequence variants in this gene may be associated with recurrent miscarriage. [provided by RefSeq, Aug 2016].
Gencode Transcript: ENST00000303786.5
Gencode Gene: ENSG00000169618.7
Transcript (Including UTRs)
   Position: hg38 chr2:68,643,579-68,658,251 Size: 14,673 Total Exon Count: 3 Strand: +
Coding Region
   Position: hg38 chr2:68,645,822-68,655,576 Size: 9,755 Coding Exon Count: 2 

Page IndexSequence and LinksUniProtKB CommentsPrimersMalaCardsCTD
RNA-Seq ExpressionRNA StructureProtein StructureOther SpeciesGO AnnotationsmRNA Descriptions
PathwaysOther NamesMethods
Data last updated at UCSC: 2023-08-18 00:09:47

-  Sequence and Links to Tools and Databases
 
Genomic Sequence (chr2:68,643,579-68,658,251)mRNA (may differ from genome)Protein (393 aa)
Gene SorterGenome BrowserOther Species FASTAGene interactionsTable SchemaAlphaFold
BioGPSEnsemblEntrez GeneExonPrimerGencodeGeneCards
HGNCHPRDLynxMalacardsMGIneXtProt
OMIMPubMedReactomeUniProtKBWikipediaBioGrid CRISPR DB

-  Comments and Description Text from UniProtKB
  ID: PKR1_HUMAN
DESCRIPTION: RecName: Full=Prokineticin receptor 1; Short=PK-R1; AltName: Full=G-protein coupled receptor 73; AltName: Full=G-protein coupled receptor ZAQ; AltName: Full=GPR73a;
FUNCTION: Receptor for prokineticin 1. Exclusively coupled to the G(q) subclass of heteromeric G proteins. Activation leads to mobilization of calcium, stimulation of phosphoinositide turnover and activation of p44/p42 mitogen-activated protein kinase.
SUBCELLULAR LOCATION: Cell membrane; Multi-pass membrane protein.
TISSUE SPECIFICITY: Expressed in the stomach, throughout the small intestine, colon, rectum, thyroid gland, pituitary gland, salivary gland, adrenal gland, testis, ovary, brain, spleen, prostate and pancreas.
SIMILARITY: Belongs to the G-protein coupled receptor 1 family.

-  Primer design for this transcript
 

Primer3Plus can design qPCR Primers that straddle exon-exon-junctions, which amplify only cDNA, not genomic DNA.
Click here to load the transcript sequence and exon structure into Primer3Plus

Exonprimer can design one pair of Sanger sequencing primers around every exon, located in non-genic sequence.
Click here to open Exonprimer with this transcript

To design primers for a non-coding sequence, zoom to a region of interest and select from the drop-down menu: View > In External Tools > Primer3


-  MalaCards Disease Associations
  MalaCards Gene Search: PROKR1
Diseases sorted by gene-association score: ectopic pregnancy (9)

-  Comparative Toxicogenomics Database (CTD)
  The following chemicals interact with this gene           more ... click here to view the complete list

-  RNA-Seq Expression Data from GTEx (53 Tissues, 570 Donors)
  Highest median expression: 0.95 RPKM in Testis
Total median expression: 2.26 RPKM



View in GTEx track of Genome Browser    View at GTEx portal     View GTEx Body Map

-  mRNA Secondary Structure of 3' and 5' UTRs
 
RegionFold EnergyBasesEnergy/Base
Display As
5' UTR -194.30430-0.452 Picture PostScript Text
3' UTR -793.902675-0.297 Picture PostScript Text

The RNAfold program from the Vienna RNA Package is used to perform the secondary structure predictions and folding calculations. The estimated folding energy is in kcal/mol. The more negative the energy, the more secondary structure the RNA is likely to have.

-  Protein Domain and Structure Information
  InterPro Domains: Graphical view of domain structure
IPR000276 - 7TM_GPCR_Rhodpsn
IPR017452 - GPCR_Rhodpsn_supfam
IPR000611 - NPY_rcpt

Pfam Domains:
PF00001 - 7 transmembrane receptor (rhodopsin family)

ModBase Predicted Comparative 3D Structure on Q8TCW9
FrontTopSide
The pictures above may be empty if there is no ModBase structure for the protein. The ModBase structure frequently covers just a fragment of the protein. You may be asked to log onto ModBase the first time you click on the pictures. It is simplest after logging in to just click on the picture again to get to the specific info on that model.

-  Orthologous Genes in Other Species
  Orthologies between human, mouse, and rat are computed by taking the best BLASTP hit, and filtering out non-syntenic hits. For more distant species reciprocal-best BLASTP hits are used. Note that the absence of an ortholog in the table below may reflect incomplete annotations in the other species rather than a true absence of the orthologous gene.
MouseRatZebrafishD. melanogasterC. elegansS. cerevisiae
Genome BrowserNo orthologGenome BrowserNo orthologNo orthologNo ortholog
Gene Details     
Gene Sorter     
MGI Ensembl   
Protein Sequence Protein Sequence   
Alignment Alignment   

-  Gene Ontology (GO) Annotations with Structured Vocabulary
  Molecular Function:
GO:0001602 pancreatic polypeptide receptor activity
GO:0004871 signal transducer activity
GO:0004930 G-protein coupled receptor activity
GO:0004983 neuropeptide Y receptor activity

Biological Process:
GO:0007165 signal transduction
GO:0007186 G-protein coupled receptor signaling pathway
GO:0007218 neuropeptide signaling pathway
GO:0007268 chemical synaptic transmission
GO:0007623 circadian rhythm
GO:0007631 feeding behavior
GO:0008015 blood circulation
GO:0043066 negative regulation of apoptotic process

Cellular Component:
GO:0005886 plasma membrane
GO:0016020 membrane
GO:0016021 integral component of membrane


-  Descriptions from all associated GenBank mRNAs
  AK122915 - Homo sapiens cDNA FLJ16593 fis, clone TESTI4004722, highly similar to Mus musculus G protein-coupled receptor 73 (Gpr73).
KJ893154 - Synthetic construct Homo sapiens clone ccsbBroadEn_02548 PROKR1 gene, encodes complete protein.
AB084080 - Homo sapiens mRNA for GPR73, complete cds.
AF506287 - Homo sapiens prokineticin receptor 1 (PKR1) mRNA, complete cds.
AY089976 - Homo sapiens G protein-coupled receptor ZAQ mRNA, complete cds.
BC100960 - Homo sapiens prokineticin receptor 1, mRNA (cDNA clone MGC:119220 IMAGE:40004720), complete cds.
EF577399 - Homo sapiens prokineticin receptor 1 mRNA, complete cds.

-  Biochemical and Signaling Pathways
  Reactome (by CSHL, EBI, and GO)

Protein Q8TCW9 (Reactome details) participates in the following event(s):

R-HSA-444691 Prokineticin receptors bind prokineticin
R-HSA-749452 The Ligand:GPCR:Gq complex dissociates
R-HSA-749448 Liganded Gq-activating GPCRs bind inactive heterotrimeric Gq
R-HSA-379048 Liganded Gq/11-activating GPCRs act as GEFs for Gq/11
R-HSA-375276 Peptide ligand-binding receptors
R-HSA-416476 G alpha (q) signalling events
R-HSA-373076 Class A/1 (Rhodopsin-like receptors)
R-HSA-388396 GPCR downstream signalling
R-HSA-500792 GPCR ligand binding
R-HSA-372790 Signaling by GPCR
R-HSA-162582 Signal Transduction

-  Other Names for This Gene
  Alternate Gene Symbols: A5JUU2, ENST00000303786.1, ENST00000303786.2, ENST00000303786.3, ENST00000303786.4, GPR73, NM_138964, PKR1, PKR1_HUMAN, Q53QT9, Q8NFJ7, Q8TCW9, uc010yqj.1, uc010yqj.2, uc010yqj.3, uc010yqj.4
UCSC ID: ENST00000303786.5
RefSeq Accession: NM_138964
Protein: Q8TCW9 (aka PKR1_HUMAN)
CCDS: CCDS1889.1

-  Methods, Credits, and Use Restrictions
  Click here for details on how this gene model was made and data restrictions if any.