Human Gene TAS2R60 (ENST00000332690.1) from GENCODE V44
  Description: Homo sapiens taste 2 receptor member 60 (TAS2R60), mRNA. (from RefSeq NM_177437)
RefSeq Summary (NM_177437): This gene encodes a member of the bitter taste receptor family which belong to the G protein-coupled receptor superfamily and are predominantly expressed in taste receptor cells of the tongue and palate epithelia. This intronless taste receptor gene encodes a seven-transmembrane receptor protein, functioning as a bitter taste receptor. This gene is clustered together with eight other taste receptor genes on chromosome 7. [provided by RefSeq, Jul 2017].
Gencode Transcript: ENST00000332690.1
Gencode Gene: ENSG00000185899.1
Transcript (Including UTRs)
   Position: hg38 chr7:143,443,453-143,444,409 Size: 957 Total Exon Count: 1 Strand: +
Coding Region
   Position: hg38 chr7:143,443,453-143,444,409 Size: 957 Coding Exon Count: 1 

Page IndexSequence and LinksUniProtKB CommentsPrimersRNA-Seq ExpressionMicroarray Expression
Protein StructureOther SpeciesGO AnnotationsmRNA DescriptionsPathwaysOther Names
Methods
Data last updated at UCSC: 2023-08-18 00:09:47

-  Sequence and Links to Tools and Databases
 
Genomic Sequence (chr7:143,443,453-143,444,409)mRNA (may differ from genome)Protein (318 aa)
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OMIMPubMedReactomeUniProtKBWikipediaBioGrid CRISPR DB

-  Comments and Description Text from UniProtKB
  ID: T2R60_HUMAN
DESCRIPTION: RecName: Full=Taste receptor type 2 member 60; Short=T2R60; AltName: Full=Taste receptor type 2 member 56; Short=T2R56;
FUNCTION: Receptor that may play a role in the perception of bitterness and is gustducin-linked. May play a role in sensing the chemical composition of the gastrointestinal content. The activity of this receptor may stimulate alpha gustducin, mediate PLC-beta-2 activation and lead to the gating of TRPM5 (By similarity).
SUBCELLULAR LOCATION: Membrane; Multi-pass membrane protein.
TISSUE SPECIFICITY: Expressed in subsets of taste receptor cells of the tongue and exclusively in gustducin-positive cells.
MISCELLANEOUS: Most taste cells may be activated by a limited number of bitter compounds; individual taste cells can discriminate among bitter stimuli.
SIMILARITY: Belongs to the G-protein coupled receptor T2R family.

-  Primer design for this transcript
 

Primer3Plus can design qPCR Primers that straddle exon-exon-junctions, which amplify only cDNA, not genomic DNA.
Click here to load the transcript sequence and exon structure into Primer3Plus

Exonprimer can design one pair of Sanger sequencing primers around every exon, located in non-genic sequence.
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To design primers for a non-coding sequence, zoom to a region of interest and select from the drop-down menu: View > In External Tools > Primer3


-  RNA-Seq Expression Data from GTEx (53 Tissues, 570 Donors)
  Highest median expression: 0.05 RPKM in Skin - Sun Exposed (Lower leg)
Total median expression: 0.16 RPKM



View in GTEx track of Genome Browser    View at GTEx portal     View GTEx Body Map

+  Microarray Expression Data
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-  Protein Domain and Structure Information
  InterPro Domains: Graphical view of domain structure
IPR007960 - TAS2_rcpt

Pfam Domains:
PF05296 - Taste receptor protein (TAS2R)

ModBase Predicted Comparative 3D Structure on P59551
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-  Orthologous Genes in Other Species
  Orthologies between human, mouse, and rat are computed by taking the best BLASTP hit, and filtering out non-syntenic hits. For more distant species reciprocal-best BLASTP hits are used. Note that the absence of an ortholog in the table below may reflect incomplete annotations in the other species rather than a true absence of the orthologous gene.
MouseRatZebrafishD. melanogasterC. elegansS. cerevisiae
Genome BrowserGenome BrowserNo orthologNo orthologNo orthologNo ortholog
Gene Details     
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Protein SequenceProtein Sequence    
AlignmentAlignment    

-  Gene Ontology (GO) Annotations with Structured Vocabulary
  Molecular Function:
GO:0004871 signal transducer activity
GO:0004930 G-protein coupled receptor activity
GO:0033038 bitter taste receptor activity

Biological Process:
GO:0007165 signal transduction
GO:0007186 G-protein coupled receptor signaling pathway
GO:0050896 response to stimulus
GO:0050909 sensory perception of taste
GO:0050913 sensory perception of bitter taste
GO:0001580 detection of chemical stimulus involved in sensory perception of bitter taste

Cellular Component:
GO:0005886 plasma membrane
GO:0016020 membrane
GO:0016021 integral component of membrane


-  Descriptions from all associated GenBank mRNAs
  AB529260 - Synthetic construct DNA, clone: pF1KE0836, Homo sapiens TAS2R60 gene for taste receptor, type 2, member 60, without stop codon, in Flexi system.
KJ896044 - Synthetic construct Homo sapiens clone ccsbBroadEn_05438 TAS2R60 gene, encodes complete protein.
KJ905657 - Synthetic construct Homo sapiens clone ccsbBroadEn_15303 TAS2R60-like gene, encodes complete protein.
BC100938 - Homo sapiens taste receptor, type 2, member 60, mRNA (cDNA clone MGC:119154 IMAGE:40004056), complete cds.
BC100939 - Homo sapiens taste receptor, type 2, member 60, mRNA (cDNA clone MGC:119155 IMAGE:40004057), complete cds.
BC100940 - Homo sapiens taste receptor, type 2, member 60, mRNA (cDNA clone MGC:119156 IMAGE:40004058), complete cds.
BC100941 - Homo sapiens cDNA clone IMAGE:40004061, containing frame-shift errors.

-  Biochemical and Signaling Pathways
  KEGG - Kyoto Encyclopedia of Genes and Genomes
hsa04742 - Taste transduction

Reactome (by CSHL, EBI, and GO)

Protein P59551 (Reactome details) participates in the following event(s):

R-HSA-3296233 T2Rs bind bitter-tasting compounds
R-HSA-749454 The Ligand:GPCR:Gi complex dissociates
R-HSA-749456 Liganded Gi-activating GPCRs bind inactive heterotrimeric G-protein Gi
R-HSA-380073 Liganded Gi-activating GPCR acts as a GEF for Gi
R-HSA-420499 Class C/3 (Metabotropic glutamate/pheromone receptors)
R-HSA-418594 G alpha (i) signalling events
R-HSA-500792 GPCR ligand binding
R-HSA-388396 GPCR downstream signalling
R-HSA-372790 Signaling by GPCR
R-HSA-162582 Signal Transduction

-  Other Names for This Gene
  Alternate Gene Symbols: A4D2G8, NM_177437, P59551, Q645W8, Q7RTR7, T2R60_HUMAN, uc011ktg.1, uc011ktg.2
UCSC ID: ENST00000332690.1
RefSeq Accession: NM_177437
Protein: P59551 (aka T2R60_HUMAN or TR60_HUMAN)
CCDS: CCDS5885.1

-  Methods, Credits, and Use Restrictions
  Click here for details on how this gene model was made and data restrictions if any.