Human Gene PTGS2 (ENST00000367468.10) Description and Page Index
Description: Homo sapiens prostaglandin-endoperoxide synthase 2 (PTGS2), mRNA. (from RefSeq NM_000963) RefSeq Summary (NM_000963): Prostaglandin-endoperoxide synthase (PTGS), also known as cyclooxygenase, is the key enzyme in prostaglandin biosynthesis, and acts both as a dioxygenase and as a peroxidase. There are two isozymes of PTGS: a constitutive PTGS1 and an inducible PTGS2, which differ in their regulation of expression and tissue distribution. This gene encodes the inducible isozyme. It is regulated by specific stimulatory events, suggesting that it is responsible for the prostanoid biosynthesis involved in inflammation and mitogenesis. [provided by RefSeq, Feb 2009]. Sequence Note: This RefSeq record was created from transcript and genomic sequence data to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on transcript alignments. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: BC013734.1, SRR1660807.206449.1 [ECO:0000332] ##Evidence-Data-END## ##RefSeq-Attributes-START## MANE Ensembl match :: ENST00000367468.10/ ENSP00000356438.5 RefSeq Select criteria :: based on single protein-coding transcript ##RefSeq-Attributes-END## Gencode Transcript: ENST00000367468.10 Gencode Gene: ENSG00000073756.12 Transcript (Including UTRs) Position: hg38 chr1:186,671,791-186,680,423 Size: 8,633 Total Exon Count: 10 Strand: - Coding Region Position: hg38 chr1:186,674,353-186,680,290 Size: 5,938 Coding Exon Count: 10
ID:PGH2_HUMAN DESCRIPTION: RecName: Full=Prostaglandin G/H synthase 2; EC=18.104.22.168; AltName: Full=Cyclooxygenase-2; Short=COX-2; AltName: Full=PHS II; AltName: Full=Prostaglandin H2 synthase 2; Short=PGH synthase 2; Short=PGHS-2; AltName: Full=Prostaglandin-endoperoxide synthase 2; Flags: Precursor; FUNCTION: Mediates the formation of prostaglandins from arachidonate. May have a role as a major mediator of inflammation and/or a role for prostanoid signaling in activity-dependent plasticity. CATALYTIC ACTIVITY: Arachidonate + AH(2) + 2 O(2) = prostaglandin H(2) + A + H(2)O. COFACTOR: Binds 1 heme B (iron-protoporphyrin IX) group per subunit (By similarity). BIOPHYSICOCHEMICAL PROPERTIES: Kinetic parameters: KM=16.2 uM for arachidonate (in absence of sodium nitroprusside NO donor); KM=17.0 uM for arachidonate (in presence of sodium nitroprusside NO donor); Vmax=81.3 nmol/min/mg enzyme (in absence of sodium nitroprusside NO donor); Vmax=132 nmol/min/mg enzyme (in absence of sodium nitroprusside NO donor); PATHWAY: Lipid metabolism; prostaglandin biosynthesis. SUBUNIT: Homodimer (By similarity). SUBCELLULAR LOCATION: Microsome membrane; Peripheral membrane protein. Endoplasmic reticulum membrane; Peripheral membrane protein. INDUCTION: By cytokines and mitogens. PTM: S-nitrosylation by NOS2 (iNOS) activates enzme activity. S- nitrosylation may take place on different Cys residues in addition to Cys-561. MISCELLANEOUS: This enzyme acts both as a dioxygenase and as a peroxidase. MISCELLANEOUS: This enzyme is the target of nonsteroidal anti- inflammatory drugs such as aspirin. SIMILARITY: Belongs to the prostaglandin G/H synthase family. SIMILARITY: Contains 1 EGF-like domain. WEB RESOURCE: Name=Atlas of Genetics and Cytogenetics in Oncology and Haematology; URL="http://atlasgeneticsoncology.org/Genes/PTGS2ID509ch1q31.html"; WEB RESOURCE: Name=NIEHS-SNPs; URL="http://egp.gs.washington.edu/data/ptgs2/"; WEB RESOURCE: Name=SeattleSNPs; URL="http://pga.gs.washington.edu/data/ptgs2/";
The RNAfold program from the Vienna RNA Package is used to perform the secondary structure predictions and folding calculations. The estimated folding energy is in kcal/mol. The more negative the energy, the more secondary structure the RNA is likely to have.
ModBase Predicted Comparative 3D Structure on P35354
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Orthologous Genes in Other Species
Orthologies between human, mouse, and rat are computed by taking the best BLASTP hit, and filtering out non-syntenic hits. For more distant species reciprocal-best BLASTP hits are used. Note that the absence of an ortholog in the table below may reflect incomplete annotations in the other species rather than a true absence of the orthologous gene.