Human Gene NPFFR2 (ENST00000308744.10)
  Description: Homo sapiens neuropeptide FF receptor 2 (NPFFR2), transcript variant 1, mRNA. (from RefSeq NM_004885)
RefSeq Summary (NM_004885): This gene encodes a member of a subfamily of G-protein-coupled neuropeptide receptors. This protein is activated by the neuropeptides A-18-amide (NPAF) and F-8-amide (NPFF) and may function in pain modulation and regulation of the opioid system. Alternative splicing results in multiple transcript variants. [provided by RefSeq, Jan 2009].
Gencode Transcript: ENST00000308744.10
Gencode Gene: ENSG00000056291.17
Transcript (Including UTRs)
   Position: hg38 chr4:72,031,804-72,148,067 Size: 116,264 Total Exon Count: 4 Strand: +
Coding Region
   Position: hg38 chr4:72,031,902-72,147,812 Size: 115,911 Coding Exon Count: 4 

Page IndexSequence and LinksUniProtKB CommentsMalaCardsCTDRNA-Seq Expression
Microarray ExpressionRNA StructureProtein StructureOther SpeciesGO AnnotationsmRNA Descriptions
PathwaysOther NamesMethods
Data last updated at UCSC: 2021-01-14 15:32:12

-  Sequence and Links to Tools and Databases
Genomic Sequence (chr4:72,031,804-72,148,067)mRNA (may differ from genome)Protein (522 aa)
Gene SorterGenome BrowserOther Species FASTAGene interactionsTable SchemaBioGPS
CGAPEnsemblEntrez GeneExonPrimerGeneCardsHGNC

-  Comments and Description Text from UniProtKB
DESCRIPTION: RecName: Full=Neuropeptide FF receptor 2; AltName: Full=G-protein coupled receptor 74; AltName: Full=G-protein coupled receptor HLWAR77; AltName: Full=Neuropeptide G-protein coupled receptor;
FUNCTION: Receptor for NPAF (A-18-F-amide) and NPFF (F-8-F-amide) neuropeptides, also known as morphine-modulating peptides. Can also be activated by a variety of naturally occurring or synthetic FMRF-amide like ligands. This receptor mediates its action by association with G proteins that activate a phosphatidylinositol- calcium second messenger system.
SUBCELLULAR LOCATION: Cell membrane; Multi-pass membrane protein.
TISSUE SPECIFICITY: Isoform 1 is abundant in placenta. Relatively highly expressed in thymus, testis, and small intestine. Expressed at low levels in several tissues including spleen, prostate, brain, heart, ovary, colon, kidney, lung, liver and pancreas and not expressed in skeletal muscle and leukocytes. Isoform 2 expression is highest in placenta (but at relatively low level compared to isoform 1). Very low level of expression in numerous tissues including adipose tissue and many brain regions. Isoform 3 is expressed in brain and heart and, at lower levels, in kidney, liver, lung and pancreas.
SIMILARITY: Belongs to the G-protein coupled receptor 1 family.
SEQUENCE CAUTION: Sequence=AAK58513.1; Type=Frameshift; Positions=503;

-  MalaCards Disease Associations
  MalaCards Gene Search: NPFFR2
Diseases sorted by gene-association score: hypotrichosis 1 (5)

-  Comparative Toxicogenomics Database (CTD)
  The following chemicals interact with this gene

-  RNA-Seq Expression Data from GTEx (53 Tissues, 570 Donors)
  Highest median expression: 0.31 RPKM in Testis
Total median expression: 2.02 RPKM

View in GTEx track of Genome Browser    View at GTEx portal     View GTEx Body Map

+  Microarray Expression Data
  Press "+" in the title bar above to open this section.

-  mRNA Secondary Structure of 3' and 5' UTRs
RegionFold EnergyBasesEnergy/Base
Display As
5' UTR -23.5098-0.240 Picture PostScript Text
3' UTR -44.24255-0.173 Picture PostScript Text

The RNAfold program from the Vienna RNA Package is used to perform the secondary structure predictions and folding calculations. The estimated folding energy is in kcal/mol. The more negative the energy, the more secondary structure the RNA is likely to have.

-  Protein Domain and Structure Information
  InterPro Domains: Graphical view of domain structure
IPR000276 - 7TM_GPCR_Rhodpsn
IPR017452 - GPCR_Rhodpsn_supfam
IPR005395 - NPFF_rcpt
IPR005397 - NPFF_rcpt_2

Pfam Domains:
PF00001 - 7 transmembrane receptor (rhodopsin family)

ModBase Predicted Comparative 3D Structure on Q9Y5X5
The pictures above may be empty if there is no ModBase structure for the protein. The ModBase structure frequently covers just a fragment of the protein. You may be asked to log onto ModBase the first time you click on the pictures. It is simplest after logging in to just click on the picture again to get to the specific info on that model.

-  Orthologous Genes in Other Species
  Orthologies between human, mouse, and rat are computed by taking the best BLASTP hit, and filtering out non-syntenic hits. For more distant species reciprocal-best BLASTP hits are used. Note that the absence of an ortholog in the table below may reflect incomplete annotations in the other species rather than a true absence of the orthologous gene.
MouseRatZebrafishD. melanogasterC. elegansS. cerevisiae
No orthologGenome BrowserNo orthologGenome BrowserNo orthologNo ortholog
 RGD Ensembl  
 Protein Sequence Protein Sequence  
 Alignment Alignment  

-  Gene Ontology (GO) Annotations with Structured Vocabulary
  Molecular Function:
GO:0004871 signal transducer activity
GO:0004930 G-protein coupled receptor activity
GO:0008188 neuropeptide receptor activity
GO:0031628 opioid receptor binding
GO:0042277 peptide binding

Biological Process:
GO:0007165 signal transduction
GO:0007186 G-protein coupled receptor signaling pathway
GO:0007218 neuropeptide signaling pathway
GO:0009582 detection of abiotic stimulus
GO:0032870 cellular response to hormone stimulus
GO:0043408 regulation of MAPK cascade
GO:0045761 regulation of adenylate cyclase activity
GO:1901652 response to peptide
GO:2000479 regulation of cAMP-dependent protein kinase activity

Cellular Component:
GO:0005886 plasma membrane
GO:0005887 integral component of plasma membrane
GO:0015629 actin cytoskeleton
GO:0016020 membrane
GO:0016021 integral component of membrane

-  Descriptions from all associated GenBank mRNAs
  AF119815 - Homo sapiens G-protein coupled receptor (NPGPR) mRNA, complete cds.
AK308069 - Homo sapiens cDNA, FLJ98017.
AJ311393 - Homo sapiens mRNA for Neuropeptide G-Protein-Coupled Receptor (NPGPR gene), short isoform.
AK290937 - Homo sapiens cDNA FLJ75415 complete cds, highly similar to Homo sapiens G protein-coupled receptor 74 (GPR74), transcript variant 2, mRNA.
AF236083 - Homo sapiens G-protein-coupled receptor 74 (GPR74) mRNA, complete cds.
BC101636 - Homo sapiens neuropeptide FF receptor 2, mRNA (cDNA clone MGC:126685 IMAGE:8069142), complete cds.
BC113713 - Homo sapiens neuropeptide FF receptor 2, mRNA (cDNA clone MGC:142273 IMAGE:8322765), complete cds.
AF257210 - Homo sapiens G-protein coupled receptor HLWAR77 mRNA, complete cds.
JD482133 - Sequence 463157 from Patent EP1572962.
AF268899 - Homo sapiens neuropeptide FF receptor 2 (NPFF2) mRNA, complete cds.
AF330053 - Homo sapiens neuropeptide NPFF receptor mRNA, complete cds.

-  Biochemical and Signaling Pathways
  KEGG - Kyoto Encyclopedia of Genes and Genomes
hsa04080 - Neuroactive ligand-receptor interaction

Reactome (by CSHL, EBI, and GO)

Protein Q9Y5X5 (Reactome details) participates in the following event(s):

R-HSA-389491 Two NPFF receptors can bind neuropeptide FF
R-HSA-749452 The Ligand:GPCR:Gq complex dissociates
R-HSA-749448 Liganded Gq-activating GPCRs bind inactive heterotrimeric Gq
R-HSA-379048 Liganded Gq/11-activating GPCRs act as GEFs for Gq/11
R-HSA-389397 Orexin and neuropeptides FF and QRFP bind to their respective receptors
R-HSA-416476 G alpha (q) signalling events
R-HSA-375276 Peptide ligand-binding receptors
R-HSA-388396 GPCR downstream signalling
R-HSA-373076 Class A/1 (Rhodopsin-like receptors)
R-HSA-372790 Signaling by GPCR
R-HSA-500792 GPCR ligand binding
R-HSA-162582 Signal Transduction

-  Other Names for This Gene
  Alternate Gene Symbols: ENST00000308744.1, ENST00000308744.2, ENST00000308744.3, ENST00000308744.4, ENST00000308744.5, ENST00000308744.6, ENST00000308744.7, ENST00000308744.8, ENST00000308744.9, GPR74, NM_004885, NPFF2, NPFF2_HUMAN, NPGPR, Q96RV1, Q9NR49, Q9Y5X5, uc003hgg.1, uc003hgg.2, uc003hgg.3
UCSC ID: ENST00000308744.10
RefSeq Accession: NM_004885
Protein: Q9Y5X5 (aka NPFF2_HUMAN or NFF2_HUMAN)
CCDS: CCDS3551.1

-  Methods, Credits, and Use Restrictions
  Click here for details on how this gene model was made and data restrictions if any.