Gene interactions and pathways from curated databases and text-mining
Cell 2002, PMID: 12150926

Raptor, a binding partner of target of rapamycin (TOR), mediates TOR action.

Hara, Kenta; Maruki, Yoshiko; Long, Xiaomeng; Yoshino, Ken-ichi; Oshiro, Noriko; Hidayat, Sujuti; Tokunaga, Chiharu; Avruch, Joseph; Yonezawa, Kazuyoshi

mTOR controls cell growth, in part by regulating p70 S6 kinase alpha (p70alpha) and eukaryotic initiation factor 4E binding protein 1 (4EBP1). Raptor is a 150 kDa mTOR binding protein that also binds 4EBP1 and p70alpha. The binding of raptor to mTOR is necessary for the mTOR-catalyzed phosphorylation of 4EBP1 in vitro, and it strongly enhances the mTOR kinase activity toward p70alpha. Rapamycin or amino acid withdrawal increases, whereas insulin strongly inhibits, the recovery of 4EBP1 and raptor on 7-methyl-GTP Sepharose. Partial inhibition of raptor expression by RNA interference (RNAi) reduces mTOR-catalyzed 4EBP1 phosphorylation in vitro. RNAi of C. elegans raptor yields an array of phenotypes that closely resemble those produced by inactivation of Ce-TOR. Thus, raptor is an essential scaffold for the mTOR-catalyzed phosphorylation of 4EBP1 and mediates TOR action in vivo.

Document information provided by NCBI PubMed

Text Mining Data

4EBP1 → raptor: " The binding of raptor to mTOR is necessary for the mTOR catalyzed phosphorylation of 4EBP1 in vitro, and it strongly enhances the mTOR kinase activity toward p70alpha "

4EBP1 → mTOR: " The binding of raptor to mTOR is necessary for the mTOR catalyzed phosphorylation of 4EBP1 in vitro, and it strongly enhances the mTOR kinase activity toward p70alpha "

mTOR → raptor: " The binding of raptor to mTOR is necessary for the mTOR catalyzed phosphorylation of 4EBP1 in vitro, and it strongly enhances the mTOR kinase activity toward p70alpha "

4EBP1 → raptor: " Partial inhibition of raptor expression by RNA interference ( RNAi ) reduces mTOR catalyzed 4EBP1 phosphorylation in vitro "

Manually curated Databases

  • IRef Biogrid Interaction: RPTOR — RPS6KB1 (physical association, affinity chromatography technology)
  • IRef Biogrid Interaction: PDK1 — RPTOR (physical association, affinity chromatography technology)
  • IRef Biogrid Interaction: RPS6KB1 — MTOR (physical association, affinity chromatography technology)
  • IRef Biogrid Interaction: RPTOR — MTOR (physical association, affinity chromatography technology)
  • IRef Biogrid Interaction: EIF4EBP1 — MTOR (direct interaction, pull down)
  • IRef Biogrid Interaction: EIF4EBP1 — MTOR (physical association, affinity chromatography technology)
  • IRef Biogrid Interaction: EIF4EBP1 — RPTOR (direct interaction, pull down)
  • IRef Biogrid Interaction: EIF4EBP1 — RPTOR (physical association, affinity chromatography technology)
  • MIPS CORUM mTOR-signaling complex (mTOR/FRAP1, RAPTOR): mTOR-signaling complex (mTOR/FRAP1, RAPTOR) complex (MTOR-RPTOR)
  • IRef Corum Interaction: RPTOR — MTOR (association, coimmunoprecipitation)
  • IRef Hprd Interaction: RPTOR — RPS6KB1 (in vitro)
  • IRef Hprd Interaction: RPTOR — MTOR (in vitro)
  • IRef Hprd Interaction: RPTOR — MTOR (in vivo)
  • IRef Hprd Interaction: RPTOR — EIF4EBP1 (in vitro)
  • IRef Hprd Interaction: RPTOR — EIF4EBP1 (in vivo)
  • IRef Hprd Interaction: Complex of 28 proteins (in vivo)
  • IRef Innatedb Interaction: EIF4EBP1 — MTOR (unknown, -)
  • IRef Intact Interaction: Complex of RPTOR (association, anti tag coimmunoprecipitation)
  • IRef Intact Interaction: RPTOR — MTOR (physical association, anti bait coimmunoprecipitation)
  • IRef Intact Interaction: EIF4EBP1 — MTOR (phosphorylation reaction, protein kinase assay)
  • IRef Intact Interaction: RPTOR — EIF4EBP1 (physical association, anti tag coimmunoprecipitation)
  • IRef Intact Interaction: RPTOR — EIF4EBP1 (physical association, pull down)
  • IRef Intact Interaction: Complex of RPTOR-EIF4EBP1-RPTOR-EIF4EBP1 (association, anti tag coimmunoprecipitation)
  • Reactome Reaction: MTOR → EIF4E (reaction)
  • Reactome Reaction: RHEB → EIF4E (reaction)
  • Reactome Reaction: MTOR → EIF4EBP1 (reaction)
  • Reactome Reaction: EIF4E → RPTOR (reaction)
  • Reactome Reaction: EIF4EBP1 → RPTOR (reaction)
  • Reactome Reaction: EIF4E → EIF4EBP1 (direct_complex)
  • Reactome Reaction: RHEB → EIF4EBP1 (reaction)
  • Reactome Reaction: EIF4EBP1 → MLST8 (reaction)
  • Reactome Reaction: EIF4E → MLST8 (reaction)
In total, 13 gene pairs are associated to this article in curated databases