Gene interactions and pathways from curated databases and text-mining
Curr Eye Res 2013, PMID: 23281820

Rapamycin enhances TNF-α-induced secretion of IL-6 and IL-8 through suppressing PDCD4 degradation in orbital fibroblasts.

Lee, Won-Mo; Paik, Ji-Sun; Cho, Won-Kyung; Oh, Eun-Hye; Lee, Seong-Beom; Yang, Suk-Woo

OBJECTIVE

To investigate the effects of rapamycin on the TNF-α-induced secretion of interleukin-6 (IL-6) and IL-8 in orbital fibroblasts and its possible mechanism.

METHODS

Orbital fibroblasts were obtained from patients with thyroid-associated ophthalmopathy. IL-6 and IL-8 levels were measured by enzyme-linked immunosorbent assays. The down-regulation of PDCD4 was performed by PDCD4 siRNA transfection.

RESULTS

Rapamycin significantly enhanced TNF-α-induced IL-6 and IL-8 secretion from orbital fibroblasts. Down-regulation of PDCD4 by PDCD4 siRNA transfection reduced TNF-α-induced IL-6 and IL-8 secretion from orbital fibroblasts. In addition, TNF-α was found to promote the mTOR-dependent proteasome-mediated degradation of PDCD4. Rapamycin increased PDCD4 expression via the inhibition of TNF-α-induced PDCD4 degradation in orbital fibroblasts.

CONCLUSIONS

Rapamycin enhances the TNF-α-induced secretion of IL-6 and IL-8 by suppressing PDCD4 degradation in orbital fibroblasts.

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Text Mining Data

interleukin-6 (IL-6) → TNF-a: " Purpose : To investigate the effects of rapamycin on the TNF-a induced secretion of interleukin-6 (IL-6) and IL-8 in orbital fibroblasts and its possible mechanism "

IL-8 → TNF-a: " Purpose : To investigate the effects of rapamycin on the TNF-a induced secretion of interleukin-6 (IL-6) and IL-8 in orbital fibroblasts and its possible mechanism "

IL-6 → TNF-a: " Results : Rapamycin significantly enhanced TNF-a induced IL-6 and IL-8 secretion from orbital fibroblasts "

IL-8 → TNF-a: " Results : Rapamycin significantly enhanced TNF-a induced IL-6 and IL-8 secretion from orbital fibroblasts "

IL-6 → TNF-a: " Down-regulation of PDCD4 by PDCD4 siRNA transfection reduced TNF-a induced IL-6 and IL-8 secretion from orbital fibroblasts "

IL-6 → PDCD4: " Down-regulation of PDCD4 by PDCD4 siRNA transfection reduced TNF-a induced IL-6 and IL-8 secretion from orbital fibroblasts "

IL-8 → TNF-a: " Down-regulation of PDCD4 by PDCD4 siRNA transfection reduced TNF-a induced IL-6 and IL-8 secretion from orbital fibroblasts "

IL-8 → PDCD4: " Down-regulation of PDCD4 by PDCD4 siRNA transfection reduced TNF-a induced IL-6 and IL-8 secretion from orbital fibroblasts "

PDCD4 → mTOR: " In addition, TNF-a was found to promote the mTOR dependent proteasome mediated degradation of PDCD4 "

PDCD4 → TNF-a: " Rapamycin increased PDCD4 expression via the inhibition of TNF-a induced PDCD4 degradation in orbital fibroblasts "

IL-6 → TNF-a: " Conclusion : Rapamycin enhances the TNF-a induced secretion of IL-6 and IL-8 by suppressing PDCD4 degradation in orbital fibroblasts "

IL-8 → TNF-a: " Conclusion : Rapamycin enhances the TNF-a induced secretion of IL-6 and IL-8 by suppressing PDCD4 degradation in orbital fibroblasts "

Manually curated Databases

No curated data.