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BRAF — RAD50
Text-mined interactions from Literome
Okada et al., Mol Cell Biol 1999
:
B-Raf carrying this mutation was not
activated by
Rap1A but retained its response to Ras
Carey et al., J Biol Chem 2003
:
In contrast to Raf-1,
B-Raf is
activated by
Rap1
Dillon et al., J Biol Chem 2003
:
Recent studies suggest that
Rap1 can
activate the MAP kinase kinase kinase
B-Raf that is either endogenously or ectopically expressed
Calipel et al., J Biol Chem 2006
(Cell Transformation, Neoplastic...) :
This ruled out a
role for
Rap1 in the PKA mediated
B-Raf/ERK activation in ( WT ) B-Raf cells
Wang et al., Mol Cell Biol 2006
:
Rap1 activation by Epac-CAAX, but not wild-type Epac,
triggers its association with
B-Raf
Ohtsuka et al., J Biol Chem 1996
:
Here we have used this assay system and examined the
effect of
Rap1 on the
B-Raf activity to phosphorylate recombinant MAP kinase kinase ( MEK ) ... Recombinant
Rap1B stimulated the activity of
B-Raf , which was partially purified from bovine brain and immunoprecipitated by an anti-B-Raf antibody ...
Rap1B enhanced the Ki-Ras stimulated
B-Raf activity in an additive manner
Kuroda et al., J Biol Chem 1996
:
Different effects of various phospholipids on Ki-Ras-, Ha-Ras-, and
Rap1B induced
B-Raf activation ... Moreover, we found that
Rap1B as well as Ki-Ras and Ha-Ras
stimulate the
B-Raf activity ... Since B-Raf contains a cysteine-rich domain originally found in protein kinase C as a domain responsible for interaction with phosphatidylserine ( PS ) and diacylglycerol or 12-O-tetradecanoylphorbol-13-acetate, we have examined here the effect of these compounds on the Ki-Ras-, Ha-Ras-, and
Rap1B induced
activation of bovine brain
B-Raf ... However, none of the above phospholipids affected the Ha-Ras stimulated B-Raf activity, whereas PI, PS, phosphatidylethanolamine, and phosphatidic acid inhibited the
Rap1B stimulated
B-Raf activity ... Phosphatidylcholine or PI-4-monophosphate did not show any effect on the
Rap1B stimulated
B-Raf activity ... Synthetic PS with two unsaturated fatty acids, such as 1,2-dioleoyl-PS or 1,2-dilinoleoyl-PS, showed the same effect toward the Ki-Ras- and
Rap1B stimulated
B-Raf activities, but synthetic PS with two saturated fatty acids, such as 1, 2-distearoyl-PS, was inactive ... 12-O-Tetradecanoylphorbol-13-acetate did not affect the stimulatory or inhibitory effect of PS on the Ki-Ras- and
Rap1B stimulated
B-Raf activities, respectively ... These results indicate that various phospholipids differently affect Ki-Ras-, Ha-Ras, and
Rap1B induced
B-Raf activation
Vossler et al., Cell 1997
:
These cell type-specific actions of cAMP require the expression of the serine/threonine kinase
B-Raf and
activation of the small G protein
Rap1 ...
Rap1 , activated by mutation or by the cAMP dependent protein kinase PKA, is a selective
activator of
B-Raf and an inhibitor of Raf-1