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NGF — RHOA
Pathways - manually collected, often from reviews:
-
NCI Pathway Database Neurotrophic factor-mediated Trk receptor signaling:
Rho family GTPase (RAC1/RHOA/CDC42)
→
NGF (dimer)/TRKA/GRIT complex (NTRK1-NGF-ARHGAP32)
(modification, collaborate)
Nakamura et al., Mol Cell Biol 2002
Evidence: assay, other species
-
NCI Pathway Database p75(NTR)-mediated signaling:
NGF (dimer)/p75(NTR)/PKA complex (NGF-NGFR-PRKACB)
→
RhoA-B-C/GDP complex (RHOC_RHOA_RHOB)
(modification, activates)
Yamashita et al., Neuron 1999, Higuchi et al., EMBO J 2003
Evidence: mutant phenotype, assay
-
Reactome Reaction:
NGF
→
RHOA
(indirect_complex)
Gehler et al., J Neurosci 2004*
-
Reactome Reaction:
NGF
→
RHOA
(reaction)
Gehler et al., J Neurosci 2004*
Text-mined interactions from Literome
Yamaguchi et al., J Biol Chem 2001
:
NGF induced a rapid activation of Rac1 and suppression of
RhoA activity
Liu et al., Mol Cell Neurosci 2002
:
Inhibition of the small GTPase
RhoA by the dominant negative mutant RhoA ( T19N )
restored NGF responsiveness of axon growth on L1 to Bax ( -/- ) neurons
Nusser et al., J Biol Chem 2002
:
The inhibitory pathway from TrkA to RhoA involves phosphatidylinositol-3-kinase (PI3K), because the inhibitors LY294002 or wortmannin prevented
NGF induced
RhoA translocation and increased RhoA association with ROK
Nusser et al., Cell Signal 2006
:
However, when monitoring
NGF induced binding of
GTP-RhoA to multiple targets, we noted differential interactions with its effectors ... In the context of PC12 cell differentiation,
NGF induced phosphorylation of
RhoA on serine ( 188 ) prevents it from interacting with ROK, which would otherwise block neurite outgrowth
Noga et al., Clin Exp Allergy 2007
(Hypersensitivity) :
NGF led to decreased
RhoA and increased Rac activation, while BDNF affected RhoA and Rac activity in a reciprocal fashion
Jeon et al., Exp Mol Med 2010
:
p190RhoGAP and Rap dependent RhoGAP ( ARAP3 ) inactivate
RhoA in
response to
nerve growth factor leading to neurite outgrowth from PC12 cells ...
NGF suppressed
GTP-RhoA levels after 12 h in PC12 cells and was consistently required for a long time to induce neurite outgrowth ... Constitutively active ( CA )
-RhoA suppressed neurite outgrowth from PC12 cells in
response to
NGF , whereas dominant negative ( DN ) -RhoA stimulated it, suggesting that RhoA inactivation is essential for neurite outgrowth ... DN-p190RhoGAP abrogated neurite outgrowth, whereas wild-type ( WT ) -p190RhoGAP and WT-Src synergistically stimulated it along with accelerating RhoA inactivation, suggesting that p190RhoGAP, which can be activated by Src, is a major component in inhibiting
RhoA in
response to
NGF in PC12 cells