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EPHB2 — PEA15
Text-mined interactions from Literome
Ramos et al., Mol Biol Cell 2000
:
PEA-15 expression in Chinese hamster ovary cells
resulted in an increased mitogen activated protein kinase kinase and
ERK activity
Hill et al., EMBO J 2002
:
In particular,
PEA-15 regulates the actions of the ERK MAP kinase cascade by binding to
ERK and altering its subcellular localization
Renault et al., Biochem Pharmacol 2003
:
In particular,
PEA-15 diverts astrocytes from TNFalpha triggered apoptosis and
regulates the actions of the ERK MAP kinase cascade by binding to
ERK and altering its subcellular localization
Whitehurst et al., J Biol Chem 2004
(MAP Kinase Signaling System) :
Consistent with its recognition of the MAP kinase insert,
PEA-15 blocked activation of
ERK2 by MEK1, which also requires the MAP kinase insert to interact productively with ERK2 ... To determine how PEA-15 influences the localization of ERK2, we used a permeabilized cell system to examine the
effect of
PEA-15 on the localization of
ERK2 and mutants that have lost the ability to bind PEA-15
Callaway et al., Biochim Biophys Acta 2005
:
Quantifying ERK2-protein interactions by fluorescence anisotropy :
PEA-15 inhibits
ERK2 by blocking the binding of DEJL domains ... The
regulation of
ERK2 by
PEA-15 is appraised in the light of a simple equilibrium binding model for reversible ERK2 nucleoplasmic-cytoplasmic shuttling, which elaborates on the theory of Burack and Shaw ( J. Biol. Chem. 280, 3832-3837 ; 2005 )
Glading et al., Cancer Res 2007
(Breast Neoplasms...) :
The effect of
PEA-15 on tumor invasion is
mediated by its interaction with
ERK1/2 as shown by the following : ( a ) PEA-15 mutants that fail to bind ERK1/2 did not inhibit invasion ; ( b ) overexpression of ERK1 or activated MAP/ERK kinase (MEK) reversed the inhibitory effect of PEA-15 ; ( c ) when an inhibitor of ERK1/2 activation reduced invasion, PEA-15 expression did not significantly reduce invasion further
Callaway et al., Biochemistry 2007
:
Neither the phosphorylation of
PEA-15 ( phospho Ser-104 and/or phospho Ser-116 ) nor the phosphorylation of ERK1/2 ( by MKK1 ) significantly
affects the stability of the
ERK/PEA-15 interaction, and therefore it does not directly regulate the release of ERK2 to the nucleus
Eckert et al., Oncogene 2008
(Brain Neoplasms...) :
PEA-15 regulates the level of phosphorylated extracellular regulated kinase (ERK)1/2 in glioblastoma cells and the PEA-15 dependent protection from glucose deprivation induced cell death
requires ERK1/2 signaling ...
PEA-15 transcriptionally
upregulates the Glucose Transporter 3, which is abrogated by the inhibition of
ERK1/2 phosphorylation
Roth et al., Verh Dtsch Ges Pathol 2007
(Astrocytoma...) :
PEA-15 regulates the level of phosphorylated
ERK1/2 in glioblastoma cells and the PEA-15 dependent protection from glucose deprivation induced cell death requires ERK1/2 signaling
Haling et al., Mol Biol Cell 2010
(MAP Kinase Signaling System) :
This is the dominant mechanism by which
PEA-15 activates
ERK1/2 because genetic deletion of FRS2alpha blocked the capacity of PEA-15 to activate the MAP kinase pathway
Botta et al., Hum Gene Ther 2010
(Adenoviridae Infections...) :
Both ERK2 overexpression and genetic silencing of
PED/PEA-15 by antisense oligonucleotides
increased ERK nuclear accumulation and reduced CAR expression and adenoviral entry
Böck et al., J Biol Chem 2010
(Brain Neoplasms...) :
The mechanism by which
PEA-15 inhibits caspase activation and
increases ERK ( extracellular regulated kinase ) activity is well characterized
Buonomo et al., J Cell Physiol 2012
(Diabetes Mellitus, Type 2...) :
Thus,
PED/PEA-15 may affect fibroblast motility by a mechanism, at least in part,
mediated by
ERK1/2
Sulzmaier et al., Oncogene 2012
(Cell Transformation, Neoplastic...) :
Our findings reveal a novel mechanism by which
PEA-15 positively
regulates Ras/ERK signaling and increases the proliferation of H-Ras transformed epithelial cells through enhanced PLD1 expression and activation ... These data reveal that
PEA-15 not only
suppresses ERK signaling and tumorigenesis but also alternatively enhances tumorigenesis in the context of active Ras
Gawecka et al., Int J Cancer 2012
(Chromosome Aberrations...) :
Phosphoprotein enriched in astrocytes 15 ( PEA15 ) binds
ERK and RSK2 and high
PEA15 levels can
impair ERK- and RSK2 dependent transcription
Funke et al., Cancer Lett 2013
(Colorectal Neoplasms...) :
These effects were associated with a
PEA-15 dependent down-regulation of integrin avß5 as well as with elevated levels of the phosphorylated MAP kinase
ERK1/2
Mace et al., Nature communications 2013
:
The scaffolding protein
PEA-15 is a death effector domain protein that directly interacts with ERK1/2 and
affects ERK1/2 subcellular localization and phosphorylation