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INS — VEGFA

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Lu et al., Invest Ophthalmol Vis Sci 1999 : Insulin induced vascular endothelial growth factor expression in retina ... The current study was conducted to characterize the effect of insulin on retinal VEGF gene expression in vitro and in vivo ... The effect of insulin on VEGF expression in vivo was examined by in situ hybridization studies of rat retinal VEGF transcripts ... To examine the mechanisms by which insulin regulates VEGF expression, human retinal pigment epithelial (RPE) cells were exposed to insulin, and VEGF mRNA levels were quantified with RNase protection assays ( RPAs ) ... Insulin increased VEGF mRNA levels in the ganglion, inner nuclear, and RPE cell layers ... In vitro, insulin increased VEGF mRNA levels in human RPE cells and enhanced VEGF promoter activity without affecting transcript stability ... Insulin increases VEGF mRNA and secreted protein levels in RPE cells through enhanced transcription of the VEGF gene
Miele et al., J Biol Chem 2000 : Insulin and insulin-like growth factor-I induce vascular endothelial growth factor mRNA expression via different signaling pathways ... Blocking mitogen activated protein kinase activation with the MEK inhibitor PD98059 abolished the effect of IGF-I on VEGF mRNA expression in NIH-IGFR cells but had no effect on insulin induced VEGF mRNA expression in NIH-IR cells ... We conclude that VEGF induction by insulin and IGF-I occurs via different signaling pathways, the former involving phosphatidylinositol 3-kinase/protein kinase B and the latter involving MEK/mitogen activated protein kinase
Tulandi et al., Fertil Steril 2000 (Infertility, Female...) : Effects of laparoscopic ovarian drilling on serum vascular endothelial growth factor and on insulin responses to the oral glucose tolerance test in women with polycystic ovary syndrome ... To evaluate the serum vascular endothelial growth factor concentrations and insulin responses to the oral glucose tolerance test before and after laparoscopic ovarian drilling in women with PCOS ... VEGF levels and insulin responses to OGTT before and after ovarian drilling
Chou et al., Circulation 2002 (Diabetes Mellitus...) : Insulin increased VEGF mRNA expression in cultured rat neonatal cardiac myocytes
Mick et al., Endocrinology 2002 : Insulin stimulated VEGF release was mediated in part via PI3K, but not PKC
Poulaki et al., J Clin Invest 2002 (Diabetes Mellitus...) : Insulin induced VEGF expression requires p38 MAPK and PI 3-kinase, whereas hyperglycemia induced VEGF expression is HIF-1alpha independent and requires PKC and p42/p44 MAPK
Agrawal et al., Fertil Steril 2002 (Polycystic Ovary Syndrome) : Insulin alone did not increase release of VEGF , but addition of insulin increased hCG stimulated release of VEGF
Jiang et al., J Biol Chem 2003 : The effects of insulin on vascular endothelial growth factor ( VEGF ) expression in cultured vascular cells and in angiogenesis were characterized ... Insulin increased VEGF mRNA levels in mouse aortic smooth muscle cells from 10 ( -9 ) to 10 ( -7 ) m with an initial peak of 3.7-fold increases at 1 h and a second peak of 2.8-fold after 12 h ... In contrast, the chronic effect of insulin on VEGF expression was partially inhibited by both LY294002 or PD98059 as well as by the overexpression of dominant negatives of PI 3-kinase or Ras ... The importance of PI 3-kinase-Akt pathway on VEGF expression was confirmed in mouse aortic smooth muscle cells isolated from insulin receptor substrate -1 knockout ( IRS-1-/- ) mice that showed parallel reductions of 46-49 % in insulin stimulated VEGF expression and PI 3-kinase-Akt activation ... Thus, unlike other cells, insulin can regulate VEGF expression by both IRS-1/PI 3-kinase-Akt cascade and Ras-MAPK pathways in aortic smooth muscle cells
Wang et al., Horm Metab Res 2003 (Body Weight) : White adipocytes are capable of producing vascular endothelial growth factor ( VEGF ) in response to insulin and catecholamines
Sigrist et al., Cell Transplant 2003 (Diabetes Mellitus, Experimental...) : Insulin secretion of the islets was higher in the presence of VEGF compared with islets alone at all steps of the study
Neid et al., J Biol Chem 2004 (Adenocarcinoma...) : Previously we have shown that in AsPC-1 pancreatic adenocarcinoma cells, insulin-like growth factor receptor ( IGF-IR ) regulates VPF/VEGF expression
Song et al., Zhonghua Yan Ke Za Zhi 2004 : VEGF expression was enhanced obviously by the insulin , which regulated the expression of VEGF at the transcription level
Unsoeld et al., Molecular vision 2004 (Disease Models, Animal...) : VEGF signaling is enhanced by insulin-like growth factor-1 (IGF-1)
Rowe et al., J Am Coll Nutr 2004 (Cardiovascular Diseases...) : Insulin is necessary for VEGF expression
Doronzo et al., Eur J Clin Invest 2004 (Insulin Resistance) : Insulin activates vascular endothelial growth factor in vascular smooth muscle cells : influence of nitric oxide and of insulin resistance ... We found that in VSMCs from humans and from insulin-sensitive Zucker fa/+rats, insulin increases VEGF protein expression and secretion, with mechanisms blunted by wortmannin and LY294002 ( PI3-K inhibitors ), PD98059 ( MAPK inhibitor ), L-NMMA ( NOS inhibitor ) and Rp-8pCT-cGMPs ( PKG inhibitor ) ... The insulin effects on VEGF are impaired in VSMCs from Zucker fa/fa rats, which also present a reduced insulin ability to increase NO ... In VSMCs from humans and insulin-sensitive Zucker fa/+rats : ( i ) insulin increases VEGF protein expression and secretion via both PI3-K and MAPK; (ii) the insulin effects on VEGF are mediated by nitric oxide ... The insulin action on both nitric oxide and VEGF is impaired in VSMCs from Zucker fa/fa rats, an animal model of metabolic and vascular insulin-resistance
Fain et al., Metabolism 2005 : Insulin enhances vascular endothelial growth factor , interleukin-8, and plasminogen activator inhibitor 1 but not interleukin-6 release by human adipocytes ... Dexamethasone inhibited VEGF formation by both adipose tissue explants and isolated adipocytes, whereas insulin stimulated VEGF release only in isolated adipocytes ... In contrast, insulin stimulated VEGF release by human adipocytes, and this stimulation was enhanced in the presence of isoproterenol ... Insulin stimulated VEGF formation as well as that of PAI-1 by human adipocytes, but not by explants under conditions where it had little effect on that of IL-6 ... The ability of insulin to stimulate VEGF formation by adipocytes suggests that the elevated circulating levels of insulin in obesity promote angiogenesis in adipose tissue as well as the enhanced accumulation of fat in human adipocytes
Yang et al., Biochem Biophys Res Commun 2005 (Diabetes Mellitus, Experimental...) : In 3T3-L1 adipocytes, GI262570 did not affect insulin stimulated VEGF secretion
He et al., Arterioscler Thromb Vasc Biol 2006 (Insulin Resistance...) : This was associated with a parallel 40 % inhibition of insulin stimulated activation of both Akt and VEGF expression in the myocardium and cardiomyocytes ... Insulin regulates VEGF gene expression and vascularization in the myocardium specifically via insulin receptors and the activation of PI3K/Akt pathway
Naruse et al., Diabetes 2006 (Atherosclerosis...) : Thus, activation of PKCbeta in endothelial cells and vascular tissue inhibits Akt activation by insulin and VEGF , inhibits Akt dependent eNOS regulation by insulin , and causes endothelial dysfunction in obesity associated insulin resistance
Doronzo et al., Diabetologia 2006 (Insulin Resistance) : As it is not known whether the effects of insulin on VEGF involve activation of hypoxia-inducible factor-1 (HIF-1), we aimed to evaluate : ( 1 ) whether insulin modulates HIF-1alpha protein synthesis and activity ; ( 2 ) the insulin signalling pathways involved ; and ( 3 ) the role of insulin resistance
Treins et al., Biochem Biophys Res Commun 2006 : These results show that AICAR and insulin/IGF-1 regulate VEGF expression through different mechanisms
Zhou et al., Carcinogenesis 2007 (Neovascularization, Pathologic...) : Reactive oxygen species regulate insulin induced VEGF and HIF-1alpha expression through the activation of p70S6K1 in human prostate cancer cells ... H2O2 production is also required for insulin induced VEGF and HIF-1alpha expression in the cells ... These results suggest that insulin induced HIF-1alpha and VEGF expression through H2O2 production and p70S6K1 activation in prostate cancer cells
Carnesecchi et al., Exp Cell Res 2006 : Insulin induced vascular endothelial growth factor expression is mediated by the NADPH oxidase NOX3 ... The aim of this study was to investigate the role of ROS producing NADPH oxidase enzymes ( NOX-es ) in insulin regulated VEGF gene activation ... To achieve this goal we chose HepG2 cells as our model system as these cells express the NADPH oxidase isoform NOX3 and respond to insulin stimulation with enhanced ROS production and mRNA transcription and production of VEGF
Miyamoto et al., Diabetologia 2007 (Diabetic Retinopathy...) : Using RT-PCR, we evaluated the effect of hypoxic conditions or insulin on transepithelial resistance and on PLGF-1 and VEGF receptors
Wang et al., Am J Pathol 2007 (Pancreatic Neoplasms) : Under hypoxic conditions, insulin stimulated glycolysis, cell proliferation, and the secretion of vascular endothelial growth factor in regular MiaPaCa2 cells but not in a MiaPaCa2 variant ( si-MiaPaCa2 ) that expressed specific short interfering RNA for HIF-1alpha and therefore lacked HIF-1alpha protein
Kuo et al., Chang Gung Med J 2006 (Body Weight...) : Effect of insulin on the expression of intraocular vascular endothelial growth factor in diabetic rats ... To investigate the long-term effect of insulin on vascular endothelial growth factor ( VEGF ) of streptozotocin (STZ) induced diabetic rats
Stanek et al., J Clin Endocrinol Metab 2007 (Polycystic Ovary Syndrome) : However, insulin or IGF increased VEGF-A levels within 1 d and appeared to synergize with LH at 3 d. VEGF-A production by non-PCOS LGCs was more sensitive to IGF exposure, whereas PCOS cells were more sensitive to insulin ... Insulin and IGFs promote VEGF-A production in LGCs, but the response patterns are different when cells from PCOS and non-PCOS women are compared
Yoo et al., Invest Ophthalmol Vis Sci 2007 (Disease Models, Animal...) : The induction of VEGF by insulin , as observed in certain conditions, may be a plausible mechanism for this phenomenon
Goren et al., J Invest Dermatol 2009 (Diabetes Mellitus) : Our data suggest that the endocrine hormone insulin contributes to VEGF release in skin wounds through an Akt1 mediated posttranscriptional mechanism in keratinocytes
Jabs et al., Exp Clin Endocrinol Diabetes 2008 : Here, we investigated whether VEGF-A was required for physiological glucose stimulated insulin secretion and insulin content ... We conclude that VEGF-A is required in pancreatic islets for normal glucose stimulated insulin secretion and physiological insulin content
Wu et al., Zhonghua Yan Ke Za Zhi 2008 : [ Effect of insulin on VEGF expression in bovine retinal microvascular endothelial cells exposed to normal or high glucose ] ... To investigate the effect of insulin or ( and ) high glucose on the vascular endothelial growth factor ( VEGF ) expression in bovine retinal microvascular endothelial cells ( BREC ) ... High glucose attenuated insulin induced VEGF expression
Wu et al., Eye (Lond) 2010 (Diabetic Retinopathy) : High glucose attenuates insulin induced VEGF expression in bovine retinal microvascular endothelial cells ... To investigate the effect of high glucose on insulin induced vascular endothelial growth factor ( VEGF ) expression in bovine retinal microvascular endothelial cells ( BRECs ) and to probe into related mechanisms ... Pretreatment of cells with PI3-K inhibitor significantly ( P < 0.05, one-way ANOVA ) suppressed the insulin induced VEGF expression ; neither pretreatment with the PKC inhibitor nor with the P42/p44 MAPK inhibitor showed an effect on the expression of VEGF at the mRNA or protein level ( P > 0.05, one-way ANOVA ) ... Both insulin and high glucose can markedly increase VEGF expression in BRECs at the mRNA and protein level ... We propose that insulin may upregulate VEGF expression through the PI3-K signalling pathway in BRECs, and high glucose may attenuate insulin induced VEGF expression by impairing PI3-K signalling pathways
Villaume et al., Neuroendocrinology 2010 (Neuroendocrine Tumors) : Octreotide and rapamycin induced a significant decrease in VEGF production by all three cell lines ; LY294002 significantly inhibited VEGF production by STC-1 and INS-r3 only
Jiang et al., Can J Physiol Pharmacol 2010 (Diabetes Mellitus, Experimental) : Since insulin/insulin-like growth factor 1 receptor ( IGF-1R ) can activate vascular endothelial growth factor to promote vascular growth, reduced IGF-1R signaling in the type I diabetic heart could be detrimental, leading to reduced, collateral blood vessel growth
Ikeda et al., Mol Cell Biol 2011 (Disease Models, Animal...) : In cultured lung epithelial cells, insulin reduced VEGF expression and transcriptional activity of HIF-2 on VEGF promoter in an mTOR dependent manner
Wu et al., Graefes Arch Clin Exp Ophthalmol 2011 (Diabetic Retinopathy) : Different effects of low- and high-dose insulin on ROS production and VEGF expression in bovine retinal microvascular endothelial cells in the presence of high glucose ... The purpose of the present study was to investigate the effect of insulin on the mitochondrial membrane potential ( ??m ), reactive oxygen species ( ROS ) production, UCP-2 and VEGF expression in bovine retinal microvascular endothelial cells ( BRECs ) in the presence of normal or high glucose and the related mechanisms ... Insulin increased ??m, ROS production, and expression of UCP-2 and VEGF in BRECs at normal glucose ( 5 mM ) in a dose dependent manner ... High-dose insulin induced ROS production and VEGF expression in BRECs in the presence of high glucose might be one of the reasons for the transient worsening of diabetic retinopathy during intensive insulin treatment
Miuma et al., J Cell Biochem 2012 (Carcinoma, Hepatocellular...) : All three BCAA components were required for acceleration of insulin induced VEGF mRNA degradation
Meng et al., PloS one 2012 : NADPH oxidase 4 mediates insulin stimulated HIF-1a and VEGF expression, and angiogenesis in vitro ... Reactive oxygen species ( ROS ) have been shown to be involved in HIF-1a and VEGF expression induced by insulin , but the role of specific ROS sources has not been fully elucidated ... In this study we examined the role of NADPH oxidase subunit 4 (Nox4) in insulin stimulated HIF-1a and VEGF expression, and angiogenic responses in human microvascular endothelial cells ( HMVECs ) ... Inhibition of Nox4 impaired insulin stimulated VEGF expression, cell migration, cell proliferation, and tube formation in HMVECs ... Our data indicate that Nox4 derived ROS are essential for HIF-1a dependent VEGF expression, and angiogenesis in vitro induced by insulin
Muhidin et al., J Obes 2012 (Colorectal Neoplasms...) : Insulin binds to IGF-1 receptors and plays an important role in cell proliferation, apoptosis, and increased production of vascular endothelial growth factor , an angiogenic factor that supports cancer growth
Xiao et al., J Biol Chem 2013 (Hypoglycemia) : VEGF-A expression in beta cells is critical for pancreatic development, formation of islet-specific vasculature, and Insulin secretion
Hale et al., American journal of physiology. Renal physiology 2013 (Insulin Resistance) : Insulin directly stimulates VEGF-A production in the glomerular podocyte ... Using in vitro and in vivo approaches, in the present study, we now show that insulin regulates VEGF-A in the podocyte in both mice and humans via the insulin receptor (IR) ... Insulin directly increased VEGF-A mRNA levels and protein production in conditionally immortalized wild-type human and murine podocytes
Oberg-Welsh et al., Mol Cell Endocrinol 1997 : Glucose stimulated acute insulin release was not affected by VEGF
Zelzer et al., EMBO J 1998 : Furthermore, basal and insulin induced expression of Glut1, Glut3, aldolase A, phosphoglycerate kinase and VEGF was reduced in cells having a defective ARNT ... Similarly, the insulin induced activation of HRE reporter vectors and VEGF was impaired in these cells and was rescued by re-introduction of ARNT
Akagi et al., Cancer Res 1998 (Colonic Neoplasms) : We investigated the role of insulin-like growth factor (IGF)-I and IGF binding proteins ( IGFBPs ) in the regulation of vascular endothelial growth factor ( VEGF ) expression in colon cancer cells and the mechanism by which this regulation occurs