Human Gene NAA15 (uc003ihu.1)
  Description: Homo sapiens N(alpha)-acetyltransferase 15, NatA auxiliary subunit (NAA15), mRNA.
RefSeq Summary (NM_057175): N-alpha-acetylation is among the most common post-translational protein modifications in eukaryotic cells. This process involves the transfer of an acetyl group from acetyl-coenzyme A to the alpha-amino group on a nascent polypeptide and is essential for normal cell function. This gene encodes the auxillary subunit of the N-terminal acetyltransferase A (NatA) complex. [provided by RefSeq, Jan 2017]. Sequence Note: This RefSeq record was created from transcript and genomic sequence data to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on transcript alignments.
Transcript (Including UTRs)
   Position: hg19 chr4:140,222,676-140,311,935 Size: 89,260 Total Exon Count: 20 Strand: +
Coding Region
   Position: hg19 chr4:140,222,932-140,309,238 Size: 86,307 Coding Exon Count: 20 

Page IndexSequence and LinksUniProtKB CommentsPrimersMalaCardsCTD
Gene AllelesRNA-Seq ExpressionMicroarray ExpressionRNA StructureProtein StructureOther Species
GO AnnotationsmRNA DescriptionsOther NamesModel InformationMethods
Data last updated at UCSC: 2013-06-14

-  Sequence and Links to Tools and Databases
 
Genomic Sequence (chr4:140,222,676-140,311,935)mRNA (may differ from genome)Protein (866 aa)
Gene SorterGenome BrowserOther Species FASTAVisiGeneGene interactionsTable Schema
AlphaFoldBioGPSEnsemblEntrez GeneExonPrimerGeneCards
GeneNetworkH-INVHGNCHPRDLynxMalacards
MGIneXtProtOMIMPubMedTreefamUniProtKB
WikipediaBioGrid CRISPR DB

-  Comments and Description Text from UniProtKB
  ID: NAA15_HUMAN
DESCRIPTION: RecName: Full=N-alpha-acetyltransferase 15, NatA auxiliary subunit; AltName: Full=Gastric cancer antigen Ga19; AltName: Full=N-terminal acetyltransferase; AltName: Full=NMDA receptor-regulated protein 1; AltName: Full=Protein tubedown-1; AltName: Full=Tbdn100;
FUNCTION: The NAA10-NAA15 complex displays alpha (N-terminal) acetyltransferase activity that may be important for vascular, hematopoietic and neuronal growth and development. Required to control retinal neovascularization in adult ocular endothelial cells. In complex with XRCC6 and XRCC5 (Ku80), up-regulates transcription from the osteocalcin promoter.
SUBUNIT: Interacts with NAA10, XRCC6, NAA50 and XRCC5.
SUBCELLULAR LOCATION: Cytoplasm. Nucleus. Note=Mainly cytoplasmic, nuclear in some cases. Present in the free cytosolic and cytoskeleton-bound polysomes, but not in the membrane-bound polysomes.
TISSUE SPECIFICITY: Expressed at high levels in testis and in ocular endothelial cells. Also found in brain (corpus callosum), heart, colon, bone marrow and at lower levels in most adult tissues, including thyroid, liver, pancreas, mammary and salivary glands, lung, ovary, urogenital system and upper gastrointestinal tract. Overexpressed in gastric cancer, in papillary thyroid carcinomas and in a Burkitt lymphoma cell line (Daudi). Specifically suppressed in abnormal proliferating blood vessels in eyes of patients with proliferative diabetic retinopathy.
PTM: Cleaved by caspases during apoptosis, resulting in a stable 35 kDa fragment.
SIMILARITY: Contains 8 TPR repeats.
SEQUENCE CAUTION: Sequence=AAH39818.1; Type=Miscellaneous discrepancy; Note=Contaminating sequence. Potential poly-A sequence;

-  Primer design for this transcript
 

Primer3Plus can design qPCR Primers that straddle exon-exon-junctions, which amplify only cDNA, not genomic DNA.
Click here to load the transcript sequence and exon structure into Primer3Plus

Exonprimer can design one pair of Sanger sequencing primers around every exon, located in non-genic sequence.
Click here to open Exonprimer with this transcript

To design primers for a non-coding sequence, zoom to a region of interest and select from the drop-down menu: View > In External Tools > Primer3


-  MalaCards Disease Associations
  MalaCards Gene Search: NAA15
Diseases sorted by gene-association score: ogden syndrome (18), retinitis pigmentosa 75 (9), epidermolysis bullosa dystrophica (6)

-  Comparative Toxicogenomics Database (CTD)
  The following chemicals interact with this gene           more ... click here to view the complete list

+  Common Gene Haplotype Alleles
  Press "+" in the title bar above to open this section.

-  RNA-Seq Expression Data from GTEx (53 Tissues, 570 Donors)
  Highest median expression: 11.57 RPKM in Cells - EBV-transformed lymphocytes
Total median expression: 190.49 RPKM



View in GTEx track of Genome Browser    View at GTEx portal     View GTEx Body Map

+  Microarray Expression Data
  Press "+" in the title bar above to open this section.

-  mRNA Secondary Structure of 3' and 5' UTRs
 
RegionFold EnergyBasesEnergy/Base
Display As
5' UTR -92.30256-0.361 Picture PostScript Text
3' UTR -687.202697-0.255 Picture PostScript Text

The RNAfold program from the Vienna RNA Package is used to perform the secondary structure predictions and folding calculations. The estimated folding energy is in kcal/mol. The more negative the energy, the more secondary structure the RNA is likely to have.

-  Protein Domain and Structure Information
  InterPro Domains: Graphical view of domain structure
IPR021183 - NatA_aux_su
IPR013026 - TPR-contain_dom
IPR011990 - TPR-like_helical
IPR013105 - TPR_2
IPR019734 - TPR_repeat

Pfam Domains:
PF12569 - NMDA receptor-regulated protein 1
PF13181 - Tetratricopeptide repeat

SCOP Domains:
81901 - HCP-like
48439 - Protein prenylyltransferase
48452 - TPR-like

ModBase Predicted Comparative 3D Structure on Q9BXJ9
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-  Orthologous Genes in Other Species
  Orthologies between human, mouse, and rat are computed by taking the best BLASTP hit, and filtering out non-syntenic hits. For more distant species reciprocal-best BLASTP hits are used. Note that the absence of an ortholog in the table below may reflect incomplete annotations in the other species rather than a true absence of the orthologous gene.
MouseRatZebrafishD. melanogasterC. elegansS. cerevisiae
No orthologNo orthologGenome BrowserGenome BrowserGenome BrowserGenome Browser
Gene Details  Gene DetailsGene DetailsGene Details
Gene Sorter  Gene SorterGene SorterGene Sorter
  EnsemblFlyBaseWormBaseSGD
  Protein SequenceProtein SequenceProtein SequenceProtein Sequence
  AlignmentAlignmentAlignmentAlignment

-  Gene Ontology (GO) Annotations with Structured Vocabulary
  Molecular Function:
GO:0003723 RNA binding
GO:0005515 protein binding
GO:0043022 ribosome binding
GO:0004596 peptide alpha-N-acetyltransferase activity
GO:0016407 acetyltransferase activity

Biological Process:
GO:0001525 angiogenesis
GO:0006351 transcription, DNA-templated
GO:0006355 regulation of transcription, DNA-templated
GO:0006474 N-terminal protein amino acid acetylation
GO:0007275 multicellular organism development
GO:0017196 N-terminal peptidyl-methionine acetylation
GO:0030154 cell differentiation
GO:0043066 negative regulation of apoptotic process
GO:0045893 positive regulation of transcription, DNA-templated
GO:0050821 protein stabilization

Cellular Component:
GO:0005634 nucleus
GO:0005667 transcription factor complex
GO:0005737 cytoplasm
GO:0005829 cytosol
GO:0016020 membrane
GO:0031415 NatA complex


-  Descriptions from all associated GenBank mRNAs
  BC039818 - Homo sapiens NMDA receptor regulated 1, mRNA (cDNA clone IMAGE:5499884), partial cds.
AK314626 - Homo sapiens cDNA, FLJ95468, highly similar to Homo sapiens transcriptional coactivator tubedown-100 (TBDN100),transcript variant 1, mRNA.
AK304417 - Homo sapiens cDNA FLJ58231 complete cds, highly similar to NMDA receptor-regulated protein 1.
AK023402 - Homo sapiens cDNA FLJ13340 fis, clone OVARC1001942, weakly similar to N-TERMINAL ACETYLTRANSFERASE 1 (EC 2.3.1.88).
AY039242 - Homo sapiens gastric cancer antigen Ga19 mRNA, complete cds.
AF327722 - Homo sapiens putative acetyltransferase mRNA, complete cds.
BC032642 - Homo sapiens NMDA receptor regulated 1, mRNA (cDNA clone IMAGE:5495731), partial cds.
AJ314788 - Homo sapiens mRNA for putative N-acetyltransferase.
BC093928 - Homo sapiens NMDA receptor regulated 1, mRNA (cDNA clone MGC:120963 IMAGE:7939773), complete cds.
BC104806 - Homo sapiens NMDA receptor regulated 1, mRNA (cDNA clone MGC:132466 IMAGE:8143809), complete cds.
AB528041 - Synthetic construct DNA, clone: pF1KB0434, Homo sapiens NARG1 gene for NMDA receptor regulated 1, without stop codon, in Flexi system.
KJ894785 - Synthetic construct Homo sapiens clone ccsbBroadEn_04179 NAA15 gene, encodes complete protein.
AY112670 - Homo sapiens transcriptional coactivator tubedown-100 (TBDN100) mRNA, complete cds.
AK023387 - Homo sapiens cDNA FLJ13325 fis, clone OVARC1001762, weakly similar to N-TERMINAL ACETYLTRANSFERASE 1 (EC 2.3.1.88).
JD225850 - Sequence 206874 from Patent EP1572962.
JD243695 - Sequence 224719 from Patent EP1572962.
JD347200 - Sequence 328224 from Patent EP1572962.
JD407188 - Sequence 388212 from Patent EP1572962.
JD460855 - Sequence 441879 from Patent EP1572962.
JD056109 - Sequence 37133 from Patent EP1572962.
JD223397 - Sequence 204421 from Patent EP1572962.
JD286285 - Sequence 267309 from Patent EP1572962.
JD286979 - Sequence 268003 from Patent EP1572962.
JD042123 - Sequence 23147 from Patent EP1572962.
AK001595 - Homo sapiens cDNA FLJ10733 fis, clone NT2RP3001392.
JD509967 - Sequence 490991 from Patent EP1572962.
JD402638 - Sequence 383662 from Patent EP1572962.
AK091308 - Homo sapiens cDNA FLJ33989 fis, clone DFNES2006944.
JD300031 - Sequence 281055 from Patent EP1572962.
JD511219 - Sequence 492243 from Patent EP1572962.
JD564069 - Sequence 545093 from Patent EP1572962.
JD244689 - Sequence 225713 from Patent EP1572962.
JD358056 - Sequence 339080 from Patent EP1572962.
JD410414 - Sequence 391438 from Patent EP1572962.
JD235121 - Sequence 216145 from Patent EP1572962.
JD285342 - Sequence 266366 from Patent EP1572962.
JD284457 - Sequence 265481 from Patent EP1572962.
JD043627 - Sequence 24651 from Patent EP1572962.
JD182400 - Sequence 163424 from Patent EP1572962.
JD344913 - Sequence 325937 from Patent EP1572962.
JD439185 - Sequence 420209 from Patent EP1572962.
JD301238 - Sequence 282262 from Patent EP1572962.
JD089779 - Sequence 70803 from Patent EP1572962.
JD350739 - Sequence 331763 from Patent EP1572962.
JD149766 - Sequence 130790 from Patent EP1572962.
JD346817 - Sequence 327841 from Patent EP1572962.
JD511351 - Sequence 492375 from Patent EP1572962.
JD090811 - Sequence 71835 from Patent EP1572962.
JD110048 - Sequence 91072 from Patent EP1572962.
JD503101 - Sequence 484125 from Patent EP1572962.
JD239356 - Sequence 220380 from Patent EP1572962.
JD263375 - Sequence 244399 from Patent EP1572962.
JD316331 - Sequence 297355 from Patent EP1572962.
JD268200 - Sequence 249224 from Patent EP1572962.
JD235277 - Sequence 216301 from Patent EP1572962.

-  Other Names for This Gene
  Alternate Gene Symbols: D3DNY6, GA19, NAA15_HUMAN, NARG1, NATH, NM_057175, NP_476516, Q52LG9, Q8IWH4, Q8NEV2, Q9BXJ9, Q9H8P6, TBDN100
UCSC ID: uc003ihu.1
RefSeq Accession: NM_057175
Protein: Q9BXJ9 (aka NAA15_HUMAN)
CCDS: CCDS43270.1

-  Gene Model Information
 
category: coding nonsense-mediated-decay: no RNA accession: NM_057175.3
exon count: 20CDS single in 3' UTR: no RNA size: 5554
ORF size: 2601CDS single in intron: no Alignment % ID: 100.00
txCdsPredict score: 5402.00frame shift in genome: no % Coverage: 100.00
has start codon: yes stop codon in genome: no # of Alignments: 1
has end codon: yes retained intron: no # AT/AC introns 0
selenocysteine: no end bleed into intron: 0# strange splices: 0
Click here for a detailed description of the fields of the table above.

-  Methods, Credits, and Use Restrictions
  Click here for details on how this gene model was made and data restrictions if any.