Human Gene UBE2C (uc002xpm.3)
  Description: Homo sapiens ubiquitin-conjugating enzyme E2C (UBE2C), transcript variant 1, mRNA.
RefSeq Summary (NM_007019): The modification of proteins with ubiquitin is an important cellular mechanism for targeting abnormal or short-lived proteins for degradation. Ubiquitination involves at least three classes of enzymes: ubiquitin-activating enzymes, ubiquitin-conjugating enzymes, and ubiquitin-protein ligases. This gene encodes a member of the E2 ubiquitin-conjugating enzyme family. The encoded protein is required for the destruction of mitotic cyclins and for cell cycle progression, and may be involved in cancer progression. Multiple transcript variants encoding different isoforms have been found for this gene. Pseudogenes of this gene have been defined on chromosomes 4, 14, 15, 18, and 19. [provided by RefSeq, Aug 2013].
Transcript (Including UTRs)
   Position: hg19 chr20:44,441,255-44,445,596 Size: 4,342 Total Exon Count: 6 Strand: +
Coding Region
   Position: hg19 chr20:44,441,335-44,445,406 Size: 4,072 Coding Exon Count: 6 

Page IndexSequence and LinksUniProtKB CommentsPrimersGenetic AssociationsCTD
Gene AllelesRNA-Seq ExpressionMicroarray ExpressionRNA StructureProtein StructureOther Species
GO AnnotationsmRNA DescriptionsPathwaysOther NamesModel InformationMethods
Data last updated at UCSC: 2013-06-14

-  Sequence and Links to Tools and Databases
 
Genomic Sequence (chr20:44,441,255-44,445,596)mRNA (may differ from genome)Protein (179 aa)
Gene SorterGenome BrowserOther Species FASTAVisiGeneGene interactionsTable Schema
AlphaFoldBioGPSEnsemblEntrez GeneExonPrimerGeneCards
GeneNetworkH-INVHGNCHPRDLynxMGI
neXtProtOMIMPubMedReactomeTreefamUniProtKB
WikipediaBioGrid CRISPR DB

-  Comments and Description Text from UniProtKB
  ID: UBE2C_HUMAN
DESCRIPTION: RecName: Full=Ubiquitin-conjugating enzyme E2 C; EC=6.3.2.19; AltName: Full=UbcH10; AltName: Full=Ubiquitin carrier protein C; AltName: Full=Ubiquitin-protein ligase C;
FUNCTION: Accepts ubiquitin from the E1 complex and catalyzes its covalent attachment to other proteins. In vitro catalyzes 'Lys- 11'- and 'Lys-48'-linked polyubiquitination. Acts as an essential factor of the anaphase promoting complex/cyclosome (APC/C), a cell cycle-regulated ubiquitin ligase that controls progression through mitosis. Acts by initiating 'Lys-11'-linked polyubiquitin chains on APC/C substrates, leading to the degradation of APC/C substrates by the proteasome and promoting mitotic exit.
CATALYTIC ACTIVITY: ATP + ubiquitin + protein lysine = AMP + diphosphate + protein N-ubiquityllysine.
PATHWAY: Protein modification; protein ubiquitination.
SUBUNIT: Component of the APC/C complex.
PTM: Autoubiquitinated by the APC/C complex, leading to its degradation by the proteasome. Its degradation plays a central role in APC/C regulation, allowing cyclin-A accumulation before S phase entry. APC/C substrates inhibit the autoubiquitination of UBE2C/UBCH10 but not its E2 function, hence APC/C remaining active until its substrates have been destroyed.
SIMILARITY: Belongs to the ubiquitin-conjugating enzyme family.
WEB RESOURCE: Name=Atlas of Genetics and Cytogenetics in Oncology and Haematology; URL="http://atlasgeneticsoncology.org/Genes/UBE2CID44079ch20q13.html";

-  Primer design for this transcript
 

Primer3Plus can design qPCR Primers that straddle exon-exon-junctions, which amplify only cDNA, not genomic DNA.
Click here to load the transcript sequence and exon structure into Primer3Plus

Exonprimer can design one pair of Sanger sequencing primers around every exon, located in non-genic sequence.
Click here to open Exonprimer with this transcript

To design primers for a non-coding sequence, zoom to a region of interest and select from the drop-down menu: View > In External Tools > Primer3


-  Genetic Association Studies of Complex Diseases and Disorders
  Genetic Association Database (archive): UBE2C
CDC HuGE Published Literature: UBE2C

-  Comparative Toxicogenomics Database (CTD)
  The following chemicals interact with this gene           more ... click here to view the complete list

+  Common Gene Haplotype Alleles
  Press "+" in the title bar above to open this section.

-  RNA-Seq Expression Data from GTEx (53 Tissues, 570 Donors)
  Highest median expression: 45.10 RPKM in Cells - EBV-transformed lymphocytes
Total median expression: 110.03 RPKM



View in GTEx track of Genome Browser    View at GTEx portal     View GTEx Body Map

+  Microarray Expression Data
  Press "+" in the title bar above to open this section.

-  mRNA Secondary Structure of 3' and 5' UTRs
 
RegionFold EnergyBasesEnergy/Base
Display As
5' UTR -30.8080-0.385 Picture PostScript Text
3' UTR -31.99190-0.168 Picture PostScript Text

The RNAfold program from the Vienna RNA Package is used to perform the secondary structure predictions and folding calculations. The estimated folding energy is in kcal/mol. The more negative the energy, the more secondary structure the RNA is likely to have.

-  Protein Domain and Structure Information
  InterPro Domains: Graphical view of domain structure
IPR000608 - UBQ-conjugat_E2
IPR023313 - UBQ-conjugating_AS
IPR016135 - UBQ-conjugating_enzyme/RWD

Pfam Domains:
PF00179 - Ubiquitin-conjugating enzyme

SCOP Domains:
54495 - UBC-like

Protein Data Bank (PDB) 3-D Structure
MuPIT help
1I7K - X-ray MuPIT


ModBase Predicted Comparative 3D Structure on O00762
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The pictures above may be empty if there is no ModBase structure for the protein. The ModBase structure frequently covers just a fragment of the protein. You may be asked to log onto ModBase the first time you click on the pictures. It is simplest after logging in to just click on the picture again to get to the specific info on that model.

-  Orthologous Genes in Other Species
  Orthologies between human, mouse, and rat are computed by taking the best BLASTP hit, and filtering out non-syntenic hits. For more distant species reciprocal-best BLASTP hits are used. Note that the absence of an ortholog in the table below may reflect incomplete annotations in the other species rather than a true absence of the orthologous gene.
MouseRatZebrafishD. melanogasterC. elegansS. cerevisiae
No orthologNo orthologGenome BrowserGenome BrowserNo orthologGenome Browser
Gene Details  Gene Details Gene Details
Gene Sorter  Gene Sorter Gene Sorter
  EnsemblFlyBase SGD
  Protein SequenceProtein Sequence Protein Sequence
  AlignmentAlignment Alignment

-  Gene Ontology (GO) Annotations with Structured Vocabulary
  Molecular Function:
GO:0000166 nucleotide binding
GO:0004842 ubiquitin-protein transferase activity
GO:0005515 protein binding
GO:0005524 ATP binding
GO:0016740 transferase activity
GO:0031625 ubiquitin protein ligase binding
GO:0044389 ubiquitin-like protein ligase binding
GO:0061630 ubiquitin protein ligase activity
GO:0061631 ubiquitin conjugating enzyme activity

Biological Process:
GO:0006511 ubiquitin-dependent protein catabolic process
GO:0007049 cell cycle
GO:0010458 exit from mitosis
GO:0010994 free ubiquitin chain polymerization
GO:0016567 protein ubiquitination
GO:0030071 regulation of mitotic metaphase/anaphase transition
GO:0031145 anaphase-promoting complex-dependent catabolic process
GO:0031536 positive regulation of exit from mitosis
GO:0051301 cell division
GO:0070936 protein K48-linked ubiquitination
GO:0070979 protein K11-linked ubiquitination
GO:1904668 positive regulation of ubiquitin protein ligase activity

Cellular Component:
GO:0000151 ubiquitin ligase complex
GO:0005654 nucleoplasm
GO:0005680 anaphase-promoting complex
GO:0005737 cytoplasm
GO:0005829 cytosol
GO:0005886 plasma membrane


-  Descriptions from all associated GenBank mRNAs
  AK311949 - Homo sapiens cDNA, FLJ92217, highly similar to Homo sapiens ubiquitin-conjugating enzyme E2C (UBE2C), mRNA.
LF383769 - JP 2014500723-A/191272: Polycomb-Associated Non-Coding RNAs.
BC050736 - Homo sapiens ubiquitin-conjugating enzyme E2C, mRNA (cDNA clone MGC:60380 IMAGE:6503915), complete cds.
BC016292 - Homo sapiens ubiquitin-conjugating enzyme E2C, mRNA (cDNA clone MGC:9617 IMAGE:3910286), complete cds.
BC007656 - Homo sapiens ubiquitin-conjugating enzyme E2C, mRNA (cDNA clone MGC:1234 IMAGE:3345575), complete cds.
U73379 - Human cyclin-selective ubiquitin carrier protein mRNA, complete cds.
BC028161 - Homo sapiens ubiquitin-conjugating enzyme E2C, mRNA (cDNA clone IMAGE:5228503).
CR456988 - Homo sapiens full open reading frame cDNA clone RZPDo834D115D for gene UBE2C, ubiquitin-conjugating enzyme E2C; complete cds, incl. stopcodon.
EU831714 - Synthetic construct Homo sapiens clone HAIB:100066743; DKFZo003E0220 ubiquitin-conjugating enzyme E2C protein (UBE2C) gene, encodes complete protein.
BT007300 - Homo sapiens ubiquitin-conjugating enzyme E2C mRNA, complete cds.
BT020165 - Homo sapiens ubiquitin-conjugating enzyme E2C mRNA, complete cds.
BT020166 - Homo sapiens ubiquitin-conjugating enzyme E2C mRNA, complete cds.
EU831629 - Synthetic construct Homo sapiens clone HAIB:100066658; DKFZo007E0219 ubiquitin-conjugating enzyme E2C protein (UBE2C) gene, encodes complete protein.
DQ892416 - Synthetic construct clone IMAGE:100005046; FLH186150.01X; RZPDo839B0671D ubiquitin-conjugating enzyme E2C (UBE2C) gene, encodes complete protein.
DQ895630 - Synthetic construct Homo sapiens clone IMAGE:100010090; FLH186146.01L; RZPDo839B0661D ubiquitin-conjugating enzyme E2C (UBE2C) gene, encodes complete protein.
LF350572 - JP 2014500723-A/158075: Polycomb-Associated Non-Coding RNAs.
LF350575 - JP 2014500723-A/158078: Polycomb-Associated Non-Coding RNAs.
JD200207 - Sequence 181231 from Patent EP1572962.
JD091289 - Sequence 72313 from Patent EP1572962.
MA619346 - JP 2018138019-A/191272: Polycomb-Associated Non-Coding RNAs.
MA586149 - JP 2018138019-A/158075: Polycomb-Associated Non-Coding RNAs.
MA586152 - JP 2018138019-A/158078: Polycomb-Associated Non-Coding RNAs.

-  Biochemical and Signaling Pathways
  KEGG - Kyoto Encyclopedia of Genes and Genomes
hsa04120 - Ubiquitin mediated proteolysis

Reactome (by CSHL, EBI, and GO)

Protein O00762 (Reactome details) participates in the following event(s):

R-HSA-174119 Free APC/C phosphorylated by Plk1
R-HSA-174132 Free APC/C phosphorylated by Cyclin B:Cdc2
R-HSA-174139 Dissociation of phospho-Cdh1 from the APC/C complex
R-HSA-141423 Binding of the MCC complex to the APC/C complex
R-HSA-174070 Association of Cdh1 with the APC/C
R-HSA-174224 Dissociation of Cdc20 from APC/C complex
R-HSA-174088 Association of cell cycle proteins with the APC/C:Cdh1 complex
R-HSA-8852129 UBA1 conjugates ubiquitin to cytosolic E2 enzymes
R-HSA-983152 Transfer of ubiquitin from E1 to E2
R-HSA-983140 Transfer of Ub from E2 to substrate and release of E2
R-HSA-8852130 UBA1 conjugates ubiquitin to nuclear E2 enzymes
R-HSA-174057 Multiubiquitination of APC/C-associated Cdh1
R-HSA-188371 Association of Cyclin A:Cdk2 with Cdh1
R-HSA-3788708 CDKN1A (p21) prevents association of Cyclin A:Cdk2 with Cdh1
R-HSA-3788725 Cdh1:APC/C complex binds EHMT1:EHMT2
R-HSA-174171 Association of Cyclin A with the APC/C
R-HSA-174238 Activation of APC/C:Cdc20 by dissociation of Cdc20:phospho-APC/C from Cdc20:phospho-APC/C:Mad2:Bub3:BubR1
R-HSA-179410 Association of Nek2A with MCC:APC/C
R-HSA-174120 Association of Cyclin B:Cdc2 with Cdc20:APC/C complex
R-HSA-174121 Association of Securin with Cdc20:APC/C complex
R-HSA-174195 Ubiquitination of cell cycle proteins targeted by the APC/C:Cdh1complex
R-HSA-983157 Interaction of E3 with substrate and E2-Ub complex
R-HSA-174079 Phosphorylation of Cdh1 by Cyclin A:Cdk2
R-HSA-3788705 CDKN1A (p21) prevents phosphorylation of Cdh1 by Cyclin A:Cdk2
R-HSA-3788724 Cdh1:APC/C ubiquitinates EHMT1 and EHMT2
R-HSA-174104 Ubiquitination of Cyclin A by APC/C:Cdc20 complex
R-HSA-179417 Multiubiquitination of Nek2A
R-HSA-174227 Ubiquitination of Cyclin B by phospho-APC/C:Cdc20 complex
R-HSA-174144 Ubiquitination of Securin by phospho-APC/C:Cdc20 complex
R-HSA-69015 Cytoplasmic phosphorylated Cdc6 is ubiquitinated by the anaphase-promoting complex
R-HSA-176412 Phosphorylation of the APC/C
R-HSA-174084 Autodegradation of Cdh1 by Cdh1:APC/C
R-HSA-176408 Regulation of APC/C activators between G1/S and early anaphase
R-HSA-141430 Inactivation of APC/C via direct inhibition of the APC/C complex
R-HSA-176407 Conversion from APC/C:Cdc20 to APC/C:Cdh1 in late anaphase
R-HSA-174178 APC/C:Cdh1 mediated degradation of Cdc20 and other APC/C:Cdh1 targeted proteins in late mitosis/early G1
R-HSA-8866652 Synthesis of active ubiquitin: roles of E1 and E2 enzymes
R-HSA-983168 Antigen processing: Ubiquitination & Proteasome degradation
R-HSA-176814 Activation of APC/C and APC/C:Cdc20 mediated degradation of mitotic proteins
R-HSA-2559582 Senescence-Associated Secretory Phenotype (SASP)
R-HSA-174143 APC/C-mediated degradation of cell cycle proteins
R-HSA-174184 Cdc20:Phospho-APC/C mediated degradation of Cyclin A
R-HSA-176409 APC/C:Cdc20 mediated degradation of mitotic proteins
R-HSA-179409 APC-Cdc20 mediated degradation of Nek2A
R-HSA-174048 APC/C:Cdc20 mediated degradation of Cyclin B
R-HSA-174154 APC/C:Cdc20 mediated degradation of Securin
R-HSA-2467813 Separation of Sister Chromatids
R-HSA-141405 Inhibition of the proteolytic activity of APC/C required for the onset of anaphase by mitotic spindle checkpoint components
R-HSA-8852135 Protein ubiquitination
R-HSA-983169 Class I MHC mediated antigen processing & presentation
R-HSA-2559583 Cellular Senescence
R-HSA-453276 Regulation of mitotic cell cycle
R-HSA-179419 APC:Cdc20 mediated degradation of cell cycle proteins prior to satisfation of the cell cycle checkpoint
R-HSA-68882 Mitotic Anaphase
R-HSA-69017 CDK-mediated phosphorylation and removal of Cdc6
R-HSA-69618 Mitotic Spindle Checkpoint
R-HSA-597592 Post-translational protein modification
R-HSA-1280218 Adaptive Immune System
R-HSA-2262752 Cellular responses to stress
R-HSA-69278 Cell Cycle (Mitotic)
R-HSA-2555396 Mitotic Metaphase and Anaphase
R-HSA-69052 Switching of origins to a post-replicative state
R-HSA-69620 Cell Cycle Checkpoints
R-HSA-392499 Metabolism of proteins
R-HSA-168256 Immune System
R-HSA-8953897 Cellular responses to external stimuli
R-HSA-1640170 Cell Cycle
R-HSA-68886 M Phase
R-HSA-69239 Synthesis of DNA
R-HSA-69242 S Phase
R-HSA-69306 DNA Replication

-  Other Names for This Gene
  Alternate Gene Symbols: NM_007019, NP_861518, O00762, UBCH10, UBE2C_HUMAN
UCSC ID: uc002xpm.3
RefSeq Accession: NM_007019
Protein: O00762 (aka UBE2C_HUMAN or UBCC_HUMAN)
CCDS: CCDS13370.1

-  Gene Model Information
 
category: coding nonsense-mediated-decay: no RNA accession: NM_007019.2
exon count: 6CDS single in 3' UTR: no RNA size: 823
ORF size: 540CDS single in intron: no Alignment % ID: 100.00
txCdsPredict score: 1280.00frame shift in genome: no % Coverage: 98.42
has start codon: yes stop codon in genome: no # of Alignments: 1
has end codon: yes retained intron: no # AT/AC introns 0
selenocysteine: no end bleed into intron: 0# strange splices: 0
Click here for a detailed description of the fields of the table above.

-  Methods, Credits, and Use Restrictions
  Click here for details on how this gene model was made and data restrictions if any.