Description: Homo sapiens ubiquitin-conjugating enzyme E2C (UBE2C), transcript variant 1, mRNA. RefSeq Summary (NM_007019): The modification of proteins with ubiquitin is an important cellular mechanism for targeting abnormal or short-lived proteins for degradation. Ubiquitination involves at least three classes of enzymes: ubiquitin-activating enzymes, ubiquitin-conjugating enzymes, and ubiquitin-protein ligases. This gene encodes a member of the E2 ubiquitin-conjugating enzyme family. The encoded protein is required for the destruction of mitotic cyclins and for cell cycle progression, and may be involved in cancer progression. Multiple transcript variants encoding different isoforms have been found for this gene. Pseudogenes of this gene have been defined on chromosomes 4, 14, 15, 18, and 19. [provided by RefSeq, Aug 2013]. Transcript (Including UTRs) Position: hg19 chr20:44,441,255-44,445,596 Size: 4,342 Total Exon Count: 6 Strand: + Coding Region Position: hg19 chr20:44,441,335-44,445,406 Size: 4,072 Coding Exon Count: 6
ID:UBE2C_HUMAN DESCRIPTION: RecName: Full=Ubiquitin-conjugating enzyme E2 C; EC=6.3.2.19; AltName: Full=UbcH10; AltName: Full=Ubiquitin carrier protein C; AltName: Full=Ubiquitin-protein ligase C; FUNCTION: Accepts ubiquitin from the E1 complex and catalyzes its covalent attachment to other proteins. In vitro catalyzes 'Lys- 11'- and 'Lys-48'-linked polyubiquitination. Acts as an essential factor of the anaphase promoting complex/cyclosome (APC/C), a cell cycle-regulated ubiquitin ligase that controls progression through mitosis. Acts by initiating 'Lys-11'-linked polyubiquitin chains on APC/C substrates, leading to the degradation of APC/C substrates by the proteasome and promoting mitotic exit. CATALYTIC ACTIVITY: ATP + ubiquitin + protein lysine = AMP + diphosphate + protein N-ubiquityllysine. PATHWAY: Protein modification; protein ubiquitination. SUBUNIT: Component of the APC/C complex. PTM: Autoubiquitinated by the APC/C complex, leading to its degradation by the proteasome. Its degradation plays a central role in APC/C regulation, allowing cyclin-A accumulation before S phase entry. APC/C substrates inhibit the autoubiquitination of UBE2C/UBCH10 but not its E2 function, hence APC/C remaining active until its substrates have been destroyed. SIMILARITY: Belongs to the ubiquitin-conjugating enzyme family. WEB RESOURCE: Name=Atlas of Genetics and Cytogenetics in Oncology and Haematology; URL="http://atlasgeneticsoncology.org/Genes/UBE2CID44079ch20q13.html";
The RNAfold program from the Vienna RNA Package is used to perform the secondary structure predictions and folding calculations. The estimated folding energy is in kcal/mol. The more negative the energy, the more secondary structure the RNA is likely to have.
ModBase Predicted Comparative 3D Structure on O00762
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Orthologous Genes in Other Species
Orthologies between human, mouse, and rat are computed by taking the best BLASTP hit, and filtering out non-syntenic hits. For more distant species reciprocal-best BLASTP hits are used. Note that the absence of an ortholog in the table below may reflect incomplete annotations in the other species rather than a true absence of the orthologous gene.
Gene Ontology (GO) Annotations with Structured Vocabulary
Molecular Function: GO:0000166 nucleotide binding GO:0004842 ubiquitin-protein transferase activity GO:0005515 protein binding GO:0005524 ATP binding GO:0016740 transferase activity GO:0031625 ubiquitin protein ligase binding GO:0044389 ubiquitin-like protein ligase binding GO:0061630 ubiquitin protein ligase activity GO:0061631 ubiquitin conjugating enzyme activity
Biological Process: GO:0006511 ubiquitin-dependent protein catabolic process GO:0007049 cell cycle GO:0010458 exit from mitosis GO:0010994 free ubiquitin chain polymerization GO:0016567 protein ubiquitination GO:0030071 regulation of mitotic metaphase/anaphase transition GO:0031145 anaphase-promoting complex-dependent catabolic process GO:0031536 positive regulation of exit from mitosis GO:0051301 cell division GO:0070936 protein K48-linked ubiquitination GO:0070979 protein K11-linked ubiquitination GO:1904668 positive regulation of ubiquitin protein ligase activity
KEGG - Kyoto Encyclopedia of Genes and Genomes hsa04120 - Ubiquitin mediated proteolysis
Reactome (by CSHL, EBI, and GO)
Protein O00762 (Reactome details) participates in the following event(s):
R-HSA-174119 Free APC/C phosphorylated by Plk1 R-HSA-174132 Free APC/C phosphorylated by Cyclin B:Cdc2 R-HSA-174139 Dissociation of phospho-Cdh1 from the APC/C complex R-HSA-141423 Binding of the MCC complex to the APC/C complex R-HSA-174070 Association of Cdh1 with the APC/C R-HSA-174224 Dissociation of Cdc20 from APC/C complex R-HSA-174088 Association of cell cycle proteins with the APC/C:Cdh1 complex R-HSA-8852129 UBA1 conjugates ubiquitin to cytosolic E2 enzymes R-HSA-983152 Transfer of ubiquitin from E1 to E2 R-HSA-983140 Transfer of Ub from E2 to substrate and release of E2 R-HSA-8852130 UBA1 conjugates ubiquitin to nuclear E2 enzymes R-HSA-174057 Multiubiquitination of APC/C-associated Cdh1 R-HSA-188371 Association of Cyclin A:Cdk2 with Cdh1 R-HSA-3788708 CDKN1A (p21) prevents association of Cyclin A:Cdk2 with Cdh1 R-HSA-3788725 Cdh1:APC/C complex binds EHMT1:EHMT2 R-HSA-174171 Association of Cyclin A with the APC/C R-HSA-174238 Activation of APC/C:Cdc20 by dissociation of Cdc20:phospho-APC/C from Cdc20:phospho-APC/C:Mad2:Bub3:BubR1 R-HSA-179410 Association of Nek2A with MCC:APC/C R-HSA-174120 Association of Cyclin B:Cdc2 with Cdc20:APC/C complex R-HSA-174121 Association of Securin with Cdc20:APC/C complex R-HSA-174195 Ubiquitination of cell cycle proteins targeted by the APC/C:Cdh1complex R-HSA-983157 Interaction of E3 with substrate and E2-Ub complex R-HSA-174079 Phosphorylation of Cdh1 by Cyclin A:Cdk2 R-HSA-3788705 CDKN1A (p21) prevents phosphorylation of Cdh1 by Cyclin A:Cdk2 R-HSA-3788724 Cdh1:APC/C ubiquitinates EHMT1 and EHMT2 R-HSA-174104 Ubiquitination of Cyclin A by APC/C:Cdc20 complex R-HSA-179417 Multiubiquitination of Nek2A R-HSA-174227 Ubiquitination of Cyclin B by phospho-APC/C:Cdc20 complex R-HSA-174144 Ubiquitination of Securin by phospho-APC/C:Cdc20 complex R-HSA-69015 Cytoplasmic phosphorylated Cdc6 is ubiquitinated by the anaphase-promoting complex R-HSA-176412 Phosphorylation of the APC/C R-HSA-174084 Autodegradation of Cdh1 by Cdh1:APC/C R-HSA-176408 Regulation of APC/C activators between G1/S and early anaphase R-HSA-141430 Inactivation of APC/C via direct inhibition of the APC/C complex R-HSA-176407 Conversion from APC/C:Cdc20 to APC/C:Cdh1 in late anaphase R-HSA-174178 APC/C:Cdh1 mediated degradation of Cdc20 and other APC/C:Cdh1 targeted proteins in late mitosis/early G1 R-HSA-8866652 Synthesis of active ubiquitin: roles of E1 and E2 enzymes R-HSA-983168 Antigen processing: Ubiquitination & Proteasome degradation R-HSA-176814 Activation of APC/C and APC/C:Cdc20 mediated degradation of mitotic proteins R-HSA-2559582 Senescence-Associated Secretory Phenotype (SASP) R-HSA-174143 APC/C-mediated degradation of cell cycle proteins R-HSA-174184 Cdc20:Phospho-APC/C mediated degradation of Cyclin A R-HSA-176409 APC/C:Cdc20 mediated degradation of mitotic proteins R-HSA-179409 APC-Cdc20 mediated degradation of Nek2A R-HSA-174048 APC/C:Cdc20 mediated degradation of Cyclin B R-HSA-174154 APC/C:Cdc20 mediated degradation of Securin R-HSA-2467813 Separation of Sister Chromatids R-HSA-141405 Inhibition of the proteolytic activity of APC/C required for the onset of anaphase by mitotic spindle checkpoint components R-HSA-8852135 Protein ubiquitination R-HSA-983169 Class I MHC mediated antigen processing & presentation R-HSA-2559583 Cellular Senescence R-HSA-453276 Regulation of mitotic cell cycle R-HSA-179419 APC:Cdc20 mediated degradation of cell cycle proteins prior to satisfation of the cell cycle checkpoint R-HSA-68882 Mitotic Anaphase R-HSA-69017 CDK-mediated phosphorylation and removal of Cdc6 R-HSA-69618 Mitotic Spindle Checkpoint R-HSA-597592 Post-translational protein modification R-HSA-1280218 Adaptive Immune System R-HSA-2262752 Cellular responses to stress R-HSA-69278 Cell Cycle (Mitotic) R-HSA-2555396 Mitotic Metaphase and Anaphase R-HSA-69052 Switching of origins to a post-replicative state R-HSA-69620 Cell Cycle Checkpoints R-HSA-392499 Metabolism of proteins R-HSA-168256 Immune System R-HSA-8953897 Cellular responses to external stimuli R-HSA-1640170 Cell Cycle R-HSA-68886 M Phase R-HSA-69239 Synthesis of DNA R-HSA-69242 S Phase R-HSA-69306 DNA Replication