Human Gene MAN1A2 (ENST00000356554.7) from GENCODE V44
  Description: Homo sapiens mannosidase alpha class 1A member 2 (MAN1A2), mRNA. (from RefSeq NM_006699)
RefSeq Summary (NM_006699): Alpha-mannosidases function at different stages of N-glycan maturation in mammalian cells. See MAN2A1 (MIM 154582) for general information.[supplied by OMIM, Mar 2008]. Sequence Note: This RefSeq record was created from transcript and genomic sequence data to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on transcript alignments.
Gencode Transcript: ENST00000356554.7
Gencode Gene: ENSG00000198162.12
Transcript (Including UTRs)
   Position: hg38 chr1:117,367,449-117,528,872 Size: 161,424 Total Exon Count: 13 Strand: +
Coding Region
   Position: hg38 chr1:117,368,184-117,522,957 Size: 154,774 Coding Exon Count: 13 

Page IndexSequence and LinksUniProtKB CommentsPrimersCTDRNA-Seq Expression
Microarray ExpressionRNA StructureProtein StructureOther SpeciesGO AnnotationsmRNA Descriptions
PathwaysOther NamesMethods
Data last updated at UCSC: 2023-08-18 00:09:47

-  Sequence and Links to Tools and Databases
 
Genomic Sequence (chr1:117,367,449-117,528,872)mRNA (may differ from genome)Protein (641 aa)
Gene SorterGenome BrowserOther Species FASTAGene interactionsTable SchemaAlphaFold
BioGPSEnsemblEntrez GeneExonPrimerGencodeGeneCards
HGNCHPRDLynxMGIneXtProtOMIM
PubMedReactomeUniProtKBWikipediaBioGrid CRISPR DB

-  Comments and Description Text from UniProtKB
  ID: MA1A2_HUMAN
DESCRIPTION: RecName: Full=Mannosyl-oligosaccharide 1,2-alpha-mannosidase IB; EC=3.2.1.113; AltName: Full=Mannosidase alpha class 1A member 2; AltName: Full=Processing alpha-1,2-mannosidase IB; Short=Alpha-1,2-mannosidase IB;
FUNCTION: Involved in the maturation of Asn-linked oligosaccharides. Progressively trim alpha-1,2-linked mannose residues from Man(9)GlcNAc(2) to produce Man(5)GlcNAc(2).
CATALYTIC ACTIVITY: Hydrolysis of the terminal (1->2)-linked alpha-D-mannose residues in the oligo-mannose oligosaccharide Man(9)(GlcNAc)(2).
COFACTOR: Calcium.
ENZYME REGULATION: Inhibited by both 1-deoxymannojirimycin and kifunensine.
PATHWAY: Protein modification; protein glycosylation.
SUBCELLULAR LOCATION: Golgi apparatus membrane; Single-pass type II membrane protein.
TISSUE SPECIFICITY: Highest levels of expression in placenta and testis.
SIMILARITY: Belongs to the glycosyl hydrolase 47 family.

-  Primer design for this transcript
 

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Click here to load the transcript sequence and exon structure into Primer3Plus

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-  Comparative Toxicogenomics Database (CTD)
  The following chemicals interact with this gene           more ... click here to view the complete list

-  RNA-Seq Expression Data from GTEx (53 Tissues, 570 Donors)
  Highest median expression: 7.93 RPKM in Fallopian Tube
Total median expression: 273.09 RPKM



View in GTEx track of Genome Browser    View at GTEx portal     View GTEx Body Map

+  Microarray Expression Data
  Press "+" in the title bar above to open this section.

-  mRNA Secondary Structure of 3' and 5' UTRs
 
RegionFold EnergyBasesEnergy/Base
Display As
5' UTR -260.90735-0.355 Picture PostScript Text
3' UTR -1369.105915-0.231 Picture PostScript Text

The RNAfold program from the Vienna RNA Package is used to perform the secondary structure predictions and folding calculations. The estimated folding energy is in kcal/mol. The more negative the energy, the more secondary structure the RNA is likely to have.

-  Protein Domain and Structure Information
  InterPro Domains: Graphical view of domain structure
IPR001382 - Glyco_hydro_47

Pfam Domains:
PF01532 - Glycosyl hydrolase family 47

ModBase Predicted Comparative 3D Structure on O60476
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-  Orthologous Genes in Other Species
  Orthologies between human, mouse, and rat are computed by taking the best BLASTP hit, and filtering out non-syntenic hits. For more distant species reciprocal-best BLASTP hits are used. Note that the absence of an ortholog in the table below may reflect incomplete annotations in the other species rather than a true absence of the orthologous gene.
MouseRatZebrafishD. melanogasterC. elegansS. cerevisiae
Genome BrowserGenome BrowserGenome BrowserGenome BrowserGenome BrowserNo ortholog
Gene Details     
Gene Sorter     
MGIRGDEnsemblEnsemblWormBase 
Protein SequenceProtein SequenceProtein SequenceProtein SequenceProtein Sequence 
AlignmentAlignmentAlignmentAlignmentAlignment 

-  Gene Ontology (GO) Annotations with Structured Vocabulary
  Molecular Function:
GO:0003824 catalytic activity
GO:0004571 mannosyl-oligosaccharide 1,2-alpha-mannosidase activity
GO:0005509 calcium ion binding
GO:0016787 hydrolase activity
GO:0016798 hydrolase activity, acting on glycosyl bonds
GO:0046872 metal ion binding

Biological Process:
GO:0006486 protein glycosylation
GO:0006491 N-glycan processing
GO:0007585 respiratory gaseous exchange
GO:0008152 metabolic process
GO:0009100 glycoprotein metabolic process
GO:0048286 lung alveolus development
GO:1904381 Golgi apparatus mannose trimming

Cellular Component:
GO:0000139 Golgi membrane
GO:0005783 endoplasmic reticulum
GO:0005794 Golgi apparatus
GO:0016020 membrane
GO:0016021 integral component of membrane
GO:0070062 extracellular exosome


-  Descriptions from all associated GenBank mRNAs
  BC052954 - Homo sapiens mannosidase, alpha, class 1A, member 2, mRNA (cDNA clone IMAGE:5264139), partial cds.
LF383818 - JP 2014500723-A/191321: Polycomb-Associated Non-Coding RNAs.
MA619395 - JP 2018138019-A/191321: Polycomb-Associated Non-Coding RNAs.
BC063300 - Homo sapiens mannosidase, alpha, class 1A, member 2, mRNA (cDNA clone MGC:71683 IMAGE:5263292), complete cds.
AF027156 - Homo sapiens alpha 1,2-mannosidase IB mRNA, complete cds.
AK023308 - Homo sapiens cDNA FLJ13246 fis, clone OVARC1000682, highly similar to PROCESSING ALPHA-1,2-MANNOSIDASE (EC 3.2.1.-).
AB385126 - Synthetic construct DNA, clone: pF1KB5584, Homo sapiens MAN1A2 gene for mannosyl-oligosaccharide 1,2-alpha-mannosidase IB, complete cds, without stop codon, in Flexi system.
AK001970 - Homo sapiens cDNA FLJ11108 fis, clone PLACE1005804, highly similar to Mannosyl-oligosaccharide1,2-alpha-mannosidase IB (EC 3.2.1.113).
AK307842 - Homo sapiens cDNA, FLJ97790.
JD313604 - Sequence 294628 from Patent EP1572962.
JD437378 - Sequence 418402 from Patent EP1572962.
JD061816 - Sequence 42840 from Patent EP1572962.
LF352752 - JP 2014500723-A/160255: Polycomb-Associated Non-Coding RNAs.
MA588329 - JP 2018138019-A/160255: Polycomb-Associated Non-Coding RNAs.
LF352751 - JP 2014500723-A/160254: Polycomb-Associated Non-Coding RNAs.
MA588328 - JP 2018138019-A/160254: Polycomb-Associated Non-Coding RNAs.
LF352748 - JP 2014500723-A/160251: Polycomb-Associated Non-Coding RNAs.
MA588325 - JP 2018138019-A/160251: Polycomb-Associated Non-Coding RNAs.
LF352747 - JP 2014500723-A/160250: Polycomb-Associated Non-Coding RNAs.
MA588324 - JP 2018138019-A/160250: Polycomb-Associated Non-Coding RNAs.
LF352745 - JP 2014500723-A/160248: Polycomb-Associated Non-Coding RNAs.
MA588322 - JP 2018138019-A/160248: Polycomb-Associated Non-Coding RNAs.
LF352742 - JP 2014500723-A/160245: Polycomb-Associated Non-Coding RNAs.
MA588319 - JP 2018138019-A/160245: Polycomb-Associated Non-Coding RNAs.
HQ234305 - Homo sapiens clone Man_1A2_E6-E2 MAN1A2 mRNA, partial sequence.
LF352730 - JP 2014500723-A/160233: Polycomb-Associated Non-Coding RNAs.
MA588307 - JP 2018138019-A/160233: Polycomb-Associated Non-Coding RNAs.
LF352729 - JP 2014500723-A/160232: Polycomb-Associated Non-Coding RNAs.
MA588306 - JP 2018138019-A/160232: Polycomb-Associated Non-Coding RNAs.
JD373866 - Sequence 354890 from Patent EP1572962.
JD522656 - Sequence 503680 from Patent EP1572962.
LF352726 - JP 2014500723-A/160229: Polycomb-Associated Non-Coding RNAs.
MA588303 - JP 2018138019-A/160229: Polycomb-Associated Non-Coding RNAs.
LF352725 - JP 2014500723-A/160228: Polycomb-Associated Non-Coding RNAs.
MA588302 - JP 2018138019-A/160228: Polycomb-Associated Non-Coding RNAs.
AK090840 - Homo sapiens cDNA FLJ33521 fis, clone BRAMY2006366.
LF352724 - JP 2014500723-A/160227: Polycomb-Associated Non-Coding RNAs.
MA588301 - JP 2018138019-A/160227: Polycomb-Associated Non-Coding RNAs.
LF352723 - JP 2014500723-A/160226: Polycomb-Associated Non-Coding RNAs.
MA588300 - JP 2018138019-A/160226: Polycomb-Associated Non-Coding RNAs.
BC015874 - Homo sapiens mannosidase, alpha, class 1A, member 2, mRNA (cDNA clone IMAGE:4714032), with apparent retained intron.
DL491797 - Novel nucleic acids.
DL490372 - Novel nucleic acids.
AK098129 - Homo sapiens cDNA FLJ40810 fis, clone TRACH2009743.
LF352722 - JP 2014500723-A/160225: Polycomb-Associated Non-Coding RNAs.
MA588299 - JP 2018138019-A/160225: Polycomb-Associated Non-Coding RNAs.
LF352721 - JP 2014500723-A/160224: Polycomb-Associated Non-Coding RNAs.
MA588298 - JP 2018138019-A/160224: Polycomb-Associated Non-Coding RNAs.

-  Biochemical and Signaling Pathways
  KEGG - Kyoto Encyclopedia of Genes and Genomes
hsa00510 - N-Glycan biosynthesis
hsa01100 - Metabolic pathways

BioCyc Knowledge Library
PWY-7919 - protein N-glycosylation processing phase (mammalian)

BioCarta from NCI Cancer Genome Anatomy Project
h_aMANpathway - Steps in the Glycosylation of Mammalian N-linked Oligosaccarides
h_eradPathway - ER¿associated degradation (ERAD) Pathway

Reactome (by CSHL, EBI, and GO)

Protein O60476 (Reactome details) participates in the following event(s):

R-HSA-964737 Progressive trimming of alpha-1,2-linked mannose residues from Man9GlcNAc2 to produce Man5GlcNAc2
R-HSA-964825 Progressive trimming of alpha-1,2-linked mannose residues from Man8GlcNAc2 to produce Man5GlcNAc2
R-HSA-964830 Progressive trimming of alpha-1,2-linked mannose residues from Man7GlcNAc2 to produce Man5GlcNAc2
R-HSA-8847544 The COG complex and CUX1 and GOLGA5 dimers contribute to intra-Golgi vesicle tethering
R-HSA-6811438 Intra-Golgi traffic
R-HSA-964827 Progressive trimming of alpha-1,2-linked mannose residues from Man9/8/7GlcNAc2 to produce Man5GlcNAc2
R-HSA-6811442 Intra-Golgi and retrograde Golgi-to-ER traffic
R-HSA-964739 N-glycan trimming and elongation in the cis-Golgi
R-HSA-199991 Membrane Trafficking
R-HSA-948021 Transport to the Golgi and subsequent modification
R-HSA-5653656 Vesicle-mediated transport
R-HSA-446203 Asparagine N-linked glycosylation
R-HSA-597592 Post-translational protein modification
R-HSA-392499 Metabolism of proteins

-  Other Names for This Gene
  Alternate Gene Symbols: ENST00000356554.1, ENST00000356554.2, ENST00000356554.3, ENST00000356554.4, ENST00000356554.5, ENST00000356554.6, MA1A2_HUMAN, MAN1B, NM_006699, O60476, Q9H510, uc001ehd.1, uc001ehd.2
UCSC ID: ENST00000356554.7
RefSeq Accession: NM_006699
Protein: O60476 (aka MA1A2_HUMAN or M1A2_HUMAN)
CCDS: CCDS895.1

-  Methods, Credits, and Use Restrictions
  Click here for details on how this gene model was made and data restrictions if any.