Human Gene CHRM4 (ENST00000433765.3) from GENCODE V44
  Description: The muscarinic acetylcholine receptor mediates various cellular responses, including inhibition of adenylate cyclase, breakdown of phosphoinositides and modulation of potassium channels through the action of G proteins. Primary transducing effect is inhibition of adenylate cyclase. (from UniProt P08173)
Gencode Transcript: ENST00000433765.3
Gencode Gene: ENSG00000180720.8
Transcript (Including UTRs)
   Position: hg38 chr11:46,385,098-46,386,608 Size: 1,511 Total Exon Count: 1 Strand: -
Coding Region
   Position: hg38 chr11:46,385,118-46,386,557 Size: 1,440 Coding Exon Count: 1 

Page IndexSequence and LinksUniProtKB CommentsPrimersCTDRNA-Seq Expression
Microarray ExpressionRNA StructureProtein StructureOther SpeciesGO AnnotationsmRNA Descriptions
PathwaysOther NamesMethods
Data last updated at UCSC: 2023-08-18 00:09:47

-  Sequence and Links to Tools and Databases
 
Genomic Sequence (chr11:46,385,098-46,386,608)mRNA (may differ from genome)Protein (479 aa)
Gene SorterGenome BrowserOther Species FASTAGene interactionsTable SchemaAlphaFold
EnsemblExonPrimerGencodeGeneCardsHGNCHPRD
LynxMalacardsMGImyGene2neXtProtPubMed
ReactomeUniProtKBWikipedia

-  Comments and Description Text from UniProtKB
  ID: ACM4_HUMAN
DESCRIPTION: RecName: Full=Muscarinic acetylcholine receptor M4;
FUNCTION: The muscarinic acetylcholine receptor mediates various cellular responses, including inhibition of adenylate cyclase, breakdown of phosphoinositides and modulation of potassium channels through the action of G proteins. Primary transducing effect is inhibition of adenylate cyclase.
SUBCELLULAR LOCATION: Cell membrane; Multi-pass membrane protein. Cell junction, synapse, postsynaptic cell membrane; Multi-pass membrane protein.
SIMILARITY: Belongs to the G-protein coupled receptor 1 family. Muscarinic acetylcholine receptor subfamily. CHRM4 sub-subfamily.

-  Primer design for this transcript
 

Primer3Plus can design qPCR Primers that straddle exon-exon-junctions, which amplify only cDNA, not genomic DNA.
Click here to load the transcript sequence and exon structure into Primer3Plus

Exonprimer can design one pair of Sanger sequencing primers around every exon, located in non-genic sequence.
Click here to open Exonprimer with this transcript

To design primers for a non-coding sequence, zoom to a region of interest and select from the drop-down menu: View > In External Tools > Primer3


-  Comparative Toxicogenomics Database (CTD)
  The following chemicals interact with this gene           more ... click here to view the complete list

-  RNA-Seq Expression Data from GTEx (53 Tissues, 570 Donors)
  Highest median expression: 9.03 RPKM in Brain - Caudate (basal ganglia)
Total median expression: 56.86 RPKM



View in GTEx track of Genome Browser    View at GTEx portal     View GTEx Body Map

+  Microarray Expression Data
  Press "+" in the title bar above to open this section.

-  mRNA Secondary Structure of 3' and 5' UTRs
 
RegionFold EnergyBasesEnergy/Base
Display As
5' UTR -4.2051-0.082 Picture PostScript Text
3' UTR -5.3020-0.265 Picture PostScript Text

The RNAfold program from the Vienna RNA Package is used to perform the secondary structure predictions and folding calculations. The estimated folding energy is in kcal/mol. The more negative the energy, the more secondary structure the RNA is likely to have.

-  Protein Domain and Structure Information
  InterPro Domains: Graphical view of domain structure
IPR000276 - 7TM_GPCR_Rhodpsn
IPR017452 - GPCR_Rhodpsn_supfam
IPR001432 - Musac_M4_rcpt
IPR000995 - Musac_rcpt

Pfam Domains:
PF00001 - 7 transmembrane receptor (rhodopsin family)

ModBase Predicted Comparative 3D Structure on P08173
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The pictures above may be empty if there is no ModBase structure for the protein. The ModBase structure frequently covers just a fragment of the protein. You may be asked to log onto ModBase the first time you click on the pictures. It is simplest after logging in to just click on the picture again to get to the specific info on that model.

-  Orthologous Genes in Other Species
  Orthologies between human, mouse, and rat are computed by taking the best BLASTP hit, and filtering out non-syntenic hits. For more distant species reciprocal-best BLASTP hits are used. Note that the absence of an ortholog in the table below may reflect incomplete annotations in the other species rather than a true absence of the orthologous gene.
MouseRatZebrafishD. melanogasterC. elegansS. cerevisiae
Genome BrowserGenome BrowserGenome BrowserNo orthologNo orthologNo ortholog
Gene Details     
Gene Sorter     
MGIRGDEnsembl   
Protein SequenceProtein SequenceProtein Sequence   
AlignmentAlignmentAlignment   

-  Gene Ontology (GO) Annotations with Structured Vocabulary
  Molecular Function:
GO:0004871 signal transducer activity
GO:0004930 G-protein coupled receptor activity
GO:0016907 G-protein coupled acetylcholine receptor activity

Biological Process:
GO:0007165 signal transduction
GO:0007166 cell surface receptor signaling pathway
GO:0007186 G-protein coupled receptor signaling pathway
GO:0007197 adenylate cyclase-inhibiting G-protein coupled acetylcholine receptor signaling pathway
GO:0007207 phospholipase C-activating G-protein coupled acetylcholine receptor signaling pathway
GO:0007213 G-protein coupled acetylcholine receptor signaling pathway
GO:0007271 synaptic transmission, cholinergic
GO:0008283 cell proliferation
GO:0040012 regulation of locomotion

Cellular Component:
GO:0005886 plasma membrane
GO:0005887 integral component of plasma membrane
GO:0016020 membrane
GO:0016021 integral component of membrane
GO:0030054 cell junction
GO:0045202 synapse
GO:0045211 postsynaptic membrane


-  Descriptions from all associated GenBank mRNAs
  LF205775 - JP 2014500723-A/13278: Polycomb-Associated Non-Coding RNAs.
MA441352 - JP 2018138019-A/13278: Polycomb-Associated Non-Coding RNAs.
BC119775 - Homo sapiens cholinergic receptor, muscarinic 4, mRNA (cDNA clone IMAGE:40109487), partial cds.
BC119817 - Homo sapiens cholinergic receptor, muscarinic 4, mRNA (cDNA clone IMAGE:40109488), partial cds.
BC137535 - Homo sapiens cholinergic receptor, muscarinic 4, mRNA (cDNA clone MGC:169158 IMAGE:9021535), complete cds.
AF498918 - Homo sapiens muscarinic acetylcholine receptor M4 (CHRM4) mRNA, complete cds.
BC095546 - Homo sapiens cholinergic receptor, muscarinic 4, mRNA (cDNA clone IMAGE:6519223), partial cds.
KJ890912 - Synthetic construct Homo sapiens clone ccsbBroadEn_00306 CHRM4 gene, encodes complete protein.
AF385590 - Homo sapiens m4 muscarinic cholinergic receptor (CHRM4) mRNA, complete cds.

-  Biochemical and Signaling Pathways
  Reactome (by CSHL, EBI, and GO)

Protein P08173 (Reactome details) participates in the following event(s):

R-HSA-390673 M2 and M4 receptors bind acetylcholine
R-HSA-749454 The Ligand:GPCR:Gi complex dissociates
R-HSA-749456 Liganded Gi-activating GPCRs bind inactive heterotrimeric G-protein Gi
R-HSA-380073 Liganded Gi-activating GPCR acts as a GEF for Gi
R-HSA-390648 Muscarinic acetylcholine receptors
R-HSA-418594 G alpha (i) signalling events
R-HSA-375280 Amine ligand-binding receptors
R-HSA-388396 GPCR downstream signalling
R-HSA-373076 Class A/1 (Rhodopsin-like receptors)
R-HSA-372790 Signaling by GPCR
R-HSA-500792 GPCR ligand binding
R-HSA-162582 Signal Transduction

-  Other Names for This Gene
  Alternate Gene Symbols: ACM4_HUMAN, B2RPP4, BC137535, ENST00000433765.1, ENST00000433765.2, P08173, Q0VD60, Q4VBK7, uc001nct.1, uc001nct.2
UCSC ID: ENST00000433765.3
Representative RNA: BC137535
Protein: P08173 (aka ACM4_HUMAN)
CCDS: CCDS44581.1

-  Methods, Credits, and Use Restrictions
  Click here for details on how this gene model was made and data restrictions if any.