Human Gene ARHGDIG (ENST00000219409.8) from GENCODE V44
  Description: Homo sapiens Rho GDP dissociation inhibitor gamma (ARHGDIG), mRNA. (from RefSeq NM_001176)
RefSeq Summary (NM_001176): The GDP-dissociation inhibitors (GDIs) play a primary role in modulating the activation of GTPases by inhibiting the exchange of GDP for GTP. See ARHGDIB (MIM 602843).[supplied by OMIM, Nov 2010].
Gencode Transcript: ENST00000219409.8
Gencode Gene: ENSG00000242173.10
Transcript (Including UTRs)
   Position: hg38 chr16:280,591-283,010 Size: 2,420 Total Exon Count: 6 Strand: +
Coding Region
   Position: hg38 chr16:280,681-282,814 Size: 2,134 Coding Exon Count: 6 

Page IndexSequence and LinksUniProtKB CommentsPrimersCTDRNA-Seq Expression
Microarray ExpressionRNA StructureProtein StructureOther SpeciesGO AnnotationsmRNA Descriptions
PathwaysOther NamesMethods
Data last updated at UCSC: 2023-08-18 00:09:47

-  Sequence and Links to Tools and Databases
 
Genomic Sequence (chr16:280,591-283,010)mRNA (may differ from genome)Protein (225 aa)
Gene SorterGenome BrowserOther Species FASTAGene interactionsTable SchemaAlphaFold
BioGPSEnsemblEntrez GeneExonPrimerGencodeGeneCards
HGNCHPRDLynxMalacardsMGIneXtProt
OMIMPubMedReactomeUniProtKBWikipediaBioGrid CRISPR DB

-  Comments and Description Text from UniProtKB
  ID: GDIR3_HUMAN
DESCRIPTION: RecName: Full=Rho GDP-dissociation inhibitor 3; Short=Rho GDI 3; AltName: Full=Rho-GDI gamma;
FUNCTION: Inhibits GDP/GTP exchange reaction of RhoB. Interacts specifically with the GDP- and GTP-bound forms of post- translationally processed Rhob and Rhog proteins, both of which show a growth-regulated expression in mammalian cells. Stimulates the release of the GDP-bound but not the GTP-bound RhoB protein. Also inhibits the GDP/GTP exchange of RhoB but shows less ability to inhibit the dissociation of prebound GTP.
SUBCELLULAR LOCATION: Cytoplasm.
TISSUE SPECIFICITY: Primarily expressed in pancreas and brain.
SIMILARITY: Belongs to the Rho GDI family.

-  Primer design for this transcript
 

Primer3Plus can design qPCR Primers that straddle exon-exon-junctions, which amplify only cDNA, not genomic DNA.
Click here to load the transcript sequence and exon structure into Primer3Plus

Exonprimer can design one pair of Sanger sequencing primers around every exon, located in non-genic sequence.
Click here to open Exonprimer with this transcript

To design primers for a non-coding sequence, zoom to a region of interest and select from the drop-down menu: View > In External Tools > Primer3


-  Comparative Toxicogenomics Database (CTD)
  The following chemicals interact with this gene           more ... click here to view the complete list

-  RNA-Seq Expression Data from GTEx (53 Tissues, 570 Donors)
  Highest median expression: 123.83 RPKM in Brain - Cerebellum
Total median expression: 915.30 RPKM



View in GTEx track of Genome Browser    View at GTEx portal     View GTEx Body Map

+  Microarray Expression Data
  Press "+" in the title bar above to open this section.

-  mRNA Secondary Structure of 3' and 5' UTRs
 
RegionFold EnergyBasesEnergy/Base
Display As
5' UTR -60.2090-0.669 Picture PostScript Text
3' UTR -41.40196-0.211 Picture PostScript Text

The RNAfold program from the Vienna RNA Package is used to perform the secondary structure predictions and folding calculations. The estimated folding energy is in kcal/mol. The more negative the energy, the more secondary structure the RNA is likely to have.

-  Protein Domain and Structure Information
  InterPro Domains: Graphical view of domain structure
IPR014756 - Ig_E-set
IPR000406 - Rho_GDI
IPR024792 - RhoGDI_domain

Pfam Domains:
PF02115 - RHO protein GDP dissociation inhibitor

ModBase Predicted Comparative 3D Structure on Q99819
FrontTopSide
The pictures above may be empty if there is no ModBase structure for the protein. The ModBase structure frequently covers just a fragment of the protein. You may be asked to log onto ModBase the first time you click on the pictures. It is simplest after logging in to just click on the picture again to get to the specific info on that model.

-  Orthologous Genes in Other Species
  Orthologies between human, mouse, and rat are computed by taking the best BLASTP hit, and filtering out non-syntenic hits. For more distant species reciprocal-best BLASTP hits are used. Note that the absence of an ortholog in the table below may reflect incomplete annotations in the other species rather than a true absence of the orthologous gene.
MouseRatZebrafishD. melanogasterC. elegansS. cerevisiae
Genome BrowserGenome BrowserNo orthologNo orthologNo orthologNo ortholog
Gene Details     
Gene Sorter     
MGIRGD    
Protein SequenceProtein Sequence    
AlignmentAlignment    

-  Gene Ontology (GO) Annotations with Structured Vocabulary
  Molecular Function:
GO:0005092 GDP-dissociation inhibitor activity
GO:0005094 Rho GDP-dissociation inhibitor activity
GO:0005096 GTPase activator activity
GO:0005515 protein binding
GO:0030695 GTPase regulator activity

Biological Process:
GO:0007162 negative regulation of cell adhesion
GO:0007266 Rho protein signal transduction
GO:0032880 regulation of protein localization
GO:0043547 positive regulation of GTPase activity
GO:0050790 regulation of catalytic activity
GO:0051056 regulation of small GTPase mediated signal transduction

Cellular Component:
GO:0005737 cytoplasm
GO:0005829 cytosol
GO:0005886 plasma membrane
GO:0016020 membrane
GO:0031410 cytoplasmic vesicle


-  Descriptions from all associated GenBank mRNAs
  AB593111 - Homo sapiens ARHGDIG mRNA for rho GDP-dissociation inhibitor 3, complete cds, clone: HP06965-RBd09D08.
BC047699 - Homo sapiens Rho GDP dissociation inhibitor (GDI) gamma, mRNA (cDNA clone MGC:51949 IMAGE:5581679), complete cds.
U82532 - Homo sapiens GDI-dissociation inhibitor RhoGDIgamma mRNA, complete cds.
AB590832 - Synthetic construct DNA, clone: pFN21AE0909, Homo sapiens ARHGDIG gene for Rho GDP dissociation inhibitor (GDI) gamma, without stop codon, in Flexi system.
CU690464 - Synthetic construct Homo sapiens gateway clone IMAGE:100021341 5' read ARHGDIG mRNA.
AF498928 - Homo sapiens Rho GDP dissociation inhibitor gamma (ARHGDIG) mRNA, complete cds.
AB127078 - Homo sapiens ARHGDIG, PDIP polycistronic mRNA for Rho GDP dissociation inhibitor gamma, pancreatic protein disulfide isomerase, complete cds.
JD124598 - Sequence 105622 from Patent EP1572962.
JD534319 - Sequence 515343 from Patent EP1572962.
JD439576 - Sequence 420600 from Patent EP1572962.
JD103266 - Sequence 84290 from Patent EP1572962.
JD392965 - Sequence 373989 from Patent EP1572962.
JD200251 - Sequence 181275 from Patent EP1572962.

-  Biochemical and Signaling Pathways
  Reactome (by CSHL, EBI, and GO)

Protein Q99819 (Reactome details) participates in the following event(s):

R-HSA-194854 GDIs block activation of Rho GTPase:GDP
R-HSA-195146 Dissociation of Rho GTP:GDP from GDI complex
R-HSA-194840 Rho GTPase cycle
R-HSA-194315 Signaling by Rho GTPases
R-HSA-162582 Signal Transduction

-  Other Names for This Gene
  Alternate Gene Symbols: ENST00000219409.1, ENST00000219409.2, ENST00000219409.3, ENST00000219409.4, ENST00000219409.5, ENST00000219409.6, ENST00000219409.7, GDIR3_HUMAN, NM_001176, Q4TT69, Q96S29, Q99819, uc002cgm.1, uc002cgm.2, uc002cgm.3
UCSC ID: ENST00000219409.8
RefSeq Accession: NM_001176
Protein: Q99819 (aka GDIR3_HUMAN)
CCDS: CCDS10404.1

-  Methods, Credits, and Use Restrictions
  Click here for details on how this gene model was made and data restrictions if any.